FastQCFastQC Report
Fri 27 May 2016
ERR765486_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765486_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16754146
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169950.10143757849549598No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA435800.030.0619531
ATCAACG503900.024.2463463
CAACGCA504400.024.2223115
AACGCAG535850.023.1073556
GTACATG795700.022.9090141
TACATGG787100.022.418692
TATCAAC589550.022.0937372
TCAACGC560850.021.842924
GTACTAG58000.021.5354441
ACGCAGA580750.020.9004047
CGCAGAG576850.020.805658
ACATGGG825200.020.2114333
CATGGGG390700.019.9742744
GGTATCA296200.019.1822931
GCAGAGT597450.019.1296739
TAGTACT68600.018.2755534
CTAGTAC72300.017.4701793
AGAGTAC548950.017.29714810-11
GTATAAG60050.017.2814221
TCTAACG31400.016.7483392
Produced by FastQC (version 0.11.5)