##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765478_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 11038360 Sequences flagged as poor quality 0 Sequence length 100 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.07390690283702 33.0 33.0 33.0 33.0 33.0 2 32.061144952692246 33.0 33.0 33.0 33.0 33.0 3 32.02892069111716 33.0 33.0 33.0 33.0 33.0 4 35.83601775988462 37.0 37.0 37.0 33.0 37.0 5 35.84747833917357 37.0 37.0 37.0 33.0 37.0 6 35.80080627919365 37.0 37.0 37.0 33.0 37.0 7 35.76138167263978 37.0 37.0 37.0 33.0 37.0 8 35.74900429049243 37.0 37.0 37.0 33.0 37.0 9 35.7850157088553 37.0 37.0 37.0 33.0 37.0 10-11 35.741161957029846 37.0 37.0 37.0 33.0 37.0 12-13 35.73247950782544 37.0 37.0 37.0 33.0 37.0 14-15 37.58036877760827 40.0 37.0 40.0 33.0 40.0 16-17 37.61110780949344 40.0 37.0 40.0 33.0 40.0 18-19 37.623870303197215 40.0 37.0 40.0 33.0 40.0 20-21 37.63743422936015 40.0 37.0 40.0 33.0 40.0 22-23 37.605324341659454 40.0 37.0 40.0 33.0 40.0 24-25 37.586960789465095 40.0 37.0 40.0 33.0 40.0 26-27 37.59210725143953 40.0 37.0 40.0 33.0 40.0 28-29 37.548365472769504 40.0 37.0 40.0 33.0 40.0 30-31 37.48595516000565 40.0 37.0 40.0 33.0 40.0 32-33 37.364940126975384 40.0 37.0 40.0 33.0 40.0 34-35 37.30871768994669 40.0 37.0 40.0 33.0 40.0 36-37 37.22295839236988 40.0 37.0 40.0 33.0 40.0 38-39 37.13511758087252 40.0 37.0 40.0 33.0 40.0 40-41 37.04929292032512 40.0 37.0 40.0 33.0 40.0 42-43 36.921396792639484 40.0 37.0 40.0 33.0 40.0 44-45 36.80310539790331 40.0 37.0 40.0 33.0 40.0 46-47 36.64549317108701 37.0 37.0 40.0 33.0 40.0 48-49 36.45871347736439 37.0 37.0 40.0 33.0 40.0 50-51 36.289466234114485 37.0 37.0 40.0 33.0 40.0 52-53 36.1339054895836 37.0 37.0 40.0 33.0 40.0 54-55 35.97431420971956 37.0 37.0 40.0 33.0 40.0 56-57 35.821062639739964 37.0 37.0 40.0 27.0 40.0 58-59 35.650231601433546 37.0 37.0 40.0 27.0 40.0 60-61 35.45231551607304 37.0 33.0 40.0 27.0 40.0 62-63 35.235260310408435 37.0 33.0 40.0 27.0 40.0 64-65 35.01382786935741 37.0 33.0 37.0 27.0 40.0 66-67 34.7954900909193 37.0 33.0 37.0 27.0 40.0 68-69 34.60288398820114 37.0 33.0 37.0 27.0 40.0 70-71 34.377888880232206 37.0 33.0 37.0 27.0 40.0 72-73 34.15316450994531 37.0 33.0 37.0 27.0 37.0 74-75 33.93769101569436 37.0 33.0 37.0 27.0 37.0 76-77 33.78872726564453 37.0 33.0 37.0 27.0 37.0 78-79 33.64120680064792 37.0 33.0 37.0 27.0 37.0 80-81 33.4663445475596 37.0 33.0 37.0 27.0 37.0 82-83 33.359022581253015 37.0 33.0 37.0 27.0 37.0 84-85 33.2365933435764 37.0 33.0 37.0 27.0 37.0 86-87 33.14050868969666 37.0 33.0 37.0 27.0 37.0 88-89 33.07048791668328 37.0 33.0 37.0 27.0 37.0 90-91 32.97346118445131 37.0 33.0 37.0 27.0 37.0 92-93 32.88292622273599 37.0 33.0 37.0 27.0 37.0 94-95 32.79693219826134 37.0 33.0 37.0 27.0 37.0 96-97 32.7335619602912 37.0 33.0 37.0 27.0 37.0 98-99 32.645685636272056 35.0 33.0 37.0 24.5 37.0 100 32.577057280248155 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 6.0 5 17.0 6 44.0 7 391.0 8 1501.0 9 2412.0 10 3130.0 11 4972.0 12 8844.0 13 16673.0 14 24568.0 15 25959.0 16 27525.0 17 30396.0 18 34904.0 19 40287.0 20 47922.0 21 58936.0 22 67969.0 23 64969.0 24 63859.0 25 67542.0 26 76503.0 27 89261.0 28 107652.0 29 135771.0 30 175798.0 31 232309.0 32 317466.0 33 441055.0 34 617005.0 35 916612.0 36 1726543.0 37 3617772.0 38 1989548.0 39 2239.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.50251831595181 18.274433690387358 13.053018060201275 25.170029933459553 2 15.980422480571695 20.782192984411154 36.95298014217012 26.284404392847026 3 18.640248695860627 25.19046699690942 28.989134459512044 27.180149847717907 4 13.578159139763905 17.05206478062173 35.415286750348145 33.95448932926622 5 14.625328476413127 36.53769550009967 33.21610576487432 15.620870258612879 6 32.72676143005023 35.533097937846385 16.99062084215825 14.749519789945131 7 27.795588199455906 31.56980111465951 21.61112046753406 19.023490218350524 8 27.3112551895554 32.55559721866012 20.217793048679606 19.915354543104872 9 27.12704604669534 14.917668929080044 19.196257415050784 38.75902760917383 10-11 25.743081399773153 25.249837838229595 26.46038451364152 22.546696248355737 12-13 26.71082026677876 22.93439876938241 26.384548972854667 23.970231990984168 14-15 24.743979178066308 23.507119717059417 24.130115343221277 27.618785761652997 16-17 24.26995153258199 26.22206084233236 25.290848446617396 24.21713917846825 18-19 24.89921872285628 25.595914472228998 26.533752356712064 22.971114448202663 20-21 25.48944908702564 25.00321737014769 25.669984208273043 23.837349334553632 22-23 24.576673437548134 25.0462198730505 25.10766969936924 25.26943699003213 24-25 24.542405943951323 25.59490137923427 24.862100356502083 25.000592320312325 26-27 24.92389268592357 25.076782353650064 25.056327295429032 24.94299766499734 28-29 24.39560350932312 24.736845849290532 26.194492048772105 24.673058592614243 30-31 25.170934472894974 24.704355182527713 25.678855085052803 24.445855259524507 32-33 24.047279056060884 25.134278326039155 25.57442089413525 25.24402172376471 34-35 24.456010427506943 25.30045454977916 25.87983902060447 24.36369600210942 36-37 24.953186725667848 25.21995068957973 25.22502517647774 24.601837408274683 38-39 25.006307786356853 24.596556521775376 25.352303856387348 25.04483183548043 40-41 24.680920050743595 25.038425161487172 25.522961441083336 24.757693346685894 42-43 25.601495039188148 24.671580664159833 25.323997644180672 24.402926652471347 44-45 25.12390261406312 24.72518062665568 25.445018682235272 24.70589807704593 46-47 24.662840818857806 24.691325358870316 26.077512563417404 24.56832125885447 48-49 25.081950270310045 25.258199931790987 25.493161722464713 24.166688075434255 50-51 24.20336283634106 25.641528556371533 25.00350115165349 25.15160745563392 52-53 24.235775223974557 25.430404356472806 24.615146987415308 25.718673432137333 54-55 24.68714285856694 25.025322463229298 24.690622799832056 25.596911878371703 56-57 24.774425283125122 24.473980669134203 24.776808397806175 25.9747856499345 58-59 24.54770534741524 24.493835530510548 25.51139514262793 25.447063979446288 60-61 24.52456363097305 24.796168419343402 25.136534085678306 25.542733864005235 62-63 23.738544241760586 24.983464654864456 25.25832356812791 26.01966753524705 64-65 23.891368208581866 24.762430138737347 25.25208363357987 26.094118019100915 66-67 24.584051670244307 24.794768864339698 24.88977145017137 25.73140801524463 68-69 24.62750257621137 24.340626694786494 24.916330887918182 26.11553984108395 70-71 23.903976949031218 24.481225216980608 25.242704547333656 26.37209328665452 72-73 24.6032276344312 24.2712055163546 25.240204357683844 25.885362491530362 74-75 24.639995585986156 24.04168470731745 25.45040019214369 25.8679195145527 76-77 24.06979659293644 24.534382990359177 25.70485041898124 25.69096999772314 78-79 24.16222265278534 24.448449476727642 25.70296312238977 25.686364748097247 80-81 23.742423155939832 24.403785365494805 25.704915855474965 26.1488756230904 82-83 23.813568765656353 24.665913198675465 25.138324039554803 26.382193996113372 84-85 23.888468403966723 24.510853531444468 25.020480049182016 26.580198015406797 86-87 23.875086910539977 23.980686415809558 25.40898404823259 26.73524262541787 88-89 23.877414731914936 23.669718944367997 25.799873612320855 26.652992711396212 90-91 24.126674148598116 23.937448564741114 25.448123181533717 26.487754105127053 92-93 23.743487245914146 24.160726585589668 25.48249258095719 26.613293587539 94-95 23.255128156287203 24.23248713898225 25.876938019830657 26.63544668489989 96-97 23.822991683200822 24.085342790861713 25.397909145290186 26.693756380647276 98-99 23.62894920320169 23.944037493151043 25.412980369220424 27.014032934426847 100 23.54998709046189 24.180969075994145 25.47897104186767 26.790072791676295 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 204.0 1 207.5 2 313.0 3 405.5 4 349.5 5 319.5 6 416.0 7 772.0 8 1278.0 9 1309.0 10 936.0 11 795.0 12 873.5 13 1040.0 14 1263.5 15 1445.5 16 1664.5 17 2039.5 18 2516.5 19 3065.0 20 3840.5 21 4924.5 22 6373.0 23 8470.0 24 10884.0 25 14300.0 26 19799.5 27 26240.5 28 33119.5 29 42551.5 30 53227.5 31 64295.0 32 76943.0 33 91102.0 34 107158.0 35 121896.5 36 136900.0 37 154458.5 38 169655.5 39 185921.5 40 201826.5 41 216300.0 42 231912.0 43 259803.5 44 294824.5 45 325797.5 46 367280.0 47 413283.5 48 503013.5 49 585478.0 50 632143.5 51 611904.5 52 536501.0 53 510202.0 54 485210.0 55 449803.0 56 422298.0 57 388226.5 58 364605.0 59 336209.5 60 287872.0 61 240032.5 62 199581.0 63 158385.5 64 121839.5 65 101019.5 66 80941.5 67 61378.0 68 49435.5 69 41377.0 70 36146.5 71 32123.0 72 28062.5 73 27807.5 74 22235.5 75 16385.5 76 12801.5 77 9051.0 78 6676.5 79 4418.5 80 3023.5 81 2325.5 82 1760.5 83 1295.0 84 928.0 85 609.5 86 403.5 87 239.0 88 124.0 89 73.5 90 50.5 91 38.5 92 27.0 93 20.5 94 18.5 95 13.5 96 8.0 97 5.5 98 5.0 99 4.0 100 12.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02006638667338264 2 0.005417471435974185 3 0.005625835721973192 4 0.005009802180758736 5 0.023191850963367747 6 0.0043665906891965835 7 0.0017303295054700155 8 7.519232929529386E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0020202276425121124 18-19 0.004271467862979645 20-21 0.005603187430016778 22-23 0.008153385104308974 24-25 0.011392090854076149 26-27 0.017846854061654087 28-29 0.02935671603390359 30-31 0.01980819614507952 32-33 0.027830221156041297 34-35 0.019613420834254363 36-37 0.025017303295054697 38-39 0.02171065266941828 40-41 0.029651143829336965 42-43 0.016850329215571877 44-45 0.022421809036849677 46-47 0.023092198478759526 48-49 0.028641030008080912 50-51 0.024509981555231028 52-53 0.027273073173913517 54-55 0.03343793824444936 56-57 0.021583822234462365 58-59 0.02260752503089227 60-61 0.020727716798509925 62-63 0.019500179374472296 64-65 0.020750365090466336 66-67 0.01170463728307466 68-69 0.007555470196659649 70-71 0.01764301943404636 72-73 0.010359328740863679 74-75 0.007129682307879069 76-77 0.011152018959338162 78-79 0.01027779488982059 80-81 0.0048512641370638395 82-83 0.004620251559108418 84-85 0.0016170880456879465 86-87 0.0038909765581118935 88-89 9.104613366478354E-4 90-91 0.0019205751579038917 92-93 0.0019115158411213262 94-95 0.00408122221054577 96-97 8.968723614739871E-4 98-99 0.0016669142879920567 100 9.059316782565526E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.103836E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.295949540603935 #Duplication Level Percentage of deduplicated Percentage of total 1 76.42500130619759 33.08893000193721 2 12.847814526798599 11.125166589186207 3 4.258702772978175 5.531537411018793 4 1.9435733796786563 3.365954199001126 5 1.101414546495288 2.384339431417358 6 0.6675641320812824 1.7341693786624959 7 0.47426143095381756 1.437351928854177 8 0.3398030978638421 1.1769678223083047 9 0.2497067400208898 0.9730161374303834 >10 1.4596244660953352 11.710788477724158 >50 0.1218314014871715 3.6502433621709214 >100 0.08834205960437869 7.768390160416411 >500 0.012432914865940876 3.762212901092192 >1k 0.009045738179785373 7.735454019392372 >5k 5.876577994406216E-4 1.647102056849666 >10k+ 2.938288997203108E-4 2.9083761225382676 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 49452 0.4480013335314304 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 48380 0.43828974594052017 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 31176 0.2824332600132628 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 29021 0.26291043234683414 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 24359 0.22067589750651365 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 19803 0.17940165024514512 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 19348 0.1752796611090778 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 15912 0.14415184864418265 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 15619 0.14149746882689096 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 15568 0.14103544367098012 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13796 0.124982334332274 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 13701 0.12412169923793027 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 12950 0.11731815233422356 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.073217398236695E-4 0.0 0.0 0.0 9.059316782565526E-6 2 8.334571439960284E-4 0.0 0.0 0.0 9.059316782565526E-6 3 8.515757775611595E-4 0.0 0.0 0.0 9.059316782565526E-6 4 0.0010055841628647734 0.0 0.0 0.0 9.059316782565526E-6 5 0.0034878369612877276 0.0 0.0 3.62372671302621E-5 9.059316782565526E-6 6 0.007039089140053413 0.0 0.0 1.1777111817335184E-4 9.059316782565526E-6 7 0.007972198768657663 0.0 0.0 1.1777111817335184E-4 9.059316782565526E-6 8 0.009675350323779981 0.0 0.0 1.358897517384829E-4 9.059316782565526E-6 9 0.010816824238383238 0.0 0.0 1.6306770208617948E-4 9.059316782565526E-6 10-11 0.02178312720367881 0.0 0.0 3.533133545200555E-4 9.059316782565526E-6 12-13 0.040218836856199654 0.0 0.0 7.519232929529386E-4 1.7665667726002776E-4 14-15 0.06271312042730985 9.059316782565526E-6 0.0 0.0011913001569073666 2.446015531292692E-4 16-17 0.07321739823669458 1.811863356513105E-5 0.0 0.0013679568341673945 2.8536847865081406E-4 18-19 0.07938226330723042 1.811863356513105E-5 0.0 0.0015129059026884428 3.125464289985107E-4 20-21 0.10267829641359767 1.811863356513105E-5 0.0 0.0021108208103377676 3.4878369612877276E-4 22-23 0.10961773306904286 1.811863356513105E-5 0.0 0.0028718034200732718 3.759616464764693E-4 24-25 0.14095390981993702 1.811863356513105E-5 0.0 0.0070617374320098274 3.895506216503176E-4 26-27 0.22788258400704453 1.811863356513105E-5 0.0 0.019676836051732323 4.39376863954428E-4 28-29 0.24482803604883335 1.811863356513105E-5 0.0 0.0353449244271794 4.665548143021246E-4 30-31 0.24913574117894324 1.811863356513105E-5 0.0 0.08365826082860135 4.756141310846901E-4 32-33 0.25302218807866383 1.811863356513105E-5 0.0 0.1672123395142032 5.299700317800833E-4 34-35 0.25582151696447664 2.2648291956413815E-5 0.0 0.27441576466069234 5.843259324754764E-4 36-37 0.26069089973510556 3.170760873897934E-5 0.0 0.4483682358611243 5.933852492580419E-4 38-39 0.2657822357669074 3.62372671302621E-5 0.0 0.7187163672864447 5.979149076493247E-4 40-41 0.2704387245931461 3.62372671302621E-5 0.0 1.0771255874966934 6.069742244318902E-4 42-43 0.2803949137371856 3.62372671302621E-5 0.0 1.3546441681554144 6.341521747795869E-4 44-45 0.2915922292804366 3.62372671302621E-5 0.0 1.643622784544081 6.70389441909849E-4 46-47 0.29574592602524286 3.62372671302621E-5 0.0 1.9852224424642793 7.156860258226766E-4 48-49 0.2984773100351864 3.62372671302621E-5 0.0 2.314750560771709 7.609826097355042E-4 50-51 0.3041348533658985 5.8885559086675925E-5 0.0 2.6891811827119243 7.609826097355042E-4 52-53 0.3059240684304553 6.341521747795868E-5 0.0 3.0823192938081383 8.379868023873112E-4 54-55 0.3115317855188633 7.700419265180698E-5 0.0 3.4433828938356785 8.78753727908856E-4 56-57 0.32321377450998157 8.153385104308974E-5 0.0 3.8409555404969575 9.014020198652698E-4 58-59 0.32684656053979033 9.059316782565526E-5 0.0 4.265235053033241 9.195206534304009E-4 60-61 0.3292971057294743 9.965248460822078E-5 0.0 4.67785069521197 9.331096286042492E-4 62-63 0.33109085045242226 9.965248460822078E-5 0.0 5.106750459307361 9.693468957345112E-4 64-65 0.332463336944981 1.0871180139078632E-4 0.0 5.5595351120999865 9.829358709083596E-4 66-67 0.3341347808913643 1.2683043495591737E-4 0.0 6.026588188825151 0.0010101138212560562 68-69 0.3360598857076595 1.2683043495591737E-4 0.0 6.563425182726419 0.001014643479647339 70-71 0.3388909222022112 1.3136009334720013E-4 0.0 7.176120365706501 0.0010372917716037528 72-73 0.34550875311187534 1.4041941012976565E-4 0.0 7.73037389612225 0.0011007069890817114 74-75 0.35176421135023683 1.5400838530361393E-4 0.0 8.274616881493266 0.001127884939429408 76-77 0.3536757271913581 1.5853804369489672E-4 0.0 8.853729177160375 0.0011369442562119735 78-79 0.3550980399262209 1.6306770208617948E-4 0.0 9.457442047550542 0.0011595925481683873 80-81 0.35814197036516293 1.7665667726002776E-4 0.0 10.12602868542066 0.00116412220655967 82-83 0.35930609257172264 1.8118633565131052E-4 0.0 10.846398378019924 0.0012139484488637804 84-85 0.3637043908696582 1.8118633565131052E-4 0.0 11.535413775234726 0.0012139484488637804 86-87 0.37062117923314697 1.8118633565131052E-4 0.0 12.22855569124399 0.0012411263992114772 88 0.37222920796205233 1.8118633565131052E-4 0.0 12.766697226761947 0.0012683043495591738 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAAG 3200 0.0 21.8791 1 TCTACAC 10960 0.0 20.406473 3 ATCTACA 10315 0.0 19.222805 2 TACACTC 9645 0.0 19.09579 5 GTACATA 2800 0.0 17.788568 1 GTACTAG 2760 0.0 17.195131 1 CATGGGG 19250 0.0 16.890366 4 CTACACT 12995 0.0 16.885124 4 TAGTACT 3470 0.0 15.842368 4 GTATAAA 5945 0.0 15.49164 1 GTCCTAC 3320 0.0 15.427005 1 GTACAAG 5155 0.0 15.222325 1 GTACAAA 5395 0.0 15.154827 1 TATTCGC 695 0.0 14.871619 9 TGATCGC 2315 0.0 14.814679 9 TATAAGG 4270 0.0 14.745192 2 GTATTAG 4720 0.0 14.733737 1 GTGATCG 2395 0.0 14.320086 8 GTATAAT 2475 0.0 14.238993 1 ATAAGGT 3265 0.0 13.815277 3 >>END_MODULE