FastQCFastQC Report
Fri 27 May 2016
ERR765487_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765487_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7764003
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA208830.26897207535854895No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT196910.2536191704202072No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG78610.10124931687944994No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA19100.024.8326281
GTACTAG32750.022.717711
GTACAAG36950.020.3871461
TAGTACT39200.020.0457784
GTACAAA40100.019.945271
GTATAAG36900.019.4066331
CTATTGA40050.018.807549
GGGCTAT44050.018.6821696
ATTTAGG43750.018.174981
ACGTTAA8750.018.066258
TAGGCAT48050.017.9987285
GTGATCG28850.017.8885158
CTAGGCA53150.017.846344
TTTAGGG46200.017.8150542
CTAGTAC45200.017.7976863
CTAACGC16550.017.7010253
CATGGGG197300.017.6277474
TGATCGC29450.017.3670839
GGCTATT45050.017.3386147
CGTTAAC10750.017.3024431
Produced by FastQC (version 0.11.5)