FastQCFastQC Report
Fri 27 May 2016
ERR765482_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765482_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14448744
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA244900.16949570149488427No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT223070.15438712181487885No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT167490.11592011042620728No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA645850.021.1941591
GTATAAG58050.019.8186051
GTACATG1009300.019.3498631
CATGGGG368100.019.0964094
TACATGG1002250.019.012172
GTACTAG57050.018.3551711
GTGATCG42100.018.180318
ACATGGG977800.018.0757083
TAGTACT63550.017.8072644
TAGGCAT83600.017.749115
CAACGCA718750.017.7307745
CATGGGT130750.017.5615524
ATCAACG730600.017.488183
ATGGGTA100100.017.3096395
AACGCAG748050.017.2622666
GGGTACC88250.017.2395557
TATTCGC9450.016.8946959
CTAGGCA89400.016.5975994
TATCAAC832200.016.4928932
GTACAAG49250.016.3995321
Produced by FastQC (version 0.11.5)