##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765479_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	7640413
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.52840585973559	34.0	31.0	34.0	31.0	34.0
2	32.66606530301438	34.0	31.0	34.0	31.0	34.0
3	32.7138797339882	34.0	31.0	34.0	31.0	34.0
4	36.20394735206068	37.0	35.0	37.0	35.0	37.0
5	36.14676588817908	37.0	35.0	37.0	35.0	37.0
6	36.19695623783688	37.0	37.0	37.0	35.0	37.0
7	36.15633173232913	37.0	37.0	37.0	35.0	37.0
8	36.16347270756175	37.0	37.0	37.0	35.0	37.0
9	37.90581883466247	39.0	38.0	39.0	35.0	39.0
10-11	37.85686735782477	39.0	38.0	39.0	35.0	39.0
12-13	37.82327565276903	39.0	38.0	39.0	35.0	39.0
14-15	39.21501462028296	41.0	39.0	41.0	36.0	41.0
16-17	39.188290672245074	40.0	39.0	41.0	36.0	41.0
18-19	39.208653380386636	41.0	39.0	41.0	36.0	41.0
20-21	39.18033337988405	40.5	39.0	41.0	36.0	41.0
22-23	39.11453713300577	40.0	39.0	41.0	35.5	41.0
24-25	39.05374395337006	40.0	39.0	41.0	35.0	41.0
26-27	38.92472546968338	40.0	38.0	41.0	35.0	41.0
28-29	38.79606200607219	40.0	38.0	41.0	35.0	41.0
30-31	38.645525182997304	40.0	38.0	41.0	35.0	41.0
32-33	38.41167807289999	40.0	38.0	41.0	34.0	41.0
34-35	38.312715097992736	40.0	38.0	41.0	34.0	41.0
36-37	38.2094178678561	40.0	38.0	41.0	34.0	41.0
38-39	38.05639138879011	40.0	38.0	41.0	33.5	41.0
40-41	37.85807848345371	40.0	37.0	41.0	33.0	41.0
42-43	37.63677218757677	40.0	37.0	41.0	33.0	41.0
44-45	37.36469684295862	40.0	36.5	41.0	33.0	41.0
46-47	37.055152515970015	40.0	36.0	41.0	32.0	41.0
48-49	36.78274518668036	39.5	35.5	41.0	31.0	41.0
50-51	36.52377593986084	39.0	35.0	41.0	31.0	41.0
52-53	36.39794929933761	39.0	35.0	41.0	31.0	41.0
54-55	36.39560210946712	39.0	35.0	41.0	31.0	41.0
56-57	36.22990707439506	39.0	35.0	41.0	31.0	41.0
58-59	36.10442936788888	39.0	35.0	41.0	31.0	41.0
60-61	35.802354336081045	38.0	35.0	40.5	30.5	41.0
62-63	35.475654850071585	37.0	35.0	40.0	30.0	41.0
64-65	35.251590653541896	37.0	35.0	40.0	30.0	41.0
66-67	34.91404482977556	36.0	35.0	39.0	30.0	41.0
68-69	34.59254597886266	36.0	35.0	39.0	30.0	41.0
70-71	34.22172256918572	35.0	34.0	38.5	29.0	40.5
72-73	33.861642426921165	35.0	34.0	37.0	29.0	39.5
74-75	33.525976409913966	35.0	34.0	37.0	29.0	39.0
76-77	32.07305606385414	34.5	32.0	35.5	26.5	37.5
78-79	32.86358957297204	35.0	33.5	36.0	28.5	37.0
80-81	32.82784327234667	35.0	34.0	35.5	29.0	37.0
82-83	32.66678463585673	35.0	34.0	35.0	29.0	37.0
84-85	32.48758372093236	35.0	34.0	35.0	29.0	36.0
86-87	32.31611785907385	35.0	34.0	35.0	28.0	36.0
88-89	32.1687215075939	35.0	34.0	35.0	27.0	36.0
90-91	32.04044820613755	35.0	34.0	35.0	27.0	35.5
92-93	31.887321797918517	35.0	33.0	35.0	27.0	35.0
94-95	31.787405733171752	35.0	33.0	35.0	27.0	35.0
96-97	31.682014244517934	35.0	33.0	35.0	26.0	35.0
98-99	31.549550986314483	35.0	33.0	35.0	25.0	35.0
100	31.20831426259287	35.0	33.0	35.0	24.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.41111637756965536
1101	2	0.35286059699085115
1101	3	0.38029386395199083
1101	4	0.2822262181350368
1101	5	0.3218502886851198
1101	6	0.2843348225748912
1101	7	0.30337563200049544
1101	8	0.3138442974795126
1101	9	0.36581918892598253
1101	10-11	0.3812308386282126
1101	12-13	0.37229467778819014
1101	14-15	0.5123309130480109
1101	16-17	0.47426044509006715
1101	18-19	0.4945776819208092
1101	20-21	0.490611825112083
1101	22-23	0.4822528373012034
1101	24-25	0.5016601891736272
1101	26-27	0.5413506854061652
1101	28-29	0.5494163387066138
1101	30-31	0.5928665051439026
1101	32-33	0.7034743311719254
1101	34-35	0.6187752593204934
1101	36-37	0.6536464938749944
1101	38-39	0.6753131952359368
1101	40-41	0.6708982532491277
1101	42-43	0.7169285720597003
1101	44-45	0.7661831238852841
1101	46-47	0.8510166668529351
1101	48-49	0.8343122273749088
1101	50-51	0.8799453009622127
1101	52-53	0.8057541748294526
1101	54-55	0.9750342952811764
1101	56-57	0.9809071040065334
1101	58-59	0.9867141891632869
1101	60-61	1.014503682609309
1101	62-63	1.0245177968594348
1101	64-65	0.9387744199654406
1101	66-67	0.98825004260204
1101	68-69	1.0435813163889165
1101	70-71	1.012493621059761
1101	72-73	1.0013867180678844
1101	74-75	1.0080441318168525
1101	76-77	1.0815223129265377
1101	78-79	1.0449624037013692
1101	80-81	1.0392438416143577
1101	82-83	1.0202299290127357
1101	84-85	1.0488791687089432
1101	86-87	1.055689061709188
1101	88-89	1.0674137577540534
1101	90-91	1.0607975084155115
1101	92-93	1.1945612573006983
1101	94-95	1.135674752528427
1101	96-97	1.2148514936774646
1101	98-99	1.2547530455471012
1101	100	1.2598869887529105
1102	1	0.37501077849218944
1102	2	0.3576857098615136
1102	3	0.37687689330948615
1102	4	0.2647396255797787
1102	5	0.23733275453549396
1102	6	0.27458131726610446
1102	7	0.283498200429797
1102	8	0.2698145663448557
1102	9	0.3669173870913127
1102	10-11	0.3368328859395291
1102	12-13	0.3738403417255327
1102	14-15	0.46418812120980846
1102	16-17	0.500076833721522
1102	18-19	0.46944255386902967
1102	20-21	0.4725066087563974
1102	22-23	0.4990490029188521
1102	24-25	0.4691068962188112
1102	26-27	0.5243425345911348
1102	28-29	0.5592257508967933
1102	30-31	0.5509737470539591
1102	32-33	0.5571004356950127
1102	34-35	0.5534584530007649
1102	36-37	0.6351201386862684
1102	38-39	0.6259323392535165
1102	40-41	0.6674857077449516
1102	42-43	0.7259989466410772
1102	44-45	0.7480517913115108
1102	46-47	0.7452871663391036
1102	48-49	0.7841067415994232
1102	50-51	0.7661545944638561
1102	52-53	0.8217836852340028
1102	54-55	0.8885470346090543
1102	56-57	0.8979809207133513
1102	58-59	0.8787133547086299
1102	60-61	0.9189939336740096
1102	62-63	0.8888241175768599
1102	64-65	0.9486642880186196
1102	66-67	0.9748264777132434
1102	68-69	0.9097465112800407
1102	70-71	0.851607919089929
1102	72-73	0.8875959062090928
1102	74-75	0.9005030903052429
1102	76-77	0.9014457474976396
1102	78-79	0.9079317136550458
1102	80-81	0.9273201878935495
1102	82-83	0.9111578423242719
1102	84-85	0.9377618120653111
1102	86-87	0.9318944344856135
1102	88-89	0.9714962771011884
1102	90-91	0.9695782734982217
1102	92-93	0.996797914487999
1102	94-95	1.068300204211912
1102	96-97	1.045816096126856
1102	98-99	1.0723215690114607
1102	100	1.0735661975697823
1103	1	0.3554867732234186
1103	2	0.3278969470185089
1103	3	0.3416053122126712
1103	4	0.25062744209295573
1103	5	0.27795909574493294
1103	6	0.25345064641066983
1103	7	0.2775403988812215
1103	8	0.2750057088553959
1103	9	0.3130157097353745
1103	10-11	0.31487147799548865
1103	12-13	0.33537083365713016
1103	14-15	0.45041898121365165
1103	16-17	0.48553267135682177
1103	18-19	0.4305094870847128
1103	20-21	0.45322289923850434
1103	22-23	0.47775887775910064
1103	24-25	0.4586670107486768
1103	26-27	0.5538193072467905
1103	28-29	0.550324534564858
1103	30-31	0.5493163871945512
1103	32-33	0.5286447852856497
1103	34-35	0.4991029761886807
1103	36-37	0.5743251300394618
1103	38-39	0.5661014646536344
1103	40-41	0.5802846891574163
1103	42-43	0.6135704742395163
1103	44-45	0.5739029316204025
1103	46-47	0.5557318273566665
1103	48-49	0.49428922506286455
1103	50-51	0.5832063600510864
1103	52-53	0.5306439298179484
1103	54-55	0.5851817063324134
1103	56-57	0.5555461409156806
1103	58-59	0.5496933815325136
1103	60-61	0.6557094811299109
1103	62-63	0.6372129886901021
1103	64-65	0.6265236011259105
1103	66-67	0.613283116293573
1103	68-69	0.5920446345021446
1103	70-71	0.4948863299542694
1103	72-73	0.5454252019395227
1103	74-75	0.6201281045247384
1103	76-77	0.5828216880202817
1103	78-79	0.6952167328974426
1103	80-81	0.6338465612216098
1103	82-83	0.6598469239411955
1103	84-85	0.6700995049836536
1103	86-87	0.6408820637361146
1103	88-89	0.6560962019414163
1103	90-91	0.7069487689171865
1103	92-93	0.7313826861488764
1103	94-95	0.7888436541628856
1103	96-97	0.8170176471267041
1103	98-99	0.8139289546037354
1103	100	0.8421460587433955
1104	1	0.32251048175113084
1104	2	0.21146769637644525
1104	3	0.26327410944409735
1104	4	0.21183784542292727
1104	5	0.235838453411354
1104	6	0.20935954313092253
1104	7	0.2382885275397726
1104	8	0.21239213156275127
1104	9	0.3106036348078547
1104	10-11	0.30755539975608315
1104	12-13	0.31964714447813236
1104	14-15	0.44148439037855525
1104	16-17	0.4045417299779501
1104	18-19	0.33221790785940186
1104	20-21	0.3528968594374575
1104	22-23	0.4089788562274421
1104	24-25	0.3890517747576254
1104	26-27	0.4245549722393207
1104	28-29	0.46545725524368464
1104	30-31	0.47791835635455016
1104	32-33	0.47353508462862237
1104	34-35	0.532515192786363
1104	36-37	0.5154912242374436
1104	38-39	0.49896213784594323
1104	40-41	0.5360001206753324
1104	42-43	0.520317093587245
1104	44-45	0.5655097192193566
1104	46-47	0.594813915012459
1104	48-49	0.6082115534366395
1104	50-51	0.6332665946722997
1104	52-53	0.6216239951185187
1104	54-55	0.5960862840423502
1104	56-57	0.558368739565239
1104	58-59	0.6446262315335858
1104	60-61	0.6878079247978306
1104	62-63	0.650548284458246
1104	64-65	0.6296794608956162
1104	66-67	0.6690899664037957
1104	68-69	0.6487971135920176
1104	70-71	0.6390152707411048
1104	72-73	0.647000629168744
1104	74-75	0.6534648555816105
1104	76-77	0.5674410170108999
1104	78-79	0.6638991618845935
1104	80-81	0.6383392690674299
1104	82-83	0.639755284850601
1104	84-85	0.6439320574774072
1104	86-87	0.6486542537196414
1104	88-89	0.6872515192853186
1104	90-91	0.6630668192933271
1104	92-93	0.7309021762686214
1104	94-95	0.7406674451569621
1104	96-97	0.8048072344255601
1104	98-99	0.7816995128999018
1104	100	0.7705168574929502
1105	1	0.19030271592995973
1105	2	0.14499977975830092
1105	3	0.19298220356955653
1105	4	0.15634495537884163
1105	5	0.1479102888677204
1105	6	0.1715994456059704
1105	7	0.15063618815489832
1105	8	0.11989506152367824
1105	9	0.16879709399529474
1105	10-11	0.18435679919957693
1105	12-13	0.2185654995373696
1105	14-15	0.20137700390369417
1105	16-17	0.22725107042082726
1105	18-19	0.23314806205092253
1105	20-21	0.2239767947181548
1105	22-23	0.2803969077600641
1105	24-25	0.28143204280259226
1105	26-27	0.32343829165585447
1105	28-29	0.3082446453856349
1105	30-31	0.27759604826390216
1105	32-33	0.24966614865663672
1105	34-35	0.36027040910791186
1105	36-37	0.34454057890270207
1105	38-39	0.34845089992816014
1105	40-41	0.30025811581395345
1105	42-43	0.3238853771628598
1105	44-45	0.29057135370394604
1105	46-47	0.33578906547492693
1105	48-49	0.3529522178254112
1105	50-51	0.3436113761207551
1105	52-53	0.34251296880214
1105	54-55	0.3970417114697611
1105	56-57	0.4711210692868164
1105	58-59	0.44180985150341456
1105	60-61	0.5107655376168125
1105	62-63	0.4886504131089211
1105	64-65	0.48094057058889206
1105	66-67	0.48123400402622707
1105	68-69	0.48060318362861665
1105	70-71	0.4707082554359374
1105	72-73	0.40320701735078757
1105	74-75	0.401757053832803
1105	76-77	0.3230631474455734
1105	78-79	0.4063269287464166
1105	80-81	0.4374646168841494
1105	82-83	0.4269992384766894
1105	84-85	0.4002130264830086
1105	86-87	0.4396719474625357
1105	88-89	0.4777132018386112
1105	90-91	0.48964801947469994
1105	92-93	0.43179848414618505
1105	94-95	0.4704816373177998
1105	96-97	0.5304210147813251
1105	98-99	0.5250772560990491
1105	100	0.5113294168045286
1106	1	0.19600854666879997
1106	2	0.13910429536514357
1106	3	0.1652850853810932
1106	4	0.11168883409975905
1106	5	0.15707304908944764
1106	6	0.10853060616245358
1106	7	0.12259272335928983
1106	8	0.14999331126413296
1106	9	0.21280937056039306
1106	10-11	0.20364452214376882
1106	12-13	0.21798290062123726
1106	14-15	0.2714014401900613
1106	16-17	0.2453834652259559
1106	18-19	0.2358836799126962
1106	20-21	0.22258481614927206
1106	22-23	0.3030856380981106
1106	24-25	0.25183159224091156
1106	26-27	0.3065228781670939
1106	28-29	0.33544114051499463
1106	30-31	0.2825521711817558
1106	32-33	0.316701003024491
1106	34-35	0.2779159206519779
1106	36-37	0.30973136534966983
1106	38-39	0.3244385937354721
1106	40-41	0.397325687381489
1106	42-43	0.34229257718738637
1106	44-45	0.4104773120081049
1106	46-47	0.36616976805820656
1106	48-49	0.3865156742586393
1106	50-51	0.37253864138894244
1106	52-53	0.3798048083157468
1106	54-55	0.37297696443064154
1106	56-57	0.3613603130572187
1106	58-59	0.35868844344446416
1106	60-61	0.36135145574964866
1106	62-63	0.2856683121586272
1106	64-65	0.3565486793288386
1106	66-67	0.34695955933766953
1106	68-69	0.3572046871736063
1106	70-71	0.39316604791517307
1106	72-73	0.38421835722089526
1106	74-75	0.3972822590015994
1106	76-77	0.23366593849481632
1106	78-79	0.37293812699660833
1106	80-81	0.4237142640148406
1106	82-83	0.43759063215976823
1106	84-85	0.43301705354003417
1106	86-87	0.4377077118605541
1106	88-89	0.4268570615052809
1106	90-91	0.46711762086593467
1106	92-93	0.3985964843945027
1106	94-95	0.42069946033568684
1106	96-97	0.45475965969465904
1106	98-99	0.4740490442383951
1106	100	0.4952526494790277
1107	1	0.14770700450994667
1107	2	0.07767400921940038
1107	3	0.16344352683834273
1107	4	0.08278718333331625
1107	5	0.1591938918610154
1107	6	0.14361027425469075
1107	7	0.10128475193861419
1107	8	0.1030538429006711
1107	9	0.11835919714073384
1107	10-11	0.1730191698250394
1107	12-13	0.2164201757633748
1107	14-15	0.2222839099764542
1107	16-17	0.24141528634835652
1107	18-19	0.1861366603270156
1107	20-21	0.23301048209957997
1107	22-23	0.18730387945554838
1107	24-25	0.1713838999931525
1107	26-27	0.25237791380379093
1107	28-29	0.2447271953589123
1107	30-31	0.249719604170636
1107	32-33	0.3004256867625301
1107	34-35	0.2471279749812183
1107	36-37	0.24760700303496463
1107	38-39	0.23390836449076602
1107	40-41	0.2701046979711279
1107	42-43	0.29342573491459945
1107	44-45	0.25975316865774545
1107	46-47	0.29240462282536583
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2312	100	-0.2632565092025274
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2314	4	-0.19314299133094437
2314	5	-0.20435656352648124
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2314	7	-0.23685235322774645
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2314	80-81	-0.3055244354353164
2314	82-83	-0.29089223196360336
2314	84-85	-0.30212361329802206
2314	86-87	-0.3099475015718838
2314	88-89	-0.28970433724996525
2314	90-91	-0.3185500409633377
2314	92-93	-0.34278723541972056
2314	94-95	-0.300502447837939
2314	96-97	-0.3637427955556447
2314	98-99	-0.40165281883443527
2314	100	-0.5104949155400362
2315	1	-0.34755936079251626
2315	2	-0.42388634767819866
2315	3	-0.3922607326036882
2315	4	-0.2777470092209171
2315	5	-0.2615741009198089
2315	6	-0.24879457858987308
2315	7	-0.3063517516328176
2315	8	-0.32088238219733967
2315	9	-0.3262241359341189
2315	10-11	-0.3629742081856264
2315	12-13	-0.3820937021057489
2315	14-15	-0.5306956233536155
2315	16-17	-0.6144460034052344
2315	18-19	-0.5538492247282036
2315	20-21	-0.5380896337190606
2315	22-23	-0.47030327139962935
2315	24-25	-0.49037878489846065
2315	26-27	-0.46505191314648897
2315	28-29	-0.4643726429581747
2315	30-31	-0.5448969277671694
2315	32-33	-0.5103661045678791
2315	34-35	-0.5180798234327213
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2315	46-47	-0.665518263587785
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2315	64-65	-0.7274811343979835
2315	66-67	-0.6922331244203903
2315	68-69	-0.7165510371061572
2315	70-71	-0.6591382506248777
2315	72-73	-0.7126053556848362
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2315	80-81	-0.699895847357709
2315	82-83	-0.702316896797015
2315	84-85	-0.7289521800548755
2315	86-87	-0.7752880787804131
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2315	90-91	-0.8204965905244492
2315	92-93	-0.9010218735273909
2315	94-95	-0.9162195783256486
2315	96-97	-0.8353931244954254
2315	98-99	-0.8318120270216056
2315	100	-0.8524681446557878
2316	1	-0.44512558523476997
2316	2	-0.39310990300621995
2316	3	-0.3941832616672585
2316	4	-0.2613502809996575
2316	5	-0.339230383906461
2316	6	-0.302606186263489
2316	7	-0.34339374703782966
2316	8	-0.35262967265392575
2316	9	-0.45458831109137066
2316	10-11	-0.5104752517682698
2316	12-13	-0.5017463438675307
2316	14-15	-0.7039796820150386
2316	16-17	-0.7097703454903623
2316	18-19	-0.7179159383266551
2316	20-21	-0.7081037492262041
2316	22-23	-0.6219907883548501
2316	24-25	-0.5801033043686559
2316	26-27	-0.5703163036214107
2316	28-29	-0.6403242793663964
2316	30-31	-0.7155308598601238
2316	32-33	-0.6168530094914146
2316	34-35	-0.5878935839342745
2316	36-37	-0.6461622547165504
2316	38-39	-0.6799342592240905
2316	40-41	-0.6061101850323496
2316	42-43	-0.55054984087149
2316	44-45	-0.5823089618199475
2316	46-47	-0.6136752382122737
2316	48-49	-0.5206514718161728
2316	50-51	-0.5938113038079891
2316	52-53	-0.5163060399656345
2316	54-55	-0.6559733021860055
2316	56-57	-0.6700921564115987
2316	58-59	-0.6286130106726731
2316	60-61	-0.49691849808433375
2316	62-63	-0.44431886385529396
2316	64-65	-0.5328224234194039
2316	66-67	-0.43192158175541095
2316	68-69	-0.41662999033543713
2316	70-71	-0.48543756006834116
2316	72-73	-0.41373882988329314
2316	74-75	-0.43458619440058754
2316	76-77	-0.4863249435859771
2316	78-79	-0.5375745519208621
2316	80-81	-0.5016998828113515
2316	82-83	-0.5290455804388259
2316	84-85	-0.5588749961418245
2316	86-87	-0.6098502432131099
2316	88-89	-0.588093407570355
2316	90-91	-0.607749575144652
2316	92-93	-0.6137534651611887
2316	94-95	-0.6147476275686223
2316	96-97	-0.6299118979520557
2316	98-99	-0.6309977644144418
2316	100	-0.6684016503688319
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	32.0
3	50.0
4	81.0
5	150.0
6	327.0
7	782.0
8	1548.0
9	2647.0
10	4400.0
11	7704.0
12	12233.0
13	17299.0
14	19870.0
15	25431.0
16	34398.0
17	46280.0
18	47257.0
19	31381.0
20	22151.0
21	19793.0
22	20906.0
23	23746.0
24	27422.0
25	32549.0
26	41047.0
27	52416.0
28	67016.0
29	85836.0
30	108135.0
31	138227.0
32	177017.0
33	234755.0
34	339437.0
35	559537.0
36	1073933.0
37	2041095.0
38	1920191.0
39	402890.0
40	444.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	43.55421624459306	18.945441823629167	13.407194087544744	24.09314784423303
2	15.503954954238775	22.423386308342238	36.27896018302656	25.793698554392424
3	19.314561480612767	26.923402708254702	29.53890178652434	24.223134024608193
4	13.88257949347375	18.508238587687885	34.94685295152085	32.662328967317514
5	13.999648971855317	37.209572309769115	33.19495948713767	15.595819231237892
6	31.551946210237585	34.53099982945948	18.023973311390364	15.893080648912566
7	26.02643338782864	32.254800885763636	22.426771955913903	19.29199377049382
8	27.607067837824996	31.469411404854686	21.314750917260625	19.608769840059693
9	27.886097256784414	14.605506273024771	19.95443178267981	37.553964687511005
10-11	25.313500969254648	25.732182853609764	26.139340482777946	22.814975694357646
12-13	27.07977453802657	23.686837661607182	26.36777661488078	22.865611185485474
14-15	25.538909220174315	23.67349978476221	24.725377130765757	26.06221386429771
16-17	23.562419024062525	26.503958503672653	26.82697879047226	23.10664368179256
18-19	24.785139232656665	25.523077090204417	27.98649104439773	21.705292632741187
20-21	24.97566160003874	25.236060883302642	27.04828675726651	22.73999075939211
22-23	23.657708227189715	25.801300457689642	25.503172282993365	25.037819032127278
24-25	23.728699202203142	26.613690060283208	25.165903645688648	24.491707091825006
26-27	24.5199697894548	25.33835856430411	25.512525328424502	24.629146317816584
28-29	23.90452126042596	24.773539073177247	27.468779632263306	23.853160034133488
30-31	25.19693912816761	24.98157944352937	26.54061393216432	23.2808674961387
32-33	22.893536078874224	25.699866340606853	26.867514380295265	24.539083200223654
34-35	22.98584991414612	26.10732788617002	27.256725416923693	23.65009678276017
36-37	24.478149148036273	25.873180688141566	26.100382795335253	23.54828736848691
38-39	24.850261486529863	24.864076807688306	25.97784954415003	24.307812161631798
40-41	23.844350212218835	25.88412080109739	26.17611679682768	24.0954121898561
42-43	25.579673582057026	25.098194354732627	25.89918071570069	23.422951347509656
44-45	25.30723372798781	24.8813396006346	26.05854944831724	23.75287722306035
46-47	24.047616492230883	25.212977268289055	27.514328061430433	23.225078178049625
48-49	24.74071916928569	25.873884427051554	26.726030826215208	22.659365577447552
50-51	23.41092141267776	26.371733932159202	25.994784618295302	24.222560036867737
52-53	23.178144083793672	26.478563192216242	24.75610800836162	25.587184715628464
54-55	24.039930467719714	26.133726788457025	24.559753575231962	25.266589168591302
56-57	24.540767527053063	24.838581000001835	24.882084271453085	25.738567201492017
58-59	24.08204424600051	24.787788672047213	26.431475256167342	24.698691825784934
60-61	24.527801069979375	25.299971918441727	25.70185240097323	24.470374610605663
62-63	22.581341639992804	25.757171575119497	26.26340117704436	25.398085607843335
64-65	22.592009300636775	25.798579747109486	26.153394275610946	25.45601667664279
66-67	24.180935161098454	25.685102708226676	25.16617082692797	24.967791303746903
68-69	24.646998551038855	24.80562449008066	25.086254776725763	25.46112218215472
70-71	23.42196313148731	25.322264097602282	25.49620143984702	25.75957133106338
72-73	24.678666469118944	24.8140464282517	25.377914723256062	25.129372379373294
74-75	24.896780428962003	24.42430154669671	25.507230081999744	25.171687942341542
76-77	23.497447850880178	25.229828231794578	26.546598047426123	24.72612586989913
78-79	23.82554317014817	25.207313291079803	26.22345027036539	24.743693268406634
80-81	22.810128195949616	25.261553269437137	26.115728298980695	25.81259023563255
82-83	22.787410513114317	25.773872227847733	24.798085362012504	26.640631897025447
84-85	23.232226854826713	25.4767528524809	24.459680536798384	26.831339755894007
86-87	23.40405328484703	24.352757215585473	25.14079486454257	27.102394635024922
88-89	23.330474409468398	23.953569732699997	26.10596900373331	26.60998685409829
90-91	23.89544714843812	24.379730262904307	25.531592696710646	26.193229891946924
92-93	22.440910048148364	24.82054797801617	25.881717613986705	26.856824359848762
94-95	21.841038843956465	25.124770943237113	26.3677456141941	26.666444598612323
96-97	23.21123438342867	24.61522473655344	25.493594806503296	26.679946073514593
98-99	23.288629816215433	24.14437544148464	25.43573887956057	27.131255862739355
100	22.895700456352305	24.777546738907503	25.72986985314453	26.596882951595664
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1233.0
1	776.5
2	376.0
3	470.5
4	423.5
5	499.5
6	838.5
7	1643.0
8	2592.0
9	2749.0
10	2193.5
11	1883.5
12	2125.5
13	2438.0
14	2753.0
15	3141.0
16	3563.0
17	4019.5
18	4619.0
19	5321.5
20	6091.0
21	7079.0
22	8219.0
23	9799.5
24	11880.0
25	14576.0
26	19098.5
27	24548.0
28	29568.0
29	37661.0
30	46067.0
31	53367.5
32	63114.0
33	73683.5
34	86547.0
35	97859.0
36	108720.0
37	120275.5
38	126704.0
39	132100.0
40	139175.0
41	144671.5
42	149363.5
43	164429.5
44	186010.0
45	203815.0
46	229626.5
47	263180.0
48	341940.5
49	453186.0
50	608349.0
51	586384.5
52	409277.0
53	339765.0
54	300219.5
55	271061.0
56	250690.5
57	228752.5
58	217611.0
59	200706.5
60	169306.0
61	136837.5
62	109465.5
63	85573.0
64	63434.0
65	51996.0
66	43676.5
67	33485.5
68	25117.0
69	19984.5
70	16358.0
71	13617.0
72	12440.5
73	12916.5
74	9740.0
75	7071.5
76	5627.0
77	4112.5
78	3041.5
79	2003.5
80	1540.5
81	1292.5
82	1094.0
83	914.0
84	652.0
85	366.5
86	231.0
87	125.5
88	64.0
89	35.0
90	26.0
91	17.0
92	14.0
93	11.5
94	3.0
95	2.0
96	2.5
97	1.5
98	1.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.06282382902599637
3	0.02698806988575094
4	0.007486506292264567
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-11	7.852978628249546E-5
12-13	1.505154237081163E-4
14-15	3.402957405574803E-4
16-17	3.402957405574803E-4
18-19	0.0
20-21	0.0029186903901660816
22-23	0.005372746211494064
24-25	7.7875371396808E-4
26-27	4.515462711243489E-4
28-29	0.005346569616066566
30-31	0.0022446430579079954
32-33	0.0012695648782336767
34-35	0.00138735955765742
36-37	0.003913401016411024
38-39	0.0045089185623866145
40-41	8.965483933918232E-4
42-43	0.0027223659244598425
44-45	2.1595691227686254E-4
46-47	0.0021072159319136283
48-49	6.5441488568746224E-6
50-51	0.001681846256216778
52-53	0.006603046196586494
54-55	0.014181170572847304
56-57	0.024959383740119807
58-59	0.027426527859161543
60-61	0.02549600394638353
62-63	0.007839890330535797
64-65	0.006622678643157118
66-67	9.816223285311934E-5
68-69	0.0014593451950830408
70-71	2.355893588474864E-4
72-73	8.507393513937009E-4
74-75	0.005091347810648456
76-77	0.0011452260499530589
78-79	0.0013677271110867958
80-81	0.0
82-83	0.0015378749813655362
84-85	3.2720744284373105E-5
86-87	5.497085039774683E-4
88-89	1.177946794237432E-4
90-91	6.216941414030891E-4
92-93	9.161808399624471E-4
94-95	0.00147243349279679
96-97	1.505154237081163E-4
98-99	0.0
100	5.758850994049667E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	7640413.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.02610398322207
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.76520598983278	28.015773972663993
2	12.155131399940037	8.758040554879349
3	3.738140309635544	4.040118944964122
4	1.7064481518589976	2.459067142433975
5	1.0047220883130863	1.8098111213903634
6	0.6441525485460592	1.3923784016986696
7	0.4474483995639114	1.128387579887104
8	0.32194809460682694	0.9278828426804611
9	0.24941416459603477	0.8086878565762692
>10	1.6040135365844315	11.062670230236058
>50	0.17618098491962936	4.440438367157157
>100	0.15452730576637713	11.386228577115372
>500	0.01974624456625984	4.929808365064875
>1k	0.011022813400388935	7.1080673989256615
>5k	9.489839350923311E-4	2.4766322157737832
>10k+	9.489839350923311E-4	9.256006428552716
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	99678	1.304615339511097	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	90043	1.1785095910391232	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	44165	0.5780446685277354	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	39741	0.5201420394421087	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	35098	0.4593730731571709	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	33362	0.4366517883261023	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	32733	0.42841924906415396	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	31169	0.40794915143985017	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	29044	0.38013651879813304	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	27927	0.36551689025187517	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	26108	0.3417092767105652	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	24565	0.3215140333382502	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	20101	0.26308787234407355	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18165	0.23774892797025501	No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC	15322	0.2005388975700659	No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC	14965	0.19586637528625742	No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT	13993	0.18314454990849316	No Hit
ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA	13123	0.17175773089753132	No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG	12863	0.16835477349195652	No Hit
GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC	12779	0.16725535648400158	No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA	12616	0.16512196395666046	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12374	0.16195459590993314	No Hit
GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT	11501	0.15052851200583003	No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA	11387	0.14903644606646263	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10639	0.1392463993765782	No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG	10455	0.13683815259724832	No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC	9891	0.12945635268669378	No Hit
TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC	9634	0.1260926601742602	No Hit
GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA	9429	0.12340955914294162	No Hit
GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG	9159	0.11987571876022933	No Hit
CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC	9087	0.11893336132483938	No Hit
CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA	8963	0.11731041240833447	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC	8878	0.1161979071026658	No Hit
GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG	8807	0.1152686379649896	No Hit
ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG	8586	0.112376124170251	No Hit
ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC	8528	0.11161700290285355	No Hit
ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA	7941	0.10393417214488274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0020548627410586313	0.0	0.0	5.235319085499697E-5	0.0
2	0.0033506042147198063	2.6176595427498486E-5	0.0	6.544148856874622E-5	0.0
3	0.003494575489571048	2.6176595427498486E-5	0.0	6.544148856874622E-5	0.0
4	0.0039657542072660205	5.235319085499697E-5	0.0	1.0470638170999395E-4	0.0
5	0.016334195546759057	5.235319085499697E-5	0.0	2.2250106113373715E-4	0.0
6	0.033807072994614294	5.235319085499697E-5	0.0	3.926489314124773E-4	0.0
7	0.03758959103388783	5.235319085499697E-5	0.0	4.5809041998122355E-4	0.0
8	0.04359711968449873	6.544148856874622E-5	0.0	4.711787176949728E-4	0.0
9	0.047431990914627256	9.161808399624471E-5	0.0	5.104436108362205E-4	0.0
10-11	0.08448496174225137	9.161808399624471E-5	0.0	9.227249888193217E-4	0.0
12-13	0.1462813593977184	9.161808399624471E-5	0.0	0.001904347317350515	0.0
14-15	0.21935986968243731	1.3088297713749245E-4	0.0	0.0030233967718760755	1.9632446570623865E-5
16-17	0.25172722992853924	1.3088297713749245E-4	0.0	0.003632002615565415	2.6176595427498486E-5
18-19	0.2672106861239046	1.4397127485124168E-4	0.0	0.0040377398446916415	2.6176595427498486E-5
20-21	0.31923012538720097	1.5705957256499092E-4	0.0	0.005104436108362205	2.6176595427498486E-5
22-23	0.3338039448914607	2.0286861456311327E-4	0.0	0.006236573860601514	2.6176595427498486E-5
24-25	0.40086838237671185	2.2904520999061177E-4	0.0	0.01177946794237432	2.6176595427498486E-5
26-27	0.5342970334195285	2.4867765656123565E-4	0.0	0.029363595920796428	2.6176595427498486E-5
28-29	0.5654864468713929	2.4867765656123565E-4	0.0	0.05077605098049019	2.6176595427498486E-5
30-31	0.576081423870673	3.402957405574803E-4	0.0	0.10218688440009722	2.6176595427498486E-5
32-33	0.5859761769422673	3.402957405574803E-4	0.0	0.1926270216021045	2.6176595427498486E-5
34-35	0.5940974656736489	3.7956063369872807E-4	0.0	0.3167695254170161	2.6176595427498486E-5
36-37	0.6071072336011155	4.188255268399758E-4	0.0	0.49637369079394006	2.6176595427498486E-5
38-39	0.6202544286545766	4.450021222674743E-4	0.0	0.7616080439630686	5.235319085499697E-5
40-41	0.6322629418069415	4.84267015408722E-4	0.0	1.1170927540173547	5.235319085499697E-5
42-43	0.6563650420468108	5.038994619793459E-4	0.0	1.3913842092043978	5.235319085499697E-5
44-45	0.6839944385205354	5.300760574068445E-4	0.0	1.691485787482954	5.235319085499697E-5
46-47	0.6948511814740905	5.36620206263719E-4	0.0	2.0329856514300992	5.235319085499697E-5
48-49	0.7024816590412062	5.36620206263719E-4	0.0	2.347700314106057	5.235319085499697E-5
50-51	0.7149286301669818	6.347824391168383E-4	0.0	2.6815631563372295	5.235319085499697E-5
52-53	0.7188682077788203	6.413265879737129E-4	0.0	3.067366908045416	5.88973397118716E-5
54-55	0.7322509921911289	6.609590345443368E-4	0.0	3.427759729742358	6.544148856874622E-5
56-57	0.7567915504044087	6.805914811149606E-4	0.0	3.8172282048103945	7.198563742562084E-5
58-59	0.7650895311549257	6.871356299718353E-4	0.0	4.220674981836715	7.852978628249546E-5
60-61	0.7716140475652298	7.067680765424592E-4	0.0	4.591898369891785	7.852978628249546E-5
62-63	0.7769867937767239	7.133122253993338E-4	0.0	4.966904275986128	9.161808399624471E-5
64-65	0.7811881373428373	7.722095651112054E-4	0.0	5.375062840189399	9.816223285311933E-5
66-67	0.7868750026994613	7.918420116818292E-4	0.0	5.8101374886409936	1.1125053056686856E-4
68-69	0.7936089318731854	8.180186071093277E-4	0.0	6.305287423598698	1.177946794237432E-4
70-71	0.8023257381505424	8.31106904823077E-4	0.0	6.889850064387881	1.177946794237432E-4
72-73	0.822750026732848	8.441952025368261E-4	0.0	7.387048317937786	1.4397127485124168E-4
74-75	0.8423432084103307	8.6382764910745E-4	0.0	7.870418784953117	1.4397127485124168E-4
76-77	0.8484423551449378	9.161808399624471E-4	0.0	8.392196861609445	1.4397127485124168E-4
78-79	0.8530690683867482	9.292691376761963E-4	0.0	8.909354769172818	1.4397127485124168E-4
80-81	0.8618840368969584	9.292691376761963E-4	0.0	9.482458605313614	1.4397127485124168E-4
82-83	0.8653589799399588	9.292691376761963E-4	0.0	10.128784923013978	1.4397127485124168E-4
84-85	0.8775834500046006	9.358132865330709E-4	0.0	10.736788705008486	1.4397127485124168E-4
86-87	0.8980666359266181	9.554457331036948E-4	0.0	11.346866982190623	1.4397127485124168E-4
88	0.9032234252258353	0.0010077989239586917	0.0	11.829177297091139	1.4397127485124168E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATC	1740	0.0	25.39516	3
GATCTAC	2500	0.0	19.7571	1
AGTACTT	20500	0.0	18.682459	12-13
CCGATCT	2490	0.0	18.49608	4
CGTTAAC	655	0.0	17.95447	1
GAGTACT	19480	0.0	17.779058	12-13
CGATCTC	1930	0.0	17.774202	5
TCTACAC	3480	0.0	17.560484	3
CATGGGG	16810	0.0	16.634212	4
TACACTC	3145	0.0	16.585436	5
GTACTTT	22035	0.0	15.825211	14-15
GTATAAT	2775	0.0	15.764995	1
TACTTTT	25165	0.0	15.350889	14-15
GTATATA	2090	0.0	15.080036	1
GTGATCG	1325	0.0	14.895795	8
TGATCGC	1330	0.0	14.839794	9
TATCACG	385	9.107134E-8	14.662039	2
ACTTTTT	24570	0.0	14.461416	16-17
TCTCGCG	1060	0.0	13.743143	8
GTATAAA	3905	0.0	13.612293	1
>>END_MODULE