##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765484_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12489792
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73752749445307	33.0	33.0	33.0	27.0	33.0
2	31.61156967225715	33.0	33.0	33.0	27.0	33.0
3	31.576015757508213	33.0	33.0	33.0	27.0	33.0
4	35.176742895318036	37.0	37.0	37.0	33.0	37.0
5	35.40688692013446	37.0	37.0	37.0	33.0	37.0
6	35.39187770300738	37.0	37.0	37.0	33.0	37.0
7	35.247107317719944	37.0	37.0	37.0	33.0	37.0
8	35.19854477960882	37.0	37.0	37.0	33.0	37.0
9	34.87122956090862	37.0	37.0	37.0	33.0	37.0
10-11	34.90710181562672	37.0	37.0	37.0	33.0	37.0
12-13	34.883169791778755	37.0	37.0	37.0	33.0	37.0
14-15	36.173097438292004	37.0	37.0	40.0	30.0	40.0
16-17	36.440239357068556	37.0	37.0	40.0	33.0	40.0
18-19	36.26079613655696	37.0	37.0	40.0	33.0	40.0
20-21	36.26408522255615	37.0	37.0	40.0	30.0	40.0
22-23	36.45916733441197	37.0	37.0	40.0	33.0	40.0
24-25	36.47886874337058	37.0	37.0	40.0	33.0	40.0
26-27	36.14645692258125	37.0	37.0	40.0	27.0	40.0
28-29	36.019387072258695	37.0	37.0	40.0	27.0	40.0
30-31	35.80837234919525	37.0	37.0	40.0	27.0	40.0
32-33	35.75760192803851	37.0	37.0	40.0	27.0	40.0
34-35	35.68469354813915	37.0	37.0	40.0	27.0	40.0
36-37	35.425005756701154	37.0	33.0	40.0	27.0	40.0
38-39	35.253519994568364	37.0	33.0	40.0	27.0	40.0
40-41	35.36902419992262	37.0	33.0	40.0	27.0	40.0
42-43	35.258068469034555	37.0	33.0	40.0	27.0	40.0
44-45	35.178014894083105	37.0	33.0	40.0	27.0	40.0
46-47	35.10527405100101	37.0	33.0	40.0	27.0	40.0
48-49	34.90794146131496	37.0	33.0	40.0	27.0	40.0
50-51	34.72831729303418	37.0	33.0	40.0	27.0	40.0
52-53	34.6013509672539	37.0	33.0	40.0	27.0	40.0
54-55	34.45199928069259	37.0	33.0	40.0	27.0	40.0
56-57	34.227184367842156	37.0	33.0	38.5	27.0	40.0
58-59	34.03844771794438	37.0	33.0	37.0	27.0	40.0
60-61	33.764098193148456	37.0	33.0	37.0	27.0	40.0
62-63	33.53082817552126	37.0	33.0	37.0	24.5	40.0
64-65	33.337711869020715	37.0	33.0	37.0	22.0	40.0
66-67	33.11772473873064	37.0	33.0	37.0	22.0	40.0
68-69	32.91700202053005	37.0	33.0	37.0	22.0	38.5
70-71	32.696980702320744	37.0	33.0	37.0	22.0	37.0
72-73	32.46950137360174	35.0	33.0	37.0	22.0	37.0
74-75	32.25173409613227	33.0	33.0	37.0	22.0	37.0
76-77	32.05895546539126	33.0	33.0	37.0	22.0	37.0
78-79	31.885208576732104	33.0	33.0	37.0	22.0	37.0
80-81	31.75052434820372	33.0	33.0	37.0	22.0	37.0
82-83	31.62869085409909	33.0	33.0	37.0	22.0	37.0
84-85	31.461856210255544	33.0	33.0	37.0	22.0	37.0
86-87	31.253669516674098	33.0	30.0	37.0	22.0	37.0
88-89	31.11591694241185	33.0	27.0	37.0	18.5	37.0
90-91	31.02680625105686	33.0	27.0	37.0	15.0	37.0
92-93	30.933768512718224	33.0	27.0	37.0	15.0	37.0
94-95	30.80197948852951	33.0	27.0	37.0	15.0	37.0
96-97	30.707784765350773	33.0	27.0	37.0	15.0	37.0
98-99	30.563412945547853	33.0	27.0	37.0	15.0	37.0
100	30.474122947764062	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	7.0
7	205.0
8	1372.0
9	2618.0
10	3981.0
11	6866.0
12	13201.0
13	23458.0
14	36600.0
15	46341.0
16	52068.0
17	60635.0
18	70788.0
19	81821.0
20	94710.0
21	110890.0
22	125730.0
23	127525.0
24	125929.0
25	136096.0
26	157588.0
27	190739.0
28	235114.0
29	293457.0
30	368099.0
31	459286.0
32	592768.0
33	783142.0
34	1031122.0
35	1418319.0
36	2132263.0
37	2787960.0
38	918334.0
39	760.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.31105977604789	18.968231983787962	12.736412892410035	24.98429534775411
2	16.028358650143502	21.078744138430935	37.1610004331833	25.731896778242263
3	17.736399003409232	26.641873357437923	29.28456388663031	26.337163752522528
4	12.455280008232652	17.355861539305504	36.78385247217776	33.40500598028408
5	14.19141406764503	37.396394682287706	32.72486630125332	15.687324948813943
6	31.466253444794752	36.71722142300946	17.246778204430942	14.569746927764843
7	27.36427056744929	32.05078827176199	22.207676814024772	18.377264346763955
8	27.468327459751897	31.918309055516414	20.106737359428994	20.506626125302695
9	27.35638550771008	14.200110270974184	19.93601849400852	38.50748572730722
10-11	25.609379781515706	24.959974384887403	26.254489570520906	23.17615626307598
12-13	26.64835145038498	22.96485119837561	26.146630620719698	24.240166730519714
14-15	24.684922931964202	23.892556329789123	24.205219366949027	27.217301371297648
16-17	23.979371335380346	26.06645523946903	25.246108904824332	24.708064520326296
18-19	24.474855418294883	24.9878134044864	27.473494555105994	23.063836622112728
20-21	25.881387408816504	24.381570494457748	26.136562270617475	23.600479826108277
22-23	24.80166993214429	24.237611775691498	26.020024819311637	24.940693472852573
24-25	24.281794647451363	25.098359638251484	26.046718175320294	24.57312753897686
26-27	24.64245691601283	24.715401315329757	26.162696524108874	24.479445244548543
28-29	23.97333385879979	24.63147258931448	26.967191027724287	24.428002524161442
30-31	24.71581617977647	24.685174029073156	26.46479773176796	24.134212059382413
32-33	24.0342240535797	24.878827956844553	25.834467519036057	25.252480470539684
34-35	24.62988829221008	24.869984782594244	26.65769552387824	23.842431401317445
36-37	24.758033286090587	24.87781361312809	25.975169142101397	24.388983958679933
38-39	24.908912344721998	24.23662831057559	26.12208459521024	24.732374749492177
40-41	24.469840313637025	24.92401399638875	26.36861723177193	24.23752845820229
42-43	25.408920362121084	24.657636471350237	25.829659208664246	24.10378395786443
44-45	24.675936492565157	25.120646838010984	25.412607419326903	24.790809250096956
46-47	24.168998704527265	25.041107424893426	26.068172149659546	24.721721720919763
48-49	25.31272135013679	25.300491385786756	25.128614919883518	24.25817234419294
50-51	24.13884325956781	25.832093202271693	24.84247121808682	25.186592320073668
52-53	24.16086721320263	25.410863760372553	24.67652987094342	25.751739155481403
54-55	24.62485364047696	25.323964562420255	24.831910731579836	25.219271065522946
56-57	24.87910527252976	24.680350961809452	24.45172825936573	25.98881550629506
58-59	24.306197413055397	24.748286440638882	25.44955512469703	25.495961021608686
60-61	24.41633135283598	25.135794895543494	25.05988090113911	25.387992850481417
62-63	23.84804044414774	25.230973989965182	24.82446761408064	26.096517951806437
64-65	23.933790286024916	24.833907049598235	25.078451213887593	26.153851450489256
66-67	24.780294815383993	24.871749819461936	24.85922754969806	25.488727815456013
68-69	24.885435112777806	24.373238891379394	24.830197362663654	25.911128633179143
70-71	23.845778955237588	24.625552061055966	25.270703175322367	26.257965808384082
72-73	24.813763554295004	24.725631412849303	25.075942163217125	25.384662869638568
74-75	25.187407149122265	24.413666848548633	25.045723365133988	25.353202637195118
76-77	24.45643860594668	24.863051036004997	25.31583721587421	25.364673142174116
78-79	24.58859399648881	24.873002278420834	25.213912706785884	25.324491018304467
80-81	24.05058436540316	24.582314767872763	25.678150021441514	25.68895084528256
82-83	24.330784099557988	24.83553334786256	24.943401661808558	25.89028089077089
84-85	24.411736977090474	24.936550578087616	24.55776562596017	26.093946818861745
86-87	24.178698627041197	24.39224473061648	24.893376075382044	26.53568056696028
88-89	24.282897572649436	23.96132297405621	25.19171832713792	26.564061126156442
90-91	24.7169501622186	24.118594927506063	24.934191773451236	26.2302631368241
92-93	24.205414964697674	24.38639339721371	24.818882448771184	26.58930918931743
94-95	23.331510060355367	24.626131290306787	25.484127816893697	26.558230832444153
96-97	24.39979550879997	24.38913058012168	24.765321527182557	26.445752383895798
98-99	23.806403037037413	24.419721190414833	24.92483852046994	26.849037252077814
100	23.71113269027151	24.755713157444674	24.64707717318408	26.886076979099744
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1055.0
1	930.0
2	1095.0
3	1389.5
4	1227.0
5	1129.0
6	1420.5
7	2577.5
8	4240.5
9	4254.5
10	2797.5
11	1968.5
12	1867.5
13	1973.0
14	2286.0
15	2840.0
16	3463.0
17	4167.5
18	5137.5
19	6431.0
20	8058.0
21	10121.0
22	12830.0
23	15993.0
24	19691.5
25	24561.0
26	31629.5
27	39678.0
28	47553.0
29	59662.5
30	74761.5
31	88913.0
32	106117.5
33	122726.5
34	137889.0
35	151238.0
36	162751.5
37	182149.5
38	194184.0
39	198587.0
40	203265.0
41	205182.0
42	217275.0
43	254516.0
44	305191.5
45	353253.0
46	416110.0
47	477969.0
48	545104.0
49	600429.5
50	646587.0
51	638408.5
52	583919.0
53	573748.0
54	556412.0
55	526492.0
56	504500.5
57	471357.0
58	451397.0
59	424132.5
60	362664.0
61	293581.5
62	240133.0
63	188655.0
64	138241.5
65	110297.0
66	86120.0
67	61042.5
68	46735.0
69	39775.5
70	36671.0
71	34283.5
72	31170.5
73	33562.5
74	27626.0
75	19763.5
76	14341.5
77	9546.0
78	6716.5
79	3849.5
80	2409.5
81	1777.5
82	1406.5
83	1091.5
84	782.5
85	532.0
86	371.5
87	204.5
88	113.0
89	81.0
90	52.0
91	36.5
92	24.0
93	20.0
94	15.5
95	11.0
96	10.0
97	5.0
98	1.5
99	1.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04988073460310628
2	0.025308668070693253
3	0.02258644499444026
4	0.023435138071154427
5	0.016141181534488326
6	0.025612916532156818
7	0.050281061526084664
8	0.006557354998385882
9	0.004467648460438733
10-11	0.00232189615327461
12-13	0.0014171573073434689
14-15	0.0024660138455468274
16-17	0.006881619805998371
18-19	0.00528431538331463
20-21	0.008815198843983952
22-23	0.01872329018769888
24-25	0.025757034224429036
26-27	0.028579339031426627
28-29	0.04520491614271879
30-31	0.042875013450984614
32-33	0.040545110759250436
34-35	0.04852762960343936
36-37	0.045545194027250414
38-39	0.05523310556332724
40-41	0.05261897075627841
42-43	0.05786325344729519
44-45	0.058327632677950124
46-47	0.06294740536912063
48-49	0.06527730806085483
50-51	0.03375956941476688
52-53	3.002451922337858E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.92483846008004E-4
64-65	1.881536537998391E-4
66-67	3.482844229911915E-4
68-69	0.001160948076637305
70-71	4.003269229783811E-5
72-73	1.921569230296229E-4
74-75	5.6045769216973344E-5
76-77	9.207519228502765E-5
78-79	8.006538459567622E-6
80-81	3.2026153838270486E-5
82-83	2.0816999994875815E-4
84-85	9.567813459183307E-4
86-87	0.003614952114494781
88-89	0.0024860301916957463
90-91	0.0037790861529159174
92-93	0.0036709978837117544
94-95	0.0027822721146997485
96-97	0.0020576803841088785
98-99	0.0041273705759071085
100	0.0030104584607974255
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2489792E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.990003738879395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.95484536641486	22.298809269356852
2	13.614727100880556	8.43840887520222
3	5.459289373897903	5.075501943261617
4	2.721657583304448	3.3737671473021718
5	1.5783223036469154	2.4456107045587325
6	0.9315034125555108	1.7320376542724516
7	0.6329051208857485	1.3729612443823682
8	0.4685105068756215	1.1615313887823835
9	0.3497564627077151	0.975505867830839
>10	1.92079708921858	10.97943795933564
>50	0.16243492361444958	3.546275309883097
>100	0.14865179965191502	9.824028897032425
>500	0.028744490188786255	6.268172008402371
>1k	0.024870059280858833	14.95638899527668
>5k	0.0024041055392825168	4.857816751425234
>10k+	5.803013370681937E-4	2.693745983695102
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	41443	0.33181497337986093	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	39909	0.3195329433828842	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20187	0.16162799188329158	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	15363	0.12300445035433737	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	13381	0.10713549112747435	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	13214	0.10579839920472654	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	8.006538459567622E-6
6	0.0	0.0	0.0	0.0	8.006538459567622E-6
7	0.0	0.0	0.0	0.0	8.006538459567622E-6
8	0.0	0.0	0.0	0.0	8.006538459567622E-6
9	8.006538459567622E-6	0.0	0.0	0.0	8.006538459567622E-6
10-11	8.006538459567622E-6	0.0	0.0	1.6013076919135243E-5	1.2009807689351432E-5
12-13	1.6013076919135243E-5	0.0	0.0	1.6013076919135243E-5	2.4019615378702865E-5
14-15	2.8022884608486675E-5	0.0	0.0	3.2026153838270486E-5	2.4019615378702865E-5
16-17	3.2026153838270486E-5	0.0	0.0	7.606211536589241E-5	2.4019615378702865E-5
18-19	3.2026153838270486E-5	0.0	0.0	1.4411769227221717E-4	3.2026153838270486E-5
20-21	3.2026153838270486E-5	0.0	0.0	4.083334614379487E-4	3.2026153838270486E-5
22-23	4.003269229783811E-5	0.0	0.0	0.0016933828841985518	3.6029423068054294E-5
24-25	4.803923075740573E-5	0.0	0.0	0.005196243460259386	4.003269229783811E-5
26-27	5.604576921697335E-5	0.0	0.0	0.008623041920954328	4.003269229783811E-5
28-29	5.604576921697335E-5	0.0	0.0	0.021649679994670847	4.803923075740573E-5
30-31	5.604576921697335E-5	0.0	0.0	0.0609297576773096	4.803923075740573E-5
32-33	6.805557690632478E-5	0.0	0.0	0.12717986016100188	5.2042499987189537E-5
34-35	7.606211536589241E-5	0.0	4.003269229783811E-6	0.21236942937080136	5.604576921697335E-5
36-37	8.406865382546002E-5	0.0	8.006538459567622E-6	0.34058613626231726	5.604576921697335E-5
38-39	9.207519228502764E-5	0.0	8.006538459567622E-6	0.5381634858290674	5.604576921697335E-5
40-41	9.607846151481146E-5	0.0	8.006538459567622E-6	0.7836959974993979	6.0049038446757165E-5
42-43	1.1609480766373051E-4	0.0	8.006538459567622E-6	1.000176784369187	6.405230767654097E-5
44-45	1.2810461535308194E-4	0.0	8.006538459567622E-6	1.251574085461151	8.006538459567622E-5
46-47	1.3611115381264956E-4	0.0	8.006538459567622E-6	1.5248692692400323	8.006538459567622E-5
48-49	1.4411769227221717E-4	0.0	8.006538459567622E-6	1.8105105353235666	8.006538459567622E-5
50-51	1.5212423073178482E-4	0.0	8.006538459567622E-6	2.1223612050544958	8.006538459567622E-5
52-53	1.5212423073178482E-4	0.0	8.006538459567622E-6	2.4373424313231156	8.006538459567622E-5
54-55	1.5212423073178482E-4	0.0	8.006538459567622E-6	2.7435645045169688	8.006538459567622E-5
56-57	1.5212423073178482E-4	4.003269229783811E-6	8.006538459567622E-6	3.0886062794320353	8.006538459567622E-5
58-59	1.5212423073178482E-4	8.006538459567622E-6	8.006538459567622E-6	3.4651417733778116	8.006538459567622E-5
60-61	1.5212423073178482E-4	8.006538459567622E-6	8.006538459567622E-6	3.8367252232863445	8.006538459567622E-5
62-63	1.5212423073178482E-4	1.6013076919135243E-5	8.006538459567622E-6	4.218024607615563	9.607846151481146E-5
64-65	1.5212423073178482E-4	1.6013076919135243E-5	8.006538459567622E-6	4.615140908671657	1.0408499997437907E-4
66-67	1.5212423073178482E-4	1.6013076919135243E-5	8.006538459567622E-6	5.054235490871266	1.0408499997437907E-4
68-69	1.5212423073178482E-4	1.6013076919135243E-5	8.006538459567622E-6	5.524343399794008	1.0408499997437907E-4
70-71	1.6013076919135243E-4	1.6013076919135243E-5	8.006538459567622E-6	6.0619103985078375	1.0408499997437907E-4
72-73	1.6813730765092005E-4	1.6013076919135243E-5	8.006538459567622E-6	6.5738804937664295	1.0408499997437907E-4
74-75	1.6813730765092005E-4	2.4019615378702865E-5	8.006538459567622E-6	7.069853525182805	1.0408499997437907E-4
76-77	1.6813730765092005E-4	2.4019615378702865E-5	8.006538459567622E-6	7.609850508319115	1.0408499997437907E-4
78-79	1.6813730765092005E-4	2.4019615378702865E-5	8.006538459567622E-6	8.160940550491153	1.0408499997437907E-4
80-81	1.6813730765092005E-4	2.4019615378702865E-5	8.006538459567622E-6	8.758968924382408	1.0408499997437907E-4
82-83	1.6813730765092005E-4	3.6029423068054294E-5	8.006538459567622E-6	9.399860301916958	1.0408499997437907E-4
84-85	1.841503845700553E-4	4.003269229783811E-5	8.006538459567622E-6	10.022993177148187	1.0408499997437907E-4
86-87	1.841503845700553E-4	4.003269229783811E-5	8.006538459567622E-6	10.667723689874098	1.1209153843394669E-4
88	1.841503845700553E-4	4.003269229783811E-5	8.006538459567622E-6	11.172179648788386	1.2009807689351432E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACATGA	3760	0.0	26.126648	2
GTACTAG	4340	0.0	24.263193	1
GTACAAG	5415	0.0	22.74534	1
GTACATA	2925	0.0	22.500479	1
GTATAAG	4935	0.0	21.909391	1
CATGGGT	10410	0.0	19.864937	4
TAGTACT	4965	0.0	18.837318	4
TAGGCAT	6700	0.0	18.797949	5
CATGGGG	31425	0.0	18.649895	4
AGGGCTA	5940	0.0	18.354898	5
ATGGGTA	7620	0.0	18.19355	5
GGGCTAT	6320	0.0	17.844868	6
CTAGGCA	7330	0.0	17.824858	4
TTTAGGG	6570	0.0	17.670826	2
GTACATG	124985	0.0	17.666573	1
ATTTAGG	6480	0.0	17.483633	1
CTGTGCG	6065	0.0	17.197205	9
TACATGG	123430	0.0	17.086794	2
GTACAAA	5045	0.0	17.052166	1
GTCCTAC	4350	0.0	16.966803	1
>>END_MODULE