FastQCFastQC Report
Fri 27 May 2016
ERR765487_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765487_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7764003
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT220260.28369386256033136No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT204280.2631116963762121No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA193340.24902102691098907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137330.17688040563611324No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106430.1370813483714522No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA539300.025.5117571
AGTACTT336950.021.71163212-13
TATCACG7500.021.7015742
GTACTAG32300.021.6076811
CAACGCA642800.021.1525925
ATCAACG646350.021.0292193
AACGCAG670700.020.6396066
TATCAAC670900.020.5380082
TCAACGC683200.019.9766354
TAGTACT42750.019.6881314
GTATAAG33550.019.2771021
ACGCAGA706950.018.9761457
CGCAGAG709850.018.9260438
TACTTTT393150.018.88538614-15
GAGTACT323050.018.67956712-13
TAGGCAT47900.018.2509065
TCTAACG15350.018.1772142
GCAGAGT742350.017.9916639
TCACGCA9800.017.556494
ATTTAGG42400.017.4482041
Produced by FastQC (version 0.11.5)