FastQCFastQC Report
Fri 27 May 2016
ERR765488_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765488_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13237376
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1695031.2804879154297648No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1424081.0758023342390515No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG668430.5049565714534361No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG520560.3932501426264541No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT492590.3721205773712252No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC484890.3663037145730393No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG482990.36486838479166867No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC469180.3544358035912858No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC357770.2702725978320779No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC311670.2354469647156657No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA303500.22927504665577225No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT288200.21771686473210403No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG279290.21098592349420306No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG201210.15200142384714313No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC196200.14821668584468706No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA189560.14320058597716043No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC181090.13680203689915585No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT181020.13674915632826326No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC166580.12584064998984693No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC158300.11958563388997939No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA156820.11846758753396444No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG146830.11092077463086339No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA135860.10263363373526596No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG134500.10160623978649547No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG41750.020.5822731
TAGTACT54950.020.0808724
TACATGA53750.019.0440962
GTACAAG60550.018.534571
CATGGGG389550.018.08054
CGTTAAC11750.017.5838341
AATACGG29300.017.4679225
GTATAAG55700.017.113531
CTAGTAC62950.017.0813473
CTGTGCG65050.016.3859189
TAATACG31850.016.3642434
TAGGCAT67300.016.2563575
GTCCTAC42800.016.237411
GTATATA40200.016.00271
GTATAAA112350.015.7567641
CCTACAC53900.015.5064943
ATGGGGG151800.015.3732975
GTACATA37200.015.1473641
ATACGGG35250.015.0522593
GTATAAT34500.014.9717431
Produced by FastQC (version 0.11.5)