##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765492_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7381578 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37905458697314 33.0 33.0 33.0 33.0 33.0 2 32.33459105898495 33.0 33.0 33.0 33.0 33.0 3 32.38648849880067 33.0 33.0 33.0 33.0 33.0 4 36.25973416524217 37.0 37.0 37.0 33.0 37.0 5 36.18889511158725 37.0 37.0 37.0 33.0 37.0 6 36.193652766386805 37.0 37.0 37.0 33.0 37.0 7 36.08702421081237 37.0 37.0 37.0 33.0 37.0 8 36.10064866888896 37.0 37.0 37.0 33.0 37.0 9 36.13129143931013 37.0 37.0 37.0 33.0 37.0 10-11 36.087497144919425 37.0 37.0 37.0 33.0 37.0 12-13 36.059201094942026 37.0 37.0 37.0 33.0 37.0 14-15 37.56626665734616 40.0 37.0 40.0 33.0 40.0 16-17 37.56638573757536 40.0 37.0 40.0 33.0 40.0 18-19 37.618417633736314 40.0 37.0 40.0 33.0 40.0 20-21 37.59758150628497 40.0 37.0 40.0 33.0 40.0 22-23 37.575164280591494 40.0 37.0 40.0 33.0 40.0 24-25 37.55209346836138 40.0 37.0 40.0 33.0 40.0 26-27 37.42743387660471 40.0 37.0 40.0 33.0 40.0 28-29 37.344099391756075 40.0 37.0 40.0 33.0 40.0 30-31 37.20245847161677 37.0 37.0 40.0 33.0 40.0 32-33 37.10654056625833 37.0 37.0 40.0 33.0 40.0 34-35 37.02167774966274 37.0 37.0 40.0 33.0 40.0 36-37 36.892952021911846 37.0 37.0 40.0 33.0 40.0 38-39 36.79968185664366 37.0 37.0 40.0 33.0 40.0 40-41 36.651720404498874 37.0 37.0 40.0 33.0 40.0 42-43 36.51470268281389 37.0 37.0 40.0 33.0 40.0 44-45 36.24821589638422 37.0 37.0 40.0 33.0 40.0 46-47 36.03213093460504 37.0 37.0 40.0 33.0 40.0 48-49 35.76798145328817 37.0 37.0 40.0 27.0 40.0 50-51 35.575960180871895 37.0 37.0 40.0 27.0 40.0 52-53 35.42483443242082 37.0 37.0 40.0 27.0 40.0 54-55 35.24657119385584 37.0 33.0 40.0 27.0 40.0 56-57 35.05156411000466 37.0 33.0 40.0 27.0 40.0 58-59 34.83612331943116 37.0 33.0 40.0 27.0 40.0 60-61 34.67143387226959 37.0 33.0 38.5 27.0 40.0 62-63 34.451508810175824 37.0 33.0 37.0 27.0 40.0 64-65 34.213619906204336 37.0 33.0 37.0 27.0 40.0 66-67 33.980181541128474 37.0 33.0 37.0 27.0 40.0 68-69 33.758587662421235 37.0 33.0 37.0 27.0 40.0 70-71 33.56389778716692 37.0 33.0 37.0 27.0 38.5 72-73 33.351036390863854 37.0 33.0 37.0 27.0 37.0 74-75 33.10942043828569 37.0 33.0 37.0 27.0 37.0 76-77 32.92548205817239 37.0 33.0 37.0 27.0 37.0 78-79 32.749826459870775 37.0 33.0 37.0 27.0 37.0 80-81 32.5812239605136 33.0 33.0 37.0 24.5 37.0 82-83 32.46861504410033 33.0 33.0 37.0 22.0 37.0 84-85 32.33943473875098 33.0 33.0 37.0 22.0 37.0 86-87 32.22652615741512 33.0 33.0 37.0 22.0 37.0 88-89 32.1156365888161 33.0 33.0 37.0 22.0 37.0 90-91 32.00658599015007 33.0 33.0 37.0 22.0 37.0 92-93 31.929656843021913 33.0 33.0 37.0 22.0 37.0 94-95 31.823613257219527 33.0 33.0 37.0 22.0 37.0 96-97 31.758873441424043 33.0 33.0 37.0 22.0 37.0 98-99 30.972781632870372 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0022646439153675146 1101 2 0.08219102893734487 1101 3 0.0846430539857792 1101 4 0.08950059088214601 1101 5 0.08981799341258068 1101 6 0.09681467590796444 1101 7 0.13952793034749078 1101 8 0.14958893512004323 1101 9 0.08543600627066894 1101 10-11 0.07567482981298923 1101 12-13 0.09262518764302996 1101 14-15 0.17938787594983552 1101 16-17 0.24267711921024215 1101 18-19 0.19356004605408828 1101 20-21 0.230269476422869 1101 22-23 0.16834542075490333 1101 24-25 0.275401048326664 1101 26-27 0.2035571235365552 1101 28-29 0.22347132861888497 1101 30-31 0.25176885998288867 1101 32-33 0.2695333532175894 1101 34-35 0.3118538320597395 1101 36-37 0.3482748275537446 1101 38-39 0.24173268926652725 1101 40-41 0.19973057275517903 1101 42-43 0.22980148919215537 1101 44-45 0.3079455806888092 1101 46-47 0.32175589780938196 1101 48-49 0.3018405742502992 1101 50-51 0.3981845384021341 1101 52-53 0.4491174957555373 1101 54-55 0.38103437930281103 1101 56-57 0.46263181807622544 1101 58-59 0.4277424169285098 1101 60-61 0.3280795859710395 1101 62-63 0.3352223571180275 1101 64-65 0.3328410256762453 1101 66-67 0.4518373878003814 1101 68-69 0.467755201590478 1101 70-71 0.37078156851210764 1101 72-73 0.3650438936183207 1101 74-75 0.4060032259071562 1101 76-77 0.47309276295333547 1101 78-79 0.4007032798545609 1101 80-81 0.3814847536318524 1101 82-83 0.42135696294809577 1101 84-85 0.3641157413671223 1101 86-87 0.4013224751123943 1101 88-89 0.4027535625027099 1101 90-91 0.4232821352794076 1101 92-93 0.36522105178803344 1101 94-95 0.39996389154686796 1101 96-97 0.42272052344318567 1101 98-99 0.4723073961033073 1102 1 0.047113885190654514 1102 2 0.1310764369434949 1102 3 0.07097003727242424 1102 4 0.08962220185008363 1102 5 0.0394624700267201 1102 6 0.08402860534501855 1102 7 0.03377123983471364 1102 8 0.1472531208090757 1102 9 0.06544964982709445 1102 10-11 0.05470528643272843 1102 12-13 0.03224406082424025 1102 14-15 0.04389947202828637 1102 16-17 0.07333295113661364 1102 18-19 0.08604742523054654 1102 20-21 0.17520403917653482 1102 22-23 0.13938494772790477 1102 24-25 0.20690596824469054 1102 26-27 0.15537701315120955 1102 28-29 0.09624427797683666 1102 30-31 0.10944647322452994 1102 32-33 0.09109079655704022 1102 34-35 0.13662142879392292 1102 36-37 0.11944865879859634 1102 38-39 0.13838218267188296 1102 40-41 0.08513225484174569 1102 42-43 0.1419012096930814 1102 44-45 0.13995988769724477 1102 46-47 0.1471968802702861 1102 48-49 0.06825056049257938 1102 50-51 0.11716253484110695 1102 52-53 0.1069156070937538 1102 54-55 -0.02305145601308567 1102 56-57 0.04283676873578912 1102 58-59 0.1498455614594505 1102 60-61 0.07737180800226184 1102 62-63 0.1486092180578069 1102 64-65 0.11797361875438384 1102 66-67 0.16860605946152418 1102 68-69 0.13012974382459674 1102 70-71 0.05403704739132564 1102 72-73 0.022947785226783424 1102 74-75 0.11561173665449331 1102 76-77 0.1369399789393313 1102 78-79 0.13821402563359442 1102 80-81 0.11655617574427879 1102 82-83 0.08665599558774773 1102 84-85 0.06660719751288724 1102 86-87 0.09189282866299209 1102 88-89 0.11966039857987454 1102 90-91 -0.011203760451856226 1102 92-93 0.09919337176179255 1102 94-95 0.12516783240913298 1102 96-97 0.25706582779543297 1102 98-99 0.14098425201751397 1103 1 0.017543786896879965 1103 2 0.08896385206419666 1103 3 0.0751377741869419 1103 4 0.03411745116083864 1103 5 0.017170514702662842 1103 6 0.06210983511814305 1103 7 0.017403105365346505 1103 8 0.10891004778289926 1103 9 0.04892240943867421 1103 10-11 0.07532363385484331 1103 12-13 0.023492262262799102 1103 14-15 0.131847557989083 1103 16-17 0.13245730092318553 1103 18-19 0.15520482680092584 1103 20-21 0.11091682182391338 1103 22-23 0.17467054907019985 1103 24-25 0.17845939804553979 1103 26-27 0.17823341335719078 1103 28-29 0.08587824150279033 1103 30-31 0.1620556924047989 1103 32-33 0.09381858614511174 1103 34-35 0.14738996997497367 1103 36-37 0.13140977571503498 1103 38-39 0.06819205416704222 1103 40-41 0.12039705952881974 1103 42-43 0.12907593543037876 1103 44-45 0.16254957312149543 1103 46-47 0.12877570694164575 1103 48-49 0.16382090119193293 1103 50-51 0.17408522094371648 1103 52-53 0.07002186431515156 1103 54-55 0.031752236239050546 1103 56-57 0.041710059168764246 1103 58-59 0.14481523371046734 1103 60-61 0.09252497127001647 1103 62-63 0.10798027730159276 1103 64-65 0.14202836414251863 1103 66-67 0.1784206514865474 1103 68-69 0.18889900818501104 1103 70-71 -0.06037013327262031 1103 72-73 0.030479440675257763 1103 74-75 0.13304402922915415 1103 76-77 0.16918318829240775 1103 78-79 0.061297037112005626 1103 80-81 0.09761110515717775 1103 82-83 0.06486599828230766 1103 84-85 0.07965427450282192 1103 86-87 0.1369382264168877 1103 88-89 0.17306489703650385 1103 90-91 0.12822492139387975 1103 92-93 0.12335095784141714 1103 94-95 0.04436111436680079 1103 96-97 0.01687278509417567 1103 98-99 0.004547862045544804 1104 1 0.015157828623721059 1104 2 -0.02334042685702542 1104 3 -0.06271751831298644 1104 4 -0.043143463417486316 1104 5 -0.06283120062204972 1104 6 0.019970452191202526 1104 7 0.019502474374469614 1104 8 0.02935927474008082 1104 9 0.028225722852461388 1104 10-11 -0.0531325520417667 1104 12-13 -0.05275125921817647 1104 14-15 0.01225002314883028 1104 16-17 0.029183024026650628 1104 18-19 0.032284967579840895 1104 20-21 0.047591399952573 1104 22-23 0.05782259568998427 1104 24-25 0.06412394282337885 1104 26-27 0.04466288728224299 1104 28-29 -0.009380151344920762 1104 30-31 -0.10184739797644227 1104 32-33 0.018503529339540137 1104 34-35 -0.06658739023951199 1104 36-37 0.05492512543101924 1104 38-39 -0.03511329604965141 1104 40-41 -0.0038905864217113617 1104 42-43 0.10628382853447249 1104 44-45 0.039167925803624826 1104 46-47 -0.03810443418736753 1104 48-49 -0.001321884787905958 1104 50-51 -0.1396849095885031 1104 52-53 8.892204669592729E-4 1104 54-55 -0.15966729716794248 1104 56-57 -0.01887687309487518 1104 58-59 -0.03666496745007919 1104 60-61 -0.03539518372034678 1104 62-63 -0.1220125197705002 1104 64-65 -0.1020758061140441 1104 66-67 0.009220155349197512 1104 68-69 0.005421241052815162 1104 70-71 -0.16138865410885472 1104 72-73 -0.051117537120063616 1104 74-75 -0.025235723533370447 1104 76-77 -0.05292631471701981 1104 78-79 -0.07477105950442109 1104 80-81 -0.03976255491377856 1104 82-83 -0.02083533595136089 1104 84-85 7.775626237815914E-5 1104 86-87 0.04873097653536007 1104 88-89 0.050546522536087934 1104 90-91 0.012796652511859463 1104 92-93 0.08817464453968427 1104 94-95 -0.021130038057862066 1104 96-97 0.003726544232240059 1104 98-99 0.05983860018669773 1105 1 -0.07011209795616935 1105 2 0.09103000664159566 1105 3 0.04527458903134374 1105 4 0.06286731824744862 1105 5 0.0702288896288934 1105 6 0.018348482961336288 1105 7 0.04461429988269572 1105 8 0.06924208360928219 1105 9 -0.0031495271134716063 1105 10-11 0.013416910116767156 1105 12-13 -0.006599985504998074 1105 14-15 0.02456983003626334 1105 16-17 0.04835235753940026 1105 18-19 0.03376485047056832 1105 20-21 0.04624521071988852 1105 22-23 0.007710378616202718 1105 24-25 0.020546045090775067 1105 26-27 -0.045280034762498644 1105 28-29 -0.03339502795343918 1105 30-31 0.008682766812420084 1105 32-33 0.0032317003920638854 1105 34-35 0.0201254802865094 1105 36-37 0.04125630653533818 1105 38-39 -0.01266727749177221 1105 40-41 -0.04813029164972704 1105 42-43 -0.04176236526002697 1105 44-45 -0.021847685912973702 1105 46-47 -0.05890684489025233 1105 48-49 -0.04374600386275773 1105 50-51 -0.029605512993931882 1105 52-53 -0.058391069067482704 1105 54-55 -0.07451001087910925 1105 56-57 -0.058105347458415224 1105 58-59 0.02219994010904003 1105 60-61 -0.035445157597528976 1105 62-63 -0.0274499921344713 1105 64-65 -0.04536953753851236 1105 66-67 0.018613922452125564 1105 68-69 -0.04855661786005783 1105 70-71 -0.048783351143136144 1105 72-73 0.006274216026049828 1105 74-75 -0.01526848309796236 1105 76-77 -0.051542915808106216 1105 78-79 -0.02727119182451787 1105 80-81 -0.03336075871440869 1105 82-83 0.0021131645269463206 1105 84-85 -0.02634830816270295 1105 86-87 0.03595832319010839 1105 88-89 0.01735259689112212 1105 90-91 0.021462394343295443 1105 92-93 -0.017571835353685117 1105 94-95 0.015874877465382298 1105 96-97 -0.05390162930703113 1105 98-99 0.1299516711799491 1106 1 -0.03686703084741083 1106 2 0.009693204863815197 1106 3 0.015629191635078143 1106 4 0.02225317224744572 1106 5 0.010751249122755269 1106 6 0.08204171866294985 1106 7 -0.023520858039645987 1106 8 0.035792917538834956 1106 9 -0.022138513854578434 1106 10-11 0.01475200978038771 1106 12-13 -0.0117787838936394 1106 14-15 0.0701292056775813 1106 16-17 0.025632181819325695 1106 18-19 -6.978240033888028E-4 1106 20-21 -0.00389959470815171 1106 22-23 0.053843436361781016 1106 24-25 0.017490463906796094 1106 26-27 0.009766778212494387 1106 28-29 -0.04733499090537663 1106 30-31 0.044604273044186016 1106 32-33 0.02012545036426161 1106 34-35 0.13071676573279944 1106 36-37 0.09486243074209 1106 38-39 -0.01343049774475702 1106 40-41 0.03143217216825889 1106 42-43 0.009033386649868191 1106 44-45 0.05726901354839953 1106 46-47 0.04870064990523559 1106 48-49 9.02682675707922E-4 1106 50-51 -0.031015432180254265 1106 52-53 -0.009469016031538047 1106 54-55 -0.0683331082201164 1106 56-57 0.00686421081007893 1106 58-59 0.0703637012861762 1106 60-61 -0.018696110539686117 1106 62-63 -0.03600260129173449 1106 64-65 0.014754975980821428 1106 66-67 -0.031011037968887933 1106 68-69 0.023856858164606365 1106 70-71 -0.08214468038597289 1106 72-73 0.03897553116068764 1106 74-75 0.0117966214147458 1106 76-77 0.04149205238653764 1106 78-79 -0.01017976063470627 1106 80-81 -0.020150143943652665 1106 82-83 -0.050165376397579564 1106 84-85 -0.06694772313831976 1106 86-87 0.047486173742939286 1106 88-89 0.05819549929750423 1106 90-91 -0.07563920311019245 1106 92-93 -0.006164324272049271 1106 94-95 0.009905122099809205 1106 96-97 0.04859958876841475 1106 98-99 0.07717450364878431 1107 1 -0.04368690856880164 1107 2 0.004000490026555781 1107 3 0.010377817814614332 1107 4 -0.041344881681929735 1107 5 7.035138739013291E-4 1107 6 -0.015325166822101721 1107 7 0.00942490326119838 1107 8 0.031096952885832252 1107 9 0.016342550526907473 1107 10-11 -0.05490047760795136 1107 12-13 -0.05032557016574657 1107 14-15 -0.006631995875913788 1107 16-17 -0.09150987064362681 1107 18-19 -0.12755963236736534 1107 20-21 -0.08205699500830832 1107 22-23 -0.06166421846272385 1107 24-25 -0.10925385846270075 1107 26-27 -0.07807831899556561 1107 28-29 -0.097858627491199 1107 30-31 -0.08114616325598689 1107 32-33 -0.03243184148141154 1107 34-35 -0.08268530635888283 1107 36-37 -0.04462420529763733 1107 38-39 -0.12878919949874756 1107 40-41 -0.10830140786084286 1107 42-43 -0.0615476467991769 1107 44-45 -0.12288622148086858 1107 46-47 -0.02948533426193478 1107 48-49 -0.025215047723243345 1107 50-51 -0.016357121091552074 1107 52-53 -0.047602873306964 1107 54-55 -0.08221619886744236 1107 56-57 -0.04358843391364786 1107 58-59 -7.59318178346291E-4 1107 60-61 -0.001124612683952364 1107 62-63 -0.0374657248572845 1107 64-65 -0.09396043028434065 1107 66-67 -0.07790259493995677 1107 68-69 -0.14466371190883365 1107 70-71 -0.07951097395394413 1107 72-73 -0.12886232938365083 1107 74-75 0.0022004043377563676 1107 76-77 -0.02848218967317706 1107 78-79 0.009441622465480748 1107 80-81 -0.06557030960406252 1107 82-83 -0.08438295748067759 1107 84-85 0.016579186176159055 1107 86-87 -0.029479506522548604 1107 88-89 0.016132229089258487 1107 90-91 0.003813502714656636 1107 92-93 0.1160148089322135 1107 94-95 0.0321527267450179 1107 96-97 0.04731512869226506 1107 98-99 -0.012301611542099522 1108 1 -0.07228878869410238 1108 2 0.02426559324069899 1108 3 0.02029402636041766 1108 4 -0.07411294546380987 1108 5 0.03621043807927293 1108 6 0.005908661431789142 1108 7 -0.06627659786597917 1108 8 -0.0355501888081875 1108 9 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-0.2181264803054006 2215 64-65 -0.15893267385003185 2215 66-67 -0.20033808165183586 2215 68-69 -0.19046133925274944 2215 70-71 -0.26298425944954573 2215 72-73 -0.2995531259388571 2215 74-75 -0.3953285965412334 2215 76-77 -0.31044461297496895 2215 78-79 -0.20252127734161718 2215 80-81 -0.23925534640133606 2215 82-83 -0.20748142475029852 2215 84-85 -0.19178254602275047 2215 86-87 -0.2849440438912012 2215 88-89 -0.1915809453465691 2215 90-91 -0.2513626000335023 2215 92-93 -0.19324543359197577 2215 94-95 -0.15563410179731108 2215 96-97 -0.2547013396716089 2215 98-99 -0.2169691725071239 2216 1 -0.05130105226139392 2216 2 -0.06942721786712269 2216 3 -0.0625486103052566 2216 4 -0.16472782852076762 2216 5 -0.07515075966578877 2216 6 -0.12020013814579755 2216 7 -0.10520949941293622 2216 8 -0.13674693352678702 2216 9 -0.08110697017347945 2216 10-11 -0.06347436157432895 2216 12-13 -0.0492812122806896 2216 14-15 -0.23901318590340992 2216 16-17 -0.2165672432657928 2216 18-19 -0.10608701768518358 2216 20-21 -0.15943035185120635 2216 22-23 -0.15416884370499417 2216 24-25 -0.2563586529802322 2216 26-27 -0.24646685496075804 2216 28-29 -0.17674091832835614 2216 30-31 -0.1429020357422317 2216 32-33 -0.1255784455466582 2216 34-35 -0.1582058938417248 2216 36-37 -0.14596461472115863 2216 38-39 -0.13751140950977003 2216 40-41 -0.12339464137462386 2216 42-43 -0.15909599814703057 2216 44-45 -0.17993588743448186 2216 46-47 -0.08780902875452767 2216 48-49 -0.014887925686913661 2216 50-51 -0.08998489248346431 2216 52-53 -0.08858968641194309 2216 54-55 0.0055347262811054065 2216 56-57 -0.08488014667259591 2216 58-59 -0.0880208444502486 2216 60-61 -0.13835397945916128 2216 62-63 -0.08460098732002308 2216 64-65 -0.16246211002499678 2216 66-67 -0.14934902560615626 2216 68-69 -0.025053335271380206 2216 70-71 -0.08316911920476144 2216 72-73 -0.14800258482593875 2216 74-75 -0.09102581274855481 2216 76-77 -0.06226318360081251 2216 78-79 0.043061929010654865 2216 80-81 -0.15769794907498635 2216 82-83 -0.12612318161425407 2216 84-85 -0.127450317684783 2216 86-87 -0.16978537506517455 2216 88-89 -0.17052114938947938 2216 90-91 -0.1384424444400736 2216 92-93 -0.023854979637867757 2216 94-95 -0.08816046451400439 2216 96-97 -0.09390862173162162 2216 98-99 -0.028698740989227645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 1.0 9 17.0 10 60.0 11 132.0 12 247.0 13 464.0 14 1083.0 15 2747.0 16 6187.0 17 12119.0 18 20065.0 19 29571.0 20 39901.0 21 52046.0 22 64219.0 23 63921.0 24 54712.0 25 51681.0 26 60322.0 27 77191.0 28 102687.0 29 134612.0 30 175888.0 31 233036.0 32 306004.0 33 404300.0 34 541094.0 35 770232.0 36 1289500.0 37 2092005.0 38 794748.0 39 785.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.03785457701523 18.444450137493924 12.950481177897357 25.567214107593482 2 16.06099671371081 20.765546337111115 37.42506006168328 25.74839688749479 3 18.236100736184053 25.37592910350605 29.650164233176156 26.73780592713374 4 13.37325162722659 17.11944248235269 36.255892710203696 33.25141318021702 5 14.13765820641144 36.89819242993668 33.69618407896841 15.267965284683479 6 31.918974506535054 36.837570503217606 17.050554772976724 14.192900217270616 7 27.366424902642766 31.777812278079292 22.06996932092298 18.78579349835496 8 27.11811756239655 32.957031680759854 19.869315206044018 20.055535550799572 9 27.13056747486784 14.106048869225523 19.444257040974165 39.31912661493247 10-11 25.405170818488948 25.02822567207175 27.529608167792848 22.036995341646463 12-13 26.84130683168287 22.851563717134738 27.21970830627272 23.087421144909666 14-15 24.06691001390504 23.28282776282748 25.558931414444984 27.091330808822494 16-17 23.354964993317175 25.98999205214142 26.074158201335862 24.580884753205545 18-19 23.492494469468262 25.018419300568507 28.505321428564173 22.98376480139906 20-21 24.560491326603913 24.436391652097154 27.972395832239677 23.03072118905926 22-23 24.330725760806157 24.072095424582656 27.805104816341437 23.792073998269746 24-25 23.852237285848634 24.446209198087455 27.882595022365138 23.818958493698773 26-27 23.975483290971116 24.48916749237087 27.69812904503617 23.83722017162184 28-29 23.77563442396734 24.497512591481115 27.990810366022007 23.736042618529535 30-31 24.05588110380387 24.282770659067555 27.939183628404002 23.722164608724572 32-33 23.918327490409233 24.469022748252474 27.656518701014875 23.956131060323415 34-35 23.993020191617564 24.50327694159704 27.835694481586458 23.668008385198938 36-37 24.174898646332803 24.26107940605654 27.57184845841905 23.992173489191607 38-39 24.21130685064901 24.245933593060993 27.608595343705638 23.934164212584356 40-41 24.111856570505655 24.104493646209523 27.693177529249162 24.090472254035657 42-43 24.230476193572702 24.204838044114688 27.282973911540324 24.281711850772286 44-45 24.22940596170629 24.495609204427563 26.97982734856964 24.295157485296503 46-47 24.208008097997475 24.59040600803785 26.707107883978193 24.494478009986484 48-49 24.702638108003462 24.581681586240776 26.23259552361297 24.48308478214279 50-51 24.159285453598134 25.02906560087829 25.97437160455393 24.837277340969642 52-53 24.182959253427928 25.146066328906908 25.71498262295677 24.955991794708392 54-55 24.19087084089608 25.041888062417005 25.758482806792806 25.00875828989411 56-57 24.28457709178173 24.871070928194484 25.812847876158727 25.031504103865053 58-59 24.01665823012198 24.877971528168814 26.144760539974083 24.960609701735123 60-61 24.149538215270503 24.992352583688746 25.89258692382577 24.96552227721498 62-63 23.98954532486143 24.831038837495182 25.833263565053432 25.346152272589954 64-65 23.690442612677128 24.753115119829392 26.045955214454146 25.510487053039338 66-67 24.197861216124792 24.669853790070363 25.89442257468525 25.2378624191196 68-69 23.962626961335367 24.503209205402964 25.98272347728358 25.551440355978084 70-71 23.774706438108492 24.592905473599277 25.820353046462426 25.812035041829812 72-73 24.107643379234087 24.602429182486453 25.85298834476856 25.4369390935109 74-75 24.113241415280616 24.703677738152088 25.732035507327367 25.45104533923993 76-77 23.87461741696462 24.64461647204337 25.744836716591703 25.73592939440031 78-79 23.890714380061166 24.726308409790594 25.691210886565536 25.691766323582705 80-81 23.700044042079355 24.747516689184437 25.80520395574008 25.747235312996125 82-83 23.800273302032885 24.561574037634507 25.800814649769283 25.837338010563325 84-85 23.873119008793143 24.597628383318565 25.518783528710177 26.010469079178115 86-87 23.530541773752887 24.57819284474606 25.822118040027835 26.06914734147322 88-89 23.681447771319107 24.215718847762716 25.883640331759477 26.219193049158697 90-91 23.72532430717042 24.33589085152689 25.729651338040142 26.209133503262542 92-93 23.681026700574094 24.33751513333285 25.66627476191905 26.315183404174007 94-95 23.19135254762123 24.241477362978962 26.081813820179043 26.485356269220762 96-97 23.461823949616207 24.147247528626263 25.91263931430005 26.47828920745748 98-99 23.383278283748066 24.280897434907438 25.7093552594199 26.6264690219246 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 131.0 1 150.5 2 509.0 3 845.5 4 591.5 5 515.5 6 1021.5 7 2764.5 8 4975.0 9 4887.5 10 2931.0 11 1745.0 12 1641.5 13 1803.0 14 2063.5 15 2503.0 16 2993.5 17 3535.0 18 4373.0 19 5407.5 20 6707.5 21 8324.0 22 10463.0 23 13217.0 24 16436.0 25 20493.5 26 25468.0 27 30328.0 28 36784.0 29 44928.5 30 51870.0 31 60247.0 32 69667.5 33 78827.5 34 88640.5 35 98438.0 36 106510.5 37 118423.5 38 134702.0 39 142213.0 40 149730.0 41 161699.5 42 173697.0 43 191202.0 44 214101.5 45 241098.0 46 264458.0 47 286479.5 48 304845.5 49 322200.0 50 341877.0 51 344795.5 52 340660.0 53 340693.0 54 326436.0 55 310243.0 56 291872.5 57 266542.5 58 246287.5 59 216175.0 60 178472.5 61 144183.0 62 117208.5 63 93603.0 64 69412.5 65 50746.5 66 39391.5 67 30976.0 68 23802.0 69 19375.5 70 15767.5 71 12493.0 72 10798.0 73 8825.0 74 6544.0 75 5066.5 76 3779.5 77 2635.0 78 1834.0 79 1260.0 80 870.0 81 589.5 82 346.0 83 209.5 84 132.5 85 72.0 86 38.0 87 19.5 88 7.5 89 5.5 90 4.0 91 4.0 92 2.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05637006071059602 2 0.0 3 0.0 4 0.0 5 0.0053918010485020955 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0011108735828572155 16-17 0.004667023771881839 18-19 7.383245154355885E-4 20-21 1.5579324637631683E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 1.7611410459931467E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 2.0320858222997848E-5 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.0641716445995696E-5 76-77 1.4224600756098494E-4 78-79 4.0641716445995696E-5 80-81 1.35472388153319E-5 82-83 1.35472388153319E-5 84-85 4.1996440327528886E-4 86-87 8.805705229965734E-4 88-89 3.454545897909634E-4 90-91 9.889484335192287E-4 92-93 0.001456328172648179 94-95 0.0010092692917422264 96-97 0.002126916494007108 98-99 6.909091795819268E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7381578.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.804895886383164 #Duplication Level Percentage of deduplicated Percentage of total 1 72.67858647093689 32.563564999998235 2 15.204753741798417 13.624948167587458 3 5.149783006698843 6.922064743578208 4 2.2652016352905315 4.059684937234287 5 1.2289172754486213 2.753075528972657 6 0.7289361560297638 1.9595945147240355 7 0.5010534022232185 1.571475186409056 8 0.33875908384854936 1.214245238592064 9 0.24878692695664956 1.003218512416731 >10 1.3858113255067774 11.572261016523983 >50 0.13554874633715455 4.277459888767392 >100 0.11757649395049541 10.57224635544316 >500 0.01160260353014107 3.5128257273860672 >1k 0.004501848937277081 3.4224578853100187 >5k 6.0427502281583014E-5 0.20756649696412496 >10k+ 1.2085500456316603E-4 0.7633108000925553 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21212 0.2873640297508202 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13454 0.18226455102147537 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10951 0.1483558122666996 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 10769 0.1458902148023092 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 9974 0.13512015994412035 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 1.3547238815331898E-5 0.0 1.3547238815331898E-5 0.0 6 0.0 1.3547238815331898E-5 0.0 1.3547238815331898E-5 0.0 7 0.0 1.3547238815331898E-5 0.0 1.3547238815331898E-5 0.0 8 0.0 1.3547238815331898E-5 0.0 1.3547238815331898E-5 0.0 9 0.0 1.3547238815331898E-5 0.0 1.3547238815331898E-5 0.0 10-11 0.0 1.3547238815331898E-5 0.0 2.7094477630663795E-5 0.0 12-13 6.773619407665949E-6 2.7094477630663795E-5 0.0 3.3868097038329746E-5 0.0 14-15 2.0320858222997845E-5 2.7094477630663795E-5 0.0 4.0641716445995696E-5 0.0 16-17 2.7094477630663795E-5 2.7094477630663795E-5 0.0 5.418895526132759E-5 0.0 18-19 2.7094477630663795E-5 2.7094477630663795E-5 0.0 5.418895526132759E-5 0.0 20-21 4.0641716445995696E-5 4.0641716445995696E-5 0.0 7.450981348432543E-5 1.3547238815331898E-5 22-23 6.096257466899354E-5 4.0641716445995696E-5 0.0 4.2673802268295476E-4 1.3547238815331898E-5 24-25 8.805705229965734E-5 4.0641716445995696E-5 0.0 0.0016324422772474936 1.3547238815331898E-5 26-27 1.3547238815331898E-4 4.0641716445995696E-5 0.0 0.00296007168115002 2.7094477630663795E-5 28-29 1.3547238815331898E-4 4.0641716445995696E-5 0.0 0.010756507619373527 4.0641716445995696E-5 30-31 1.6934048519164871E-4 4.0641716445995696E-5 0.0 0.036279505547458825 4.0641716445995696E-5 32-33 2.0320858222997847E-4 4.0641716445995696E-5 0.0 0.0840877113267651 4.0641716445995696E-5 34-35 2.099822016376444E-4 4.0641716445995696E-5 0.0 0.14713656077331974 4.0641716445995696E-5 36-37 2.2352944045297632E-4 6.096257466899355E-5 0.0 0.25081357942705473 4.0641716445995696E-5 38-39 2.3030305986064227E-4 8.128343289199139E-5 0.0 0.4174242959974141 5.418895526132759E-5 40-41 2.4385029867597416E-4 8.128343289199139E-5 0.0 0.607343036949552 6.773619407665949E-5 42-43 2.9126563452963584E-4 8.128343289199139E-5 0.0 0.7857398512892501 6.773619407665949E-5 44-45 3.3190735097563147E-4 8.805705229965734E-5 0.0 0.9922268653125389 6.773619407665949E-5 46-47 3.3868097038329743E-4 9.483067170732328E-5 0.0 1.2209381246123796 6.773619407665949E-5 48-49 3.3868097038329743E-4 9.483067170732328E-5 0.0 1.4707966237029535 7.450981348432545E-5 50-51 3.3868097038329743E-4 9.483067170732328E-5 0.0 1.7521795475168047 8.128343289199139E-5 52-53 3.3868097038329743E-4 1.0160429111498922E-4 0.0 2.014393128406961 8.128343289199139E-5 54-55 3.3868097038329743E-4 1.2869876874565302E-4 0.0 2.2762273866102882 8.128343289199139E-5 56-57 3.3868097038329743E-4 1.3547238815331898E-4 1.3547238815331898E-5 2.577477336146824 8.128343289199139E-5 58-59 3.3868097038329743E-4 1.4901962696865087E-4 1.3547238815331898E-5 2.9052663265225944 8.128343289199139E-5 60-61 3.3868097038329743E-4 1.4901962696865087E-4 1.3547238815331898E-5 3.2350332137654036 8.128343289199139E-5 62-63 3.5222820919862934E-4 1.4901962696865087E-4 1.3547238815331898E-5 3.5681869107120456 9.483067170732328E-5 64-65 3.5222820919862934E-4 1.4901962696865087E-4 1.3547238815331898E-5 3.908330982887399 9.483067170732328E-5 66-67 3.5222820919862934E-4 1.6256686578398278E-4 1.3547238815331898E-5 4.2741741670954365 9.483067170732328E-5 68-69 3.5222820919862934E-4 1.6256686578398278E-4 1.3547238815331898E-5 4.667355679232815 9.483067170732328E-5 70-71 3.5222820919862934E-4 1.6256686578398278E-4 1.3547238815331898E-5 5.0961528822157 9.483067170732328E-5 72-73 3.5222820919862934E-4 1.6256686578398278E-4 1.3547238815331898E-5 5.5328007100920695 9.483067170732328E-5 74-75 3.590018286062953E-4 1.6256686578398278E-4 1.3547238815331898E-5 5.966874562593526 9.483067170732328E-5 76-77 3.6577544801396126E-4 1.7611410459931467E-4 1.3547238815331898E-5 6.421479255519619 9.483067170732328E-5 78-79 3.6577544801396126E-4 1.7611410459931467E-4 1.3547238815331898E-5 6.905237606376306 9.483067170732328E-5 80-81 3.793226868292931E-4 2.0320858222997847E-4 1.3547238815331898E-5 7.424936781810068 9.483067170732328E-5 82-83 3.793226868292931E-4 2.0320858222997847E-4 1.3547238815331898E-5 7.975273037824704 1.0837791052265518E-4 84-85 3.793226868292931E-4 2.0320858222997847E-4 1.3547238815331898E-5 8.519052430252717 1.0837791052265518E-4 86-87 3.793226868292931E-4 2.0320858222997847E-4 1.3547238815331898E-5 9.071061770261046 1.0837791052265518E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 34230 0.0 36.225803 1 ATCAACG 43205 0.0 28.264114 3 CAACGCA 43435 0.0 28.018093 5 TATCAAC 44275 0.0 27.833124 2 GGTATCA 20985 0.0 27.449814 1 AACGCAG 46000 0.0 26.928904 6 TCAACGC 45460 0.0 26.903015 4 GTACTAG 2675 0.0 25.047619 1 ACGCAGA 49565 0.0 24.579243 7 CGCAGAG 50125 0.0 24.30464 8 GCAGAGT 54440 0.0 22.429617 9 AGTACTT 31520 0.0 22.197958 12-13 GAGTACT 29840 0.0 20.36966 12-13 AGAGTAC 51165 0.0 20.35965 10-11 TACTTTT 35495 0.0 19.856182 14-15 TCTAACG 1070 0.0 18.687849 2 TAGTACT 3405 0.0 18.573616 4 TAGGCAT 4055 0.0 18.463314 5 CTAACGC 1095 0.0 18.261187 3 GTACTTT 33530 0.0 18.17651 14-15 >>END_MODULE