FastQCFastQC Report
Fri 27 May 2016
ERR765485_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765485_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6963618
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA249450.35821896031631834No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT222250.3191588050924103No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA103780.14903172460063147No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG101830.1462314561195057No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT98940.14208131462696547No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA95240.13676798468841916No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA93440.1341831214750723No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA89250.12816613432844823No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA85650.12299640790175452No Hit
GTACATGGGTACCTGGTTGATCCTGCCAGTAGCATATGCTTGTCTCAAAG83970.12058386890263079No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG82610.1186308611414354No Hit
GTCCTATTCCATTATTCCTAGCTGCGGTATCCAGGCGGCTCGGGCCTGCT79140.11364781928015007No Hit
ATACAGGACTCTTTCGAGGCCCTGTAATTGGAATGAGTCCACTTTAAATC75980.10910994830560782No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC75480.10839193074634479No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT75450.10834884969278899No Hit
CTTTAATATACGCTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGA73960.10620915736618522No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73300.10526137418795803No Hit
ATATTAAAGTTGCTGCAGTTAAAAAGCTCGTAGTTGGATCTTGGGAGCGG72240.10373917696232045No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT70950.10188669165942187No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC69790.10022089092193166No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG20100.026.1415421
GTATAAG25650.021.7541771
GTACATA17250.021.2954481
GTACAAG28800.020.1820981
ATGGGTA43700.019.8969785
TAGTACT30400.019.2718734
CTAGGCA33650.019.0686974
CATGGGG166150.018.7500084
TAGGCAT35150.018.5195185
GGGTACC45100.018.1440837
CATGGGT58200.017.656164
GTACAAA32800.017.57911
TCTAACG11150.017.5155282
GGTACCT46800.017.484758
TGGGTAC48150.017.4775756
GTGATCG16050.017.3807648
CTAGTAC30900.017.3056583
GTACATG662600.016.6039771
TACATGG649750.016.4528642
CTAACGC11400.016.3156343
Produced by FastQC (version 0.11.5)