##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765479_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7640413
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.216942722860665	33.0	31.0	34.0	31.0	34.0
2	32.23274854382872	34.0	31.0	34.0	30.0	34.0
3	32.250860653736915	34.0	31.0	34.0	30.0	34.0
4	35.71939265586821	37.0	35.0	37.0	35.0	37.0
5	35.74801597767032	37.0	35.0	37.0	35.0	37.0
6	35.84885712329949	37.0	37.0	37.0	35.0	37.0
7	35.808702618562634	37.0	36.0	37.0	35.0	37.0
8	35.83930227331952	37.0	37.0	37.0	35.0	37.0
9	37.531412634369374	39.0	38.0	39.0	35.0	39.0
10-11	37.46382741351809	39.0	38.0	39.0	35.0	39.0
12-13	37.446643002151845	39.0	38.0	39.0	35.0	39.0
14-15	38.83288926920574	41.0	39.0	41.0	35.0	41.0
16-17	38.786503098719926	40.5	38.5	41.0	35.0	41.0
18-19	38.760338413643346	41.0	38.5	41.0	35.0	41.0
20-21	38.73749998854774	40.5	39.0	41.0	35.0	41.0
22-23	38.64720820720032	40.0	38.5	41.0	34.5	41.0
24-25	38.56651034440154	40.0	38.0	41.0	34.0	41.0
26-27	38.444948787454294	40.0	38.0	41.0	34.0	41.0
28-29	38.31385711740975	40.0	38.0	41.0	34.0	41.0
30-31	38.11655678822598	40.0	38.0	41.0	34.0	41.0
32-33	37.878990834657756	40.0	38.0	41.0	33.0	41.0
34-35	37.74342787490676	40.0	37.5	41.0	33.0	41.0
36-37	37.61068092785037	40.0	37.0	41.0	33.0	41.0
38-39	37.43740796472652	40.0	37.0	41.0	32.5	41.0
40-41	37.214687478281604	40.0	37.0	41.0	32.0	41.0
42-43	36.99120531835125	40.0	36.0	41.0	31.0	41.0
44-45	36.70602956934396	40.0	36.0	41.0	31.0	41.0
46-47	36.33478465103915	39.0	35.0	41.0	30.0	41.0
48-49	35.98355232891207	39.0	35.0	41.0	29.5	41.0
50-51	34.738560664194466	38.0	34.0	40.0	26.5	40.5
52-53	34.695886204057295	38.0	34.0	39.5	26.5	40.0
54-55	35.55133230363333	39.0	35.0	40.5	28.0	41.0
56-57	35.590735081990985	39.0	35.0	41.0	28.0	41.0
58-59	35.39937134288421	38.0	35.0	41.0	28.0	41.0
60-61	35.08159696341022	38.0	35.0	40.0	28.0	41.0
62-63	34.66102630839458	37.0	35.0	40.0	27.5	41.0
64-65	34.38279337779254	36.5	34.0	40.0	26.5	41.0
66-67	34.112822173356335	36.0	34.0	39.0	26.0	41.0
68-69	33.90650158571271	36.0	34.0	39.0	26.0	41.0
70-71	33.56126180613535	35.0	34.0	38.5	26.0	41.0
72-73	33.20328155035598	35.0	34.0	37.0	26.0	39.5
74-75	32.84389606949258	35.0	34.0	37.0	26.0	39.0
76-77	32.48941621349526	35.0	34.0	36.5	25.0	39.0
78-79	32.18063421440699	35.0	33.5	36.0	24.5	38.0
80-81	31.879407958705897	35.0	33.0	35.5	24.0	37.0
82-83	31.62392281673779	35.0	33.0	35.0	23.0	37.0
84-85	31.36925641323316	35.0	33.0	35.0	21.0	36.0
86-87	31.113436341726555	35.0	33.0	35.0	20.0	36.0
88-89	30.900442685493573	35.0	33.0	35.0	18.5	36.0
90-91	30.66291822182911	35.0	33.0	35.0	14.5	35.5
92-93	30.386495141034914	35.0	32.0	35.0	4.5	35.0
94-95	30.10564572883691	35.0	32.0	35.0	2.0	35.0
96-97	29.84676044344723	35.0	32.0	35.0	2.0	35.0
98-99	29.533094676950054	35.0	31.5	35.0	2.0	35.0
100	28.96809661990785	34.0	31.0	35.0	2.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.4971526006288798
1101	2	0.5604462471596037
1101	3	0.626364854436197
1101	4	0.44510365629101045
1101	5	0.49326168019519656
1101	6	0.46461099233793846
1101	7	0.540403044581538
1101	8	0.5345527516524626
1101	9	0.613060620678489
1101	10-11	0.6038565539987459
1101	12-13	0.5602129153389797
1101	14-15	0.7561157418317279
1101	16-17	0.7401458942578429
1101	18-19	0.8069072171165885
1101	20-21	0.7393509426890219
1101	22-23	0.7327773404492746
1101	24-25	0.7562594705993178
1101	26-27	0.7719840968987555
1101	28-29	0.8015360040582848
1101	30-31	0.802537201831754
1101	32-33	0.8217099945819157
1101	34-35	0.8958880937164366
1101	36-37	0.9702149788308603
1101	38-39	0.8767383998459337
1101	40-41	0.9430837992623324
1101	42-43	0.9040514385473699
1101	44-45	0.9838907684171403
1101	46-47	0.9719458014985136
1101	48-49	1.0292667609181407
1101	50-51	0.9606869472989104
1101	52-53	1.0468194120503753
1101	54-55	1.1578698348588006
1101	56-57	1.1489657406357026
1101	58-59	1.1572982865923862
1101	60-61	1.1422105046610014
1101	62-63	1.2172609569159292
1101	64-65	1.1641498251573736
1101	66-67	1.1535390702831592
1101	68-69	1.0843025605959937
1101	70-71	1.0885845689761737
1101	72-73	1.1151666887861609
1101	74-75	1.0038902507402057
1101	76-77	0.9616146215459338
1101	78-79	0.9285692173593247
1101	80-81	0.9988857730013905
1101	82-83	0.9928220594229558
1101	84-85	1.0242054212086664
1101	86-87	1.030230326336433
1101	88-89	1.0753163864564712
1101	90-91	1.0830956655010873
1101	92-93	1.0877026422294058
1101	94-95	0.9269580742003782
1101	96-97	1.0081484098749378
1101	98-99	1.0979057119292506
1101	100	1.1235663948133023
1102	1	0.47952253349978946
1102	2	0.5686284069247449
1102	3	0.549375586845315
1102	4	0.4255676452134125
1102	5	0.45155701682376304
1102	6	0.4252661656736336
1102	7	0.4589894025386201
1102	8	0.41754066299112935
1102	9	0.5244625031797412
1102	10-11	0.481056990243637
1102	12-13	0.5667547579850094
1102	14-15	0.7454643680567656
1102	16-17	0.7606463089170106
1102	18-19	0.6852209575793253
1102	20-21	0.7341617304913868
1102	22-23	0.7501669092129575
1102	24-25	0.7337593543735892
1102	26-27	0.7471825136474806
1102	28-29	0.775481224270159
1102	30-31	0.835987875960754
1102	32-33	0.8689375334819758
1102	34-35	0.8362259951709703
1102	36-37	0.8250902366840691
1102	38-39	0.8629721369687431
1102	40-41	0.9275518666872173
1102	42-43	0.9666249883630371
1102	44-45	0.9960803123684343
1102	46-47	1.015359029211524
1102	48-49	1.027887645490118
1102	50-51	1.0985056148765935
1102	52-53	1.0834745448223018
1102	54-55	1.1508784455721397
1102	56-57	1.1592883371698974
1102	58-59	1.1653978174355402
1102	60-61	1.1810273306699628
1102	62-63	1.244968999986746
1102	64-65	1.2655352490414984
1102	66-67	1.2647755632398798
1102	68-69	1.2376552021072413
1102	70-71	1.1294859643829653
1102	72-73	1.0764795746440683
1102	74-75	1.1209952541360977
1102	76-77	1.0700835855881365
1102	78-79	1.0979197216341277
1102	80-81	1.000820256458013
1102	82-83	0.9966011407141906
1102	84-85	1.0969854735280258
1102	86-87	1.1186200277225673
1102	88-89	1.0922771514153808
1102	90-91	1.1290751836957185
1102	92-93	1.1993320242522536
1102	94-95	1.0466816795344087
1102	96-97	1.1153504936936436
1102	98-99	1.246706242629763
1102	100	1.2290250004759251
1103	1	0.42522820818484774
1103	2	0.4992105596264267
1103	3	0.5292310053100096
1103	4	0.47083880630695063
1103	5	0.4561848651469873
1103	6	0.4193788463071826
1103	7	0.423756074801247
1103	8	0.4459306557457694
1103	9	0.5344370299199284
1103	10-11	0.5366671380615244
1103	12-13	0.5305374225763941
1103	14-15	0.6623712141468658
1103	16-17	0.7204714895949493
1103	18-19	0.6601773378515148
1103	20-21	0.6548695939690177
1103	22-23	0.709219443051083
1103	24-25	0.6776836519058662
1103	26-27	0.6754908322959992
1103	28-29	0.7112684175499098
1103	30-31	0.721278198020002
1103	32-33	0.7407453218610627
1103	34-35	0.7341580117022062
1103	36-37	0.7162033102594592
1103	38-39	0.7304324506389435
1103	40-41	0.7333120095520016
1103	42-43	0.7370095166669159
1103	44-45	0.7804526517609673
1103	46-47	0.742550200375355
1103	48-49	0.760731105616685
1103	50-51	0.6468710980524008
1103	52-53	0.7319910332569677
1103	54-55	0.836360952803112
1103	56-57	0.846787680532131
1103	58-59	0.9250441354945806
1103	60-61	0.9006349202638262
1103	62-63	0.971540537748524
1103	64-65	0.9624820334021464
1103	66-67	0.805513946139115
1103	68-69	0.786117028891276
1103	70-71	0.771153791262762
1103	72-73	0.6962388674614033
1103	74-75	0.7180313412337611
1103	76-77	0.7138761147462134
1103	78-79	0.7286225365009713
1103	80-81	0.7311394601709935
1103	82-83	0.7131127061717066
1103	84-85	0.63694820956405
1103	86-87	0.658311684094258
1103	88-89	0.6556362616843607
1103	90-91	0.7357142354532229
1103	92-93	0.672063770854848
1103	94-95	0.769326847911028
1103	96-97	0.8001456159739853
1103	98-99	0.7937662135870909
1103	100	0.8403586969957608
1104	1	0.3286366818240012
1104	2	0.3933656680481903
1104	3	0.45427496991341343
1104	4	0.3643097526249903
1104	5	0.4700482984266827
1104	6	0.35953474962529697
1104	7	0.47354447912675823
1104	8	0.44290660985566177
1104	9	0.5498450096429082
1104	10-11	0.5439012460604857
1104	12-13	0.5377611010060974
1104	14-15	0.6016665457018817
1104	16-17	0.5651709586901816
1104	18-19	0.6086234918927218
1104	20-21	0.629783106269791
1104	22-23	0.5739330019872426
1104	24-25	0.6294467948444407
1104	26-27	0.6062383270219627
1104	28-29	0.6309663018136646
1104	30-31	0.5787562818677827
1104	32-33	0.6423030982019213
1104	34-35	0.6429280005615254
1104	36-37	0.5962602649685564
1104	38-39	0.6300136141312578
1104	40-41	0.6223206547388287
1104	42-43	0.6535041119781226
1104	44-45	0.7244362510979272
1104	46-47	0.8011824494634467
1104	48-49	0.7421102562903386
1104	50-51	0.6888500371579411
1104	52-53	0.8003325057335431
1104	54-55	0.9458216305156455
1104	56-57	0.9244408620744338
1104	58-59	0.9216595748953154
1104	60-61	0.9057347150350381
1104	62-63	0.8276472545124065
1104	64-65	0.9123526505162332
1104	66-67	0.8279050957786254
1104	68-69	0.794833701263336
1104	70-71	0.8048645625492483
1104	72-73	0.8226321603443267
1104	74-75	0.7829537451282036
1104	76-77	0.7454622416551118
1104	78-79	0.7411647660584393
1104	80-81	0.794496690006838
1104	82-83	0.7574195670312207
1104	84-85	0.7781494511899361
1104	86-87	0.7697345871417092
1104	88-89	0.7768359046976272
1104	90-91	0.7953239424495813
1104	92-93	0.7795771178921491
1104	94-95	0.8356564608869128
1104	96-97	0.868125239726723
1104	98-99	0.897228014738424
1104	100	0.8956160102350843
1105	1	0.2587057372244246
1105	2	0.2914161104537101
1105	3	0.3699836735698554
1105	4	0.3308143897682214
1105	5	0.35345086886344035
1105	6	0.3062327022454099
1105	7	0.3383173049470898
1105	8	0.35506011255271375
1105	9	0.39879084315175106
1105	10-11	0.4119744591559851
1105	12-13	0.4153129173340915
1105	14-15	0.5234149085165285
1105	16-17	0.449740409294634
1105	18-19	0.4740138271099923
1105	20-21	0.4707033493884083
1105	22-23	0.4157382543603205
1105	24-25	0.43132578559988133
1105	26-27	0.4324330449156619
1105	28-29	0.39167707440657296
1105	30-31	0.4203093965463296
1105	32-33	0.441982078550744
1105	34-35	0.48131804587990956
1105	36-37	0.4468164496019327
1105	38-39	0.41470546648601214
1105	40-41	0.5058609890888732
1105	42-43	0.4644783952217253
1105	44-45	0.47673604123095004
1105	46-47	0.42240418741963737
1105	48-49	0.4646320296786115
1105	50-51	0.49897548656315394
1105	52-53	0.5591449114234948
1105	54-55	0.5538258625437678
1105	56-57	0.5098707920279253
1105	58-59	0.5197438140594173
1105	60-61	0.4965682114881105
1105	62-63	0.5197482780784597
1105	64-65	0.47870326958662446
1105	66-67	0.4463787409969058
1105	68-69	0.4129745822090598
1105	70-71	0.39615892389210217
1105	72-73	0.3320298892219711
1105	74-75	0.3614010268925725
1105	76-77	0.41278241143179173
1105	78-79	0.37912898212973545
1105	80-81	0.34479880428646226
1105	82-83	0.3752073660049291
1105	84-85	0.4213662385652128
1105	86-87	0.3943885296426224
1105	88-89	0.46136149880450006
1105	90-91	0.49083724930383
1105	92-93	0.47022161018505315
1105	94-95	0.46434311983945875
1105	96-97	0.4464673154522778
1105	98-99	0.4630663304506939
1105	100	0.5107056187229304
1106	1	0.18274504156474336
1106	2	0.2724284132207089
1106	3	0.3348008097274615
1106	4	0.3018284155651543
1106	5	0.33140252977263174
1106	6	0.30797478642278264
1106	7	0.2730081006989735
1106	8	0.31236517152968446
1106	9	0.30935696079126984
1106	10-11	0.33124994219922854
1106	12-13	0.3471106338055847
1106	14-15	0.38043401489080253
1106	16-17	0.3988921850848328
1106	18-19	0.3860295027011418
1106	20-21	0.4177152442204388
1106	22-23	0.4265856304801616
1106	24-25	0.4598738083698066
1106	26-27	0.4650570380287249
1106	28-29	0.4700146903164466
1106	30-31	0.44277768830519904
1106	32-33	0.4178710754260493
1106	34-35	0.4502363636138469
1106	36-37	0.46815171724699667
1106	38-39	0.4583550827484544
1106	40-41	0.49151013112459196
1106	42-43	0.4252363746973913
1106	44-45	0.4228256937834942
1106	46-47	0.44028379401329687
1106	48-49	0.4516635583487201
1106	50-51	0.4056331594024485
1106	52-53	0.4441214977521497
1106	54-55	0.5186919953396583
1106	56-57	0.5307279535874869
1106	58-59	0.5249740663171067
1106	60-61	0.5106361444197489
1106	62-63	0.515216579744056
1106	64-65	0.47846239722425565
1106	66-67	0.5026728785935717
1106	68-69	0.5017121141987531
1106	70-71	0.5093861830218245
1106	72-73	0.47199951723175104
1106	74-75	0.4854352575069285
1106	76-77	0.5007392284686958
1106	78-79	0.4424499801982975
1106	80-81	0.5117172036814708
1106	82-83	0.44995109886548335
1106	84-85	0.5483471281171433
1106	86-87	0.5906493232307604
1106	88-89	0.5477126111957702
1106	90-91	0.5970062149331312
1106	92-93	0.5716887317464341
1106	94-95	0.6100750376773156
1106	96-97	0.6162750166090767
1106	98-99	0.6209762645931747
1106	100	0.6830902541845134
1107	1	0.21204375404271758
1107	2	0.23333190750688715
1107	3	0.25326205910696586
1107	4	0.2540282492879484
1107	5	0.26763153541953955
1107	6	0.22248794588326604
1107	7	0.26041333077187545
1107	8	0.22877474988152358
1107	9	0.2887029671301562
1107	10-11	0.35479351257962577
1107	12-13	0.3617333549477806
1107	14-15	0.3583208949721737
1107	16-17	0.3767651513096766
1107	18-19	0.387051309822219
1107	20-21	0.42502934780896595
1107	22-23	0.4303349510201713
1107	24-25	0.43692834830217464
1107	26-27	0.4251655078039889
1107	28-29	0.40553785264715714
1107	30-31	0.4303833839224893
1107	32-33	0.4767313795113992
1107	34-35	0.4452188944356905
1107	36-37	0.5030689245001199
1107	38-39	0.564522419532949
1107	40-41	0.5299062795570819
1107	42-43	0.5374151335654886
1107	44-45	0.5175082948636884
1107	46-47	0.5361316076958857
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2311	2	-0.17665026978490772
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2311	4	-0.07769730003899156
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2311	22-23	-0.21537017024749616
2311	24-25	-0.29780094594026707
2311	26-27	-0.3608460011550605
2311	28-29	-0.301447484838981
2311	30-31	-0.27023969158244654
2311	32-33	-0.2993278964302917
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2311	38-39	-0.3176059389343209
2311	40-41	-0.252388554026453
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2311	46-47	-0.1700203140160923
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2311	80-81	-0.20037515053837396
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2313	26-27	-0.49397363376045433
2313	28-29	-0.54484926326991
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2313	34-35	-0.45809328262388505
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2313	100	-0.2809146079354683
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2314	4	-0.37292394007072005
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2314	24-25	-0.5428572223283012
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2314	80-81	-0.46430597801155926
2314	82-83	-0.5095319005025303
2314	84-85	-0.559682412961191
2314	86-87	-0.6677548576186894
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2314	90-91	-0.5903667766666878
2314	92-93	-0.596844810424944
2314	94-95	-0.536814670610049
2314	96-97	-0.47901773663448566
2314	98-99	-0.5666529598671843
2314	100	-0.531484760621943
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2315	3	-0.5517056735833563
2315	4	-0.39875242171576986
2315	5	-0.4027200706258611
2315	6	-0.42699144921297716
2315	7	-0.4591710207047228
2315	8	-0.48369919299161523
2315	9	-0.5767553064173043
2315	10-11	-0.5744006845362222
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2315	16-17	-0.7866248051606348
2315	18-19	-0.8194000222575966
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2315	22-23	-0.7220136074709274
2315	24-25	-0.8067969218534401
2315	26-27	-0.7189349684358248
2315	28-29	-0.7637207860361102
2315	30-31	-0.7723189270119946
2315	32-33	-0.8044792013622128
2315	34-35	-0.8208156600905738
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2315	54-55	-0.979229329349117
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2315	62-63	-0.7588598545625658
2315	64-65	-0.8065733299144782
2315	66-67	-0.7348229849060175
2315	68-69	-0.7124185545318937
2315	70-71	-0.6340387715858213
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2315	80-81	-0.5394606931605068
2315	82-83	-0.46780681803103974
2315	84-85	-0.45501397413067224
2315	86-87	-0.4448908237498692
2315	88-89	-0.43763952388162153
2315	90-91	-0.38258423138125863
2315	92-93	-0.3782667799760979
2315	94-95	-0.34937013521146554
2315	96-97	-0.38777182146045064
2315	98-99	-0.7251051996638616
2315	100	-1.0155167339145876
2316	1	-0.6352762478671217
2316	2	-0.7183851148398368
2316	3	-0.7284483737587237
2316	4	-0.617545071371012
2316	5	-0.6132105047640906
2316	6	-0.6056466829924929
2316	7	-0.6601843858874901
2316	8	-0.6483937021031565
2316	9	-0.8372661260606762
2316	10-11	-0.8396106236286656
2316	12-13	-0.8725347813169364
2316	14-15	-1.0438118086031807
2316	16-17	-1.0557906625983904
2316	18-19	-1.048798742725431
2316	20-21	-1.0664794520916345
2316	22-23	-1.1031534955496909
2316	24-25	-1.0953404271069331
2316	26-27	-1.1078438246265137
2316	28-29	-1.0487445979018943
2316	30-31	-1.217209773543047
2316	32-33	-1.1687682545268956
2316	34-35	-1.0713245619573541
2316	36-37	-1.1311233337142212
2316	38-39	-1.0161694515595485
2316	40-41	-1.122619181774411
2316	42-43	-0.9993143596928391
2316	44-45	-0.9379986353496577
2316	46-47	-0.9278274102896731
2316	48-49	-0.8879646751415322
2316	50-51	-0.8477592650772223
2316	52-53	-0.9812409795613206
2316	54-55	-1.0500665398742868
2316	56-57	-1.0530999946607977
2316	58-59	-1.113912494172311
2316	60-61	-0.9767954788699456
2316	62-63	-0.9636158870893823
2316	64-65	-0.9448883010329396
2316	66-67	-0.9007675314051653
2316	68-69	-0.9402528874983673
2316	70-71	-0.9567474166096304
2316	72-73	-0.9465437509313546
2316	74-75	-0.849302408057639
2316	76-77	-0.8470377209576867
2316	78-79	-0.7947100795324147
2316	80-81	-0.6801023172881528
2316	82-83	-0.6630500835330295
2316	84-85	-0.6486364382815282
2316	86-87	-0.6846576315345452
2316	88-89	-0.7001326436918873
2316	90-91	-0.6630737616770723
2316	92-93	-0.5537618135516844
2316	94-95	-0.4711081328338338
2316	96-97	-0.5069691629603703
2316	98-99	-0.6732659075978873
2316	100	-0.8342678800678698
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	20963.0
3	3581.0
4	3559.0
5	4082.0
6	4988.0
7	5653.0
8	6855.0
9	8233.0
10	10414.0
11	14194.0
12	19812.0
13	26590.0
14	27519.0
15	32529.0
16	41513.0
17	56092.0
18	55982.0
19	35614.0
20	30920.0
21	30515.0
22	32740.0
23	37801.0
24	43280.0
25	49612.0
26	57431.0
27	67881.0
28	81840.0
29	99800.0
30	122751.0
31	153351.0
32	194698.0
33	262075.0
34	381707.0
35	610043.0
36	1094583.0
37	1874180.0
38	1652910.0
39	383568.0
40	554.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.466628849659315	18.250624026483962	13.044941306982505	24.23780581687422
2	15.180303122863096	21.770180766221763	37.17476826990849	25.874747841006652
3	18.985799196896906	26.952502030619456	29.31684995016409	24.744848822319547
4	13.389156796197632	18.073574190646063	35.28763189675922	33.24963711639708
5	13.870411415214456	36.6970198175555	33.57257697542085	15.859991791809191
6	31.198182134652264	34.41121745704366	18.443228013811456	15.947372394492614
7	25.956818598315124	31.940541349732754	22.614497987956312	19.48814206399581
8	27.32280275945943	31.29957748398528	21.495974040951552	19.88164571560374
9	27.74289508632138	14.660331513452363	20.0476120210516	37.54916137917466
10-11	25.081055351896108	25.554415459061392	26.45604844945201	22.90848073959049
12-13	26.97122093356924	23.48587610456946	26.58387513056425	22.95902783129705
14-15	25.516709580648957	23.35493943377139	24.96940310075233	26.158947884827327
16-17	23.42947282524571	26.283518968584413	27.023851511721166	23.263156694448714
18-19	24.5292965669885	25.34724894101805	28.319850892405228	21.803603599588218
20-21	24.67751807523003	24.890600827947985	27.523456537974532	22.908424558847454
22-23	23.4787125457703	25.524362001731472	25.98991311386314	25.007012338635093
24-25	23.442001994452482	26.3034532375893	25.670377489894157	24.584167278064058
26-27	24.27595221831369	24.999455893979302	25.893146755227026	24.83144513247998
28-29	23.65556862390301	24.535977510204457	27.821740310519672	23.98671355537286
30-31	24.903123343671695	24.703516208921826	27.023728607231757	23.36963184017472
32-33	22.678700622884538	25.468507933413903	27.158221041601255	24.6945704021003
34-35	22.745638135796863	25.797250762950668	27.71279411796507	23.744316983287398
36-37	24.187013824090908	25.531457347853614	26.532049167898396	23.749479660157082
38-39	24.65879253401857	24.58492341662811	26.277009583204737	24.47927446614858
40-41	23.67490737909766	25.615859245047623	26.47026267107129	24.238970704783434
42-43	25.33697843445426	24.873918385903853	26.132501255151098	23.65660192449079
44-45	25.076435150900178	24.672610985974632	26.314621381273067	23.93633248185212
46-47	23.896886833371063	24.93007378446223	27.777495234373905	23.395544147792805
48-49	24.479062779685208	25.605540099705266	26.999432963606363	22.915964157003163
50-51	23.23698373737111	26.0502727921251	26.28766491291481	24.42507855758898
52-53	23.040754795105702	26.21309819254849	25.066118604669445	25.680028407676364
54-55	23.8328683584745	25.90923511772142	24.88217381012328	25.375722713680805
56-57	24.375889253640924	24.669815722904882	25.1459923211055	25.808302702348694
58-59	23.999639401091336	24.551885098931464	26.750981557567155	24.69749394241005
60-61	24.307272366192695	25.036332487256445	26.07272208994265	24.583673056608202
62-63	22.537240095897573	25.405514038471832	26.57554226679893	25.48170359883167
64-65	22.587474072711906	25.46115540138924	26.668342906527855	25.283027619371
66-67	24.140022590247987	25.262276552483414	25.690779832602622	24.90692102466598
68-69	24.62902588951908	24.493016241244593	25.545368296363215	25.33258957287311
70-71	23.416547693528692	25.07132490950222	25.954593979430644	25.557533417538448
72-73	24.71286934810611	24.67636867527857	25.813998103066126	24.796763873549192
74-75	25.03716945349592	24.319897686792487	25.9538979435722	24.68903491613939
76-77	23.941687981350835	25.188963388009565	26.85366278916131	24.01568584147829
78-79	24.28665571435763	25.249468290391054	26.487416992474266	23.976459002777055
80-81	23.343070789276297	25.284238756035364	26.418481956448503	24.954208498239836
82-83	23.43296646004884	25.81109746852257	25.078984777348218	25.67695129408038
84-85	23.881883252187453	25.768370961791735	24.725046166496195	25.62469961952462
86-87	24.036277774896885	24.619813085925706	25.372441276232	25.971467862945413
88-89	24.18841281087657	24.203187623138458	26.252953569529748	25.355445996455224
90-91	24.74987755969088	24.829222813825822	25.600934414610748	24.819965211872546
92-93	23.610128112038222	25.14167633361275	25.952690965967022	25.295504588382006
94-95	23.143205826468623	25.416613967296577	26.362582435933657	25.07759777030114
96-97	24.66789768219888	24.972234509903636	25.360097654669804	24.99977015322768
98-99	24.853061275706352	24.50222156437997	25.212638638776347	25.43207852113733
100	24.455968448167212	25.086668730458143	25.535030933220927	24.922331888153714
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2257.0
1	2523.5
2	1997.5
3	1148.0
4	942.0
5	917.0
6	1220.0
7	2106.0
8	3174.5
9	3358.0
10	2809.0
11	2478.5
12	2696.5
13	3074.0
14	3466.5
15	3846.5
16	4295.0
17	4877.0
18	5595.0
19	6358.5
20	7306.0
21	8522.0
22	10063.5
23	12197.0
24	14744.5
25	17927.5
26	23087.0
27	29195.0
28	34787.5
29	43270.5
30	51757.0
31	58560.0
32	66895.5
33	76517.0
34	88328.0
35	98288.5
36	107808.5
37	118337.0
38	123974.0
39	128636.0
40	136255.5
41	143054.5
42	148879.0
43	164484.0
44	186786.0
45	205463.5
46	232430.0
47	267519.0
48	351304.0
49	468633.5
50	617782.5
51	575812.0
52	381137.0
53	314271.5
54	286159.0
55	261385.0
56	243542.0
57	222562.0
58	209718.0
59	193939.5
60	166595.5
61	136964.0
62	110019.5
63	86054.5
64	64376.0
65	53457.0
66	44996.0
67	34372.0
68	26282.5
69	21235.5
70	17764.5
71	15106.5
72	13222.0
73	13367.0
74	10858.0
75	8249.5
76	6633.0
77	4862.5
78	3727.0
79	2880.0
80	2241.0
81	1790.0
82	1471.0
83	1272.5
84	949.0
85	604.0
86	444.0
87	278.0
88	181.5
89	151.0
90	131.5
91	104.0
92	97.0
93	87.0
94	74.0
95	53.0
96	37.5
97	35.0
98	25.0
99	18.5
100	12.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.11682614539292575
2	0.3749535529034883
3	0.385254043204209
4	0.3816154964397867
5	0.30928956327360835
6	0.14141905679706057
7	0.15510941620564228
8	0.13000606119067123
9	0.21318219316154768
10-11	0.2133654293295402
12-13	0.22577967971103133
14-15	0.15418669121682296
16-17	0.13959978341484944
18-19	0.15831604914551084
20-21	0.1591013470083358
22-23	0.1673535187168547
24-25	0.18007534409461898
26-27	0.17306656066890624
28-29	0.18118130525143078
30-31	0.22496820525277889
32-33	0.22157833614491781
34-35	0.13523483612731407
36-37	0.09398052173357645
38-39	0.08691938511700872
40-41	0.08647438299474125
42-43	0.07688066077056305
44-45	0.09993569719333234
46-47	0.14789776416536646
48-49	0.22833189776521245
50-51	0.18325580043906003
52-53	0.11351480607134719
54-55	0.06145610191490957
56-57	0.07657308577428995
58-59	0.1134100996896372
60-61	0.08833292127009365
62-63	0.09786774615455998
64-65	0.10799808858500189
66-67	0.103606964702039
68-69	0.09333265099674586
70-71	0.1287757612055788
72-73	0.15426522100310547
74-75	0.19894212524898852
76-77	0.19884396301613538
78-79	0.15953326083288955
80-81	0.09388890364958019
82-83	0.13485527549361534
84-85	0.16474240332296175
86-87	0.17720900689530789
88-89	0.1644740932198299
90-91	0.18648206582549923
92-93	0.10307034449577529
94-95	0.3464472404829425
96-97	0.3487376925828486
98-99	0.38836251391122445
100	0.3903584793125712
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7640413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.05096297454053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.4002470368877	29.048046501585574
2	11.939915059886419	8.847707016060212
3	3.6064227996682123	4.008643128631372
4	1.6364639159125682	2.425302558305927
5	0.9209694110037809	1.7061401773892746
6	0.5988470543618469	1.331271602314407
7	0.44514356224686846	1.1545098350214662
8	0.32441609728901233	0.9615943047200106
9	0.2353274932274712	0.7847199214616227
>10	1.5438793530981076	11.02621982920754
>50	0.16856494076196665	4.373808776214543
>100	0.14854836848017244	11.209274981092907
>500	0.018845428918623473	4.820191859645086
>1k	0.010702730531473355	7.1840387825255325
>5k	8.178166186280958E-4	2.193573882966298
>10k+	8.889311072044517E-4	8.924956842858338
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	99780	1.3059503458778996	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	90598	1.185773596270254	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	42364	0.5544726443452729	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	38375	0.5022634247651272	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	33612	0.43992386275453954	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	31933	0.41794861089315455	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	31246	0.4089569503638089	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	30344	0.39715130582600705	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	27579	0.3609621626474904	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	26957	0.35282124146953836	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	25405	0.3325082034177996	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	22342	0.2924187475205856	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20798	0.2722104158505568	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	19239	0.25180575971482166	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	15478	0.2025806720134108	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	14130	0.18493764669527682	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	13875	0.18160013077827075	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13317	0.1742968606539987	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	12590	0.164781668216103	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12248	0.16030547039800072	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	12183	0.15945473104660704	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	11276	0.14758364502023646	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	10898	0.14263626848443925	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10720	0.1403065514913919	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	10165	0.13304254626026107	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	9793	0.12817369951074634	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9488	0.12418176870805282	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	9001	0.11780776772145694	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	8759	0.11464039967472962	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	8747	0.11448334010216463	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	8697	0.11382892521647717	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	8640	0.11308289224679346	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	8475	0.11092332312402484	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	7800	0.10208872216724411	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.926489314124773E-5	0.0	0.0	0.0	0.0
2	9.161808399624471E-5	0.0	0.0	1.3088297713749243E-5	0.0
3	1.0470638170999395E-4	0.0	0.0	1.3088297713749243E-5	0.0
4	1.177946794237432E-4	1.3088297713749243E-5	0.0	3.926489314124773E-5	0.0
5	7.591212673974561E-4	1.3088297713749243E-5	0.0	3.926489314124773E-5	0.0
6	0.0015313308325086614	1.3088297713749243E-5	0.0	6.544148856874622E-5	0.0
7	0.0017669201913561479	1.3088297713749243E-5	0.0	9.161808399624471E-5	0.0
8	0.0020548627410586313	1.3088297713749243E-5	0.0	9.161808399624471E-5	0.0
9	0.0021726574204823743	1.3088297713749245E-4	0.0	1.177946794237432E-4	0.0
10-11	0.004011563249264143	1.3088297713749245E-4	0.0	1.8323616799248942E-4	0.0
12-13	0.0076566541625433075	1.3088297713749245E-4	0.0	3.010308474162326E-4	6.544148856874622E-6
14-15	0.0116813057095212	1.9632446570623865E-4	0.0	3.5992818712810416E-4	1.9632446570623865E-5
16-17	0.013422049305449848	1.9632446570623865E-4	0.0	4.973553131224713E-4	2.6176595427498486E-5
18-19	0.01452801046226166	2.0286861456311327E-4	0.0	6.413265879737129E-4	2.6176595427498486E-5
20-21	0.017904791272408967	2.094127634199879E-4	0.0	9.554457331036948E-4	2.6176595427498486E-5
22-23	0.01917435615064264	2.2250106113373715E-4	0.0	0.0018650824242092672	3.2720744284373105E-5
24-25	0.026726303931475955	2.4213350770436104E-4	0.0	0.004351858989821624	4.5809041998122355E-5
26-27	0.0514173775684639	2.617659542749849E-4	0.0	0.00855320255593513	5.235319085499697E-5
28-29	0.05683593282195609	2.683101031318595E-4	0.0	0.02730218903088092	6.544148856874622E-5
30-31	0.05807932110476227	3.2066329398685645E-4	0.0	0.07838581500764422	6.544148856874622E-5
32-33	0.05895623705158347	3.2720744284373107E-4	0.0	0.1686165394462315	6.544148856874622E-5
34-35	0.05951248970441782	3.991930802693519E-4	0.0	0.2928310288985687	6.544148856874622E-5
36-37	0.06070352479636899	4.711787176949728E-4	0.0	0.47274931342062265	6.544148856874622E-5
38-39	0.06197963382345954	4.973553131224713E-4	0.0	0.7381210937157454	6.544148856874622E-5
40-41	0.06319684551083822	5.627968016912175E-4	0.0	1.0937955840868812	6.544148856874622E-5
42-43	0.06561163643902496	6.282382902599638E-4	0.0	1.3682113781022047	6.544148856874622E-5
44-45	0.06841907629862418	7.329446719699577E-4	0.0	1.667534202666793	6.544148856874622E-5
46-47	0.06966246458143036	7.722095651112054E-4	0.0	2.0085497995985295	6.544148856874622E-5
48-49	0.0705001156351103	7.852978628249546E-4	0.0	2.3243246143893006	6.544148856874622E-5
50-51	0.0717893129599146	9.358132865330709E-4	0.0	2.65945047734985	6.544148856874622E-5
52-53	0.07239791880360394	9.554457331036948E-4	0.0	3.0459151880925806	6.544148856874622E-5
54-55	0.0741386623995326	9.750781796743187E-4	0.0	3.4056797714992633	7.852978628249547E-5
56-57	0.07761360544253301	9.816223285311933E-4	0.0	3.7930279423376723	9.161808399624471E-5
58-59	0.0790075091490473	9.881664773880678E-4	0.0	4.19564361245917	9.816223285311933E-5
60-61	0.07991060169129602	0.0010012547751018172	0.0	4.566016261162845	1.0470638170999395E-4
62-63	0.08047339849298722	0.0010077989239586917	0.0	4.9393926741918275	1.3088297713749245E-4
64-65	0.08101656284810782	0.0010470638170999396	0.0	5.3468182937231274	1.3088297713749245E-4
66-67	0.08173641922236402	0.0010470638170999396	0.0	5.781251615586749	1.3088297713749245E-4
68-69	0.08250208463861836	0.001073240412527438	0.0	6.275537722895346	1.3088297713749245E-4
70-71	0.08356878090228892	0.0010928728590980617	0.0	6.858804622210867	1.3088297713749245E-4
72-73	0.08618644044503877	0.0010994170079549365	0.0	7.354203234825134	1.3088297713749245E-4
74-75	0.08864704041522363	0.001125593603382435	0.0	7.834975674744284	1.4397127485124168E-4
76-77	0.08939307338490733	0.001158314347666808	0.0	8.354882124827546	1.5705957256499092E-4
78-79	0.08992969359117106	0.0011648584965236826	0.0	8.870665761130976	1.5705957256499092E-4
80-81	0.09083278613341975	0.0011648584965236826	0.0	9.440916348370173	1.5705957256499092E-4
82-83	0.09127778825568722	0.001177946794237432	0.0	10.084526844294935	1.5705957256499092E-4
84-85	0.09296617866076087	0.0011975792408080558	0.0	10.688669578463887	1.5705957256499092E-4
86-87	0.09609428181434695	0.0012172116873786797	0.0	11.294324011018777	1.6360372142186554E-4
88	0.0968010498908894	0.0012957414736611752	0.0	11.772963058410586	1.7014787027874015E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATCTAC	4730	0.0	25.795908	1
TCTACAC	6535	0.0	22.269522	3
TACACTC	6280	0.0	21.12791	5
AGTACTT	21970	0.0	19.812355	12-13
ATCTACA	6740	0.0	18.796215	2
GAGTACT	21325	0.0	18.725332	12-13
CTACACT	8855	0.0	17.599733	4
CATGGGG	15240	0.0	16.652157	4
GTACTAG	1990	0.0	16.571323	1
GTGATCG	1455	0.0	16.481106	8
GTACTTT	24530	0.0	16.393465	14-15
CACTCTT	8150	0.0	16.271717	7
CGTTAAC	555	0.0	16.127714	1
GTATAAG	2675	0.0	16.026173	1
CATGGGT	8120	0.0	15.945691	4
GTCCTAC	1980	0.0	15.94123	1
TACTTTT	27755	0.0	15.935792	14-15
ATGGGTA	5365	0.0	15.873619	5
TAGTACT	2160	0.0	15.694427	4
ATACCGA	965	0.0	15.592204	6
>>END_MODULE