##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765482_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	14448744
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.25213610262595	33.0	33.0	33.0	33.0	33.0
2	32.23544219483714	33.0	33.0	33.0	33.0	33.0
3	32.21826360824166	33.0	33.0	33.0	33.0	33.0
4	36.019833350220615	37.0	37.0	37.0	33.0	37.0
5	36.07389451982816	37.0	37.0	37.0	33.0	37.0
6	36.070117790169164	37.0	37.0	37.0	33.0	37.0
7	36.025191601429164	37.0	37.0	37.0	33.0	37.0
8	35.981786513762025	37.0	37.0	37.0	33.0	37.0
9	35.984685174019276	37.0	37.0	37.0	33.0	37.0
10-11	35.959422978218726	37.0	37.0	37.0	33.0	37.0
12-13	35.93892711366469	37.0	37.0	37.0	33.0	37.0
14-15	37.83272909396139	40.0	37.0	40.0	33.0	40.0
16-17	37.87635375088658	40.0	37.0	40.0	33.0	40.0
18-19	37.91528239409598	40.0	37.0	40.0	33.0	40.0
20-21	37.9293108452887	40.0	37.0	40.0	33.0	40.0
22-23	37.91620967192719	40.0	37.0	40.0	33.0	40.0
24-25	37.91177541106687	40.0	37.0	40.0	33.0	40.0
26-27	37.89029472042691	40.0	37.0	40.0	33.0	40.0
28-29	37.84141299755882	40.0	37.0	40.0	33.0	40.0
30-31	37.77925707590916	40.0	37.0	40.0	33.0	40.0
32-33	37.70431952424377	40.0	37.0	40.0	33.0	40.0
34-35	37.60170610677302	40.0	37.0	40.0	33.0	40.0
36-37	37.500943057749524	40.0	37.0	40.0	33.0	40.0
38-39	37.37735923620766	40.0	37.0	40.0	33.0	40.0
40-41	37.2663752987803	40.0	37.0	40.0	33.0	40.0
42-43	37.110435446845756	40.0	37.0	40.0	33.0	40.0
44-45	36.947072942810806	40.0	37.0	40.0	33.0	40.0
46-47	36.74798293886306	37.0	37.0	40.0	33.0	40.0
48-49	36.55842525135749	37.0	37.0	40.0	33.0	40.0
50-51	36.33451551221338	37.0	37.0	40.0	33.0	40.0
52-53	36.183912490940386	37.0	37.0	40.0	33.0	40.0
54-55	36.000819206153835	37.0	37.0	40.0	33.0	40.0
56-57	35.814846432326576	37.0	37.0	40.0	33.0	40.0
58-59	35.61374178959777	37.0	37.0	40.0	30.0	40.0
60-61	35.45345616200273	37.0	33.0	40.0	27.0	40.0
62-63	35.251086668848174	37.0	33.0	37.0	27.0	40.0
64-65	35.059844786508776	37.0	33.0	37.0	27.0	40.0
66-67	34.88371200292565	37.0	33.0	37.0	27.0	40.0
68-69	34.66935797326051	37.0	33.0	37.0	27.0	40.0
70-71	34.49451917758388	37.0	33.0	37.0	27.0	40.0
72-73	34.309595041617456	37.0	33.0	37.0	27.0	37.0
74-75	34.11274575146463	37.0	33.0	37.0	27.0	37.0
76-77	33.94476499133765	37.0	33.0	37.0	27.0	37.0
78-79	33.802359983677476	37.0	33.0	37.0	27.0	37.0
80-81	33.65261627585069	37.0	33.0	37.0	27.0	37.0
82-83	33.533056333477845	37.0	33.0	37.0	27.0	37.0
84-85	33.399462922175104	37.0	33.0	37.0	27.0	37.0
86-87	33.29928556419853	37.0	33.0	37.0	27.0	37.0
88-89	33.22730771615858	37.0	33.0	37.0	27.0	37.0
90-91	33.11822418612995	37.0	33.0	37.0	27.0	37.0
92-93	33.04717385815681	37.0	33.0	37.0	27.0	37.0
94-95	32.95756492744283	37.0	33.0	37.0	27.0	37.0
96-97	32.88461017788121	37.0	33.0	37.0	27.0	37.0
98-99	32.78895037520216	37.0	33.0	37.0	27.0	37.0
100	32.71721680445027	37.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	8.0
4	30.0
5	67.0
6	423.0
7	1090.0
8	1996.0
9	3033.0
10	3179.0
11	3726.0
12	5178.0
13	8685.0
14	15776.0
15	23579.0
16	28269.0
17	35261.0
18	43516.0
19	52051.0
20	61858.0
21	75467.0
22	90126.0
23	91738.0
24	84696.0
25	84345.0
26	93401.0
27	107841.0
28	132155.0
29	168980.0
30	219703.0
31	288431.0
32	392890.0
33	542691.0
34	755965.0
35	1142893.0
36	2280605.0
37	4935998.0
38	2669290.0
39	3804.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.91453012222187	19.450919241129597	12.700764562546398	24.933786074102134
2	17.036855498037788	21.22027767346078	36.40758939243583	25.3352774360656
3	18.242803850865474	25.957767826863314	29.469952314049014	26.32947600822219
4	13.4471480704482	17.398017433210804	36.49669479921577	32.658139697125236
5	14.883035827744001	36.74780715536079	32.787681864981735	15.581475151913477
6	31.480035241900488	36.61883309997917	17.57866965286174	14.322462005258602
7	27.638679182079773	31.70116378281739	22.23038210103245	18.429774934070394
8	27.294794620210588	31.461412839759635	20.42859227071917	20.815200269310605
9	27.46902429719843	14.22801871221471	20.117485644426946	38.185471346159915
10-11	25.897302907436103	24.715255526708756	26.76685599800232	22.620585567852817
12-13	26.60258220368497	23.102810874080127	26.50632470199486	23.788282220240042
14-15	24.704964867780866	24.334876286127553	24.110658929040206	26.84949991705138
16-17	24.21786285700799	26.291314665226945	25.090742564439118	24.400079913325946
18-19	24.457333115213935	25.335757425665996	27.070346516717542	23.136562942402534
20-21	25.95891104384459	24.68475294206417	25.659530996872633	23.69680501721861
22-23	25.057913591830648	24.394753856720712	25.777175739873936	24.770156811574708
24-25	24.502986733793247	25.17799227503851	25.94095156274886	24.378069428419384
26-27	24.677072930072885	25.175602103545536	25.77721496394771	24.37011000243387
28-29	24.09000053698365	24.99994027744709	26.445780006142595	24.464279179426665
30-31	24.743000916161115	24.850854634777438	26.098879203488934	24.307265245572516
32-33	24.30077413259885	24.97748833454949	25.521483604450907	25.200253928400752
34-35	24.899941178427827	24.89368128070029	26.195712309365256	24.010665231506636
36-37	24.755135253757775	24.99488765053731	25.60238642326182	24.647590672443094
38-39	24.909322627649676	24.477835108768872	25.877183900670993	24.73565836291046
40-41	24.644840850965302	24.87922944477153	26.201686844541612	24.27424285972156
42-43	25.22810883469198	24.805919793811128	25.766971910463838	24.198999461033058
44-45	24.6031655356533	25.177403746151132	25.37361386120789	24.845816856987675
46-47	24.34451337676119	24.991413224510158	25.727457667395903	24.93661573133275
48-49	25.29905873567227	25.191201997502915	25.106186405772167	24.403552861052646
50-51	24.4380347670016	25.615202851128288	24.909853309132433	25.03690907273768
52-53	24.446105446119155	25.238826976938178	24.999688432366142	25.31537914457652
54-55	24.81526702142379	25.118041160081	25.13938420401457	24.92730761448064
56-57	24.804438900361998	24.759321200184832	24.70325297164208	25.73298692781109
58-59	24.437771346101	24.835808810320092	25.380364470633992	25.346055372944914
60-61	24.488819317148923	25.050846464760752	25.235616490145723	25.2247177279446
62-63	24.200802708209906	25.041958803265874	24.958465241564493	25.798773246959726
64-65	24.19912161574848	24.79795199449678	25.159066212454366	25.84386017730037
66-67	24.744952242359524	24.939454186427	24.96736232662085	25.348231244592622
68-69	24.81804026717024	24.486820781367687	24.996603307549396	25.698535643912674
70-71	24.04764813713176	24.67900942686668	25.335255974547792	25.938086461453764
72-73	24.869539059183012	24.768638210675004	25.10190386829857	25.25991886184341
74-75	24.999564657681596	24.638131867351976	25.17595878736306	25.186344687603373
76-77	24.430218888718283	24.9352968985615	25.240401601517526	25.394082611202695
78-79	24.535154316179398	24.923338699347422	25.230316248636857	25.31119073583632
80-81	24.150018857679818	24.698201830657514	25.61906932893142	25.532709982731244
82-83	24.43365244375152	24.836548008577513	25.200919942911646	25.528879604759318
84-85	24.45577730761177	24.86285065091268	24.877881285610133	25.803490755865415
86-87	24.10961305331096	24.64229621059014	25.0922327721103	26.1558579639886
88-89	24.21631844298107	24.24574764929801	25.302073391774528	26.235860515946392
90-91	24.539818968330135	24.383019093822647	25.12011799397369	25.957043943873533
92-93	24.185749279009272	24.55901768470172	25.07224097616846	26.18299206012055
94-95	23.49909040396779	24.682326674729968	25.562728831013526	26.255854090288715
96-97	24.29162992926518	24.50648726989552	25.01490233403275	26.18698046680655
98-99	23.711308451548977	24.536348606624923	25.24492260219428	26.50742033963182
100	23.71685473235964	24.714786816083777	24.976340814640103	26.59201763691648
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	524.0
1	406.5
2	414.5
3	540.5
4	473.5
5	484.0
6	716.5
7	1337.0
8	2142.5
9	2270.0
10	1727.0
11	1434.5
12	1579.0
13	1868.0
14	2280.5
15	2796.0
16	3316.0
17	3968.5
18	4854.5
19	5855.5
20	7161.0
21	8753.5
22	11055.0
23	14073.0
24	17860.0
25	23421.0
26	32308.0
27	42454.0
28	51961.5
29	67122.0
30	86701.0
31	106239.5
32	130495.0
33	156124.0
34	184008.0
35	206539.5
36	223156.5
37	248468.5
38	258921.0
39	258727.5
40	260428.5
41	261644.5
42	272520.0
43	313405.5
44	370107.0
45	417709.5
46	483235.0
47	548466.0
48	611821.0
49	660320.5
50	681669.0
51	661150.5
52	635321.0
53	651272.5
54	634968.0
55	599380.0
56	576213.0
57	528463.0
58	501983.0
59	475749.0
60	403331.0
61	325002.0
62	272442.0
63	215084.0
64	155190.5
65	128056.5
66	105110.0
67	77995.0
68	61853.0
69	53862.0
70	49997.5
71	46532.5
72	42798.5
73	47451.0
74	37307.5
75	25739.0
76	20247.0
77	15143.5
78	11412.5
79	6670.5
80	4820.5
81	4241.5
82	4200.5
83	4214.0
84	3335.5
85	2370.0
86	1823.5
87	943.5
88	392.0
89	299.5
90	225.5
91	162.0
92	113.5
93	78.0
94	61.0
95	50.5
96	28.5
97	17.0
98	11.5
99	6.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.029587346830977142
2	0.0018133064022727513
3	3.045247393129811E-4
4	0.0
5	0.002685354519396288
6	2.3531457128730357E-4
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	2.8376168890527787E-4
16-17	0.004086860421916258
18-19	0.006917556294166469
20-21	0.02001212008462466
22-23	0.02583269521558414
24-25	0.03314128895909568
26-27	0.032213872707551605
28-29	0.04819450050468054
30-31	0.048177197962674126
32-33	0.058503355032105214
34-35	0.05269316142634959
36-37	0.056897679133909494
38-39	0.0609949210810296
40-41	0.04342938043611265
42-43	0.05002164894055844
44-45	0.047256702727932615
46-47	0.044830886338632615
48-49	0.038141723598950884
50-51	0.03792371156967
52-53	0.03911758696811294
54-55	0.05842722384727697
56-57	0.045114648027537896
58-59	0.04505927989311735
60-61	0.04662689019889895
62-63	0.031258772388797255
64-65	0.0334804187824215
66-67	0.03411715232825774
68-69	0.03145948187607172
70-71	0.04469938701938383
72-73	0.03364652318568313
74-75	0.042124768768828626
76-77	0.04717711103470308
78-79	0.04148457471459111
80-81	0.03478849095810681
82-83	0.02121291649987016
84-85	0.03397873199220638
86-87	0.027967828899176286
88-89	0.027061175698039914
90-91	0.022565975284772156
92-93	0.015939101696313535
94-95	0.015371578318502978
96-97	0.01509819815480155
98-99	0.01127433637138287
100	0.006000521567826241
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.4448744E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.931541586197604
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.80930690899457	21.49363256022598
2	14.713332776867718	8.807854637647607
3	5.257016531517806	4.720518267973604
4	2.4739959268626377	2.9620204787589004
5	1.3287434725809582	1.9885670253472778
6	0.8456353540775516	1.5186701860398701
7	0.5654450459647886	1.184724933560314
8	0.41306065442542617	0.9890833724445313
9	0.30398245725969597	0.8188797204848811
>10	1.8803934913048164	10.670068847027503
>50	0.1846029568784594	3.8639392643054693
>100	0.16264194092641523	10.333244611910692
>500	0.030375962405472138	6.451600979494966
>1k	0.028329166614323314	16.74347967017782
>5k	0.002649320581116233	5.539775685538642
>10k+	4.880327386266746E-4	1.9139397590619303
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	24490	0.16949570149488427	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	22307	0.15438712181487885	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16749	0.11592011042620728	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.921016802567753E-6
2	0.0	0.0	0.0	0.0	6.921016802567753E-6
3	0.0	0.0	0.0	0.0	6.921016802567753E-6
4	0.0	0.0	0.0	1.3842033605135505E-5	6.921016802567753E-6
5	0.0	5.536813442054202E-5	0.0	1.3842033605135505E-5	6.921016802567753E-6
6	6.921016802567753E-6	5.536813442054202E-5	0.0	1.3842033605135505E-5	6.921016802567753E-6
7	6.921016802567753E-6	6.228915122310977E-5	0.0	1.3842033605135505E-5	1.3842033605135505E-5
8	6.921016802567753E-6	6.228915122310977E-5	0.0	1.3842033605135505E-5	1.3842033605135505E-5
9	6.921016802567753E-6	6.228915122310977E-5	0.0	1.3842033605135505E-5	2.0763050407703257E-5
10-11	6.921016802567753E-6	6.228915122310977E-5	0.0	2.768406721027101E-5	2.0763050407703257E-5
12-13	1.3842033605135505E-5	7.26706764269614E-5	0.0	4.1526100815406514E-5	2.0763050407703257E-5
14-15	2.4223558808987136E-5	8.305220163081303E-5	0.0	5.536813442054202E-5	3.4605084012838764E-5
16-17	2.768406721027101E-5	8.305220163081303E-5	0.0	7.613118482824527E-5	5.536813442054202E-5
18-19	3.1144575611554886E-5	8.305220163081303E-5	0.0	1.0727576043980018E-4	5.536813442054202E-5
20-21	4.498660921669039E-5	8.997321843338079E-5	0.0	2.872221973065617E-4	5.882864282182589E-5
22-23	4.844711761797427E-5	9.689423523594854E-5	0.0	0.0011281257388185437	6.921016802567752E-5
24-25	6.574965962439365E-5	9.689423523594854E-5	0.0	0.0030037212923144044	7.959169322952915E-5
26-27	1.314993192487873E-4	9.689423523594854E-5	0.0	0.00503503972386804	8.305220163081303E-5
28-29	1.4534135285392281E-4	9.689423523594854E-5	0.0	0.016762702695819094	9.343372683466466E-5
30-31	1.591833864590583E-4	9.689423523594854E-5	0.0	0.055080912223235454	1.0035474363723242E-4
32-33	1.6956491166290994E-4	1.0035474363723242E-4	0.0	0.12100705777609459	1.0381525203851629E-4
34-35	1.7302542006419381E-4	1.1419677724236791E-4	0.0	0.20748170221577739	1.176572856436518E-4
36-37	1.7302542006419381E-4	1.2803881084750342E-4	0.0	0.34092236667768494	1.2111779404493566E-4
38-39	1.7302542006419381E-4	1.3842033605135506E-4	0.0	0.5319908775461728	1.3495982765007118E-4
40-41	1.8686745366932933E-4	1.6956491166290994E-4	0.0	0.7502486029235482	1.4534135285392281E-4
42-43	2.0070948727446482E-4	1.7302542006419381E-4	0.0	0.950629341899891	1.5226236965649054E-4
44-45	2.0763050407703257E-4	1.7302542006419381E-4	0.0	1.1931556126954703	1.6610440326162606E-4
46-47	2.180120292808842E-4	1.764859284654777E-4	0.0	1.4630129788443895	1.6956491166290994E-4
48-49	2.2147253768216809E-4	1.7994643686676157E-4	0.0	1.7291710615123361	1.8686745366932933E-4
50-51	2.2839355448473584E-4	1.8686745366932933E-4	0.0	2.0019525572603403	1.8686745366932933E-4
52-53	2.3185406288601972E-4	1.903279620706132E-4	0.0	2.2755403514658434	1.9724897887318094E-4
54-55	2.353145712873036E-4	2.2839355448473584E-4	0.0	2.562686417587577	2.1109101247831645E-4
56-57	2.3877507968858748E-4	2.2839355448473584E-4	0.0	2.883960017562772	2.2493304608345196E-4
58-59	2.4569609649115524E-4	2.5261711329372294E-4	0.0	3.229547149565388	2.3185406288601972E-4
60-61	2.5607762169500684E-4	2.5607762169500684E-4	0.0	3.5778473201546097	2.353145712873036E-4
62-63	2.5607762169500684E-4	2.5607762169500684E-4	0.0	3.9272894585162557	2.4223558808987133E-4
64-65	2.629986384975746E-4	2.629986384975746E-4	0.0	4.29273644823384	2.491566048924391E-4
66-67	2.629986384975746E-4	2.629986384975746E-4	0.0	4.696788869676146	2.5953813009629075E-4
68-69	2.629986384975746E-4	2.6991965530014236E-4	0.0	5.14118389806062	2.6991965530014236E-4
70-71	2.629986384975746E-4	2.768406721027101E-4	0.0	5.618606018626948	2.768406721027101E-4
72-73	2.629986384975746E-4	2.8376168890527787E-4	0.0	6.079483448526737	2.9068270570784563E-4
74-75	2.629986384975746E-4	2.9068270570784563E-4	0.0	6.538772505070337	2.9068270570784563E-4
76-77	2.629986384975746E-4	2.9068270570784563E-4	0.0	7.059471743703121	2.9068270570784563E-4
78-79	2.629986384975746E-4	2.9068270570784563E-4	0.0	7.593798464420161	2.9068270570784563E-4
80-81	2.6645914689885845E-4	2.9068270570784563E-4	0.0	8.16192743120094	3.114457561155489E-4
82-83	2.768406721027101E-4	2.9068270570784563E-4	0.0	8.76105563224042	3.5989287373352314E-4
84-85	2.8376168890527787E-4	3.045247393129811E-4	0.0	9.349674269265204	3.668138905360909E-4
86-87	2.8376168890527787E-4	3.183667729181166E-4	0.0	9.971475721349897	3.7719541573994256E-4
88	2.8376168890527787E-4	3.3912982332581987E-4	0.0	10.454964113143676	3.806559241412264E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	64585	0.0	21.194159	1
GTATAAG	5805	0.0	19.818605	1
GTACATG	100930	0.0	19.349863	1
CATGGGG	36810	0.0	19.096409	4
TACATGG	100225	0.0	19.01217	2
GTACTAG	5705	0.0	18.355171	1
GTGATCG	4210	0.0	18.18031	8
ACATGGG	97780	0.0	18.075708	3
TAGTACT	6355	0.0	17.807264	4
TAGGCAT	8360	0.0	17.74911	5
CAACGCA	71875	0.0	17.730774	5
CATGGGT	13075	0.0	17.561552	4
ATCAACG	73060	0.0	17.48818	3
ATGGGTA	10010	0.0	17.309639	5
AACGCAG	74805	0.0	17.262266	6
GGGTACC	8825	0.0	17.239555	7
TATTCGC	945	0.0	16.894695	9
CTAGGCA	8940	0.0	16.597599	4
TATCAAC	83220	0.0	16.492893	2
GTACAAG	4925	0.0	16.399532	1
>>END_MODULE