##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765481_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	12315534
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69454146283872	33.0	33.0	33.0	27.0	33.0
2	31.574880796886273	33.0	33.0	33.0	27.0	33.0
3	31.564166198558667	33.0	33.0	33.0	27.0	33.0
4	35.15560827488276	37.0	37.0	37.0	33.0	37.0
5	35.377771114106785	37.0	37.0	37.0	33.0	37.0
6	35.365253021103264	37.0	37.0	37.0	33.0	37.0
7	35.233215628327606	37.0	37.0	37.0	33.0	37.0
8	35.20421591138476	37.0	37.0	37.0	33.0	37.0
9	34.901647220494056	37.0	37.0	37.0	33.0	37.0
10-11	34.92467971750149	37.0	37.0	37.0	33.0	37.0
12-13	34.8961855409599	37.0	37.0	37.0	33.0	37.0
14-15	36.19579573244652	37.0	37.0	40.0	30.0	40.0
16-17	36.44119008562682	37.0	37.0	40.0	33.0	40.0
18-19	36.258851260529994	37.0	37.0	40.0	33.0	40.0
20-21	36.25831332202079	37.0	37.0	40.0	30.0	40.0
22-23	36.456536517214765	37.0	37.0	40.0	33.0	40.0
24-25	36.47902758418758	37.0	37.0	40.0	33.0	40.0
26-27	36.14356080702631	37.0	37.0	40.0	27.0	40.0
28-29	36.02781868817057	37.0	37.0	40.0	27.0	40.0
30-31	35.8247350054005	37.0	37.0	40.0	27.0	40.0
32-33	35.772800919554115	37.0	37.0	40.0	27.0	40.0
34-35	35.7209082854223	37.0	37.0	40.0	27.0	40.0
36-37	35.48826051716475	37.0	37.0	40.0	27.0	40.0
38-39	35.342951267886555	37.0	33.0	40.0	27.0	40.0
40-41	35.47237196535693	37.0	33.0	40.0	27.0	40.0
42-43	35.3832171223757	37.0	33.0	40.0	27.0	40.0
44-45	35.31245254976358	37.0	33.0	40.0	27.0	40.0
46-47	35.2581973708976	37.0	33.0	40.0	27.0	40.0
48-49	35.07718276771433	37.0	33.0	40.0	27.0	40.0
50-51	34.920974315851836	37.0	33.0	40.0	27.0	40.0
52-53	34.80409095537392	37.0	33.0	40.0	27.0	40.0
54-55	34.672337228738925	37.0	33.0	40.0	27.0	40.0
56-57	34.451700876307925	37.0	33.0	40.0	27.0	40.0
58-59	34.250790505714164	37.0	33.0	38.5	27.0	40.0
60-61	33.962189662259064	37.0	33.0	37.0	27.0	40.0
62-63	33.71585657593086	37.0	33.0	37.0	27.0	40.0
64-65	33.514300435531254	37.0	33.0	37.0	27.0	40.0
66-67	33.2891030953266	37.0	33.0	37.0	24.5	40.0
68-69	33.07840467981332	37.0	33.0	37.0	22.0	38.5
70-71	32.84287347994817	37.0	33.0	37.0	22.0	37.0
72-73	32.598411810645	37.0	33.0	37.0	22.0	37.0
74-75	32.37258274793444	33.0	33.0	37.0	22.0	37.0
76-77	32.1694849772653	33.0	33.0	37.0	22.0	37.0
78-79	31.980422002001703	33.0	33.0	37.0	22.0	37.0
80-81	31.851315095228514	33.0	33.0	37.0	22.0	37.0
82-83	31.719053798235628	33.0	33.0	37.0	22.0	37.0
84-85	31.557682557654424	33.0	33.0	37.0	22.0	37.0
86-87	31.346267242654683	33.0	33.0	37.0	22.0	37.0
88-89	31.21135539067977	33.0	27.0	37.0	22.0	37.0
90-91	31.116688850032812	33.0	27.0	37.0	22.0	37.0
92-93	31.013101624338823	33.0	27.0	37.0	15.0	37.0
94-95	30.89282031944372	33.0	27.0	37.0	15.0	37.0
96-97	30.794432178093132	33.0	27.0	37.0	15.0	37.0
98-99	30.665602806991558	33.0	27.0	37.0	15.0	37.0
100	30.564868969546914	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	151.0
8	1014.0
9	2359.0
10	3842.0
11	7216.0
12	13337.0
13	23054.0
14	33651.0
15	39440.0
16	45048.0
17	53148.0
18	63440.0
19	74619.0
20	86553.0
21	102141.0
22	117486.0
23	122020.0
24	126298.0
25	137826.0
26	160196.0
27	192148.0
28	235707.0
29	293599.0
30	363485.0
31	453486.0
32	581277.0
33	767770.0
34	1009500.0
35	1371888.0
36	2038860.0
37	2808864.0
38	985135.0
39	971.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.83284798774794	18.250177384157627	12.583567963519387	25.333406664575048
2	15.68655288186411	20.90460721079633	37.10546744352601	26.303372463813552
3	18.213691067472624	26.124528144664698	28.93729313754083	26.724487650321848
4	12.742954951077037	17.35876501749207	36.01269418426871	33.88558584716218
5	14.12094871694295	37.36036068739935	33.206197473216406	15.312493122441296
6	32.23360344342653	35.64703578599341	17.394986041293212	14.724374729286854
7	27.56194917983977	31.482925565123153	22.139212160378765	18.815913094658306
8	27.56610463804754	32.503702457385806	20.05856533207133	19.871627572495328
9	27.267910548778257	14.220562861026838	19.43427418506807	39.077252405126835
10-11	25.417438995103858	25.14639002980235	26.54032249715344	22.89584847794035
12-13	26.891748706963508	22.789179003736056	26.130640107163465	24.18843218213697
14-15	24.768421379141447	23.360379191467885	24.282300749574347	27.58889867981632
16-17	24.06849478254437	25.87357048098296	25.563059944770156	24.494874791702514
18-19	24.551282139771747	25.272829602398588	27.134453400066473	23.041434857763196
20-21	25.342883375578246	24.75581530895224	26.31591512786085	23.585386187608666
22-23	24.783231601516967	24.674730658184103	25.571727285477756	24.970310454821174
24-25	24.407470531040467	25.280829589378744	25.598094994361176	24.713604885219617
26-27	24.812285066385783	24.785230350680205	25.71502807424233	24.68745650869168
28-29	24.26518280346507	24.76168082690018	26.53024205004671	24.442894319588042
30-31	24.941613280471152	24.75592846408881	26.178691610285032	24.123766645155005
32-33	24.08556699841779	25.118361085221117	25.848213828792243	24.947858087568843
34-35	24.288910024895827	25.197563806216465	26.41440558961092	24.099120579276786
36-37	24.908145289722462	24.88212999354065	25.80544758271189	24.404277134024998
38-39	25.017435518640518	24.538665052445076	25.891167348441584	24.552732080472826
40-41	24.46858395533483	24.872623604416475	26.0940811042938	24.564711335954897
42-43	25.32768552069285	24.685764424873664	25.51758526558666	24.468964788846822
44-45	25.06715353700345	24.875441233013074	25.421346426131926	24.63605880385155
46-47	24.497410098491248	24.91836405224993	26.126281961232195	24.457943888026623
48-49	25.274291211667776	25.130632902156634	25.43000297416007	24.16507291201552
50-51	24.099823229494707	25.652476318170013	25.14698723780795	25.10071321452733
52-53	24.020079411307727	25.64164972426415	24.49872418908188	25.83954667534624
54-55	24.41535624845825	25.41096472146478	24.494808751289227	25.67887027878775
56-57	24.939738707229424	24.66798841203313	24.515611746920595	25.876661133816853
58-59	24.387984313144685	24.765560307819374	25.499422111944153	25.34703326709179
60-61	24.592831297449223	25.076902877293016	24.979233543588123	25.35103228166964
62-63	24.00370688413742	25.111423933705463	24.970239652755282	25.914629529401832
64-65	23.80152576673014	25.036581910960592	25.26694584301929	25.89494647928998
66-67	24.97665033749981	24.794225580252053	24.90035616532791	25.328767916920224
68-69	24.846542487789353	24.4553892959612	24.92487949721728	25.773188719032163
70-71	24.062362115060978	24.768142186746125	25.078685255890896	26.090810442301997
72-73	25.058638544795393	24.662328742624684	24.978450920939697	25.300581791640226
74-75	25.28569828964688	24.525162423012837	24.976417980326787	25.212721307013496
76-77	24.574352152864666	24.8363914121051	25.44540340599476	25.143853029035473
78-79	24.6808461573814	25.024444737840845	25.22983169061122	25.06487741416653
80-81	24.062579853451687	24.97650324236986	25.497434986349347	25.463481917829107
82-83	24.2237531554725	25.02065891064673	24.902779089357978	25.85280884452279
84-85	24.469593514313743	25.144335945841966	24.502954276795347	25.883116263048944
86-87	24.32798903876307	24.537273893733218	25.010719621672163	26.124017445831555
88-89	24.44277433235395	24.08567114741383	25.29713667848728	26.17441784174494
90-91	24.680937966718343	24.473910666568955	25.004241763680472	25.840909603032223
92-93	24.271383664077355	24.566522838440402	25.04419901279229	26.11789448468996
94-95	23.559662808027866	24.80124022542747	25.60154112235708	26.037555844187587
96-97	24.48963500926759	24.505964382883455	25.00157427016896	26.002826337679995
98-99	24.294340783976022	24.600917008423437	24.78874417430062	26.31599803329992
100	24.02658508709783	24.792737415424465	24.94524032342212	26.235437174055587
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	771.0
1	757.5
2	1005.5
3	1313.5
4	1157.0
5	1038.5
6	1311.5
7	2417.0
8	3923.0
9	3874.5
10	2625.5
11	1914.0
12	1775.5
13	1895.5
14	2227.0
15	2612.5
16	3129.5
17	3837.0
18	4800.5
19	6016.0
20	7455.5
21	9292.5
22	11810.0
23	15007.0
24	18778.5
25	23594.5
26	30201.5
27	37631.5
28	45556.5
29	57590.5
30	70581.0
31	81514.5
32	95488.0
33	110754.0
34	125848.0
35	138920.0
36	152349.5
37	171278.0
38	184222.0
39	195693.5
40	206375.0
41	212993.0
42	226570.5
43	255670.0
44	297249.0
45	344345.5
46	400830.5
47	459153.0
48	529381.0
49	603305.5
50	694314.5
51	696933.0
52	604159.5
53	565611.5
54	555177.5
55	529427.0
56	500498.0
57	465993.0
58	436020.5
59	403902.0
60	347243.0
61	283971.0
62	229702.5
63	182595.0
64	138255.0
65	104430.5
66	80863.0
67	61587.5
68	48160.0
69	39903.5
70	33976.0
71	29365.5
72	24746.5
73	23598.5
74	19242.5
75	13972.0
76	10385.0
77	7269.0
78	5187.5
79	3325.5
80	2320.5
81	1728.5
82	1254.0
83	942.0
84	712.5
85	480.0
86	325.0
87	190.0
88	102.0
89	64.5
90	43.5
91	25.0
92	16.5
93	14.5
94	11.0
95	9.5
96	8.0
97	4.5
98	2.5
99	3.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.049465983367022494
2	0.0247817106428353
3	0.02194789117548618
4	0.022808592790211125
5	0.015711864382007307
6	0.02555309416546615
7	0.04911683082520011
8	0.006008671649966619
9	0.003954355531802356
10-11	0.0018797398472530707
12-13	0.0011611351972232791
14-15	0.0022573117820144867
16-17	0.00661765864151729
18-19	0.004977453677607484
20-21	0.008391840743568245
22-23	0.01848884506347837
24-25	0.02541099720077099
26-27	0.02799310204494584
28-29	0.043964800876681434
30-31	0.04187800545230113
32-33	0.03978715011464383
34-35	0.04741978707541224
36-37	0.04440327151059792
38-39	0.05365987378216811
40-41	0.051528419311740764
42-43	0.056302877325498026
44-45	0.056729168219583494
46-47	0.06119913273756542
48-49	0.0638015371481253
50-51	0.033218210432450594
52-53	2.720141895592997E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.643279455036217E-4
64-65	1.7051635763418784E-4
66-67	3.69452108207407E-4
68-69	0.001254513202594382
70-71	9.743791864810734E-5
72-73	3.4509262854538015E-4
74-75	5.683878587806261E-5
76-77	1.055577452021163E-4
78-79	0.0
80-81	4.059913277004473E-5
82-83	2.0299566385022363E-4
84-85	0.0013641308610735027
86-87	0.0040639731902814774
88-89	0.002647063456606916
90-91	0.003990894751295397
92-93	0.004031493884065441
94-95	0.002870358686842162
96-97	0.002464367359141715
98-99	0.003970595184910374
100	0.0029150177328892113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.2315534E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.379408812367096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.86374886308838	24.706332009533963
2	13.585336735818625	9.341116909887546
3	5.603604797063461	5.779458604235583
4	2.742099894882683	3.770870931620824
5	1.5893731454615336	2.732085456160993
6	0.9784491094610829	2.0183101161755457
7	0.651114639078714	1.5669455452422776
8	0.4654974779727323	1.280282247708033
9	0.33512059763492896	1.0369123224782406
>10	1.8089792134198786	11.512981038525934
>50	0.17619652275723496	4.245446331052662
>100	0.15745680261235914	11.309312370615684
>500	0.025070279407238175	6.0215485366580195
>1k	0.016879793240189914	10.432414352819643
>5k	7.230631379215426E-4	1.5345913159255984
>10k+	3.490649631345378E-4	2.7113919113595673
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	61679	0.5008227820247177	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	61302	0.49776160741385633	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	24405	0.1981643670505883	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	19024	0.15447158036346617	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	18161	0.14746417004735646	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	17522	0.14227560087934474	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	17183	0.1395229796775357	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16985	0.13791525401984192	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	16748	0.1359908551265418	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	15591	0.12659621580355346	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	15543	0.12620646412896105	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	8.119826554008946E-6	0.0
3	0.0	0.0	0.0	8.119826554008946E-6	0.0
4	8.119826554008946E-6	0.0	0.0	2.4359479662026835E-5	0.0
5	8.119826554008946E-6	2.4359479662026835E-5	0.0	6.495861243207157E-5	0.0
6	8.119826554008946E-6	2.4359479662026835E-5	0.0	6.495861243207157E-5	0.0
7	8.119826554008946E-6	2.4359479662026835E-5	0.0	7.307843898608051E-5	0.0
8	8.119826554008946E-6	2.4359479662026835E-5	0.0	7.307843898608051E-5	0.0
9	8.119826554008946E-6	2.4359479662026835E-5	0.0	9.743791864810734E-5	0.0
10-11	2.4359479662026835E-5	2.4359479662026835E-5	0.0	1.3803705141815208E-4	0.0
12-13	2.4359479662026835E-5	3.2479306216035776E-5	0.0	1.786361841881968E-4	4.059913277004473E-6
14-15	2.4359479662026835E-5	4.059913277004472E-5	0.0	2.070555771272281E-4	2.029956638502236E-5
16-17	2.4359479662026835E-5	4.059913277004472E-5	0.0	3.2073314888335337E-4	3.247930621603578E-5
18-19	3.2479306216035776E-5	4.059913277004472E-5	0.0	3.9381158786943384E-4	3.247930621603578E-5
20-21	4.059913277004472E-5	4.871895932405367E-5	0.0	5.277887260105815E-4	3.653921949304025E-5
22-23	4.46590460470492E-5	4.871895932405367E-5	0.0	0.001571186438200731	4.871895932405367E-5
24-25	5.683878587806262E-5	4.871895932405367E-5	0.0	0.0040152542309574235	4.871895932405367E-5
26-27	6.495861243207157E-5	4.871895932405367E-5	0.0	0.007129207714419853	4.871895932405367E-5
28-29	8.525817881709392E-5	5.683878587806262E-5	0.0	0.018233070527027086	5.2778872601058144E-5
30-31	1.0149783192511181E-4	5.683878587806262E-5	0.0	0.0514634606993087	5.683878587806262E-5
32-33	1.1367757175612524E-4	5.683878587806262E-5	0.0	0.11116042552438246	6.089869915506709E-5
34-35	1.2179739831013418E-4	6.089869915506709E-5	0.0	0.18894024408523413	6.495861243207157E-5
36-37	1.2179739831013418E-4	6.495861243207157E-5	0.0	0.30768458761106093	6.901852570907604E-5
38-39	1.2991722486414313E-4	6.495861243207157E-5	0.0	0.495622033116875	8.525817881709392E-5
40-41	1.2991722486414313E-4	6.495861243207157E-5	1.623965310801789E-5	0.7269640114671438	8.93180920940984E-5
42-43	1.2991722486414313E-4	6.495861243207157E-5	1.623965310801789E-5	0.9265006292053597	8.93180920940984E-5
44-45	1.339771381411476E-4	6.495861243207157E-5	1.623965310801789E-5	1.1514076450115764	8.93180920940984E-5
46-47	1.3803705141815208E-4	7.713835226308497E-5	1.623965310801789E-5	1.4006416611736041	1.0149783192511181E-4
48-49	1.4209696469515655E-4	8.93180920940984E-5	1.623965310801789E-5	1.660277175151317	1.1367757175612524E-4
50-51	1.4615687797216103E-4	8.93180920940984E-5	1.623965310801789E-5	1.9418808798709013	1.1367757175612524E-4
52-53	1.4615687797216103E-4	9.743791864810734E-5	1.623965310801789E-5	2.226732515212089	1.3397713814114758E-4
54-55	1.5427670452616995E-4	1.1773748503312971E-4	1.623965310801789E-5	2.505437441851892	1.5427670452616995E-4
56-57	1.5427670452616995E-4	1.2179739831013418E-4	2.4359479662026835E-5	2.8223299127751993	1.5427670452616995E-4
58-59	1.5427670452616995E-4	1.3803705141815208E-4	2.4359479662026835E-5	3.164600901593061	1.5427670452616995E-4
60-61	1.5427670452616995E-4	1.4615687797216103E-4	2.4359479662026835E-5	3.504801334639651	1.5427670452616995E-4
62-63	1.5427670452616995E-4	1.4615687797216103E-4	2.4359479662026835E-5	3.8488383857330097	1.623965310801789E-4
64-65	1.5427670452616995E-4	1.5427670452616995E-4	2.4359479662026835E-5	4.220917257830639	1.6645644435718337E-4
66-67	1.5427670452616995E-4	1.623965310801789E-4	2.4359479662026835E-5	4.625012606030725	1.7051635763418784E-4
68-69	1.5427670452616995E-4	1.623965310801789E-4	2.4359479662026835E-5	5.068663689288666	1.7051635763418784E-4
70-71	1.5427670452616995E-4	1.7051635763418784E-4	2.4359479662026835E-5	5.582859825647836	1.7051635763418784E-4
72-73	1.5427670452616995E-4	1.786361841881968E-4	2.4359479662026835E-5	6.056834401171724	1.908159240192102E-4
74-75	1.5427670452616995E-4	1.8269609746520126E-4	2.4359479662026835E-5	6.527699083125425	1.9487583729621468E-4
76-77	1.5427670452616995E-4	1.9487583729621468E-4	2.4359479662026835E-5	7.039244096114711	1.9487583729621468E-4
78-79	1.5427670452616995E-4	2.0299566385022363E-4	2.4359479662026835E-5	7.562006649488362	1.9487583729621468E-4
80-81	1.5427670452616995E-4	2.2735514351225047E-4	2.4359479662026835E-5	8.130414807835372	1.9487583729621468E-4
82-83	1.5427670452616995E-4	2.3141505678925495E-4	2.4359479662026835E-5	8.741756549086706	1.9487583729621468E-4
84-85	1.5427670452616995E-4	2.3547497006625942E-4	3.247930621603578E-5	9.337102232026641	1.9487583729621468E-4
86-87	1.5427670452616995E-4	2.3547497006625942E-4	3.247930621603578E-5	9.951180354826676	1.9487583729621468E-4
88	1.5427670452616995E-4	2.3547497006625942E-4	3.247930621603578E-5	10.43544681050777	1.9487583729621468E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	5180	0.0	21.688702	1
TAGTACT	5675	0.0	20.123991	4
GTACATA	2865	0.0	19.196703	1
TAGGCAT	7300	0.0	18.925945	5
TACATGA	4130	0.0	18.891678	2
GTATAAG	5700	0.0	18.885395	1
CTAGTAC	6970	0.0	18.206251	3
GTACAAG	4890	0.0	18.16847	1
CGTTAAC	1395	0.0	17.85941	1
CATGGGG	29005	0.0	17.726301	4
CTAGGCA	7945	0.0	17.272795	4
ACGTTAA	1175	0.0	16.794085	8
GTATATA	3040	0.0	16.545338	1
CATGGGT	7975	0.0	16.382788	4
CTAACGC	2560	0.0	16.339533	3
TCTAACG	2475	0.0	16.331863	2
CTGTGCG	8095	0.0	15.90279	9
CCTAGTA	7865	0.0	15.597474	2
GTACATG	150715	0.0	15.504319	1
TACTGTG	9560	0.0	15.432744	7
>>END_MODULE