##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765486_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	16754146
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.26844483747485	33.0	33.0	33.0	33.0	33.0
2	32.205781363013074	33.0	33.0	33.0	33.0	33.0
3	32.23041448964334	33.0	33.0	33.0	33.0	33.0
4	36.06382885764515	37.0	37.0	37.0	33.0	37.0
5	36.07346593493933	37.0	37.0	37.0	33.0	37.0
6	36.04191308825887	37.0	37.0	37.0	33.0	37.0
7	36.009096196248976	37.0	37.0	37.0	33.0	37.0
8	35.974096739994984	37.0	37.0	37.0	33.0	37.0
9	35.985459479701326	37.0	37.0	37.0	33.0	37.0
10-11	35.95629443004734	37.0	37.0	37.0	33.0	37.0
12-13	35.9371272042156	37.0	37.0	37.0	33.0	37.0
14-15	37.82220547081302	40.0	37.0	40.0	33.0	40.0
16-17	37.86248415765268	40.0	37.0	40.0	33.0	40.0
18-19	37.89936338742661	40.0	37.0	40.0	33.0	40.0
20-21	37.907370927769165	40.0	37.0	40.0	33.0	40.0
22-23	37.88882175790995	40.0	37.0	40.0	33.0	40.0
24-25	37.88197727296873	40.0	37.0	40.0	33.0	40.0
26-27	37.85987525117663	40.0	37.0	40.0	33.0	40.0
28-29	37.81981659942559	40.0	37.0	40.0	33.0	40.0
30-31	37.76629286267411	40.0	37.0	40.0	33.0	40.0
32-33	37.70101403557066	40.0	37.0	40.0	33.0	40.0
34-35	37.6062507453379	40.0	37.0	40.0	33.0	40.0
36-37	37.51717980731456	40.0	37.0	40.0	33.0	40.0
38-39	37.413897282499505	40.0	37.0	40.0	33.0	40.0
40-41	37.3170465089656	40.0	37.0	40.0	33.0	40.0
42-43	37.17640364958022	40.0	37.0	40.0	33.0	40.0
44-45	37.021829939884725	40.0	37.0	40.0	33.0	40.0
46-47	36.84364061289665	38.5	37.0	40.0	33.0	40.0
48-49	36.66820385831662	37.0	37.0	40.0	33.0	40.0
50-51	36.45770954246191	37.0	37.0	40.0	33.0	40.0
52-53	36.31103683231601	37.0	37.0	40.0	33.0	40.0
54-55	36.138688835587324	37.0	37.0	40.0	33.0	40.0
56-57	35.96732749016273	37.0	37.0	40.0	33.0	40.0
58-59	35.76430622008427	37.0	37.0	40.0	33.0	40.0
60-61	35.60853325499252	37.0	37.0	40.0	30.0	40.0
62-63	35.405276311905126	37.0	33.0	40.0	27.0	40.0
64-65	35.20429077674267	37.0	33.0	37.0	27.0	40.0
66-67	35.01942375934888	37.0	33.0	37.0	27.0	40.0
68-69	34.79540297070349	37.0	33.0	37.0	27.0	40.0
70-71	34.60993096872858	37.0	33.0	37.0	27.0	40.0
72-73	34.413002578585626	37.0	33.0	37.0	27.0	38.5
74-75	34.21328371496823	37.0	33.0	37.0	27.0	37.0
76-77	34.03812196694478	37.0	33.0	37.0	27.0	37.0
78-79	33.88660958905336	37.0	33.0	37.0	27.0	37.0
80-81	33.727314182411924	37.0	33.0	37.0	27.0	37.0
82-83	33.59689273329718	37.0	33.0	37.0	27.0	37.0
84-85	33.46586447915638	37.0	33.0	37.0	27.0	37.0
86-87	33.36553164213801	37.0	33.0	37.0	27.0	37.0
88-89	33.294504029032574	37.0	33.0	37.0	27.0	37.0
90-91	33.188161664581415	37.0	33.0	37.0	27.0	37.0
92-93	33.12061292172099	37.0	33.0	37.0	27.0	37.0
94-95	33.0311309212657	37.0	33.0	37.0	27.0	37.0
96-97	32.95859084670744	37.0	33.0	37.0	27.0	37.0
98-99	32.87123327563219	37.0	33.0	37.0	27.0	37.0
100	32.80344369686166	37.0	33.0	37.0	27.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	40.0
5	79.0
6	513.0
7	1187.0
8	2090.0
9	3630.0
10	3974.0
11	4902.0
12	6877.0
13	10731.0
14	18066.0
15	25364.0
16	30265.0
17	37342.0
18	45932.0
19	55676.0
20	65793.0
21	80470.0
22	97627.0
23	100913.0
24	94092.0
25	96646.0
26	108161.0
27	125628.0
28	153555.0
29	197043.0
30	255226.0
31	336280.0
32	456462.0
33	630087.0
34	877647.0
35	1316182.0
36	2557803.0
37	5667144.0
38	3286351.0
39	4362.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.16757270150429	18.95410113407397	12.643502616129323	25.234823548292418
2	16.9432988856938	21.04820431241018	36.5291632070106	25.47933359488542
3	18.429570262852643	25.266672006652463	29.414617387431445	26.88914034306345
4	13.63565173659105	17.21327365775612	36.16961437485384	32.981460230798994
5	14.941893366429174	36.56309055748477	33.0451056171427	15.449910458943354
6	31.940913887286133	36.3377898085146	17.437648034456952	14.283648269742319
7	27.990611995383112	31.42563040813898	21.912289650573655	18.671467945904254
8	27.132239387194073	32.08220222027431	20.231869771219614	20.553688621312002
9	27.215890323505597	14.4594657346307	19.685933260937322	38.63871068092638
10-11	25.71812374083406	24.833539710111157	27.173512753201507	22.274823795853276
12-13	26.574938525664038	23.010698366840067	26.6373588961204	23.777004211375502
14-15	24.432258508124338	24.107057590188052	24.280247991775628	27.18043590991198
16-17	24.209162199083927	26.340974677062366	24.964241729786437	24.48562139406727
18-19	24.348495791798484	25.611197994389062	26.71581806243658	23.32448815137587
20-21	25.78497668975369	24.899779238668167	25.665412932699628	23.649831138878515
22-23	25.149595459346425	24.596303380131857	25.69013225642493	24.563968904096786
24-25	24.569799198777343	25.199946682675794	25.844868379199614	24.385385739347246
26-27	24.690923022894275	25.314766902331865	25.650427711619546	24.34388236315431
28-29	24.34916097075247	25.15238974608551	26.139707084331377	24.358742198830637
30-31	24.72699037446848	25.02656706892079	25.93942863544273	24.307013921168
32-33	24.505423480263207	25.09654109896338	25.485369047635043	24.912666373138368
34-35	24.872814698952066	25.057383164091828	25.88811742345241	24.181684713503692
36-37	24.759426843631278	25.044185961723347	25.547265878004495	24.649121316640883
38-39	24.781463895676005	24.791273295308496	25.796971167949824	24.63029164106568
40-41	24.710227187569547	24.968151010219803	25.96323675662726	24.358385045583393
42-43	24.986364678265975	24.91697210971711	25.69174083922401	24.404922372792907
44-45	24.672718734690786	25.188971371368694	25.367653996201707	24.770655897738816
46-47	24.46199501129989	25.109767674184646	25.503328312985474	24.92490900152999
48-49	25.01963977925109	25.25969977006665	25.132262874556726	24.58839757612553
50-51	24.48592416084196	25.45636120262829	25.08328199361855	24.974432642911196
52-53	24.640673305105796	25.209082291360158	25.06974385516063	25.080500548373415
54-55	24.76347522351457	25.169108795186208	25.13447960906196	24.932936372237265
56-57	24.722077550771317	24.965596326166533	24.91037966870961	25.401946454352537
58-59	24.441084925306512	25.037519164137574	25.34447677239349	25.176919138162425
60-61	24.591544792007067	25.09408434606726	25.15593591930991	25.158434942615767
62-63	24.350278530847362	25.09954822790616	25.02834415758393	25.521829083662546
64-65	24.32722064065428	24.942782785587003	25.175254913455987	25.55474166030273
66-67	24.66128463697451	25.056121189049147	25.02673063071153	25.255863543264812
68-69	24.606592813479256	24.804801720939075	25.131385495070003	25.457219970511662
70-71	24.168517907790903	24.836155979686474	25.247997043985464	25.747329068537155
72-73	24.62892384586675	24.894607929916265	25.16394603959447	25.31252218462251
74-75	24.758512908848676	24.894735094766478	25.15038568669572	25.19636630968912
76-77	24.478132194358903	25.08087927260591	25.106896788419224	25.33409174461596
78-79	24.516517406269045	25.107313192225924	25.080926757970058	25.295242643534976
80-81	24.301595915052626	24.932124180217276	25.42981302142803	25.336466883302073
82-83	24.535892150286745	24.94876080748245	25.217174985107256	25.298172057123548
84-85	24.55859090133446	25.003531654912596	24.926321856937587	25.51155558681536
86-87	24.248742387644764	24.851817196306932	25.17608662588236	25.72335379016594
88-89	24.334391335508762	24.57088283722696	25.263837594700856	25.83088823256342
90-91	24.501067049336203	24.712832179440284	25.134717702511765	25.65138306871175
92-93	24.383720590451507	24.706538411497178	25.10339414192242	25.806346856128897
94-95	23.92971610952862	24.829817971753478	25.44725342854125	25.793212490176654
96-97	24.326366704068644	24.72433316597807	25.14870874832261	25.80059138163068
98-99	24.015315782886983	24.79777431841068	25.182220919844166	26.00468897885817
100	24.038892685183992	24.799787216465702	25.071592395376257	26.089727702974052
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	715.0
1	588.5
2	643.0
3	805.0
4	659.0
5	652.0
6	936.0
7	1722.0
8	2741.5
9	2820.0
10	2079.0
11	1708.0
12	1857.0
13	2133.0
14	2500.5
15	2962.0
16	3505.0
17	4193.0
18	5074.5
19	6132.5
20	7650.5
21	9562.0
22	12181.0
23	16262.0
24	21620.5
25	28992.5
26	40821.0
27	54566.0
28	67703.0
29	85596.5
30	104464.5
31	122457.0
32	143457.0
33	167712.0
34	194152.0
35	214877.5
36	238800.5
37	268135.5
38	288621.5
39	310356.0
40	330547.5
41	346939.5
42	365416.0
43	405747.5
44	461003.5
45	510067.0
46	574242.5
47	637065.5
48	687747.0
49	720956.0
50	736445.5
51	735252.0
52	736313.0
53	748191.0
54	730100.0
55	697493.5
56	670701.0
57	623417.5
58	584957.0
59	544121.5
60	466191.5
61	381284.0
62	314667.5
63	251316.5
64	190569.0
65	154196.0
66	128618.0
67	101753.0
68	80153.5
69	65650.0
70	56229.0
71	49057.5
72	42699.5
73	42542.0
74	33909.5
75	24566.5
76	19467.0
77	15310.5
78	11742.0
79	7429.0
80	5553.0
81	4666.0
82	4253.5
83	4134.0
84	3360.5
85	2277.0
86	1576.5
87	847.5
88	383.5
89	277.0
90	202.5
91	145.5
92	101.0
93	74.5
94	61.5
95	37.5
96	16.0
97	9.0
98	9.0
99	8.0
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02968817390035875
2	0.00186819429650428
3	2.62621562447886E-4
4	0.0
5	0.0022621266401761093
6	1.372794530977586E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.5068421870025483E-4
16-17	0.004052728202320787
18-19	0.006840097967392668
20-21	0.01940116792583758
22-23	0.025202716987186336
24-25	0.032042814954579
26-27	0.031299715306288965
28-29	0.04690480792037983
30-31	0.046854074209452395
32-33	0.05726045362144988
34-35	0.05132162510700336
36-37	0.055457914715557576
38-39	0.05969865608190355
40-41	0.04265809788216003
42-43	0.04844472526382425
44-45	0.04592296139713716
46-47	0.043562351671043095
48-49	0.03729823053947363
50-51	0.0371728884301235
52-53	0.03840243483612952
54-55	0.057299249988629675
56-57	0.04409654780374959
58-59	0.04399508038189472
60-61	0.04538279659255685
62-63	0.030461116908017873
64-65	0.03265758815758201
66-67	0.033245502337152844
68-69	0.030744628822024115
70-71	0.0434101505382608
72-73	0.03279785194661668
74-75	0.04102865046060838
76-77	0.04617066127990051
78-79	0.041207710616822844
80-81	0.03419750550102643
82-83	0.021132082769244102
84-85	0.033567810618338884
86-87	0.027745371205431777
88-89	0.02683216440873799
90-91	0.022376550854934653
92-93	0.01547378183286692
94-95	0.015503625192236
96-97	0.014071143942520258
98-99	0.011247962146205482
100	0.005789611717601124
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.6754146E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.471484977305856
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.29441314026212	26.715075309511693
2	15.142822728454483	11.348481087665755
3	5.476067276421424	6.1558911804942475
4	2.5222846402564145	3.780550040234302
5	1.40022295935061	2.6234216793092564
6	0.8544645101640553	1.9210832433752045
7	0.5606722446622026	1.4706455115136454
8	0.4185669602426439	1.2547460450183048
9	0.29447449368661727	0.9930956909740045
>10	1.716842066461055	11.956001394826162
>50	0.1603772601117158	4.1944010152565925
>100	0.12424025056648211	9.46801405550579
>500	0.01843793948495595	4.849202293106839
>1k	0.015026559594803838	10.508667613489278
>5k	0.0010230308521844934	2.462170077101139
>10k+	6.393942826153084E-5	0.2985537626179665
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16995	0.10143757849549598	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.7906015621446774E-5
2	0.0	0.0	0.0	0.0	1.7906015621446774E-5
3	0.0	0.0	0.0	0.0	1.7906015621446774E-5
4	0.0	0.0	0.0	1.1937343747631184E-5	1.7906015621446774E-5
5	0.0	0.0	0.0	1.1937343747631184E-5	1.7906015621446774E-5
6	5.968671873815592E-6	0.0	0.0	1.1937343747631184E-5	2.3874687495262368E-5
7	5.968671873815592E-6	0.0	0.0	1.1937343747631184E-5	2.3874687495262368E-5
8	1.1937343747631184E-5	0.0	0.0	1.1937343747631184E-5	2.3874687495262368E-5
9	1.1937343747631184E-5	0.0	0.0	6.565539061197151E-5	2.3874687495262368E-5
10-11	1.1937343747631184E-5	0.0	0.0	7.75927343596027E-5	3.879636717980135E-5
12-13	2.089035155835457E-5	0.0	0.0	1.0146742185486506E-4	6.267105467506372E-5
14-15	3.581203124289355E-5	0.0	0.0	1.2534210935012744E-4	8.057707029651049E-5
16-17	4.7749374990524736E-5	0.0	0.0	1.6712281246683656E-4	9.251441404414167E-5
18-19	5.9686718738155917E-5	0.0	0.0	1.8502882808828333E-4	1.0445175779177286E-4
20-21	7.75927343596027E-5	0.0	0.0	2.208408593311769E-4	1.2534210935012744E-4
22-23	8.356140623341828E-5	0.0	0.0	7.699586717222113E-4	1.46232460908482E-4
24-25	9.549874998104947E-5	0.0	0.0	0.0021099255073938118	1.6712281246683656E-4
26-27	1.2235777341321964E-4	0.0	0.0	0.0035453910930464613	1.8801316402519114E-4
28-29	1.372794530977586E-4	0.0	0.0	0.01150759937271646	2.0591917964663792E-4
30-31	1.492167968453898E-4	0.0	0.0	0.039348469328129285	2.148721874573613E-4
32-33	1.492167968453898E-4	0.0	0.0	0.09316201494245066	2.3874687495262367E-4
34-35	1.492167968453898E-4	0.0	0.0	0.1674839171152024	2.3874687495262367E-4
36-37	1.522011327822976E-4	0.0	0.0	0.2830881383031997	2.3874687495262367E-4
38-39	1.6712281246683656E-4	0.0	0.0	0.44717886545813795	2.5366855463716267E-4
40-41	1.6712281246683656E-4	0.0	0.0	0.6299097548750023	2.685902343217016E-4
42-43	1.7010714840374436E-4	0.0	0.0	0.8054543633557927	2.954492577538718E-4
44-45	1.8502882808828333E-4	0.0	0.0	1.0166916296420003	3.2230828118604194E-4
46-47	2.0591917964663792E-4	0.0	0.0	1.253334547759104	3.491673046182121E-4
48-49	2.0890351558354572E-4	0.0	0.0	1.4919859239617463	3.9393234367182905E-4
50-51	2.0890351558354572E-4	0.0	0.0	1.7300344643051337	3.9990101554564467E-4
52-53	2.178565233942691E-4	0.0	0.0	1.9589718270331415	4.1780703116709144E-4
54-55	2.208408593311769E-4	0.0	0.0	2.205654051242003	4.327287108516304E-4
56-57	2.208408593311769E-4	0.0	0.0	2.4914012328649875	4.53619062409985E-4
58-59	2.2382519526808467E-4	0.0	0.0	2.794729137492296	4.7450941396833955E-4
60-61	2.2680953120499247E-4	0.0	0.0	3.097460174932223	4.8346242177906294E-4
62-63	2.2979386714190028E-4	0.0	0.0	3.4046498102618896	4.953997655266941E-4
64-65	2.3277820307880808E-4	0.0	0.0	3.72578226308879	5.192744530219564E-4
66-67	2.3277820307880808E-4	0.0	0.0	4.079849847315405	5.431491405172189E-4
68-69	2.447155468264393E-4	0.0	0.0	4.465494690090441	5.491178123910344E-4
70-71	2.4769988276334705E-4	0.0	0.0	4.8784790343834885	5.580708202017578E-4
72-73	2.5068421870025483E-4	0.0	0.0	5.284366627818571	5.938828514446513E-4
74-75	2.5068421870025483E-4	0.0	0.0	5.697855324884957	6.088045311291903E-4
76-77	2.5068421870025483E-4	0.0	0.0	6.151110298310639	6.207418748768216E-4
78-79	2.5068421870025483E-4	0.0	0.0	6.625759976068013	6.207418748768216E-4
80-81	2.5366855463716267E-4	5.968671873815592E-6	0.0	7.1339834331156	6.296948826875449E-4
82-83	2.6560589838479383E-4	5.968671873815592E-6	0.0	7.659793581839384	6.505852342458996E-4
84-85	2.685902343217016E-4	1.1937343747631184E-5	0.0	8.185848446109997	6.595382420566229E-4
86-87	2.685902343217016E-4	1.1937343747631184E-5	0.0	8.745536776389557	6.893816014257009E-4
88	2.685902343217016E-4	1.1937343747631184E-5	0.0	9.179310004819106	7.043032811102398E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	43580	0.0	30.061953	1
ATCAACG	50390	0.0	24.246346	3
CAACGCA	50440	0.0	24.222311	5
AACGCAG	53585	0.0	23.107355	6
GTACATG	79570	0.0	22.909014	1
TACATGG	78710	0.0	22.41869	2
TATCAAC	58955	0.0	22.093737	2
TCAACGC	56085	0.0	21.84292	4
GTACTAG	5800	0.0	21.535444	1
ACGCAGA	58075	0.0	20.900404	7
CGCAGAG	57685	0.0	20.80565	8
ACATGGG	82520	0.0	20.211433	3
CATGGGG	39070	0.0	19.974274	4
GGTATCA	29620	0.0	19.182293	1
GCAGAGT	59745	0.0	19.129673	9
TAGTACT	6860	0.0	18.275553	4
CTAGTAC	7230	0.0	17.470179	3
AGAGTAC	54895	0.0	17.297148	10-11
GTATAAG	6005	0.0	17.281422	1
TCTAACG	3140	0.0	16.748339	2
>>END_MODULE