FastQCFastQC Report
Fri 27 May 2016
ERR765491_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765491_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13167024
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA465130.35325370410200513No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT457070.3471323512435308No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT197210.14977568203718622No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG184580.14018353729741817No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA146540.11129318211920933No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT142860.10849832126074957No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG138080.10486804003698937No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC137890.10472374015571022No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG59750.022.9571251
GTATAAG65050.020.8700331
GTACAAG53000.020.8288141
TAGGCAT86300.020.5725235
GTACATA35300.020.0943871
TAGTACT68600.020.0625614
CATGGGG317650.019.430744
CTATTGA58750.019.1873469
GTGATCG50100.019.1252568
CTAGGCA97650.019.048724
TACATGA46550.018.466552
TCTAACG30800.017.8438912
CTAGTAC78800.017.7044723
CGTTAAC17900.017.5830551
CTAACGC31700.017.4853973
AGGGCTA69600.017.4782165
GGGCTAT69250.017.4305736
ATAAGGT62900.017.2509943
TTTAGGG69200.017.2417772
TGATCGC55100.017.0486499
Produced by FastQC (version 0.11.5)