##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765488_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13237376
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.15980274338358	33.0	33.0	33.0	33.0	33.0
2	32.07247554198052	33.0	33.0	33.0	33.0	33.0
3	32.11136610458145	33.0	33.0	33.0	33.0	33.0
4	35.873648145976965	37.0	37.0	37.0	33.0	37.0
5	35.90850067264086	37.0	37.0	37.0	33.0	37.0
6	35.887934663184005	37.0	37.0	37.0	33.0	37.0
7	35.825317419403966	37.0	37.0	37.0	33.0	37.0
8	35.78281813555798	37.0	37.0	37.0	33.0	37.0
9	35.78937751711518	37.0	37.0	37.0	33.0	37.0
10-11	35.77626865022192	37.0	37.0	37.0	33.0	37.0
12-13	35.761666851496855	37.0	37.0	37.0	33.0	37.0
14-15	37.56883222173337	40.0	37.0	40.0	33.0	40.0
16-17	37.58602090021466	40.0	37.0	40.0	33.0	40.0
18-19	37.627864276122395	40.0	37.0	40.0	33.0	40.0
20-21	37.648981338899794	40.0	37.0	40.0	33.0	40.0
22-23	37.66906031829873	40.0	37.0	40.0	33.0	40.0
24-25	37.68360462828887	40.0	37.0	40.0	33.0	40.0
26-27	37.65849398702582	40.0	37.0	40.0	33.0	40.0
28-29	37.61140776691695	40.0	37.0	40.0	33.0	40.0
30-31	37.546021394270284	40.0	37.0	40.0	33.0	40.0
32-33	37.429460453491686	40.0	37.0	40.0	33.0	40.0
34-35	37.35725471573822	40.0	37.0	40.0	33.0	40.0
36-37	37.29331515551118	40.0	37.0	40.0	33.0	40.0
38-39	37.17949014215506	40.0	37.0	40.0	33.0	40.0
40-41	37.060899720609285	40.0	37.0	40.0	33.0	40.0
42-43	36.909782648766644	40.0	37.0	40.0	33.0	40.0
44-45	36.72604986063703	38.5	37.0	40.0	33.0	40.0
46-47	36.52659035295213	37.0	37.0	40.0	33.0	40.0
48-49	36.35805022838363	37.0	37.0	40.0	33.0	40.0
50-51	36.15782697416769	37.0	37.0	40.0	33.0	40.0
52-53	36.04122323789851	37.0	37.0	40.0	30.0	40.0
54-55	35.87438870060048	37.0	37.0	40.0	27.0	40.0
56-57	35.68390582091194	37.0	37.0	40.0	27.0	40.0
58-59	35.4697043809891	37.0	35.0	40.0	27.0	40.0
60-61	35.27016936740333	37.0	33.0	40.0	27.0	40.0
62-63	35.00153263003181	37.0	33.0	40.0	27.0	40.0
64-65	34.829127162362084	37.0	33.0	37.0	27.0	40.0
66-67	34.6523596519431	37.0	33.0	37.0	27.0	40.0
68-69	34.40351667883424	37.0	33.0	37.0	27.0	40.0
70-71	34.13482993155139	37.0	33.0	37.0	27.0	40.0
72-73	33.878159160848796	37.0	33.0	37.0	27.0	37.0
74-75	33.6420423881591	37.0	33.0	37.0	27.0	37.0
76-77	33.42219269891555	37.0	33.0	37.0	27.0	37.0
78-79	33.259049754271544	37.0	33.0	37.0	27.0	37.0
80-81	33.098977017801715	37.0	33.0	37.0	27.0	37.0
82-83	32.97220438552172	37.0	33.0	37.0	27.0	37.0
84-85	32.81832328401037	37.0	33.0	37.0	27.0	37.0
86-87	32.67785205315615	37.0	33.0	37.0	24.5	37.0
88-89	32.57819230941238	37.0	33.0	37.0	22.0	37.0
90-91	32.45237050756887	33.0	33.0	37.0	22.0	37.0
92-93	32.320179089874	33.0	33.0	37.0	22.0	37.0
94-95	32.25653592524682	33.0	33.0	37.0	22.0	37.0
96-97	32.19972096433614	33.0	33.0	37.0	22.0	37.0
98-99	32.09228955194746	33.0	33.0	37.0	22.0	37.0
100	31.973618865249428	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	47.0
5	81.0
6	390.0
7	955.0
8	1935.0
9	2955.0
10	3443.0
11	4863.0
12	7652.0
13	13088.0
14	22454.0
15	28820.0
16	34057.0
17	41769.0
18	47872.0
19	55254.0
20	63176.0
21	76003.0
22	89896.0
23	91257.0
24	88248.0
25	91425.0
26	102474.0
27	118739.0
28	145115.0
29	183594.0
30	236602.0
31	305228.0
32	408458.0
33	554206.0
34	768879.0
35	1140499.0
36	2073747.0
37	4114545.0
38	2316598.0
39	3049.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.975671411566076	18.671018099391937	12.64875621228884	24.704554276753147
2	15.201020342947762	21.833068195055752	36.879983996516465	26.085927465480026
3	18.81423688701016	27.267186469297588	29.117257163301485	24.801319480390763
4	13.29567128711914	18.191717149985013	35.971607968225726	32.54100359467012
5	13.695189702818308	38.069739261555846	33.48284583690287	14.752225198722977
6	31.08382383325357	35.44257553157374	18.674160112922948	14.799440522249741
7	26.63775660674744	31.945001788874173	22.759042275447943	18.658199328930447
8	27.65451400640127	32.07791332662908	21.068873468578666	19.19869919839098
9	27.952987057253644	14.823119022984615	19.640168867304215	37.58372505245753
10-11	24.765085618176897	26.16655672544166	27.112964079890155	21.955393576491293
12-13	26.70699615996403	23.769718409449123	26.608683624307417	22.914601806279432
14-15	25.230656321684087	24.097082229920147	24.61698295011798	26.05527849827779
16-17	23.699597774881155	26.573714654208935	26.661934176866335	23.064753394043574
18-19	24.46208750308711	26.084050359864957	27.723472524028836	21.7303896130191
20-21	24.615610882575403	25.566312036387835	26.953753622015064	22.864323459021698
22-23	23.706447868920836	25.852552564805876	25.460929081904833	24.980070484368458
24-25	23.694394223630294	26.494988938826324	25.432054544527126	24.378562293016255
26-27	24.529397175891873	25.36796438330865	25.41746811426226	24.68517032653722
28-29	24.020897713483116	25.187615672650253	26.796761679725595	23.994724934141036
30-31	25.00924700577645	25.292438919502302	26.2889124357301	23.409401638991152
32-33	23.123763743147467	25.912128814931144	26.38276904452524	24.581338397396152
34-35	23.07532131751566	26.21266682909263	26.9026635023459	23.809348351045813
36-37	24.600790835293086	25.615700789145972	26.035789285995435	23.747719089565503
38-39	25.1162968593149	25.078960167657204	25.737856967627966	24.066886005399933
40-41	23.878443741250514	25.985175087214763	26.07522675308745	24.061154418447277
42-43	25.409054435006468	25.269262190044746	25.562201616000163	23.75948175894862
44-45	25.221595725022606	25.24140842079248	25.598407275412345	23.938588578772567
46-47	24.012100327486067	25.46067370894219	27.01025067965526	23.516975283916484
48-49	24.943535194462093	25.756075214060694	26.20572678991752	23.094662801559696
50-51	23.58100016636982	26.238583234993268	25.944966768923205	24.23544982971371
52-53	23.271661692005495	26.473909630246744	24.620446552671655	25.6339821250761
54-55	23.857161251568836	26.447906281052912	24.548770612291914	25.146161855086334
56-57	24.631603513631088	25.079997472729822	24.731458649053163	25.556940364585934
58-59	24.109081117161573	25.093960130220843	26.08332459168438	24.713634160933204
60-61	24.532817059790784	25.547389344825717	25.504253603544335	24.415539991839164
62-63	22.996416614025378	25.68853729457287	25.844279188768354	25.470766902633397
64-65	22.759967957777597	25.875798642116177	26.00693507588453	25.357298324221695
66-67	24.470141237608605	25.39397060807743	25.345712923224372	24.790175231089588
68-69	24.80433216084518	24.69913618545081	25.173043261155335	25.323488392548672
70-71	23.550381629918835	25.39439185306988	25.49691433075752	25.55831218625377
72-73	24.894814535606294	24.814414320193112	25.47762258236847	24.813148561832126
74-75	25.1315499806039	24.511120467187116	25.592268707107436	24.765060845101548
76-77	23.847889174859194	25.092333626021684	26.55818756733054	24.501589631788583
78-79	24.314093403626494	25.13102503753586	26.179077967955443	24.375803590882203
80-81	23.297947350521184	25.242707221330235	26.363721584658563	25.095623843490017
82-83	23.330607915514012	25.588499839965763	25.15294301300191	25.927949231518312
84-85	23.58918397619049	25.782838295649984	24.7550013666557	25.87297636150382
86-87	23.76259746815165	24.607709344474493	25.38099005501817	26.24870313235569
88-89	23.764956690643064	24.31899516382846	26.03882969356355	25.87721845196493
90-91	24.300270040822532	24.74858004437726	25.618028603389458	25.33312131141075
92-93	23.139162946748584	25.049982454244184	25.91425589921026	25.896598699796975
94-95	22.44814540340436	25.352935160962474	26.38575257103139	25.813166864601772
96-97	23.911096876601878	24.86733630059385	25.568166771631063	25.653400051173215
98-99	24.012685161138002	24.587249675363005	25.335133986542317	26.064931176956673
100	23.387680260235484	25.060470465776515	25.732182375464685	25.81966689852332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	309.0
1	274.0
2	404.5
3	619.0
4	557.0
5	501.0
6	744.0
7	1344.5
8	2134.5
9	2277.0
10	1669.5
11	1327.0
12	1450.0
13	1629.5
14	1901.5
15	2291.0
16	2723.0
17	3263.5
18	3936.0
19	4773.0
20	5930.0
21	7595.5
22	9837.0
23	12890.0
24	17100.5
25	23073.0
26	31812.0
27	41662.5
28	51832.5
29	67292.5
30	85327.5
31	102633.0
32	121514.0
33	140320.5
34	162634.5
35	179317.5
36	192670.0
37	213689.5
38	229773.0
39	242173.0
40	251854.5
41	259924.5
42	273912.5
43	302868.0
44	343298.5
45	383170.5
46	434393.0
47	502256.5
48	615994.0
49	755799.5
50	992114.5
51	972973.5
52	679351.5
53	560427.0
54	517454.0
55	472140.0
56	437875.5
57	400160.0
58	372543.5
59	345271.0
60	292150.0
61	232247.5
62	188671.0
63	148484.5
64	107592.0
65	83313.5
66	65416.0
67	47526.0
68	36108.0
69	30144.5
70	26958.5
71	24099.0
72	21335.5
73	22999.0
74	18366.5
75	12790.5
76	9660.5
77	6843.0
78	5040.0
79	2926.0
80	1962.5
81	1525.5
82	1242.5
83	1021.5
84	758.0
85	487.0
86	327.5
87	197.5
88	95.5
89	57.0
90	40.0
91	39.5
92	29.0
93	19.5
94	14.5
95	13.0
96	8.0
97	4.0
98	6.0
99	6.0
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.028155126816674238
2	0.0019490267557558236
3	3.32392159896342E-4
4	0.0
5	0.0026893547482522216
6	2.3418538538151368E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	3.097290580852278E-4
16-17	0.0038527273078894186
18-19	0.006670506299737954
20-21	0.018980347766808167
22-23	0.02444593248692188
24-25	0.03135817853931172
26-27	0.030444100099596777
28-29	0.045870118065695194
30-31	0.045824791862072965
32-33	0.05604585077888548
34-35	0.05085977764777551
36-37	0.05429701475579451
38-39	0.058640775936258056
40-41	0.04175676508697796
42-43	0.0476907205778547
44-45	0.045031583298683966
46-47	0.042818153688465144
48-49	0.03623829979597165
50-51	0.03631762065231055
52-53	0.0376509664755311
54-55	0.05617805220611698
56-57	0.04312032837928
58-59	0.043059893441117034
60-61	0.04448766885521723
62-63	0.02978309296343928
64-65	0.031815217759169186
66-67	0.03210983808271367
68-69	0.03006638173607821
70-71	0.04263307169034105
72-73	0.03174722845373585
74-75	0.04028744065289072
76-77	0.04540174729493217
78-79	0.040514071671001864
80-81	0.033280765009621245
82-83	0.020457226568165773
84-85	0.032808617055223026
86-87	0.026938873686144442
88-89	0.026119224837309146
90-91	0.021873670431360417
92-93	0.014968978746240945
94-95	0.01493876127715946
96-97	0.01462147785180386
98-99	0.01086695731842927
100	0.005658221085508185
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3237376E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.125522305298592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.37972295502173	22.217311589825623
2	14.712666775098377	9.158788757575
3	5.388746880930127	5.031826837199967
4	2.4730856572981406	3.079043311565892
5	1.4785938054709786	2.301100223633164
6	0.8813776967716997	1.6460004696156143
7	0.578736569253952	1.2609434596644107
8	0.4327394288747001	1.0775392596657323
9	0.3212949069455478	0.9000424613441236
>10	1.9317025948611146	11.357841042074227
>50	0.1965683237606502	4.305039435115534
>100	0.1786043639370466	11.612534934637159
>500	0.026492477865924076	5.77810250806084
>1k	0.01795946407876238	10.354046573326674
>5k	9.028527681365264E-4	1.8374224447698138
>10k+	8.052470634731181E-4	8.082416691926413
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	169503	1.2804879154297648	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	142408	1.0758023342390515	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	66843	0.5049565714534361	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	52056	0.3932501426264541	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	49259	0.3721205773712252	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	48489	0.3663037145730393	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	48299	0.36486838479166867	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	46918	0.3544358035912858	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	35777	0.2702725978320779	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	31167	0.2354469647156657	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	30350	0.22927504665577225	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	28820	0.21771686473210403	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	27929	0.21098592349420306	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	20121	0.15200142384714313	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	19620	0.14821668584468706	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	18956	0.14320058597716043	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	18109	0.13680203689915585	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	18102	0.13674915632826326	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	16658	0.12584064998984693	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	15830	0.11958563388997939	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	15682	0.11846758753396444	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	14683	0.11092077463086339	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	13586	0.10263363373526596	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	13450	0.10160623978649547	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.554367270371409E-6	0.0	0.0	0.0	0.0
2	7.55436727037141E-5	0.0	0.0	0.0	0.0
3	7.55436727037141E-5	0.0	0.0	0.0	0.0
4	8.30980399740855E-5	0.0	0.0	0.0	0.0
5	8.30980399740855E-5	0.0	0.0	0.0	0.0
6	1.0576114178519973E-4	0.0	0.0	0.0	7.554367270371409E-6
7	1.2842424359631394E-4	0.0	0.0	0.0	7.554367270371409E-6
8	1.2842424359631394E-4	0.0	0.0	0.0	7.554367270371409E-6
9	1.2842424359631394E-4	0.0	0.0	0.0	7.554367270371409E-6
10-11	1.3975579450187106E-4	0.0	0.0	1.5108734540742818E-5	7.554367270371409E-6
12-13	1.510873454074282E-4	0.0	0.0	8.30980399740855E-5	1.5108734540742818E-5
14-15	1.813048144889138E-4	0.0	0.0	1.586417126777996E-4	2.2663101811114226E-5
16-17	1.9641354902965664E-4	0.0	0.0	2.568484871926279E-4	3.0217469081485635E-5
18-19	2.0774509993521376E-4	0.0	0.0	3.9660428169449894E-4	4.532620362222846E-5
20-21	2.1152228357039946E-4	0.0	0.0	7.327736252260267E-4	5.2880570892599865E-5
22-23	2.2663101811114228E-4	0.0	0.0	0.0017261729212798668	5.2880570892599865E-5
24-25	2.8706595627411353E-4	0.0	0.0	0.003486340495276405	5.2880570892599865E-5
26-27	3.0595187445004205E-4	0.0	0.0	0.005503356556465571	5.2880570892599865E-5
28-29	3.286149762611563E-4	0.0	0.0	0.015595991229681774	5.2880570892599865E-5
30-31	3.890499144241276E-4	0.0	0.0	0.04714302895075278	5.2880570892599865E-5
32-33	4.305989344111703E-4	0.0	0.0	0.09603111674096135	6.798930543334268E-5
34-35	4.5326203622228457E-4	0.0	0.0	0.15697975187831786	6.798930543334268E-5
36-37	4.7214795439821304E-4	0.0	0.0	0.25245562262490695	6.798930543334268E-5
38-39	4.7592513803339877E-4	0.0	0.0	0.3976958877650676	6.798930543334268E-5
40-41	4.948110562093273E-4	0.0	0.0	0.5970669715810747	6.798930543334268E-5
42-43	5.590231780074843E-4	0.0	0.0	0.7557237929934151	7.55436727037141E-5
44-45	5.892406470889699E-4	0.0	0.0	0.9286243738940406	8.30980399740855E-5
46-47	5.967950143593413E-4	0.0	0.0	1.1170378479843739	9.06524072444569E-5
48-49	6.345668507111984E-4	0.0	0.0	1.311691229440034	9.442959087964262E-5
50-51	6.458984016167555E-4	0.0	0.0	1.5137554451879285	9.820677451482832E-5
52-53	6.572299525223126E-4	0.0	0.0	1.731502527389114	1.0198395815001402E-4
54-55	6.761158706982411E-4	0.0	0.0	1.93339299268979	1.0576114178519973E-4
56-57	6.836702379686125E-4	0.0	0.0	2.154569757631724	1.0576114178519973E-4
58-59	6.874474216037982E-4	0.0	0.0	2.396796766972548	1.0953832542038543E-4
60-61	7.629910943075123E-4	0.0	0.0	2.630419351992419	1.1331550905557113E-4
62-63	7.856541961186265E-4	0.0	0.0	2.8673092008567256	1.2086987632594254E-4
64-65	7.969857470241837E-4	0.0	0.0	3.1243238841293017	1.2086987632594254E-4
66-67	8.120944815649265E-4	0.0	0.0	3.39643974757535	1.2086987632594254E-4
68-69	8.272032161056693E-4	0.0	0.0	3.7197968842163283	1.2086987632594254E-4
70-71	8.347575833760406E-4	0.0	0.0	4.11503382543489	1.2086987632594254E-4
72-73	8.649750524575263E-4	0.0	0.0	4.451482680555421	1.2464705996112824E-4
74-75	8.763066033630834E-4	0.0	0.0	4.780437603343744	1.2842424359631394E-4
76-77	8.763066033630834E-4	0.0	0.0	5.145876342864327	1.3597861086668537E-4
78-79	8.95192521539012E-4	0.0	0.0	5.50699776148989	1.3597861086668537E-4
80-81	8.989697051741977E-4	0.0	0.0	5.902389567237495	1.3597861086668537E-4
82-83	9.06524072444569E-4	0.0	0.0	6.35597266406877	1.3597861086668537E-4
84-85	9.216328069853118E-4	0.0	0.0	6.780675414825415	1.3975579450187106E-4
86-87	9.59404643337169E-4	1.5108734540742818E-5	0.0	7.2137295185994565	1.510873454074282E-4
88	9.745133778779118E-4	1.5108734540742818E-5	0.0	7.569944375682915	1.586417126777996E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	4175	0.0	20.582273	1
TAGTACT	5495	0.0	20.080872	4
TACATGA	5375	0.0	19.044096	2
GTACAAG	6055	0.0	18.53457	1
CATGGGG	38955	0.0	18.0805	4
CGTTAAC	1175	0.0	17.583834	1
AATACGG	2930	0.0	17.467922	5
GTATAAG	5570	0.0	17.11353	1
CTAGTAC	6295	0.0	17.081347	3
CTGTGCG	6505	0.0	16.385918	9
TAATACG	3185	0.0	16.364243	4
TAGGCAT	6730	0.0	16.256357	5
GTCCTAC	4280	0.0	16.23741	1
GTATATA	4020	0.0	16.0027	1
GTATAAA	11235	0.0	15.756764	1
CCTACAC	5390	0.0	15.506494	3
ATGGGGG	15180	0.0	15.373297	5
GTACATA	3720	0.0	15.147364	1
ATACGGG	3525	0.0	15.052259	3
GTATAAT	3450	0.0	14.971743	1
>>END_MODULE