##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765477_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7041747 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18264104063949 33.0 31.0 34.0 31.0 34.0 2 32.18336444067076 34.0 31.0 34.0 30.0 34.0 3 32.2006726455807 34.0 31.0 34.0 30.0 34.0 4 35.67395065457478 37.0 35.0 37.0 35.0 37.0 5 35.70193816960479 37.0 35.0 37.0 35.0 37.0 6 35.816721617519065 37.0 36.0 37.0 35.0 37.0 7 35.77360476029599 37.0 36.0 37.0 35.0 37.0 8 35.81599566130394 37.0 36.0 37.0 35.0 37.0 9 37.49796648473738 39.0 38.0 39.0 35.0 39.0 10-11 37.430004940535355 39.0 38.0 39.0 35.0 39.0 12-13 37.41152508035293 39.0 38.0 39.0 35.0 39.0 14-15 38.771882886448495 41.0 38.5 41.0 35.0 41.0 16-17 38.71946840748468 40.0 38.0 41.0 35.0 41.0 18-19 38.69710662709126 40.0 38.5 41.0 34.5 41.0 20-21 38.68238172998831 40.0 39.0 41.0 34.5 41.0 22-23 38.60020411128091 40.0 38.5 41.0 34.0 41.0 24-25 38.524974058284116 40.0 38.0 41.0 34.0 41.0 26-27 38.38770045274276 40.0 38.0 41.0 34.0 41.0 28-29 38.25139123856623 40.0 38.0 41.0 34.0 41.0 30-31 38.03012576282562 40.0 38.0 41.0 33.5 41.0 32-33 37.747544607893474 40.0 37.0 41.0 33.0 41.0 34-35 37.61769259815782 40.0 37.0 41.0 33.0 41.0 36-37 37.485928704908034 40.0 37.0 41.0 33.0 41.0 38-39 37.304888687423734 40.0 37.0 41.0 32.0 41.0 40-41 37.04966082990485 40.0 36.5 41.0 31.0 41.0 42-43 36.793877073402385 40.0 36.0 41.0 31.0 41.0 44-45 36.44251525935255 39.5 35.5 41.0 30.0 41.0 46-47 36.01362992734615 39.0 35.0 41.0 29.5 41.0 48-49 35.61220148920431 39.0 35.0 41.0 27.5 41.0 50-51 34.36842242415128 38.0 34.0 40.0 25.5 40.5 52-53 34.31915588560623 38.0 34.0 39.5 25.5 40.0 54-55 35.14284551830675 39.0 35.0 40.5 27.0 41.0 56-57 35.16037419407428 39.0 35.0 41.0 27.0 41.0 58-59 34.960740282205535 38.0 35.0 41.0 26.5 41.0 60-61 34.61128857654215 37.5 35.0 40.0 26.0 41.0 62-63 34.14463733218476 37.0 34.0 40.0 25.5 41.0 64-65 33.871759948206034 36.5 34.0 39.5 25.0 41.0 66-67 33.583981574458726 36.0 34.0 39.0 25.0 41.0 68-69 33.36789137695518 35.5 34.0 39.0 24.5 41.0 70-71 32.98684580687151 35.0 34.0 38.5 24.0 40.5 72-73 32.594270285484555 35.0 34.0 37.0 23.5 39.0 74-75 32.218791089767926 35.0 33.0 37.0 21.5 39.0 76-77 31.834728157657466 35.0 33.0 36.0 20.0 38.5 78-79 31.510428165056197 35.0 33.0 36.0 19.5 37.0 80-81 31.206917118720682 35.0 33.0 35.0 17.5 37.0 82-83 30.946803115760904 35.0 33.0 35.0 13.5 36.5 84-85 30.686856897869234 35.0 33.0 35.0 7.5 36.0 86-87 30.41813246059536 35.0 32.0 35.0 2.0 36.0 88-89 30.19156183827678 35.0 32.0 35.0 2.0 35.5 90-91 29.93730994595517 35.0 32.0 35.0 2.0 35.0 92-93 29.621929259883945 35.0 31.0 35.0 2.0 35.0 94-95 29.33296524285806 35.0 31.0 35.0 2.0 35.0 96-97 29.06138945349783 35.0 31.0 35.0 2.0 35.0 98-99 28.725188152883085 35.0 31.0 35.0 2.0 35.0 100 28.14218801101488 34.0 29.0 35.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.502692397419267 1101 2 0.6229349933360524 1101 3 0.6583505177343838 1101 4 0.5176288671625784 1101 5 0.5029885311875191 1101 6 0.4951203361291405 1101 7 0.607884372481692 1101 8 0.5107206194985352 1101 9 0.6326339386165287 1101 10-11 0.6544359398958974 1101 12-13 0.6404652360576861 1101 14-15 0.820886257105677 1101 16-17 0.795251268600083 1101 18-19 0.8704523380253022 1101 20-21 0.8675373996217104 1101 22-23 0.8291961624533428 1101 24-25 0.8402127404651125 1101 26-27 0.9116313647059755 1101 28-29 0.8927453252647126 1101 30-31 0.9108672752760256 1101 32-33 0.9604011906199972 1101 34-35 1.1090651734420263 1101 36-37 1.1515347527677235 1101 38-39 1.1044838823888838 1101 40-41 1.177174275898885 1101 42-43 1.1925620745243677 1101 44-45 1.1821067942815375 1101 46-47 1.1554734139440868 1101 48-49 1.316265545092996 1101 50-51 1.1763919667175458 1101 52-53 1.2072692308348039 1101 54-55 1.3533949783781338 1101 56-57 1.428901899933031 1101 58-59 1.4293159290249164 1101 60-61 1.392342949995438 1101 62-63 1.4537719465136334 1101 64-65 1.4156045992425916 1101 66-67 1.3798033555242313 1101 68-69 1.3658604355366535 1101 70-71 1.3188578497874985 1101 72-73 1.321503595159136 1101 74-75 1.2659791098005826 1101 76-77 1.190438830059005 1101 78-79 1.2588325233394926 1101 80-81 1.2861786269936069 1101 82-83 1.241575667094036 1101 84-85 1.2358405606825613 1101 86-87 1.1691822698943923 1101 88-89 1.2115812815256355 1101 90-91 1.2515745720177058 1101 92-93 1.2665041941609694 1101 94-95 1.2155212757479639 1101 96-97 1.2699996667597127 1101 98-99 1.134033071294521 1101 100 1.2209070772609394 1102 1 0.43975370667096314 1102 2 0.5561940081293884 1102 3 0.6113861617769274 1102 4 0.4568254337945348 1102 5 0.4489643864371402 1102 6 0.4838370807340837 1102 7 0.5248088336933705 1102 8 0.4636596021884216 1102 9 0.5995087613210757 1102 10-11 0.6262766383416718 1102 12-13 0.6464640787539864 1102 14-15 0.7835445995558601 1102 16-17 0.7887088745474529 1102 18-19 0.7375272672800861 1102 20-21 0.7321623989677946 1102 22-23 0.7353755364259555 1102 24-25 0.7819480344983774 1102 26-27 0.7917188560553043 1102 28-29 0.8904129988767266 1102 30-31 0.9257765464126422 1102 32-33 0.9326452915858852 1102 34-35 0.8673217455449276 1102 36-37 0.9092532917165315 1102 38-39 0.889854959550398 1102 40-41 0.9974132697432978 1102 42-43 1.0305452779268265 1102 44-45 1.043627577062992 1102 46-47 1.0379571044502285 1102 48-49 1.1648636601628723 1102 50-51 1.232998005723303 1102 52-53 1.219639538316791 1102 54-55 1.2590452124460967 1102 56-57 1.231867655185063 1102 58-59 1.281598026272512 1102 60-61 1.2998253115767398 1102 62-63 1.2770677543843476 1102 64-65 1.2658947604905393 1102 66-67 1.2345442084290426 1102 68-69 1.1835240995925531 1102 70-71 1.141232674770201 1102 72-73 1.1715190275625957 1102 74-75 1.136576727726002 1102 76-77 1.136216485660075 1102 78-79 1.1343475344526723 1102 80-81 1.1856067927415062 1102 82-83 1.1184631242878815 1102 84-85 1.1404976702279903 1102 86-87 1.1553141839528642 1102 88-89 1.1319484427885982 1102 90-91 1.1871582012161177 1102 92-93 1.2498044942195428 1102 94-95 1.1838613752072327 1102 96-97 1.2375557933732573 1102 98-99 1.3506251072293232 1102 100 1.3413882982450254 1103 1 0.3678664629763162 1103 2 0.499430087813316 1103 3 0.5731363303300228 1103 4 0.40229840808847683 1103 5 0.4499423958123785 1103 6 0.42419015327268283 1103 7 0.5363778180118217 1103 8 0.498280662165989 1103 9 0.5918784434717068 1103 10-11 0.6015334699896542 1103 12-13 0.6529823690553442 1103 14-15 0.7553336745942048 1103 16-17 0.7561339397424547 1103 18-19 0.682001379479793 1103 20-21 0.7232424147576424 1103 22-23 0.7204038357566347 1103 24-25 0.7057060045730594 1103 26-27 0.6848491767544971 1103 28-29 0.7227845637474957 1103 30-31 0.7952526672761664 1103 32-33 0.8380717298428024 1103 34-35 0.8754089846185806 1103 36-37 0.8775011583194328 1103 38-39 0.8942986417955368 1103 40-41 0.9105185800498461 1103 42-43 0.923315555977986 1103 44-45 0.9163357927646203 1103 46-47 0.9477870976406919 1103 48-49 1.0557195023286567 1103 50-51 0.9494475436448298 1103 52-53 0.9824178117999125 1103 54-55 1.0851953892851185 1103 56-57 1.0637539078776115 1103 58-59 1.0633015748261343 1103 60-61 1.035357768672995 1103 62-63 1.1511718152437638 1103 64-65 1.086453712031151 1103 66-67 1.0236458297808113 1103 68-69 1.0192182181206988 1103 70-71 1.0493769071900445 1103 72-73 0.9408608667215432 1103 74-75 0.9327391471488582 1103 76-77 0.9779233118137292 1103 78-79 0.9667219185007383 1103 80-81 0.972862144076263 1103 82-83 0.9795589648553182 1103 84-85 0.987363652116489 1103 86-87 0.9317936284425592 1103 88-89 0.9493614529363477 1103 90-91 0.949003648768791 1103 92-93 0.9855939867412786 1103 94-95 0.9375479704543288 1103 96-97 0.9485990685414514 1103 98-99 0.8990117043211434 1103 100 0.9636098687878594 1104 1 0.3654995970063055 1104 2 0.44316123163305576 1104 3 0.4936965176872192 1104 4 0.4618743420909368 1104 5 0.45958749755921957 1104 6 0.4208256603542466 1104 7 0.4825075488492132 1104 8 0.4414773063329207 1104 9 0.5939438008838493 1104 10-11 0.5889183265518412 1104 12-13 0.5734085349862355 1104 14-15 0.6387263897287312 1104 16-17 0.682205183312206 1104 18-19 0.6679599784372527 1104 20-21 0.5839831297690878 1104 22-23 0.6084558176718389 1104 24-25 0.6694009483332266 1104 26-27 0.7286376246468009 1104 28-29 0.7444425055400075 1104 30-31 0.7002080817576442 1104 32-33 0.6949410495944548 1104 34-35 0.7401856478244824 1104 36-37 0.7348462937857576 1104 38-39 0.6998683660857026 1104 40-41 0.7560137630501558 1104 42-43 0.780504941100375 1104 44-45 0.8126287442526703 1104 46-47 0.8249424238742193 1104 48-49 0.7248001407210793 1104 50-51 0.6413637677235684 1104 52-53 0.6758813092801503 1104 54-55 0.7735705135126665 1104 56-57 0.639679662862342 1104 58-59 0.7124222991064713 1104 60-61 0.6820837917640148 1104 62-63 0.6951029285478612 1104 64-65 0.7439848755813827 1104 66-67 0.7068453478746548 1104 68-69 0.6983664033119794 1104 70-71 0.6559638303150379 1104 72-73 0.7061698408279753 1104 74-75 0.7216384299676903 1104 76-77 0.7012443246921585 1104 78-79 0.7001935080202344 1104 80-81 0.7296249921314946 1104 82-83 0.7793429286603377 1104 84-85 0.7552086446794881 1104 86-87 0.7221179000242124 1104 88-89 0.7308510162358566 1104 90-91 0.6957313054937622 1104 92-93 0.7238184201319946 1104 94-95 0.6881465466233863 1104 96-97 0.7722623991055322 1104 98-99 0.7722915115586808 1104 100 0.8679514016947998 1105 1 0.2517481893748936 1105 2 0.31381890572012594 1105 3 0.37988027454944273 1105 4 0.3342749581380602 1105 5 0.37315730502604083 1105 6 0.3517881823475051 1105 7 0.36124912414688026 1105 8 0.3243935405876428 1105 9 0.40197007209084745 1105 10-11 0.4005084595176598 1105 12-13 0.4375357081847895 1105 14-15 0.5057147596641087 1105 16-17 0.4984840098208494 1105 18-19 0.4932149712268412 1105 20-21 0.4748494587677641 1105 22-23 0.43351277595622406 1105 24-25 0.4591053946844923 1105 26-27 0.4448397746996662 1105 28-29 0.45065816520962443 1105 30-31 0.42682102677513 1105 32-33 0.4875635376508214 1105 34-35 0.4462890544525422 1105 36-37 0.4756371636006804 1105 38-39 0.4603703980421514 1105 40-41 0.48177754984745746 1105 42-43 0.5599132169894503 1105 44-45 0.5195644168230302 1105 46-47 0.47275656078092254 1105 48-49 0.5495801287315416 1105 50-51 0.462010684957626 1105 52-53 0.4804667660055273 1105 54-55 0.5343336748517729 1105 56-57 0.5617649657348025 1105 58-59 0.5779705614658326 1105 60-61 0.6341101898850496 1105 62-63 0.7006007224817878 1105 64-65 0.6434184282059263 1105 66-67 0.5707645334416256 1105 68-69 0.5564451363733838 1105 70-71 0.5502998986086283 1105 72-73 0.5002016191511913 1105 74-75 0.5567213640428079 1105 76-77 0.5483551680813044 1105 78-79 0.5676495113465378 1105 80-81 0.4982814850207795 1105 82-83 0.5195005600143858 1105 84-85 0.4645102992208656 1105 86-87 0.43825170994874796 1105 88-89 0.5000967996265793 1105 90-91 0.5435065083868409 1105 92-93 0.5771325845850264 1105 94-95 0.6274903253189379 1105 96-97 0.5919294357200968 1105 98-99 0.5692029092481867 1105 100 0.6089041383162446 1106 1 0.1718471062342033 1106 2 0.2993993119154723 1106 3 0.24952365124771347 1106 4 0.2390843711451609 1106 5 0.3388434122494459 1106 6 0.28515522373039204 1106 7 0.22774124904699988 1106 8 0.2441355257739204 1106 9 0.27666418127917325 1106 10-11 0.3686123059898634 1106 12-13 0.3825427168695015 1106 14-15 0.39981792122740245 1106 16-17 0.4105439031027345 1106 18-19 0.39904453061834033 1106 20-21 0.4273427706269146 1106 22-23 0.46838818583309205 1106 24-25 0.4266667781571982 1106 26-27 0.4530549810839304 1106 28-29 0.420247474138705 1106 30-31 0.5086995923183721 1106 32-33 0.5003928984944821 1106 34-35 0.4990223982091848 1106 36-37 0.4936099414472821 1106 38-39 0.4955327014351596 1106 40-41 0.5494359025712114 1106 42-43 0.5164260027671475 1106 44-45 0.4928116586580984 1106 46-47 0.4808700370939505 1106 48-49 0.5183087787522851 1106 50-51 0.5521352074554784 1106 52-53 0.5310687771274516 1106 54-55 0.5796015332022222 1106 56-57 0.5663885769142922 1106 58-59 0.5434015536478043 1106 60-61 0.5402071883502231 1106 62-63 0.5662128314115122 1106 64-65 0.5484347421300768 1106 66-67 0.532783835321446 1106 68-69 0.5339778196744263 1106 70-71 0.5244116188282106 1106 72-73 0.5193688110409198 1106 74-75 0.5917764516013193 1106 76-77 0.5909789642835754 1106 78-79 0.5415125652720221 1106 80-81 0.6210519361247258 1106 82-83 0.6323149134544295 1106 84-85 0.6273421432650537 1106 86-87 0.5733972314506133 1106 88-89 0.5420284158546984 1106 90-91 0.566623633760333 1106 92-93 0.5523151539239386 1106 94-95 0.49632722989857925 1106 96-97 0.5065138729235237 1106 98-99 0.52415667789721 1106 100 0.5352343195898825 1107 1 0.20904252562287695 1107 2 0.28051085866102454 1107 3 0.2681702425213359 1107 4 0.2834201672275043 1107 5 0.3539674747160291 1107 6 0.27635796517229494 1107 7 0.34600910991780864 1107 8 0.31553572842879873 1107 9 0.32753371328897174 1107 10-11 0.42452881014225596 1107 12-13 0.4091055271529669 1107 14-15 0.4119103583305659 1107 16-17 0.3980853124197665 1107 18-19 0.4100233333251566 1107 20-21 0.3648592808894975 1107 22-23 0.421092864716762 1107 24-25 0.42944238624914277 1107 26-27 0.47198523827232464 1107 28-29 0.43781215312420585 1107 30-31 0.4255780961346929 1107 32-33 0.3891263054896399 1107 34-35 0.3940949822625939 1107 36-37 0.4298376583211123 1107 38-39 0.4150363497321976 1107 40-41 0.43524695489995935 1107 42-43 0.36182955584694554 1107 44-45 0.4534677411050225 1107 46-47 0.30719780845036126 1107 48-49 0.31779404553878265 1107 50-51 0.28388440421910843 1107 52-53 0.32207757071989107 1107 54-55 0.3720514677721951 1107 56-57 0.3559050566258932 1107 58-59 0.337381430731007 1107 60-61 0.3703388121861053 1107 62-63 0.34087638866660797 1107 64-65 0.3659637273790395 1107 66-67 0.40224537417466166 1107 68-69 0.3872716234250717 1107 70-71 0.36265060901624935 1107 72-73 0.3292664652342978 1107 74-75 0.37996528725228274 1107 76-77 0.4205201902946847 1107 78-79 0.4474722481527813 1107 80-81 0.4876037480528481 1107 82-83 0.5070665084705439 1107 84-85 0.5522409431204416 1107 86-87 0.5243004713827908 1107 88-89 0.5081469129498082 1107 90-91 0.4486721792787627 1107 92-93 0.4556517290564521 1107 94-95 0.36750675259032306 1107 96-97 0.4155292655291696 1107 98-99 0.43189957097384735 1107 100 0.4365995413645898 1108 1 0.20707180435445594 1108 2 0.21122901439274955 1108 3 0.2499135429444692 1108 4 0.28327309266887113 1108 5 0.34419732734966146 1108 6 0.3269348826374028 1108 7 0.3465322362248955 1108 8 0.32784621916368906 1108 9 0.4284435912131954 1108 10-11 0.4047611918357532 1108 12-13 0.39982368288469416 1108 14-15 0.42789858847500994 1108 16-17 0.40975167105991517 1108 18-19 0.46034267576959564 1108 20-21 0.39552452090700996 1108 22-23 0.371071367707863 1108 24-25 0.48160988782979786 1108 26-27 0.5117969205834925 1108 28-29 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-0.7769313772807678 2316 82-83 -0.7709577014839901 2316 84-85 -0.679797647073979 2316 86-87 -0.6893289962976041 2316 88-89 -0.6917916050066388 2316 90-91 -0.6805205037215636 2316 92-93 -0.5704668195386944 2316 94-95 -0.5253829872482179 2316 96-97 -0.5523710973043201 2316 98-99 -0.5838711009231297 2316 100 -0.6647580749723119 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 19449.0 3 3329.0 4 3331.0 5 3874.0 6 4616.0 7 5576.0 8 6893.0 9 8350.0 10 10705.0 11 14968.0 12 21786.0 13 29456.0 14 31675.0 15 38711.0 16 50418.0 17 67061.0 18 67012.0 19 40032.0 20 34689.0 21 32555.0 22 34413.0 23 39665.0 24 45127.0 25 50312.0 26 57267.0 27 67173.0 28 80475.0 29 97155.0 30 117475.0 31 146241.0 32 184320.0 33 243398.0 34 349443.0 35 553450.0 36 995161.0 37 1748633.0 38 1466370.0 39 270922.0 40 261.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.185363905502534 17.53385171598439 12.92904585924903 24.351738519264046 2 14.753408754624974 21.86620655915493 36.55371529023094 26.82666939598915 3 19.63960087823781 27.16117653985651 29.049550352904646 24.149672229001037 4 13.784786030974328 18.377134440624413 33.936132253384535 33.901947275016724 5 13.634408810467008 36.47675337749431 33.54391494037755 16.344922871661126 6 31.419422284170018 33.0348006968985 18.416229754084597 17.129547264846888 7 25.224897711703388 32.021502907964496 22.476031747454048 20.277567632878068 8 27.672743664086752 30.88898305446262 21.567202699455244 19.871070581995383 9 27.997552228610463 14.53095292309444 19.70327887516366 37.76821597313144 10-11 24.88722024936425 25.682196702913394 25.92247140178196 23.508111645940392 12-13 27.438797962216487 23.536768191948926 26.145214207665983 22.879219638168603 14-15 26.164461320499644 22.871027749201414 24.877749820114698 26.08676111018425 16-17 23.28439113955067 26.055603140745802 27.620024536987724 23.039981182715803 18-19 24.641760960809584 25.11372831595044 28.84125627918415 21.403254444055825 20-21 24.083060796067212 24.76762443608504 28.262819753313295 22.886495014534454 22-23 23.026403805239852 25.589735860951734 25.898868568883554 25.484991764924857 24-25 23.117248482817956 26.632366242037087 25.30472214130822 24.945663133836742 26-27 24.238047938056155 24.613503556571736 25.872765739977105 25.275682765395008 28-29 23.445955174150445 24.109287549544884 28.366683359463252 24.07807391684142 30-31 25.092697395833518 24.493134560758843 27.227582310014192 23.186585733393443 32-33 22.034023864440822 25.476232766966316 27.71932738472907 24.770415983863792 34-35 21.751164518133407 26.12631707528817 28.284695523115417 23.837822883463005 36-37 24.00179073553773 25.587994651957942 26.71117019265403 23.6990444198503 38-39 24.78379064470086 24.365862644271047 26.336613440727096 24.513733270300992 40-41 23.321767846553833 25.612741962718683 26.574857709121112 24.49063248160637 42-43 25.386891831452154 24.77163678648443 26.18789872871546 23.65357265334796 44-45 25.3315480944105 24.317222874067873 26.45952379666244 23.891705234859185 46-47 23.70526361000935 24.827263118918566 28.479432604545412 22.988040666526675 48-49 24.531802780890754 25.524415828547152 27.331823305540365 22.611958085021726 50-51 22.767469196610733 26.184872691844323 26.469495667237464 24.578162444307484 52-53 22.406569894268618 26.58822596092637 24.64716219531328 26.35804194949174 54-55 23.31454026321666 26.307058346333456 24.33916081208287 26.039240578367018 56-57 24.232192648778586 24.496735600518825 24.89266177549763 26.37840997520496 58-59 23.779424815184566 24.28817019142213 26.933542362890815 24.998862630502487 60-61 24.205496504454675 25.009992145576604 26.035707920061697 24.748803429907017 62-63 21.890582694975453 25.456353208534598 26.813987567888827 25.83907652860112 64-65 21.60473099287673 25.70489922984035 27.03903797003352 25.6513318072494 66-67 23.939289625903154 25.160991654143178 25.741371196334722 25.158347523618946 68-69 24.454680761332565 24.243499572364133 25.523145000611926 25.77867466569138 70-71 23.00103772023472 25.23925295518493 25.891517995018713 25.86819132956163 72-73 24.70323687287188 24.568132490193236 25.766719860728276 24.961910776206608 74-75 25.0954857257342 24.029248613212303 25.986951446209254 24.888314214844236 76-77 23.683544746885712 25.062003985899377 27.35637201765452 23.898079249560386 78-79 24.126178119382764 25.155231730148653 26.904251630831926 23.814338519636657 80-81 22.866467040844263 25.33854941790964 26.685789182964086 25.10919435828201 82-83 22.79173919615733 25.986615562675507 24.938628710202515 26.283016530964648 84-85 23.368582373516926 26.00784723391299 24.55935143643697 26.06421895613311 86-87 23.94654777772854 24.44001758068418 25.270078245994572 26.34335639559271 88-89 23.97638275772896 23.959334679730837 26.439598880132596 25.624683682407607 90-91 24.698441465020604 24.785947575722915 25.577123465495234 24.93848749376125 92-93 23.215279671725114 25.119445735112546 26.150667630207565 25.514606962954783 94-95 22.699521621513394 25.451954306297043 26.56644407700057 25.282079995188994 96-97 24.72979219256605 24.8663149424506 25.2192932297043 25.18459963527905 98-99 25.104136698288563 24.287359690964422 24.93533245760327 25.67317115314375 100 24.475178426290174 24.975859825745935 25.619407716387276 24.929554031576618 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1735.0 1 2129.0 2 1772.5 3 944.0 4 772.5 5 781.5 6 1027.5 7 1688.0 8 2482.0 9 2608.0 10 2129.5 11 1871.5 12 2061.5 13 2319.0 14 2639.0 15 3076.0 16 3586.5 17 4143.5 18 4764.5 19 5526.5 20 6481.5 21 7645.0 22 9258.5 23 11288.0 24 13544.0 25 16544.0 26 20724.5 27 25601.0 28 30637.0 29 37155.5 30 44830.5 31 51241.0 32 58150.5 33 66033.0 34 73618.0 35 79687.5 36 85426.0 37 93630.5 38 100346.5 39 106543.5 40 111042.5 41 115157.5 42 123379.0 43 139640.5 44 160226.0 45 179249.5 46 207227.5 47 246613.0 48 345357.5 49 496879.5 50 705974.5 51 655829.5 52 388278.0 53 290320.5 54 257951.5 55 229607.0 56 209076.5 57 186168.5 58 171534.0 59 156914.5 60 133512.0 61 111164.0 62 90830.5 63 71864.5 64 54450.0 65 43351.5 66 34114.0 67 25365.0 68 19761.5 69 16262.0 70 13915.5 71 12018.5 72 10179.5 73 9758.5 74 8198.0 75 6646.0 76 5180.0 77 3571.0 78 2611.0 79 1817.0 80 1295.5 81 1045.5 82 806.5 83 572.0 84 406.0 85 249.0 86 174.0 87 130.5 88 90.0 89 74.0 90 62.5 91 49.0 92 40.0 93 40.5 94 40.0 95 26.5 96 23.5 97 21.5 98 12.0 99 9.0 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.11650517975155882 2 0.37739214430737145 3 0.3869210296819809 4 0.3830015477693249 5 0.3099230915282813 6 0.1422942346551218 7 0.15456391716430598 8 0.13089081445272033 9 0.21226266720460138 10-11 0.21298691929715735 12-13 0.2254838181491042 14-15 0.15447871103576996 16-17 0.14078892638431914 18-19 0.15871061541972467 20-21 0.15919345014809536 22-23 0.16695430835558278 24-25 0.1800618511287043 26-27 0.1726063148818042 28-29 0.18130444050318764 30-31 0.2242980328603115 32-33 0.2209820943581188 34-35 0.13557005101148906 36-37 0.09442259143931186 38-39 0.08811733792764778 40-41 0.08746409094220511 42-43 0.07779319535336898 44-45 0.09970537140854394 46-47 0.14854978459180657 48-49 0.22790509230166892 50-51 0.18425825295910234 52-53 0.11398449844903544 54-55 0.061241904885250775 56-57 0.07754467747847232 58-59 0.11322474380292277 60-61 0.08834455427041045 62-63 0.09798704781640123 64-65 0.10822598426214404 66-67 0.10364615485333399 68-69 0.09310189644700385 70-71 0.12813581629672297 72-73 0.15460652022857396 74-75 0.19874329481022251 76-77 0.19843087233892384 78-79 0.15918634963738404 80-81 0.09351372606826118 82-83 0.13561975458646838 84-85 0.1651081755706361 86-87 0.17668200803010958 88-89 0.16514367812419276 90-91 0.18596237552982237 92-93 0.10333373238203532 94-95 0.3479250248553377 96-97 0.3493664285297384 98-99 0.3892357961738756 100 0.39061329525187427 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7041747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.02015343205315 #Duplication Level Percentage of deduplicated Percentage of total 1 77.14499466240845 26.24484554930057 2 12.035503314017584 8.188993387497248 3 3.8199955814665225 3.898705073737686 4 1.8558848981386462 2.525499559476283 5 1.0968921798075182 1.8658220127735503 6 0.6873241995327967 1.4029724835401318 7 0.4761206818995332 1.1338389055277125 8 0.3716554140456781 1.0115019367749767 9 0.2931042939473731 0.8974307746514917 >10 1.8447173072812046 12.03125064219514 >50 0.1904841466450963 4.482749948951962 >100 0.1534646026912844 10.666253185781345 >500 0.01874066985568742 4.3076010824604465 >1k 0.008970243484579346 5.375631785574097 >5k 7.15934925997715E-4 1.8435363184820586 >10k+ 0.00143186985199543 14.1233673532752 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 132408 1.880328844532472 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 123675 1.7563113244483224 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 57939 0.8227929802078945 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 46831 0.6650480342449111 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 45370 0.6443003419463949 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 44656 0.6341608126506108 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 40442 0.5743177083754926 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 40127 0.5698443866273525 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 33518 0.4759898360449473 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 30939 0.4393654017958896 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 29814 0.4233892526953894 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 29793 0.4230910312455134 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 26457 0.37571642377949677 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21727 0.30854559245028257 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 20716 0.2941883597919664 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 19450 0.27620986667087016 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 19292 0.2739661052860888 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 16763 0.23805172210816433 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 16730 0.23758308840121636 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 15455 0.21947678608731613 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 15418 0.21895134829467744 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14893 0.21149581204777732 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 13784 0.1957468792900398 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 13307 0.18897299207142773 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 13258 0.18827714202171705 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 12575 0.1785778443900356 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 12398 0.17606426359822355 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 12382 0.1758370472554609 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 11875 0.1686371293941688 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 11692 0.16603834247382077 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 10794 0.15328582523626594 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10551 0.1498349770305579 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 10545 0.1497497709020219 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 10200 0.14485041851120184 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 9999 0.1419960132052458 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 9834 0.13965284467050576 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 9517 0.1351511208795204 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 9299 0.132055298209379 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8215 0.11666139098720815 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8141 0.1156105154019308 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8059 0.11444603164527212 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 7976 0.11326734686719078 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 7681 0.10907804554750404 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 7479 0.10620943922012534 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 7400 0.10508755852773467 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 1.2780919280400163E-4 0.0 0.0 0.0 0.0 2 1.84613278494669E-4 0.0 0.0 0.0 0.0 3 2.130153213400027E-4 0.0 0.0 0.0 0.0 4 3.408245141440043E-4 0.0 0.0 1.4201021422666846E-5 0.0 5 0.001491107249380019 0.0 0.0 4.260306426800054E-5 0.0 6 0.002669792027461367 0.0 0.0 5.6804085690667385E-5 0.0 7 0.00288280734880137 0.0 0.0 5.6804085690667385E-5 0.0 8 0.003266234927213375 0.0 0.0 8.520612853600108E-5 0.0 9 0.003536054334244045 0.0 0.0 1.2780919280400163E-4 0.0 10-11 0.005815318272582074 0.0 0.0 1.84613278494669E-4 0.0 12-13 0.012099270252112153 1.4201021422666846E-5 0.0 2.2011583205133612E-4 0.0 14-15 0.019370193220517577 1.4201021422666846E-5 0.0 3.1242247129867063E-4 0.0 16-17 0.022253000569318948 1.4201021422666846E-5 0.0 4.1182962125733855E-4 0.0 18-19 0.023886118032925636 1.4201021422666846E-5 0.0 5.467393247726737E-4 7.100510711333423E-6 20-21 0.03057479912300172 1.4201021422666846E-5 0.0 9.017648603393448E-4 1.4201021422666846E-5 22-23 0.03260554518644308 1.4201021422666846E-5 7.100510711333423E-6 0.002549083345368699 1.4201021422666846E-5 24-25 0.04697697886618193 1.4201021422666846E-5 1.4201021422666846E-5 0.0064969673008700825 1.4201021422666846E-5 26-27 0.0919019101367885 1.4201021422666846E-5 1.4201021422666846E-5 0.012930030005338164 1.4201021422666846E-5 28-29 0.10111837304009928 1.4201021422666846E-5 2.8402042845333692E-5 0.03828595375550982 1.4201021422666846E-5 30-31 0.10302841042144797 1.4201021422666846E-5 2.8402042845333692E-5 0.10636565045577469 1.4201021422666846E-5 32-33 0.10437040694588999 1.4201021422666846E-5 2.8402042845333692E-5 0.22190516075059213 1.4201021422666846E-5 34-35 0.10549228763828067 1.4201021422666846E-5 2.8402042845333692E-5 0.38350568402982954 1.4201021422666846E-5 36-37 0.1072603148054027 2.130153213400027E-5 2.8402042845333692E-5 0.6237159613942392 2.130153213400027E-5 38-39 0.1091916537188854 2.8402042845333692E-5 2.8402042845333692E-5 0.9637452183385742 2.8402042845333692E-5 40-41 0.11070406250039941 2.8402042845333692E-5 2.8402042845333692E-5 1.4197116141775612 2.8402042845333692E-5 42-43 0.11475845411657079 2.8402042845333692E-5 2.8402042845333692E-5 1.760884053346421 2.8402042845333692E-5 44-45 0.11902586105408217 2.8402042845333692E-5 2.8402042845333692E-5 2.127476320861854 2.8402042845333692E-5 46-47 0.12124832090672955 2.8402042845333692E-5 2.8402042845333692E-5 2.5544584319771784 2.8402042845333692E-5 48-49 0.12244830721694488 2.8402042845333692E-5 2.8402042845333692E-5 2.9357771587079173 2.8402042845333692E-5 50-51 0.1242163343840669 2.8402042845333692E-5 2.8402042845333692E-5 3.3440636251203006 2.8402042845333692E-5 52-53 0.1251251997551176 2.8402042845333692E-5 2.8402042845333692E-5 3.8092109813090413 3.550255355666712E-5 54-55 0.1272340514363836 2.8402042845333692E-5 2.8402042845333692E-5 4.246716049298562 4.260306426800054E-5 56-57 0.13188488595230702 2.8402042845333692E-5 2.8402042845333692E-5 4.711749797315922 4.260306426800054E-5 58-59 0.1335819080123157 2.8402042845333692E-5 2.8402042845333692E-5 5.193838972061904 4.260306426800054E-5 60-61 0.1346682861511497 2.8402042845333692E-5 2.8402042845333692E-5 5.624137021679422 4.260306426800054E-5 62-63 0.13530023160445837 2.8402042845333692E-5 2.8402042845333692E-5 6.063665735221671 4.260306426800054E-5 64-65 0.1357688653114064 2.8402042845333692E-5 2.8402042845333692E-5 6.553629376346523 4.260306426800054E-5 66-67 0.13668483119316838 2.8402042845333692E-5 2.8402042845333692E-5 7.060691047264266 4.260306426800054E-5 68-69 0.1376576011606211 2.8402042845333692E-5 2.8402042845333692E-5 7.6541162299639565 4.260306426800054E-5 70-71 0.1389356930886611 2.8402042845333692E-5 2.8402042845333692E-5 8.367845365645769 5.6804085690667385E-5 72-73 0.1422090285265858 2.8402042845333692E-5 2.8402042845333692E-5 8.95322566970952 5.6804085690667385E-5 74-75 0.14516994149321183 2.8402042845333692E-5 2.8402042845333692E-5 9.515344700682942 5.6804085690667385E-5 76-77 0.14610720890710785 2.8402042845333692E-5 2.8402042845333692E-5 10.138989656970068 6.390459640200081E-5 78-79 0.14672495333899388 3.550255355666712E-5 2.8402042845333692E-5 10.752544787536387 7.100510711333424E-5 80-81 0.14796754271347723 4.260306426800054E-5 2.8402042845333692E-5 11.418139561106072 7.100510711333424E-5 82-83 0.14864209123105387 4.970357497933396E-5 2.8402042845333692E-5 12.180045661964282 8.520612853600108E-5 84-85 0.15077934495516523 5.6804085690667385E-5 2.8402042845333692E-5 12.899320296511647 8.520612853600108E-5 86-87 0.15489764116773863 5.6804085690667385E-5 2.8402042845333692E-5 13.61384468939313 8.520612853600108E-5 88 0.1559556172637273 5.6804085690667385E-5 2.8402042845333692E-5 14.197698383653943 8.520612853600108E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCTAC 6325 0.0 26.142208 1 ATCTACA 7305 0.0 22.950834 2 TCTACAC 8245 0.0 22.734364 3 TACACTC 8215 0.0 22.50906 5 CTACACT 9865 0.0 21.047411 4 GTATAAG 1945 0.0 20.34489 1 AGTACTT 25565 0.0 19.912611 12-13 TATACCG 1190 0.0 19.769487 5 GAGTACT 24570 0.0 19.226774 12-13 CACTCTT 10220 0.0 18.313679 7 TACTTTT 30045 0.0 17.098467 14-15 CATGGGG 13890 0.0 16.846563 4 ACTCTTT 12050 0.0 16.817661 8 GTACTTT 28340 0.0 16.742954 14-15 ACACTCT 11175 0.0 16.620832 6 ATAAGGT 1965 0.0 16.058413 3 GTGATCG 1475 0.0 15.938717 8 ACTTTTT 31345 0.0 15.400889 16-17 TAGTACT 1810 0.0 15.347252 4 ATACCGA 1165 0.0 15.337723 6 >>END_MODULE