FastQCFastQC Report
Fri 27 May 2016
ERR765489_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765489_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20834426
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA970550.46583956764635603No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT892600.42842552993780586No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG410420.19699126820196533No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC405240.19450499860183332No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT359550.1725749487890859No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT322310.1547006862584071No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC320050.1536159431510136No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC290420.139394289048328No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA279910.13434975362412194No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC279630.13421536067276343No Hit
GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG266780.12804768415506143No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC253470.12165921921727049No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC242300.11629790040771941No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG240790.11557313842003615No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA239680.11504036636286501No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT239010.11471878322925719No Hit
GTCCTACACTATCTTGGATATGATATGGCGCACTACACATGCTAGCCGCT237370.11393162451415748No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG234970.11277968493108473No Hit
GTGTAGGACTCGTCGCATTGGATGACGATGCCTAGTACTGTGCGCCAATT225180.10808073138180048No Hit
ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA223080.10707278424661185No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT217640.10446172119164694No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA217210.1042553320163464No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG134650.029.5991
TATTCGC25650.027.4851259
GTATAAG160400.023.9682581
GTGATCG128600.023.6456288
ACGTTAA26000.022.9571488
TAGTACT192800.022.914454
CTAACGC81100.022.7750993
TAGGCAT235400.022.4812555
TCTAACG82850.022.350762
GGCGTCG112800.022.1244748
CGTTAAC39800.022.082391
CTAGGCA253400.021.6262174
CTGTGCG201550.021.476859
CGCCTAC85150.021.4710127
TACGGGC115950.021.4424064
TGATCGC144100.021.1677429
ATACGGG118400.021.1574863
CGGGCGT117550.021.0305526
GTCCTAC142850.020.7933461
ATAAGGT153650.020.73893
Produced by FastQC (version 0.11.5)