FastQCFastQC Report
Fri 27 May 2016
ERR765481_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765481_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12315534
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT616790.5008227820247177No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA613020.49776160741385633No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG244050.1981643670505883No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA190240.15447158036346617No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC181610.14746417004735646No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC175220.14227560087934474No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT171830.1395229796775357No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169850.13791525401984192No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC167480.1359908551265418No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG155910.12659621580355346No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG155430.12620646412896105No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG51800.021.6887021
TAGTACT56750.020.1239914
GTACATA28650.019.1967031
TAGGCAT73000.018.9259455
TACATGA41300.018.8916782
GTATAAG57000.018.8853951
CTAGTAC69700.018.2062513
GTACAAG48900.018.168471
CGTTAAC13950.017.859411
CATGGGG290050.017.7263014
CTAGGCA79450.017.2727954
ACGTTAA11750.016.7940858
GTATATA30400.016.5453381
CATGGGT79750.016.3827884
CTAACGC25600.016.3395333
TCTAACG24750.016.3318632
CTGTGCG80950.015.902799
CCTAGTA78650.015.5974742
GTACATG1507150.015.5043191
TACTGTG95600.015.4327447
Produced by FastQC (version 0.11.5)