FastQCFastQC Report
Fri 27 May 2016
ERR765484_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765484_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12489792
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT414430.33181497337986093No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA399090.3195329433828842No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201870.16162799188329158No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG153630.12300445035433737No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA133810.10713549112747435No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG132140.10579839920472654No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA37600.026.1266482
GTACTAG43400.024.2631931
GTACAAG54150.022.745341
GTACATA29250.022.5004791
GTATAAG49350.021.9093911
CATGGGT104100.019.8649374
TAGTACT49650.018.8373184
TAGGCAT67000.018.7979495
CATGGGG314250.018.6498954
AGGGCTA59400.018.3548985
ATGGGTA76200.018.193555
GGGCTAT63200.017.8448686
CTAGGCA73300.017.8248584
TTTAGGG65700.017.6708262
GTACATG1249850.017.6665731
ATTTAGG64800.017.4836331
CTGTGCG60650.017.1972059
TACATGG1234300.017.0867942
GTACAAA50450.017.0521661
GTCCTAC43500.016.9668031
Produced by FastQC (version 0.11.5)