FastQCFastQC Report
Fri 27 May 2016
ERR765478_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765478_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11038360
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA494520.4480013335314304No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT483800.43828974594052017No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA311760.2824332600132628No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC290210.26291043234683414No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG243590.22067589750651365No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG198030.17940165024514512No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG193480.1752796611090778No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC159120.14415184864418265No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC156190.14149746882689096No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT155680.14103544367098012No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA137960.124982334332274No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT137010.12412169923793027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC129500.11731815233422356No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG32000.021.87911
TCTACAC109600.020.4064733
ATCTACA103150.019.2228052
TACACTC96450.019.095795
GTACATA28000.017.7885681
GTACTAG27600.017.1951311
CATGGGG192500.016.8903664
CTACACT129950.016.8851244
TAGTACT34700.015.8423684
GTATAAA59450.015.491641
GTCCTAC33200.015.4270051
GTACAAG51550.015.2223251
GTACAAA53950.015.1548271
TATTCGC6950.014.8716199
TGATCGC23150.014.8146799
TATAAGG42700.014.7451922
GTATTAG47200.014.7337371
GTGATCG23950.014.3200868
GTATAAT24750.014.2389931
ATAAGGT32650.013.8152773
Produced by FastQC (version 0.11.5)