##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765487_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7764003 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.303105112143825 33.0 33.0 33.0 33.0 33.0 2 32.14617459575943 33.0 33.0 33.0 33.0 33.0 3 32.223154086880186 33.0 33.0 33.0 33.0 33.0 4 36.14365772398594 37.0 37.0 37.0 33.0 37.0 5 36.0901605782481 37.0 37.0 37.0 33.0 37.0 6 36.09089344761974 37.0 37.0 37.0 33.0 37.0 7 36.004746262978 37.0 37.0 37.0 33.0 37.0 8 35.96858102708101 37.0 37.0 37.0 33.0 37.0 9 35.71729261310177 37.0 37.0 37.0 33.0 37.0 10-11 35.893287071115246 37.0 37.0 37.0 33.0 37.0 12-13 35.8174706398233 37.0 37.0 37.0 33.0 37.0 14-15 37.211272329492914 37.0 37.0 40.0 33.0 40.0 16-17 37.358254820354915 37.0 37.0 40.0 33.0 40.0 18-19 37.42615008005535 38.5 37.0 40.0 33.0 40.0 20-21 37.450869738200765 37.0 37.0 40.0 33.0 40.0 22-23 37.37380710697819 37.0 37.0 40.0 33.0 40.0 24-25 37.318821940176996 37.0 37.0 40.0 33.0 40.0 26-27 37.182893347671296 37.0 37.0 40.0 33.0 40.0 28-29 37.12877905894678 37.0 37.0 40.0 33.0 40.0 30-31 37.0244883985748 37.0 37.0 40.0 33.0 40.0 32-33 36.844547780313846 37.0 37.0 40.0 33.0 40.0 34-35 36.75959521138773 37.0 37.0 40.0 33.0 40.0 36-37 36.60485956793165 37.0 37.0 40.0 33.0 40.0 38-39 36.456456418164706 37.0 37.0 40.0 33.0 40.0 40-41 36.3165694294554 37.0 37.0 40.0 33.0 40.0 42-43 36.13940566483552 37.0 37.0 40.0 33.0 40.0 44-45 35.91760873868802 37.0 37.0 40.0 30.0 40.0 46-47 35.66352209034437 37.0 37.0 40.0 27.0 40.0 48-49 35.41015607541625 37.0 37.0 40.0 27.0 40.0 50-51 35.10610274107313 37.0 33.0 40.0 27.0 40.0 52-53 34.94918304384993 37.0 33.0 40.0 27.0 40.0 54-55 34.828922979550626 37.0 33.0 40.0 27.0 40.0 56-57 34.64977151605943 37.0 33.0 40.0 27.0 40.0 58-59 34.45463699588988 37.0 33.0 38.5 27.0 40.0 60-61 34.27971318403664 37.0 33.0 37.0 27.0 40.0 62-63 33.96628665650954 37.0 33.0 37.0 27.0 40.0 64-65 33.81749124774939 37.0 33.0 37.0 27.0 40.0 66-67 33.62726798276611 37.0 33.0 37.0 27.0 40.0 68-69 33.38735739798143 37.0 33.0 37.0 27.0 40.0 70-71 33.192604639642724 37.0 33.0 37.0 27.0 37.0 72-73 32.98452718269171 37.0 33.0 37.0 27.0 37.0 74-75 32.777305985069816 37.0 33.0 37.0 22.0 37.0 76-77 32.61281551539844 35.0 33.0 37.0 22.0 37.0 78-79 32.423527077462495 33.0 33.0 37.0 22.0 37.0 80-81 32.290185423679 33.0 33.0 37.0 22.0 37.0 82-83 32.1507659772929 33.0 33.0 37.0 22.0 37.0 84-85 31.998386785785634 33.0 33.0 37.0 22.0 37.0 86-87 31.90634483268489 33.0 33.0 37.0 22.0 37.0 88-89 31.804585147635827 33.0 33.0 37.0 22.0 37.0 90-91 31.742966675824313 33.0 33.0 37.0 22.0 37.0 92-93 31.644942563777988 33.0 33.0 37.0 22.0 37.0 94-95 31.58069316820202 33.0 33.0 37.0 22.0 37.0 96-97 31.534035149136344 33.0 33.0 37.0 22.0 37.0 98-99 30.699222875107083 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.17619114787414958 1101 2 0.3557692964632295 1101 3 0.24339796736160935 1101 4 0.2385244937696669 1101 5 0.07917726056250984 1101 6 0.16839593671798525 1101 7 0.22361101465948963 1101 8 0.15090496856846158 1101 9 0.4605050075639312 1101 10-11 0.3816469834500822 1101 12-13 0.3538020294986026 1101 14-15 0.6441819081114488 1101 16-17 0.5112045267662708 1101 18-19 0.37129667000547784 1101 20-21 0.42894011073648386 1101 22-23 0.3480165493284417 1101 24-25 0.4165914115072553 1101 26-27 0.29996573749242117 1101 28-29 0.3039832969334242 1101 30-31 0.3823107373576846 1101 32-33 0.41739549044977053 1101 34-35 0.4703444128156846 1101 36-37 0.4840541546475947 1101 38-39 0.3092491136798188 1101 40-41 0.3256681043546479 1101 42-43 0.4422124170216506 1101 44-45 0.4470231100536637 1101 46-47 0.4981206071770288 1101 48-49 0.6028772686666954 1101 50-51 0.5840270473433335 1101 52-53 0.5762844858729821 1101 54-55 0.5459137835380687 1101 56-57 0.5946915760576417 1101 58-59 0.597070970281564 1101 60-61 0.487688285039674 1101 62-63 0.5109703792532798 1101 64-65 0.4845079662497582 1101 66-67 0.5202821660232075 1101 68-69 0.4064470049472746 1101 70-71 0.39778606613193546 1101 72-73 0.4711225043811922 1101 74-75 0.30793564927896 1101 76-77 0.3514280746368712 1101 78-79 0.5948954126029236 1101 80-81 0.500498639432621 1101 82-83 0.4288171942430026 1101 84-85 0.5665421888066717 1101 86-87 0.39675954810492087 1101 88-89 0.4971529170892701 1101 90-91 0.4012163820601913 1101 92-93 0.5477743795272758 1101 94-95 0.6388105900513672 1101 96-97 0.4573876189612278 1101 98-99 0.500905381268133 1102 1 0.10632518938317048 1102 2 0.3669197252777252 1102 3 0.21269462723295618 1102 4 0.21210178000660562 1102 5 0.10546127857679011 1102 6 0.19427818937003138 1102 7 0.1751510568442214 1102 8 0.13876182901176293 1102 9 0.38226480795769646 1102 10-11 0.3622378721982571 1102 12-13 0.33019788414027573 1102 14-15 0.5792959936174213 1102 16-17 0.436801139052001 1102 18-19 0.3334114936622541 1102 20-21 0.4133084746631539 1102 22-23 0.26462957350977234 1102 24-25 0.36729008025757537 1102 26-27 0.31692543025596365 1102 28-29 0.31516958634487935 1102 30-31 0.28804127192759665 1102 32-33 0.38852847368929844 1102 34-35 0.42151273057253746 1102 36-37 0.4057291079375389 1102 38-39 0.21150450118961572 1102 40-41 0.26573910796403055 1102 42-43 0.21554338100963832 1102 44-45 0.36825836585280314 1102 46-47 0.47297746657710604 1102 48-49 0.5047410770866847 1102 50-51 0.38087982448484325 1102 52-53 0.459934855447635 1102 54-55 0.34479685541380434 1102 56-57 0.4852254254060213 1102 58-59 0.3751111553125597 1102 60-61 0.2965437623257756 1102 62-63 0.4097208954595857 1102 64-65 0.2980464202105608 1102 66-67 0.36636259718792274 1102 68-69 0.38529999415995775 1102 70-71 0.36071998680658623 1102 72-73 0.4264104539836566 1102 74-75 0.3573318984058318 1102 76-77 0.27341737912533404 1102 78-79 0.5161721698243298 1102 80-81 0.40658707580031006 1102 82-83 0.41432849966411567 1102 84-85 0.43593606908496696 1102 86-87 0.3519814024357153 1102 88-89 0.402962527535518 1102 90-91 0.3604598579650755 1102 92-93 0.4389790660769748 1102 94-95 0.4682826132715938 1102 96-97 0.3673412874425459 1102 98-99 0.3230766377683061 1103 1 0.09166737455335294 1103 2 0.23637094261676594 1103 3 0.23966904444422 1103 4 0.17929935411716258 1103 5 0.09322939050566248 1103 6 0.18190213769504737 1103 7 0.18085188209968805 1103 8 0.10017136317821951 1103 9 0.4100271802121611 1103 10-11 0.27434599154243244 1103 12-13 0.2667262096014582 1103 14-15 0.48291902314669954 1103 16-17 0.4308605387111655 1103 18-19 0.1812259857430476 1103 20-21 0.2810093152194426 1103 22-23 0.18229685488388014 1103 24-25 0.3030330070325036 1103 26-27 0.14852539808691034 1103 28-29 0.07333752929295656 1103 30-31 0.09882394818701101 1103 32-33 0.1777130872567554 1103 34-35 0.2789420356409025 1103 36-37 0.24803028447750108 1103 38-39 0.02369565548553254 1103 40-41 -0.017031102230092188 1103 42-43 0.033410991202153184 1103 44-45 0.173289854759048 1103 46-47 0.2613006065148795 1103 48-49 0.22675528483340912 1103 50-51 0.17739963373209378 1103 52-53 0.25678182240476133 1103 54-55 0.1685585290550975 1103 56-57 0.2688420136621943 1103 58-59 0.2792584432697467 1103 60-61 0.1424716070052625 1103 62-63 0.23702538126891426 1103 64-65 0.016470569834687865 1103 66-67 0.12285110692326384 1103 68-69 0.1454266289922046 1103 70-71 0.12413446985615906 1103 72-73 0.18351047415462318 1103 74-75 0.12144878250899893 1103 76-77 0.06556568738616164 1103 78-79 0.35308253070169826 1103 80-81 0.15788179595293172 1103 82-83 0.22889997680365326 1103 84-85 0.23141980738939338 1103 86-87 0.2477886225206909 1103 88-89 0.20495697109777566 1103 90-91 0.05026540503572363 1103 92-93 -0.002463137211620392 1103 94-95 0.20283485615759744 1103 96-97 0.16910373694749836 1103 98-99 0.11710888779718331 1104 1 0.09557548688706419 1104 2 0.33210032620007723 1104 3 0.21406359556070242 1104 4 0.2264529402846165 1104 5 0.06665307526889563 1104 6 0.13835989962360884 1104 7 0.11956521566417422 1104 8 0.06444860363889404 1104 9 0.36120388295329064 1104 10-11 0.273208173220425 1104 12-13 0.26382890812271853 1104 14-15 0.4172191223748456 1104 16-17 0.3135024552463008 1104 18-19 0.24976872624947077 1104 20-21 0.31293650050061217 1104 22-23 0.21064142840564415 1104 24-25 0.3002388501195128 1104 26-27 0.1511493491664595 1104 28-29 0.19599233392967363 1104 30-31 0.20848117562780288 1104 32-33 0.2507084066239429 1104 34-35 0.3291931463903026 1104 36-37 0.23179701491843474 1104 38-39 0.20455120767189072 1104 40-41 0.14871932546539313 1104 42-43 0.18543426561035403 1104 44-45 0.21178950806050523 1104 46-47 0.2846265759549098 1104 48-49 0.38565600345899753 1104 50-51 0.3189355035044841 1104 52-53 0.3010734247763196 1104 54-55 0.2539951215020011 1104 56-57 0.30253176664601256 1104 58-59 0.27469434592677544 1104 60-61 0.2774744784304204 1104 62-63 0.2989942587157799 1104 64-65 0.19052007878455157 1104 66-67 0.24578248637710942 1104 68-69 0.17495934899412902 1104 70-71 0.2351996268768488 1104 72-73 0.2749579963477089 1104 74-75 0.1993563222508712 1104 76-77 0.16842671784387875 1104 78-79 0.3964954902263429 1104 80-81 0.3244955909993479 1104 82-83 0.24277464353098566 1104 84-85 0.3003293475218811 1104 86-87 0.29928692782404553 1104 88-89 0.21647014553208876 1104 90-91 0.16630564017676974 1104 92-93 0.21675328579431863 1104 94-95 0.2859099452689513 1104 96-97 0.2621661440844463 1104 98-99 0.19711061337610047 1105 1 0.12149987847924137 1105 2 0.29675857938180883 1105 3 0.25215063674239246 1105 4 0.1871465686716718 1105 5 -0.062413416809633304 1105 6 0.09066527714356454 1105 7 0.12675671016905454 1105 8 0.008630637710894007 1105 9 0.28186287437896596 1105 10-11 0.17392012053639405 1105 12-13 0.1841113008118498 1105 14-15 0.33850178356598803 1105 16-17 0.27781898447138786 1105 18-19 0.16989224494122368 1105 20-21 0.23612674241785214 1105 22-23 0.12365269188837402 1105 24-25 0.23200557756938167 1105 26-27 0.06033983643651197 1105 28-29 0.13266928804533507 1105 30-31 0.12746288496679625 1105 32-33 0.18574398867675512 1105 34-35 0.2707724046800166 1105 36-37 0.16783978147785916 1105 38-39 0.11241442595252948 1105 40-41 0.05592028238737612 1105 42-43 0.11571594283089581 1105 44-45 0.11995053151186141 1105 46-47 0.22049780834638 1105 48-49 0.21255993998267542 1105 50-51 0.14422471830732775 1105 52-53 0.027972811978045797 1105 54-55 0.15455438471659022 1105 56-57 0.13706914618423838 1105 58-59 0.14024458540888673 1105 60-61 0.13960553726381875 1105 62-63 0.13247582603943897 1105 64-65 -0.02723254726264912 1105 66-67 0.04882229613608047 1105 68-69 -0.015643379512020772 1105 70-71 0.07060116190778842 1105 72-73 0.07599642077776281 1105 74-75 0.045967090346366035 1105 76-77 0.029283657398835317 1105 78-79 0.12702448578408365 1105 80-81 0.1472987897190592 1105 82-83 0.052250859563734764 1105 84-85 0.018475589127739056 1105 86-87 0.08129190372399364 1105 88-89 0.009290264451131236 1105 90-91 0.01941745977815401 1105 92-93 -0.006584711312918046 1105 94-95 0.23587618912761954 1105 96-97 0.07760618472582337 1105 98-99 0.016429842431097796 1106 1 0.09509763325612397 1106 2 0.250759101002231 1106 3 0.19919747551744393 1106 4 0.1412737981805492 1106 5 -0.03922588744252664 1106 6 0.10616270205333933 1106 7 0.11256065583037866 1106 8 0.00473625252201515 1106 9 0.3078891339756069 1106 10-11 0.19476684946713618 1106 12-13 0.18323352464457798 1106 14-15 0.3250025586235239 1106 16-17 0.2620716490307089 1106 18-19 0.18345583700754275 1106 20-21 0.2589292030920092 1106 22-23 0.077883784751414 1106 24-25 0.21067299168058184 1106 26-27 0.07941749330597503 1106 28-29 0.12396973317460436 1106 30-31 0.03268570435432849 1106 32-33 0.044335514425306144 1106 34-35 0.13474263798876507 1106 36-37 0.053683510614803254 1106 38-39 -0.03486025167966744 1106 40-41 -0.03635390372827629 1106 42-43 -0.01242475580971103 1106 44-45 0.028153222383274112 1106 46-47 0.18652476145558694 1106 48-49 0.2552258263185365 1106 50-51 0.09208742619703258 1106 52-53 0.14445380393905793 1106 54-55 0.14883718984111027 1106 56-57 0.22165306075680036 1106 58-59 0.09623260284275403 1106 60-61 0.11606802010393835 1106 62-63 0.1245856452687093 1106 64-65 -0.010788282885307865 1106 66-67 0.07915479310681661 1106 68-69 0.05095897857005838 1106 70-71 0.05125667618519003 1106 72-73 0.13130501844089082 1106 74-75 0.10545188288990914 1106 76-77 0.014132338204120742 1106 78-79 0.14379538860761443 1106 80-81 0.14993178049434164 1106 82-83 0.06460659576088545 1106 84-85 0.06597578561348882 1106 86-87 0.06762743096212276 1106 88-89 -0.09367744958825241 1106 90-91 -0.12312293875082503 1106 92-93 -0.04926521232050618 1106 94-95 0.08187084548318424 1106 96-97 0.06515941522808433 1106 98-99 -0.0205756546336211 1107 1 0.08069684447181658 1107 2 0.2340203970393233 1107 3 0.1658749662006258 1107 4 0.12389595474282089 1107 5 0.0010810707332851166 1107 6 0.12816523021246695 1107 7 0.11719264209940405 1107 8 0.028393030169333144 1107 9 0.3162408585315504 1107 10-11 0.1947558693037621 1107 12-13 0.1636321163382206 1107 14-15 0.2749299927945401 1107 16-17 0.17779867241066682 1107 18-19 0.09978125666973625 1107 20-21 0.22755916171638546 1107 22-23 0.09495585761107606 1107 24-25 0.16415626910405479 1107 26-27 0.03594664716755602 1107 28-29 -0.013201469922485387 1107 30-31 0.02540338854675639 1107 32-33 -0.05847760899913368 1107 34-35 0.13266771425409019 1107 36-37 0.14244808750382987 1107 38-39 0.09813186295516374 1107 40-41 0.07311069640597623 1107 42-43 -0.019189580519807237 1107 44-45 -0.0035717131856145556 1107 46-47 0.03302453455773957 1107 48-49 0.13806054353064212 1107 50-51 -0.01666459439664436 1107 52-53 0.016479147172354658 1107 54-55 0.03474767528948064 1107 56-57 0.10851541674195175 1107 58-59 0.06771330421897659 1107 60-61 0.11247948693877419 1107 62-63 0.12033842062942313 1107 64-65 0.005339941395291703 1107 66-67 -0.03827509595727463 1107 68-69 -0.02811269988075793 1107 70-71 0.007582058399975722 1107 72-73 0.1078757650917197 1107 74-75 0.020285194056427258 1107 76-77 0.049428482293713216 1107 78-79 0.11503591500618171 1107 80-81 0.004937049163032725 1107 82-83 -0.0434607081093219 1107 84-85 -0.039475831046601684 1107 86-87 0.003232587127122599 1107 88-89 -0.06150442590698901 1107 90-91 -0.06733035164948831 1107 92-93 -0.11776287163505117 1107 94-95 0.11565680122012978 1107 96-97 0.01741559065990117 1107 98-99 -0.03931391073114909 1108 1 0.09239420409100774 1108 2 0.24039158905608105 1108 3 0.15397107238294439 1108 4 0.114318483267418 1108 5 -0.066358477421403 1108 6 0.043149642924099396 1108 7 0.0882656169155922 1108 8 0.0067282813998446045 1108 9 0.28158517624551394 1108 10-11 0.17646053000003548 1108 12-13 0.13595641430144667 1108 14-15 0.18887439071154688 1108 16-17 0.20480915291728508 1108 18-19 -0.010037046471339295 1108 20-21 0.18149156234498776 1108 22-23 0.07073481388862746 1108 24-25 0.06982544755126696 1108 26-27 -0.08626671774977268 1108 28-29 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2215 90-91 -0.2904697721396019 2215 92-93 -0.1442987365139743 2215 94-95 -0.18402433941531982 2215 96-97 -0.21541105674991812 2215 98-99 -0.060100033945008846 2216 1 -0.23962533344513304 2216 2 0.01608665244166474 2216 3 -0.12329212570971748 2216 4 -0.30571926659601445 2216 5 -0.24448462753725408 2216 6 -0.23519752859986198 2216 7 -0.2782365944541354 2216 8 -0.18116029301612713 2216 9 0.1328037802342763 2216 10-11 -0.17732146153885253 2216 12-13 -0.13952328973753936 2216 14-15 -0.43239125811754775 2216 16-17 -0.5530813087705653 2216 18-19 -0.573086511091482 2216 20-21 -0.8328704063691106 2216 22-23 -0.7547981071356631 2216 24-25 -0.584165788848722 2216 26-27 -0.6782744459135799 2216 28-29 -0.742206195661069 2216 30-31 -0.42653243200422963 2216 32-33 -0.45639789320157576 2216 34-35 -0.4678940323135876 2216 36-37 -0.43022933842076583 2216 38-39 -0.465283135610008 2216 40-41 -0.43632373477410624 2216 42-43 -0.48571600653215086 2216 44-45 -0.346127494055537 2216 46-47 -0.6597868569594709 2216 48-49 -0.907576461286105 2216 50-51 -0.46600837604287193 2216 52-53 -0.4405463436332866 2216 54-55 -0.4013880410488113 2216 56-57 -0.4637973981392989 2216 58-59 -0.31499044119293984 2216 60-61 -0.3827388191987282 2216 62-63 -0.42489240812736284 2216 64-65 -0.4582195823765858 2216 66-67 -0.4812472878865037 2216 68-69 -0.42206635981572305 2216 70-71 -0.4181629349239273 2216 72-73 -0.28661930234513733 2216 74-75 -0.39981079852753965 2216 76-77 -0.3263893912415199 2216 78-79 -0.24518000765861814 2216 80-81 -0.1774680556188457 2216 82-83 -0.16555048992868038 2216 84-85 -0.2778214450615799 2216 86-87 -0.38978361801675376 2216 88-89 -0.2438809107353741 2216 90-91 -0.25722857281994393 2216 92-93 -0.2573753774751566 2216 94-95 -0.3103058865623396 2216 96-97 -0.28656807711720944 2216 98-99 -0.29648630329898396 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 5.0 9 31.0 10 107.0 11 197.0 12 282.0 13 616.0 14 1756.0 15 4546.0 16 9930.0 17 18348.0 18 28869.0 19 39647.0 20 51024.0 21 63534.0 22 74285.0 23 68701.0 24 58573.0 25 57207.0 26 68448.0 27 89139.0 28 117189.0 29 155086.0 30 203551.0 31 267451.0 32 353182.0 33 464488.0 34 614091.0 35 858166.0 36 1389712.0 37 2005591.0 38 699566.0 39 685.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 42.36318789442416 19.130575419505575 13.063341121404997 25.442895564665264 2 15.742603717977783 21.18500237010779 37.52583932730158 25.546554584612846 3 17.90108787953843 26.271486499940817 29.908579375870925 25.918846244649828 4 13.170705884580416 17.53435695478222 36.654261983154825 32.64067517748254 5 13.850947885367592 37.17749073650456 33.72909273387492 15.242468644252924 6 31.09509360055631 37.285791878236004 17.414830983450162 14.20428353775752 7 26.624178275047033 31.98513704850449 22.89785307913972 18.492831597308758 8 27.02538883614548 32.54262008914731 20.267470272744614 20.164520801962595 9 27.00640176762515 13.748543369557334 20.079570953665144 39.16548390915237 10-11 25.31343045591301 25.062432356092597 27.40318364122219 22.220953546772197 12-13 26.64795466977537 23.022093113565255 27.29830217736907 23.031650039290298 14-15 24.130970350484162 23.480786413565617 25.72938159301569 26.65886164293453 16-17 23.125445947478553 25.95747769433424 26.600472856478312 24.31660350170889 18-19 23.42723850055184 24.887586983158045 29.11375098644346 22.57142352984665 20-21 24.430110343852263 24.312722444852223 28.3508906423658 22.906276568929712 22-23 23.963714336534903 24.017417303934582 28.117718398614734 23.90114996091578 24-25 23.591928029909315 24.616380235813924 28.20058802141112 23.591103712865642 26-27 23.819767972784142 24.40674610764576 28.085518514096403 23.6879674054737 28-29 23.312722831250838 24.359553956895688 28.647567498363923 23.680155713489548 30-31 23.90769297744991 24.27576341740208 28.322574063920374 23.49396954122764 32-33 23.57706456321565 24.46054567469899 27.893478402829057 24.0689113592563 34-35 23.845418400791445 24.440813585466156 28.406499842928962 23.307268170813433 36-37 24.066741087039762 24.340337065815145 27.877050021747802 23.715871825397286 38-39 24.109341534257524 23.975608973875975 28.000658938436786 23.91439055342972 40-41 23.828893420056637 24.111402326866695 28.17696618612847 23.882738066948196 42-43 24.407782943927252 24.163488860063552 27.528795390728213 23.899932805280987 44-45 24.05394316384979 24.547924996163356 27.20152288471222 24.19660895527463 46-47 23.936808113031383 24.518170588033 27.22678623385385 24.318235065081762 48-49 24.74751748550329 24.704227960756842 26.4551610812103 24.09309347252957 50-51 23.936022435849136 25.181642768556372 26.0336517129115 24.848683082682992 52-53 23.725793203929886 25.229247550259938 25.80097699802949 25.243982247780682 54-55 23.95466616898525 25.144046183392767 25.800479469160432 25.10080817846155 56-57 24.226184783648204 24.639809735227093 25.735358470772002 25.398647010352697 58-59 23.69733757186853 24.70180008946416 26.490336235058127 25.110526103609182 60-61 23.821159007795334 25.01192361723714 26.079047110105446 25.087870264862083 62-63 23.549218103084197 24.88282140024933 25.96819578766263 25.599764709003846 64-65 23.29823932319449 24.71432584454179 26.16102157611222 25.826413256151497 66-67 24.01179520409768 24.586743462103247 26.048090141129514 25.353371192669556 68-69 23.800727265982747 24.30247433239497 26.1402235894667 25.756574812155584 70-71 23.388931965306455 24.41707732374375 26.193575975208596 26.000414735741206 72-73 24.0492372298156 24.391193563423403 26.182634138600925 25.376935068160073 74-75 24.081984512370745 24.39215311998205 26.086124644722574 25.439737722924633 76-77 23.550731497656557 24.551800147423954 26.18338117592175 25.71408717899774 78-79 23.541773181811827 24.609250551764283 26.158878876026964 25.69009739039692 80-81 23.18998547448149 24.62496888653899 26.260115296101937 25.924930342877584 82-83 23.3990636015983 24.514931279650458 26.022471913006733 26.06353320574451 84-85 23.533002241240762 24.636530923545497 25.545900742181576 26.284566093032165 86-87 23.108665723081252 24.435011166275952 25.92214995280141 26.534173157841384 88-89 23.269787505234092 23.974192178957168 26.00764708617449 26.748373229634247 90-91 23.44610695899285 24.09797017005012 25.894858890268264 26.561063980688758 92-93 23.19516336858874 24.272995740809147 25.73139637265524 26.800444517946868 94-95 22.48233150123539 24.282173566346756 26.257441596484476 26.97805333593338 96-97 23.098406403982285 24.076937844326395 25.863012424746245 26.961643326945072 98-99 22.80286341981063 24.126909791250725 25.85748614471169 27.21274064422695 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 81.0 1 85.5 2 279.5 3 471.5 4 352.5 5 313.0 6 655.0 7 1672.5 8 3016.0 9 3082.5 10 1978.5 11 1381.0 12 1445.0 13 1636.5 14 1935.0 15 2451.0 16 3101.5 17 3769.0 18 4649.5 19 5720.5 20 6895.0 21 8505.0 22 10702.0 23 13165.0 24 15922.5 25 19404.0 26 24071.5 27 28833.0 28 35490.5 29 45619.0 30 54825.0 31 65480.5 32 78303.0 33 89422.0 34 99995.5 35 114235.5 36 125059.5 37 138945.5 38 156483.5 39 155628.5 40 154190.0 41 164045.5 42 175950.5 43 197783.5 44 225719.0 45 256784.0 46 292653.0 47 325872.0 48 347496.5 49 363530.5 50 385370.0 51 377230.5 52 356894.5 53 351194.5 54 329705.5 55 311493.0 56 286860.0 57 257413.0 58 240963.5 59 213240.5 60 176907.5 61 139945.5 62 114416.5 63 92254.0 64 68012.5 65 50656.5 66 36708.0 67 27789.5 68 21675.5 69 18482.0 70 16168.5 71 13801.5 72 13531.0 73 11220.5 74 7178.5 75 5352.0 76 4053.5 77 2627.0 78 1401.0 79 846.0 80 577.0 81 409.5 82 249.0 83 146.0 84 84.5 85 46.0 86 25.0 87 12.5 88 8.0 89 3.5 90 1.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05503604261873675 2 0.008281810298115547 3 0.0 4 0.0 5 0.0025115909924300645 6 0.0 7 0.0 8 0.0 9 0.004714063093484122 10-11 0.0 12-13 0.0 14-15 0.002575990761466733 16-17 0.004334104456167778 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 6.890775286923511E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.0607926091733864E-4 54-55 0.0 56-57 0.0011012360505270283 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0016872739487607102 70-71 2.962389375686743E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 5.731579444263481E-4 80-81 0.00103683628149036 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 3.541987297016758E-4 92-93 0.0011205559812380288 94-95 5.667179675226813E-4 96-97 2.769190068576738E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7764003.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.20440047193086 #Duplication Level Percentage of deduplicated Percentage of total 1 70.45201778968992 24.802210483238415 2 15.4851350696278 10.90289792706236 3 5.556868296221458 5.868786506098691 4 2.655563057595869 3.7395002143228067 5 1.453187091113998 2.5579290158108736 6 0.8572181063597561 1.8106709704847446 7 0.5982370989956641 1.4742404887146656 8 0.4201107630239235 1.1831798035250118 9 0.32964325838352787 1.0444403952905306 >10 1.8125784215572118 11.950966068309134 >50 0.1745485334016009 4.316695507128773 >100 0.1621634783797694 12.153221016102176 >500 0.02628069701563057 6.364461724181167 >1k 0.01567212490087646 9.426776285316983 >5k 5.914009394614298E-4 1.2828190942889053 >10k+ 1.8481279358169682E-4 1.1212045001248605 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 22026 0.28369386256033136 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20428 0.2631116963762121 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 19334 0.24902102691098907 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13733 0.17688040563611324 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10643 0.1370813483714522 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.2879953807333665E-5 0.0 5 0.0 1.2879953807333665E-5 0.0 2.575990761466733E-5 0.0 6 0.0 1.2879953807333665E-5 0.0 2.575990761466733E-5 0.0 7 0.0 1.2879953807333665E-5 0.0 2.575990761466733E-5 0.0 8 0.0 1.2879953807333665E-5 0.0 3.8639861422001E-5 0.0 9 0.0 1.2879953807333665E-5 0.0 5.151981522933466E-5 1.2879953807333665E-5 10-11 0.0 1.2879953807333665E-5 0.0 8.371969974766883E-5 1.2879953807333665E-5 12-13 1.93199307110005E-5 1.93199307110005E-5 0.0 1.0303963045866932E-4 2.575990761466733E-5 14-15 2.575990761466733E-5 2.575990761466733E-5 0.0 1.0947960736233615E-4 5.795979213300149E-5 16-17 3.219988451833417E-5 2.575990761466733E-5 0.0 1.1591958426600298E-4 6.439976903666832E-5 18-19 3.8639861422001E-5 2.575990761466733E-5 0.0 1.1591958426600298E-4 6.439976903666832E-5 20-21 3.8639861422001E-5 2.575990761466733E-5 0.0 2.769190068576738E-4 6.439976903666832E-5 22-23 3.8639861422001E-5 2.575990761466733E-5 0.0 0.001384595034288369 8.371969974766883E-5 24-25 3.8639861422001E-5 2.575990761466733E-5 0.0 0.004359864363782446 1.0303963045866932E-4 26-27 5.151981522933466E-5 2.575990761466733E-5 0.0 0.0074381733237351925 1.1591958426600298E-4 28-29 5.795979213300149E-5 2.575990761466733E-5 0.0 0.01866305306682648 1.1591958426600298E-4 30-31 7.083974594033517E-5 2.575990761466733E-5 0.0 0.05358704781541171 1.223595611696698E-4 32-33 7.7279722844002E-5 2.575990761466733E-5 0.0 0.11861793458863938 1.416794918806703E-4 34-35 7.7279722844002E-5 2.575990761466733E-5 0.0 0.20464314606782094 1.6099942259167083E-4 36-37 7.7279722844002E-5 2.575990761466733E-5 0.0 0.3369653515074634 1.6743939949533766E-4 38-39 7.7279722844002E-5 2.575990761466733E-5 0.0 0.5398503838805833 2.0607926091733864E-4 40-41 7.7279722844002E-5 2.575990761466733E-5 0.0 0.7848399852498769 2.0607926091733864E-4 42-43 8.371969974766883E-5 2.575990761466733E-5 0.0 1.0084166634144784 2.0607926091733864E-4 44-45 9.015967665133566E-5 2.575990761466733E-5 0.0 1.2644186252890424 2.0607926091733864E-4 46-47 1.0303963045866932E-4 2.575990761466733E-5 0.0 1.5475908497201765 2.0607926091733864E-4 48-49 1.0303963045866932E-4 2.575990761466733E-5 0.0 1.8474168544241931 2.3183916853200596E-4 50-51 1.0303963045866932E-4 2.575990761466733E-5 0.0 2.177394830991178 2.382791454356728E-4 52-53 1.0303963045866932E-4 2.575990761466733E-5 0.0 2.5079974853178184 3.219988451833416E-4 54-55 1.0303963045866932E-4 4.507983832566783E-5 0.0 2.8352384717007455 3.992785680273436E-4 56-57 1.0303963045866932E-4 6.439976903666832E-5 0.0 3.203663110382621 4.121585218346773E-4 58-59 1.0303963045866932E-4 6.439976903666832E-5 0.0 3.6081580597019345 4.2503847564201094E-4 60-61 1.0303963045866932E-4 6.439976903666832E-5 0.0 4.016169236410651 4.2503847564201094E-4 62-63 1.0303963045866932E-4 6.439976903666832E-5 0.0 4.428765676674777 4.379184294493446E-4 64-65 1.0303963045866932E-4 7.083974594033517E-5 0.0 4.8586598949021536 4.6367833706401193E-4 66-67 1.0303963045866932E-4 9.015967665133566E-5 0.0 5.325242661549718 4.894382446786793E-4 68-69 1.0947960736233615E-4 9.015967665133566E-5 0.0 5.829248133984493 5.02318198486013E-4 70-71 1.1591958426600298E-4 9.015967665133566E-5 0.0 6.392417674233253 5.409580599080139E-4 72-73 1.1591958426600298E-4 9.015967665133566E-5 0.0 6.94251406136757 5.473980368116808E-4 74-75 1.1591958426600298E-4 9.015967665133566E-5 0.0 7.47757954240873 5.538380137153476E-4 76-77 1.1591958426600298E-4 9.015967665133566E-5 0.0 8.052830479328769 5.538380137153476E-4 78-79 1.1591958426600298E-4 9.015967665133566E-5 0.0 8.646370950655223 5.667179675226812E-4 80-81 1.1591958426600298E-4 9.015967665133566E-5 0.0 9.287927889775416 5.924778751373485E-4 82-83 1.1591958426600298E-4 9.015967665133566E-5 0.0 9.965497179740915 6.18237782752016E-4 84-85 1.1591958426600298E-4 9.015967665133566E-5 0.0 10.625221293706353 6.375577134630165E-4 86-87 1.1591958426600298E-4 9.015967665133566E-5 0.0 11.298102280485981 6.504376672703501E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 53930 0.0 25.511757 1 AGTACTT 33695 0.0 21.711632 12-13 TATCACG 750 0.0 21.701574 2 GTACTAG 3230 0.0 21.607681 1 CAACGCA 64280 0.0 21.152592 5 ATCAACG 64635 0.0 21.029219 3 AACGCAG 67070 0.0 20.639606 6 TATCAAC 67090 0.0 20.538008 2 TCAACGC 68320 0.0 19.976635 4 TAGTACT 4275 0.0 19.688131 4 GTATAAG 3355 0.0 19.277102 1 ACGCAGA 70695 0.0 18.976145 7 CGCAGAG 70985 0.0 18.926043 8 TACTTTT 39315 0.0 18.885386 14-15 GAGTACT 32305 0.0 18.679567 12-13 TAGGCAT 4790 0.0 18.250906 5 TCTAACG 1535 0.0 18.177214 2 GCAGAGT 74235 0.0 17.991663 9 TCACGCA 980 0.0 17.55649 4 ATTTAGG 4240 0.0 17.448204 1 >>END_MODULE