##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765477_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7041747 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51994881383838 34.0 31.0 34.0 31.0 34.0 2 32.65916838534528 34.0 31.0 34.0 31.0 34.0 3 32.702940051666154 34.0 31.0 34.0 31.0 34.0 4 36.19068506721415 37.0 35.0 37.0 35.0 37.0 5 36.13237865546717 37.0 35.0 37.0 35.0 37.0 6 36.19449150899628 37.0 37.0 37.0 35.0 37.0 7 36.15324080799836 37.0 36.0 37.0 35.0 37.0 8 36.171800264905855 37.0 37.0 37.0 35.0 37.0 9 37.91036400484141 39.0 38.0 39.0 35.0 39.0 10-11 37.86655960516616 39.0 38.0 39.0 35.0 39.0 12-13 37.83329662369296 39.0 38.0 39.0 35.0 39.0 14-15 39.20687210148277 41.0 39.0 41.0 36.0 41.0 16-17 39.17432371540755 40.0 39.0 41.0 36.0 41.0 18-19 39.204930182808326 40.0 39.0 41.0 36.0 41.0 20-21 39.191386242646885 40.5 39.0 41.0 36.0 41.0 22-23 39.13936009061388 40.0 39.0 41.0 36.0 41.0 24-25 39.08019252892784 40.0 39.0 41.0 35.0 41.0 26-27 38.936307353842736 40.0 38.0 41.0 35.0 41.0 28-29 38.81263577064044 40.0 38.0 41.0 35.0 41.0 30-31 38.64183312748953 40.0 38.0 41.0 35.0 41.0 32-33 38.36584287961496 40.0 38.0 41.0 34.0 41.0 34-35 38.27877364807341 40.0 38.0 41.0 34.0 41.0 36-37 38.181504816915464 40.0 38.0 41.0 34.0 41.0 38-39 38.02190401046786 40.0 37.5 41.0 33.5 41.0 40-41 37.79395297786189 40.0 37.0 41.0 33.0 41.0 42-43 37.53366359228754 40.0 37.0 41.0 33.0 41.0 44-45 37.2082907835229 40.0 36.5 41.0 32.5 41.0 46-47 36.85497029359334 40.0 36.0 41.0 31.5 41.0 48-49 36.541079010648915 39.0 35.5 41.0 31.0 41.0 50-51 36.26632652380155 39.0 35.0 41.0 30.5 41.0 52-53 36.1306889469332 39.0 35.0 41.0 30.0 41.0 54-55 36.11704666469841 39.0 35.0 41.0 30.5 41.0 56-57 35.9345212913784 39.0 35.0 41.0 30.0 41.0 58-59 35.8076074729751 39.0 35.0 41.0 30.0 41.0 60-61 35.48053821019131 38.0 35.0 40.5 30.0 41.0 62-63 35.113788879378944 37.0 35.0 40.0 29.0 41.0 64-65 34.89663573542191 37.0 35.0 40.0 29.0 41.0 66-67 34.5358323012741 36.0 35.0 39.0 29.0 41.0 68-69 34.20049399673121 36.0 34.5 39.0 29.0 41.0 70-71 33.803071808742914 35.0 34.0 38.5 29.0 40.5 72-73 33.41338910642487 35.0 34.0 37.0 28.0 39.0 74-75 33.070512047649544 35.0 34.0 37.0 27.5 39.0 76-77 31.623093459620176 34.5 32.0 35.0 25.0 37.0 78-79 32.36074158869951 35.0 33.5 36.0 26.0 37.0 80-81 32.326467352490795 35.0 34.0 35.0 27.0 37.0 82-83 32.17474768690213 35.0 34.0 35.0 26.5 36.5 84-85 31.998759398768517 35.0 34.0 35.0 26.0 36.0 86-87 31.824901334853408 35.0 33.0 35.0 26.0 36.0 88-89 31.68269912281711 35.0 33.0 35.0 25.5 35.5 90-91 31.55546024303344 35.0 33.0 35.0 25.0 35.0 92-93 31.388570691335545 35.0 33.0 35.0 25.0 35.0 94-95 31.305208636436383 35.0 33.0 35.0 24.0 35.0 96-97 31.21031258294284 35.0 33.0 35.0 24.0 35.0 98-99 31.07538079683919 35.0 33.0 35.0 23.0 35.0 100 30.74355823916991 35.0 33.0 35.0 19.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.46445440833387863 1101 2 0.38631812517352415 1101 3 0.4051376552053654 1101 4 0.29107419989237826 1101 5 0.32545548550567815 1101 6 0.31227272645158166 1101 7 0.2808858496432407 1101 8 0.3033517213103565 1101 9 0.3831780981878907 1101 10-11 0.40333375275553607 1101 12-13 0.462118259426866 1101 14-15 0.5734733243842385 1101 16-17 0.5708228702755704 1101 18-19 0.5196367098504311 1101 20-21 0.5246457601220129 1101 22-23 0.5101679914969353 1101 24-25 0.5674675406423262 1101 26-27 0.558472280463512 1101 28-29 0.6002068986230356 1101 30-31 0.6444197317607134 1101 32-33 0.726267473027896 1101 34-35 0.5993904954183975 1101 36-37 0.6819332005968946 1101 38-39 0.7474433583822773 1101 40-41 0.7767452765162943 1101 42-43 0.8042454192969686 1101 44-45 0.9056108033343904 1101 46-47 0.9429448058322691 1101 48-49 0.9316285697804219 1101 50-51 0.9963482683907117 1101 52-53 0.9727849085771751 1101 54-55 1.0557440542827337 1101 56-57 1.0764560207242582 1101 58-59 1.1026516428311481 1101 60-61 1.1146712110411485 1101 62-63 1.114645421678027 1101 64-65 1.1021126104427452 1101 66-67 1.1340489335668806 1101 68-69 1.1249578199954087 1101 70-71 1.127474210578761 1101 72-73 1.0958464434096058 1101 74-75 1.0716792012529552 1101 76-77 1.1003522591620936 1101 78-79 1.1529119972460933 1101 80-81 1.1646529444418263 1101 82-83 1.1165624941853878 1101 84-85 1.1285960899830911 1101 86-87 1.1402379220746077 1101 88-89 1.1091418661827035 1101 90-91 1.1143071336934405 1101 92-93 1.279818443109761 1101 94-95 1.234834406706316 1101 96-97 1.2638899182569574 1101 98-99 1.2491463115298984 1101 100 1.370808065979272 1102 1 0.42318703084851705 1102 2 0.3753955610758908 1102 3 0.3925820407552365 1102 4 0.2778711163817249 1102 5 0.2997256779491835 1102 6 0.2983427252686326 1102 7 0.2801913999252861 1102 8 0.2830574610675569 1102 9 0.36844034695148054 1102 10-11 0.3597762032220757 1102 12-13 0.3854436495865414 1102 14-15 0.4403426795122769 1102 16-17 0.47743234339188234 1102 18-19 0.49023893471532176 1102 20-21 0.4450305222562889 1102 22-23 0.49411665500354474 1102 24-25 0.47783928015685717 1102 26-27 0.5224599696412398 1102 28-29 0.6179557994967197 1102 30-31 0.6217542308353643 1102 32-33 0.6507343885367973 1102 34-35 0.6767509654814816 1102 36-37 0.7317392619454708 1102 38-39 0.6816918526471412 1102 40-41 0.757788598116278 1102 42-43 0.7430633900059433 1102 44-45 0.7624522687596382 1102 46-47 0.891215220691393 1102 48-49 0.904449668820682 1102 50-51 0.9034714236953647 1102 52-53 0.8829390756720485 1102 54-55 1.0314270583717757 1102 56-57 1.0138238011396368 1102 58-59 1.0106383208447198 1102 60-61 1.1138002126026194 1102 62-63 1.0092135531658144 1102 64-65 1.0364094016334704 1102 66-67 1.067159615034484 1102 68-69 0.9891512689274862 1102 70-71 0.9512589952920933 1102 72-73 1.0161378854879715 1102 74-75 1.0253672250497345 1102 76-77 1.0373748059963646 1102 78-79 1.0059346811072771 1102 80-81 1.053509956009627 1102 82-83 1.0744811396844298 1102 84-85 1.016878499760061 1102 86-87 1.0544734831584925 1102 88-89 1.1183916556219415 1102 90-91 1.1009360403316997 1102 92-93 1.1145315970019567 1102 94-95 1.138735927553224 1102 96-97 1.2032096909880252 1102 98-99 1.1993182279981482 1102 100 1.1872988504971858 1103 1 0.3876463368516383 1103 2 0.30156366726964734 1103 3 0.30280690262627274 1103 4 0.2636733718093609 1103 5 0.2941983437883593 1103 6 0.3016115613412538 1103 7 0.29231953128816457 1103 8 0.2492184776432751 1103 9 0.32613497575071193 1103 10-11 0.3525298910277286 1103 12-13 0.3259550250588532 1103 14-15 0.448664553809202 1103 16-17 0.4681349724646964 1103 18-19 0.43872256053523273 1103 20-21 0.46144919631507264 1103 22-23 0.47650923806677525 1103 24-25 0.5101934611224195 1103 26-27 0.550849651893607 1103 28-29 0.5726772227988235 1103 30-31 0.5701442569047899 1103 32-33 0.583027111437346 1103 34-35 0.5472487163602224 1103 36-37 0.6697673712268326 1103 38-39 0.6804623849145557 1103 40-41 0.709520712903128 1103 42-43 0.7018721607361726 1103 44-45 0.7443599686498814 1103 46-47 0.7328561179325632 1103 48-49 0.7637433349811786 1103 50-51 0.878887048568771 1103 52-53 0.9540420977872088 1103 54-55 0.9602861152576878 1103 56-57 0.9647166284600672 1103 58-59 0.9155790179181764 1103 60-61 0.9443403387804139 1103 62-63 0.9374019369664097 1103 64-65 0.937758221688263 1103 66-67 0.8797049172217868 1103 68-69 0.8526561424576542 1103 70-71 0.7731645789607668 1103 72-73 0.8196531176561095 1103 74-75 0.8064473370593248 1103 76-77 0.7983539081092275 1103 78-79 0.8900063263307345 1103 80-81 0.8639429283227926 1103 82-83 0.8708083430844624 1103 84-85 0.8484335604069955 1103 86-87 0.8067214093318391 1103 88-89 0.8489034337463153 1103 90-91 0.876045671088729 1103 92-93 0.8789337012849217 1103 94-95 0.9426494757654744 1103 96-97 0.9972157002482902 1103 98-99 0.9363422750288919 1103 100 0.9812772655275168 1104 1 0.3143175840375889 1104 2 0.2310282699111852 1104 3 0.24584779644926869 1104 4 0.2150118572752575 1104 5 0.22288102260545628 1104 6 0.234974204922608 1104 7 0.2208581965489529 1104 8 0.19213946590617326 1104 9 0.28519626747618076 1104 10-11 0.2533658516568309 1104 12-13 0.315708095735566 1104 14-15 0.40495374318100374 1104 16-17 0.39426926161664255 1104 18-19 0.3092080606857479 1104 20-21 0.3595589708069795 1104 22-23 0.37378054596680954 1104 24-25 0.3732960460388739 1104 26-27 0.4481905874932224 1104 28-29 0.4617928114415477 1104 30-31 0.44569862186369136 1104 32-33 0.42177895482672767 1104 34-35 0.4351172713174094 1104 36-37 0.39442875697348256 1104 38-39 0.45586650313128985 1104 40-41 0.40825521756787 1104 42-43 0.42977020357925255 1104 44-45 0.37328961168342545 1104 46-47 0.3818479754568429 1104 48-49 0.4173253867048814 1104 50-51 0.3340935192818364 1104 52-53 0.32116223269048305 1104 54-55 0.2934424451451605 1104 56-57 0.32944047612163274 1104 58-59 0.32277433487647045 1104 60-61 0.3914467606747891 1104 62-63 0.38938681808068054 1104 64-65 0.3440894573087121 1104 66-67 0.38276012992568553 1104 68-69 0.39784158528824065 1104 70-71 0.3587229058785226 1104 72-73 0.35991585040532925 1104 74-75 0.39770664996240157 1104 76-77 0.301723952211006 1104 78-79 0.393025535645549 1104 80-81 0.39048710099188355 1104 82-83 0.3758285953873255 1104 84-85 0.3288919676107227 1104 86-87 0.3839654910566743 1104 88-89 0.3885303973981209 1104 90-91 0.3752091870623424 1104 92-93 0.43504593479243425 1104 94-95 0.4688367073459325 1104 96-97 0.5179671338017329 1104 98-99 0.45262762298182224 1104 100 0.5014978473486309 1105 1 0.2070797266064517 1105 2 0.16128185360910408 1105 3 0.18299160711631401 1105 4 0.14946091738470813 1105 5 0.14222971805198625 1105 6 0.12873210404846702 1105 7 0.1524290040946923 1105 8 0.1312665187793698 1105 9 0.1764831978390191 1105 10-11 0.11896948704325183 1105 12-13 0.15091856300400508 1105 14-15 0.1806102277993844 1105 16-17 0.2572728296488975 1105 18-19 0.2052100510306616 1105 20-21 0.2199585463084759 1105 22-23 0.18829629062116737 1105 24-25 0.24671280386075267 1105 26-27 0.2336090797758814 1105 28-29 0.2922585112157279 1105 30-31 0.24442357964584716 1105 32-33 0.21428848891714125 1105 34-35 0.19487065761165923 1105 36-37 0.2678255384501611 1105 38-39 0.30517183565660133 1105 40-41 0.2521926491313593 1105 42-43 0.2749427106516933 1105 44-45 0.3035091304558293 1105 46-47 0.3482965004338894 1105 48-49 0.29697814034944514 1105 50-51 0.24785414173260278 1105 52-53 0.2806116888222192 1105 54-55 0.3302373192944188 1105 56-57 0.3822311925743094 1105 58-59 0.28390900016241005 1105 60-61 0.373705394673145 1105 62-63 0.3254906789077481 1105 64-65 0.2979333874272996 1105 66-67 0.39123582450788064 1105 68-69 0.33345739246038875 1105 70-71 0.32741931874840446 1105 72-73 0.31944314419092024 1105 74-75 0.34134059995499655 1105 76-77 0.27961676180615314 1105 78-79 0.31241928884848846 1105 80-81 0.33009865616974565 1105 82-83 0.35911463267902377 1105 84-85 0.34200527002955283 1105 86-87 0.3169119444984041 1105 88-89 0.3585597231401607 1105 90-91 0.3788162841741567 1105 92-93 0.37148641455929265 1105 94-95 0.3645609564000729 1105 96-97 0.3599316617938726 1105 98-99 0.3832739462992727 1105 100 0.4360619291509913 1106 1 0.19673861925049607 1106 2 0.07688054027193658 1106 3 0.14344923013617716 1106 4 0.11033134679446022 1106 5 0.1533109598253688 1106 6 0.13362730919924104 1106 7 0.11666522517203504 1106 8 0.11304110368183728 1106 9 0.19185530192136468 1106 10-11 0.1877833882053821 1106 12-13 0.11858540946376905 1106 14-15 0.2112207651174529 1106 16-17 0.21583259498374474 1106 18-19 0.177774959133302 1106 20-21 0.17247568390861545 1106 22-23 0.2053767609252901 1106 24-25 0.16823850606820656 1106 26-27 0.21953657140251437 1106 28-29 0.24367368124463695 1106 30-31 0.18557017990628566 1106 32-33 0.20842497622171408 1106 34-35 0.20874165345080797 1106 36-37 0.2117646951682488 1106 38-39 0.20580798943325362 1106 40-41 0.2801330479907165 1106 42-43 0.25757794364244546 1106 44-45 0.3157402076833691 1106 46-47 0.3595496186358531 1106 48-49 0.31556079008129956 1106 50-51 0.2254948199114395 1106 52-53 0.23469000756021074 1106 54-55 0.25729589523572116 1106 56-57 0.27862923176559207 1106 58-59 0.21547828010459114 1106 60-61 0.19744799443802208 1106 62-63 0.13725231762533951 1106 64-65 0.29822562604609715 1106 66-67 0.25594095391292626 1106 68-69 0.24082955497098624 1106 70-71 0.3017734079953911 1106 72-73 0.26323937892315996 1106 74-75 0.2142076253783145 1106 76-77 0.08586463499089092 1106 78-79 0.20793571070259986 1106 80-81 0.2341422371504862 1106 82-83 0.23005883857150877 1106 84-85 0.23995009969768688 1106 86-87 0.27476565856029467 1106 88-89 0.26401645621125525 1106 90-91 0.28905491487570956 1106 92-93 0.29341968625443116 1106 94-95 0.32103792782595164 1106 96-97 0.36738595927263873 1106 98-99 0.3788967724844312 1106 100 0.397914147882549 1107 1 0.15539376480049327 1107 2 0.10769263642607285 1107 3 0.14363956278550916 1107 4 0.11727079982995292 1107 5 0.16800802132959802 1107 6 0.1339272180554687 1107 7 0.07986483004593481 1107 8 0.10492940927317562 1107 9 0.1185239713749624 1107 10-11 0.18790467562397595 1107 12-13 0.17473298091488942 1107 14-15 0.22196615828320176 1107 16-17 0.24155932658823076 1107 18-19 0.2398357250465466 1107 20-21 0.2546724017142452 1107 22-23 0.21761446797187034 1107 24-25 0.2297642223615597 1107 26-27 0.2226314373091398 1107 28-29 0.24195116567636177 1107 30-31 0.3242756327482894 1107 32-33 0.3270494689480259 1107 34-35 0.3501730181124785 1107 36-37 0.3536812604226114 1107 38-39 0.30826026624556135 1107 40-41 0.3103517421396873 1107 42-43 0.3920427667267745 1107 44-45 0.35333757622502304 1107 46-47 0.34125517840631403 1107 48-49 0.31440911970607743 1107 50-51 0.26909041649584253 1107 52-53 0.32381461994456373 1107 54-55 0.3124303959562198 1107 56-57 0.427950083323438 1107 58-59 0.44306475212752616 1107 60-61 0.3788569041242127 1107 62-63 0.38362245301712505 1107 64-65 0.30358151958608914 1107 66-67 0.3043084619198311 1107 68-69 0.3318316273462756 1107 70-71 0.3623996523116375 1107 72-73 0.3316369796523091 1107 74-75 0.3236655999497131 1107 76-77 0.24190023707446784 1107 78-79 0.3244142511801087 1107 80-81 0.3119145100930041 1107 82-83 0.3078017825664574 1107 84-85 0.3427931745526962 1107 86-87 0.33932620807071245 1107 88-89 0.3668863650165548 1107 90-91 0.4008055882703836 1107 92-93 0.37097331596628536 1107 94-95 0.34769921412434357 1107 96-97 0.42286456594395716 1107 98-99 0.406286139722571 1107 100 0.45111146143241854 1108 1 0.11500078780520795 1108 2 0.07394116519412819 1108 3 0.10262463160512425 1108 4 0.11212046667495201 1108 5 0.12957487119098943 1108 6 0.11334397794068707 1108 7 0.11205723313916849 1108 8 0.0813826946192222 1108 9 0.08878845267451396 1108 10-11 0.13269413138974073 1108 12-13 0.12259213173440742 1108 14-15 0.12475269590296278 1108 16-17 0.1429221007723669 1108 18-19 0.2088876194096656 1108 20-21 0.2177185570568838 1108 22-23 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sequence quality scores pass #Quality Count 2 20.0 3 25.0 4 39.0 5 110.0 6 236.0 7 624.0 8 1505.0 9 2632.0 10 4626.0 11 8453.0 12 13730.0 13 19393.0 14 22820.0 15 30895.0 16 41463.0 17 55724.0 18 57078.0 19 36226.0 20 24451.0 21 21333.0 22 22040.0 23 24428.0 24 27669.0 25 32327.0 26 39179.0 27 49338.0 28 62925.0 29 79357.0 30 100916.0 31 126785.0 32 162149.0 33 212196.0 34 302592.0 35 492248.0 36 965809.0 37 1933943.0 38 1761940.0 39 304288.0 40 235.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.27426887106282 18.288643428967273 13.322972268103356 24.114115431866555 2 15.182712573066839 22.56185245897912 35.631694241275746 26.623740726678292 3 20.08508710063929 27.06255606479972 29.28247709999297 23.56987973456802 4 14.300052249267777 18.783476847211862 33.56301802791466 33.3534528756057 5 13.828329816450378 37.15191698878134 33.0109701470388 16.00878304772949 6 31.84198466658913 33.133496559873564 17.927454650103165 17.09706412343414 7 25.380732934597056 32.425334224589434 22.228148781829283 19.965784058984227 8 28.085416871693912 31.02012895379513 21.37657033119764 19.517883843313314 9 28.27491530155798 14.541931142939388 19.628765418581498 37.55438813692113 10-11 25.306209915790674 25.869643261365304 25.478333615934023 23.345813206909998 12-13 27.68143198769224 23.855308985219768 25.85891278379985 22.60434624328814 14-15 26.386775490566 23.250344056907743 24.60087481554559 25.76200563698066 16-17 23.539930585141132 26.336718869821578 27.385297910169676 22.738052634867618 18-19 25.034228011883986 25.278237062478954 28.455239871583004 21.232295054054056 20-21 24.582795483459904 25.150675480741754 27.700912889729175 22.56561614606917 22-23 23.385054873734138 25.88953818438611 25.345952522885213 25.37945441899454 24-25 23.638245414297536 26.899746389240146 24.74286558643929 24.719142610023027 26-27 24.645104311917105 25.026481467517087 25.476236832579325 24.852177387986483 28-29 23.917667073262695 24.313128933262725 27.94174642013001 23.82745757334457 30-31 25.55355048413078 24.77173831077048 26.718230785693397 22.956480419405338 32-33 22.423792332593003 25.77662934333627 27.42080049692803 24.37877782714269 34-35 22.140926433528218 26.516967606331963 27.78457741049899 23.55752854964083 36-37 24.512138203029153 26.000329618837213 26.145065094039715 23.34246708409392 38-39 25.106931131210512 24.68014391358141 25.963409798666408 24.249515156541666 40-41 23.677211418031746 25.87568333239487 26.21492024673351 24.232185002839874 42-43 25.870511464095262 24.947703275557227 25.965838487264875 23.215946773082628 44-45 25.732004586944253 24.514731830623887 26.19809776723743 23.55516581519443 46-47 24.02864521258002 25.21290096825357 28.09358333633336 22.664870482833045 48-49 25.049968093839258 25.789053520623124 27.044154618809955 22.11682376672766 50-51 23.119671677637633 26.62439275302715 26.05977803958637 24.196157529748852 52-53 22.72863740896705 26.94095339916711 24.236760447584093 26.09364874428175 54-55 23.731903677166695 26.54269378996772 23.890231744141843 25.83517078872375 56-57 24.598864129892462 24.715192626680977 24.55792649905486 26.128016744371696 58-59 23.997356184467925 24.58162492806107 26.522013933441958 24.899004954029046 60-61 24.56796565500875 25.348941051453494 25.544879149843997 24.53821414369376 62-63 22.065172214497423 25.94287700966976 26.358049163807944 25.633901612024868 64-65 21.7333026664365 26.20081103479971 26.35202705087299 25.713859247890802 66-67 24.074957957798464 25.794506012297543 25.028004465945465 25.102531563958536 68-69 24.54395605800121 24.79236388764786 24.887860025908715 25.775820028442215 70-71 23.113796086646815 25.739554848406698 25.171562807807618 25.975086257138862 72-73 24.710181536612115 25.07608078597581 25.107280671514697 25.106457005897376 74-75 24.994070776326392 24.534523987721748 25.231339135404152 25.240066100547704 76-77 23.344485751713922 25.593788349825747 26.676088198661358 24.385637699798977 78-79 23.749359082187944 25.785931569113785 26.167610064076513 24.297099284621755 80-81 22.332774807160778 26.108279664123124 25.902393255537298 25.6565522731788 82-83 22.180986662656245 26.5159109567535 24.28018717753506 27.02291520305519 84-85 22.81336577414667 26.34338467428608 23.851929073850567 26.99132047771668 86-87 23.265870978690533 24.925554251420643 24.580112344950546 27.228462424938275 88-89 23.100672660163827 24.389813715155466 25.80553564650116 26.703977978179548 90-91 23.783959109897264 25.126148443615353 25.03406845842995 26.055823988057437 92-93 22.000711193313645 25.667908225388068 25.559134559535885 26.772246021762403 94-95 21.238788123221294 26.07678750972785 26.07312359052721 26.61130077652365 96-97 23.067825670557255 25.56799022513836 24.871294175804508 26.49288992849988 98-99 23.2930407752508 25.02531687094126 24.69580347036041 26.98583888344753 100 22.640980241123728 25.87565552389652 25.347941745852136 26.13542248912762 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 842.0 1 563.0 2 311.5 3 361.5 4 349.0 5 388.0 6 622.5 7 1270.0 8 1962.0 9 2047.5 10 1648.5 11 1449.0 12 1597.5 13 1836.0 14 2210.0 15 2526.0 16 2924.0 17 3417.0 18 3965.0 19 4664.0 20 5476.5 21 6385.5 22 7674.5 23 9343.0 24 11227.0 25 13766.5 26 17287.5 27 21638.5 28 26587.5 29 33632.0 30 41670.0 31 48981.0 32 57054.0 33 65785.5 34 74462.5 35 81160.0 36 87694.5 37 97152.0 38 104038.0 39 108995.0 40 112438.0 41 115569.0 42 122616.5 43 138011.0 44 158271.5 45 177046.0 46 203444.0 47 242169.5 48 336407.0 49 482879.0 50 713845.5 51 687924.0 52 410805.5 53 296741.0 54 260751.5 55 231702.5 56 209470.0 57 186911.0 58 174799.0 59 160410.0 60 134665.5 61 111080.5 62 90432.5 63 71314.5 64 53884.5 65 42259.0 66 32718.0 67 24281.0 68 18885.0 69 15574.0 70 13019.5 71 10986.5 72 9528.5 73 9471.5 74 7421.0 75 5714.5 76 4485.5 77 2913.5 78 2069.5 79 1321.0 80 894.0 81 695.0 82 507.0 83 348.0 84 221.5 85 125.5 86 72.5 87 42.0 88 22.5 89 13.5 90 9.0 91 4.5 92 3.5 93 4.0 94 1.5 95 0.0 96 0.5 97 0.5 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.06184544829571411 3 0.027010342745912342 4 0.007100510711333424 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2780919280400163E-4 12-13 1.491107249380019E-4 14-15 4.8283472837067277E-4 16-17 3.8342757841200486E-4 18-19 0.0 20-21 0.0029325109237807036 22-23 0.005034262094335397 24-25 7.171515818446758E-4 26-27 4.260306426800054E-4 28-29 0.005112367712160065 30-31 0.002243761384781362 32-33 0.0011857852887926818 34-35 0.0015408108243593528 36-37 0.00398338650905805 38-39 0.004246105405377387 40-41 8.591617960713443E-4 42-43 0.0027976012202653687 44-45 3.1242247129867063E-4 46-47 0.002151454745534027 48-49 4.970357497933397E-5 50-51 0.001860333806369357 52-53 0.006788088240034753 54-55 0.014009307633460843 56-57 0.025128707407408985 58-59 0.027287262663654346 60-61 0.025114506385986318 62-63 0.008115883743054103 64-65 0.007036606114931422 66-67 1.84613278494669E-4 68-69 0.0014343031636893516 70-71 1.5621123564933532E-4 72-73 7.739556675353432E-4 74-75 0.005012960562201397 76-77 0.0010721771174113468 78-79 0.0012425893744833492 80-81 0.0 82-83 0.0012780919280400162 84-85 0.0 86-87 5.964428997520076E-4 88-89 5.680408569066739E-5 90-91 6.106439211746744E-4 92-93 8.662623067826776E-4 94-95 0.0015195092922253527 96-97 1.491107249380019E-4 98-99 0.0 100 7.242520925560092E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7041747.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 32.79620676076268 #Duplication Level Percentage of deduplicated Percentage of total 1 76.19951048649793 24.9905490098409 2 12.461222053088964 8.173616298897626 3 4.0347432024202075 3.9697281687946466 4 1.9083566002446946 2.5034743053956454 5 1.1096075877149245 1.8195459935004887 6 0.7667541581529927 1.508797674327605 7 0.5201588142497222 1.1941465214396927 8 0.3840623281076782 1.0076630017311432 9 0.2844072726340226 0.8394731745813008 >10 1.943761150669652 12.274879690637308 >50 0.19641236378248444 4.491276865879094 >100 0.15929602209289406 10.760456521810461 >500 0.019948146448848618 4.3975383936846875 >1k 0.009364296249860762 5.279322129914902 >5k 7.839875930100476E-4 1.7806434698854339 >10k+ 0.0016115300522984313 15.0088887796789 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 131506 1.8675195232092263 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 122720 1.7427493489896755 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 61548 0.8740444665222992 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 49444 0.7021553032223395 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 46017 0.6534884028068603 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 45165 0.641389132554748 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 44973 0.6386625364415961 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 40438 0.5742609042898019 No Hit GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT 36786 0.5223987740542226 No Hit ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC 33331 0.4733342450389087 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 32365 0.4596160583446125 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 29519 0.41919995137570265 No Hit ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG 28117 0.39929011934112374 No Hit GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC 21157 0.30045101023936244 No Hit CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC 20631 0.2929812729710397 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19087 0.2710548958944421 No Hit CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT 18561 0.26358515862611936 No Hit ATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTA 18091 0.25691067855746597 No Hit GTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCAC 17796 0.2527213772377792 No Hit GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG 17463 0.24799243710403115 No Hit GATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGT 16536 0.23482809024521897 No Hit CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA 15135 0.21493245923206272 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 14621 0.20763313422081198 No Hit GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA 14590 0.20719290255670927 No Hit GTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCA 13552 0.1924522423199811 No Hit GCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAG 13252 0.18819193589318106 No Hit TACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTAC 13128 0.18643100923677036 No Hit CCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTC 12544 0.17813761272593293 No Hit GCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAG 12213 0.1734370746350302 No Hit GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG 12191 0.17312465216373152 No Hit ACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATC 12064 0.17132112244305284 No Hit CCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATA 11714 0.16635076494511944 No Hit CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC 11148 0.15831298681989 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11078 0.15731891532030334 No Hit ATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGG 10919 0.1550609529140993 No Hit ATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAA 10748 0.15263257825082327 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC 10575 0.1501758015447019 No Hit CTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTT 9647 0.13699725366446708 No Hit AAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAG 8825 0.12532401405503493 No Hit GGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGC 8785 0.12475597319812826 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 8633 0.12259741794188289 No Hit GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG 8241 0.11703061754419748 No Hit TGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATG 8187 0.11626376238737347 No Hit GCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACCACTGCTTCCA 7962 0.11306853256727344 No Hit TTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTG 7769 0.11032773543269873 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0029254104130693706 0.0 0.0 1.4201021422666846E-5 0.0 2 0.00499875954077873 0.0 0.0 1.4201021422666846E-5 0.0 3 0.0052685789478094 0.0 0.0 1.4201021422666846E-5 0.0 4 0.0065466708758494165 0.0 0.0 2.8402042845333692E-5 0.0 5 0.02509320485385232 0.0 0.0 1.5621123564933532E-4 0.0 6 0.05404908753467002 0.0 0.0 2.698194070306701E-4 0.0 7 0.06095078394608611 0.0 0.0 2.982214498760038E-4 0.0 8 0.06985482437809822 0.0 0.0 3.8342757841200486E-4 0.0 9 0.0764582993396383 0.0 0.0 4.8283472837067277E-4 0.0 10-11 0.13564815662931373 0.0 0.0 9.72769967452679E-4 0.0 12-13 0.2395712314003897 0.0 0.0 0.0026768925381727006 0.0 14-15 0.36103966813917054 0.0 0.0 0.004842548305129394 0.0 16-17 0.41605442513058194 0.0 0.0 0.0057372126547574065 0.0 18-19 0.4432422806442776 1.4201021422666846E-5 0.0 0.0062910524902414135 0.0 20-21 0.5411867253964109 1.4201021422666846E-5 7.100510711333423E-6 0.008293396510837438 0.0 22-23 0.5686799028706939 1.4201021422666846E-5 1.4201021422666846E-5 0.010246036956454131 0.0 24-25 0.6901625406308974 1.4201021422666846E-5 1.4201021422666846E-5 0.020002138673826254 0.0 26-27 0.9547417707566035 1.4201021422666846E-5 1.4201021422666846E-5 0.051855029724867996 0.0 28-29 1.0184617538801097 1.4201021422666846E-5 1.4201021422666846E-5 0.08137185275188102 0.0 30-31 1.0365183526190305 1.4201021422666846E-5 1.4201021422666846E-5 0.14984917805198056 0.0 32-33 1.0528566277658087 1.4201021422666846E-5 1.4201021422666846E-5 0.26563010571098333 0.0 34-35 1.0658789644103943 2.8402042845333692E-5 1.4201021422666846E-5 0.4272306289902208 0.0 36-37 1.0855686806129219 2.8402042845333692E-5 1.4201021422666846E-5 0.6669580716262598 0.0 38-39 1.105932945333026 2.8402042845333692E-5 1.4201021422666846E-5 1.0053258090641428 0.0 40-41 1.1239043379434108 2.8402042845333692E-5 1.4201021422666846E-5 1.461086290092501 0.0 42-43 1.1599962338891188 2.8402042845333692E-5 1.4201021422666846E-5 1.8020812164935776 0.0 44-45 1.2031815400354486 2.8402042845333692E-5 1.4201021422666846E-5 2.169802465212113 0.0 46-47 1.2216570689063382 2.8402042845333692E-5 1.4201021422666846E-5 2.598169175916147 1.4201021422666846E-5 48-49 1.2333516100479045 2.8402042845333692E-5 1.4201021422666846E-5 2.9788985602578455 2.8402042845333692E-5 50-51 1.2544685289034099 2.8402042845333692E-5 1.4201021422666846E-5 3.3847850540497975 2.8402042845333692E-5 52-53 1.2609938982471252 2.8402042845333692E-5 1.4201021422666846E-5 3.849399871935189 2.8402042845333692E-5 54-55 1.2822457268061462 4.260306426800054E-5 1.4201021422666846E-5 4.287529784867306 2.8402042845333692E-5 56-57 1.3223991148787368 9.940714995866793E-5 1.4201021422666846E-5 4.754615580480241 2.8402042845333692E-5 58-59 1.3364013220014863 9.940714995866793E-5 1.4201021422666846E-5 5.237940244089996 2.8402042845333692E-5 60-61 1.3462710318902396 9.940714995866793E-5 1.4201021422666846E-5 5.669821707596141 2.8402042845333692E-5 62-63 1.353769171201408 9.940714995866793E-5 1.4201021422666846E-5 6.111572880991038 2.8402042845333692E-5 64-65 1.359989218584536 9.940714995866793E-5 1.4201021422666846E-5 6.603815786054228 2.8402042845333692E-5 66-67 1.368296816116796 9.940714995866793E-5 1.4201021422666846E-5 7.11209874481432 2.8402042845333692E-5 68-69 1.3781665260055496 9.940714995866793E-5 1.4201021422666846E-5 7.706901426592009 2.8402042845333692E-5 70-71 1.3904149069825995 1.1360817138133477E-4 1.4201021422666846E-5 8.422838821105046 2.8402042845333692E-5 72-73 1.4182063059067587 1.3490970351533505E-4 1.4201021422666846E-5 9.011158736603289 2.8402042845333692E-5 74-75 1.445791790020289 1.4201021422666847E-4 1.4201021422666846E-5 9.576607907100325 2.8402042845333692E-5 76-77 1.4550011524128883 1.4201021422666847E-4 1.4201021422666846E-5 10.203945128957345 2.8402042845333692E-5 78-79 1.4612425013281505 1.4201021422666847E-4 1.4201021422666846E-5 10.821540450118416 2.8402042845333692E-5 80-81 1.4738104052872107 1.4201021422666847E-4 1.4201021422666846E-5 11.490145840229705 2.8402042845333692E-5 82-83 1.4790079791279067 1.4201021422666847E-4 1.4201021422666846E-5 12.254608124943996 2.8402042845333692E-5 84-85 1.4968586630561989 1.4201021422666847E-4 1.4201021422666846E-5 12.978860217500003 2.8402042845333692E-5 86-87 1.5269506274508302 1.4201021422666847E-4 1.4201021422666846E-5 13.700087492492985 2.8402042845333692E-5 88 1.534491369826266 1.4201021422666847E-4 1.4201021422666846E-5 14.288244096244867 2.8402042845333692E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCCGATC 1965 0.0 26.078724 3 CGATCTC 2020 0.0 20.937082 5 AGTACTT 22920 0.0 19.467562 12-13 GATCTAC 2655 0.0 18.958097 1 GAGTACT 21940 0.0 18.484402 12-13 TCTACAC 3655 0.0 18.0079 3 CCGATCT 3015 0.0 17.146303 4 CGTTAAC 665 0.0 16.977161 1 TACTTTT 26810 0.0 16.748856 14-15 TAGTACT 1925 0.0 16.601345 4 TACACTC 3570 0.0 16.585453 5 GTACTAG 1770 0.0 16.477598 1 AGTACTC 2235 0.0 16.189678 5 GTATAAG 1835 0.0 16.150278 1 GTACTTT 25270 0.0 16.123714 14-15 CCGTCGT 2300 0.0 15.732478 9 CATGGGG 16115 0.0 15.660625 4 GTAATAC 875 0.0 15.581611 3 ATCTACA 3615 0.0 15.485105 2 GTATTAG 2170 0.0 14.957702 1 >>END_MODULE