##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765475_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 16955828 Sequences flagged as poor quality 0 Sequence length 100 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.886935512674462 33.0 33.0 33.0 27.0 33.0 2 31.75971506670155 33.0 33.0 33.0 27.0 33.0 3 31.818354373493293 33.0 33.0 33.0 27.0 33.0 4 35.550365160580775 37.0 37.0 37.0 33.0 37.0 5 35.626552593008135 37.0 37.0 37.0 33.0 37.0 6 35.600626404089496 37.0 37.0 37.0 33.0 37.0 7 35.53765714065984 37.0 37.0 37.0 33.0 37.0 8 35.51925096197012 37.0 37.0 37.0 33.0 37.0 9 35.465973233510034 37.0 37.0 37.0 33.0 37.0 10-11 35.46464625614273 37.0 37.0 37.0 33.0 37.0 12-13 35.41606169277018 37.0 37.0 37.0 33.0 37.0 14-15 36.99797131700086 40.0 37.0 40.0 33.0 40.0 16-17 37.05170183962706 40.0 37.0 40.0 33.0 40.0 18-19 37.038752103406566 40.0 37.0 40.0 33.0 40.0 20-21 37.1434592577844 40.0 37.0 40.0 33.0 40.0 22-23 37.15206329056888 40.0 37.0 40.0 33.0 40.0 24-25 37.14101865152206 40.0 37.0 40.0 33.0 40.0 26-27 37.098213222026075 40.0 37.0 40.0 33.0 40.0 28-29 37.05681282565499 40.0 37.0 40.0 33.0 40.0 30-31 36.96837055082182 40.0 37.0 40.0 33.0 40.0 32-33 36.83891603524169 37.0 37.0 40.0 33.0 40.0 34-35 36.7256387597232 37.0 37.0 40.0 33.0 40.0 36-37 36.633755249227576 37.0 37.0 40.0 33.0 40.0 38-39 36.508776038539665 37.0 37.0 40.0 33.0 40.0 40-41 36.37502102521917 37.0 37.0 40.0 33.0 40.0 42-43 36.21214033310552 37.0 37.0 40.0 33.0 40.0 44-45 36.00794847647664 37.0 37.0 40.0 27.0 40.0 46-47 35.787237963253695 37.0 37.0 40.0 27.0 40.0 48-49 35.50522938189748 37.0 35.0 40.0 27.0 40.0 50-51 35.37223572921357 37.0 33.0 40.0 27.0 40.0 52-53 35.194414480967836 37.0 33.0 40.0 27.0 40.0 54-55 35.022983601862435 37.0 33.0 40.0 27.0 40.0 56-57 34.82803331692206 37.0 33.0 40.0 27.0 40.0 58-59 34.62658562000039 37.0 33.0 38.5 27.0 40.0 60-61 34.41935822892282 37.0 33.0 37.0 27.0 40.0 62-63 34.180048771431274 37.0 33.0 37.0 27.0 40.0 64-65 33.98693794251746 37.0 33.0 37.0 27.0 40.0 66-67 33.80438834953976 37.0 33.0 37.0 27.0 40.0 68-69 33.61208724221548 37.0 33.0 37.0 27.0 40.0 70-71 33.44945876426678 37.0 33.0 37.0 27.0 38.5 72-73 33.25567212052398 37.0 33.0 37.0 27.0 37.0 74-75 33.042999698982555 37.0 33.0 37.0 27.0 37.0 76-77 32.86664278500584 37.0 33.0 37.0 22.0 37.0 78-79 32.723112135839074 37.0 33.0 37.0 22.0 37.0 80-81 32.59995185726112 37.0 33.0 37.0 22.0 37.0 82-83 32.48042127462015 37.0 33.0 37.0 22.0 37.0 84-85 32.354764863149114 33.0 33.0 37.0 22.0 37.0 86-87 32.249788302877334 33.0 33.0 37.0 22.0 37.0 88-89 32.15257594615845 33.0 33.0 37.0 22.0 37.0 90-91 32.03296524357289 33.0 33.0 37.0 22.0 37.0 92-93 31.923283663882412 33.0 33.0 37.0 22.0 37.0 94-95 31.834053105516286 33.0 33.0 37.0 22.0 37.0 96-97 31.75961185735076 33.0 33.0 37.0 22.0 37.0 98-99 31.663976657465504 33.0 33.0 37.0 22.0 37.0 100 31.58641229434505 33.0 33.0 37.0 22.0 37.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 2.0 5 4.0 6 9.0 7 179.0 8 1130.0 9 2228.0 10 3458.0 11 6161.0 12 12529.0 13 24339.0 14 39462.0 15 46946.0 16 52325.0 17 63162.0 18 76277.0 19 91126.0 20 107124.0 21 126539.0 22 148995.0 23 147096.0 24 140389.0 25 147672.0 26 164729.0 27 193429.0 28 237275.0 29 299612.0 30 383444.0 31 495673.0 32 654919.0 33 864005.0 34 1137894.0 35 1577583.0 36 2707536.0 37 4848164.0 38 2152149.0 39 2264.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53592481305636 20.509776623228312 12.832642701631592 24.12165586208373 2 15.729430505173278 21.64550768348061 37.9237262144278 24.7013355969183 3 17.867311520370396 28.321019183414677 29.43346259897669 24.378206697238237 4 12.555890019176447 18.015025973782397 37.95961633894173 31.469467668099426 5 13.085603087585637 38.86560376023654 33.47452511731563 14.574268034862198 6 29.313360574311087 38.64557956119866 18.15550971618726 13.885550148302992 7 25.43906437361832 32.89409989296895 23.47084436100673 18.195991372405995 8 26.751775259810373 32.7766240610603 20.42087239856408 20.05072828056524 9 27.100038995441565 14.473289066154718 20.850724600414676 37.57594733798904 10-11 25.290186359521933 26.247343981078362 26.372253835082542 22.090215824317163 12-13 26.11997479568677 24.466009563201514 26.551386933153605 22.862628707958113 14-15 24.386615032896064 24.298031921531642 24.804064419620204 26.51128862595209 16-17 23.11019857968055 26.61818580502688 26.04896341143716 24.222652203855407 18-19 23.303812260971952 26.41407747231277 28.192811359819757 22.089298906895515 20-21 25.02324657785869 25.383664741131778 26.66200609629027 22.931082584719263 22-23 24.702849675833193 25.136915204331295 26.167361897161644 23.992873222673865 24-25 23.73093463580495 26.03954711938567 26.61155928521659 23.617958959592787 26-27 24.10716155177247 25.624572725024507 26.30362107413515 23.964644649067868 28-29 23.277978228611087 25.748190559675177 27.16205960748735 23.811771604226387 30-31 24.16017747159663 25.548699113718566 26.825392579133517 23.46573083555129 32-33 23.54832635227448 26.006699162199208 26.112974447186403 24.332000038339913 34-35 23.81338044026404 25.861260211063975 27.195729161416395 23.129630187255596 36-37 24.149644616155953 25.60321762467927 26.45027870351255 23.796859055652224 38-39 24.406144306959217 24.972901082632387 26.623237021903677 23.99771758850472 40-41 23.696190557968322 25.24050872399873 27.21476233009941 23.848538387933537 42-43 24.47844493086791 25.0831132905525 26.49807429632124 23.940367482258353 44-45 23.993186907669394 25.659274717294007 26.42345154364893 23.924086831387672 46-47 23.611914569411926 25.350499036823333 26.74313092554798 24.294455468216764 48-49 24.597830793876874 25.46222897125935 26.257001528796703 23.682938706067073 50-51 23.650923333439405 26.007299184181935 25.574996329056127 24.766781153322533 52-53 23.239150068480306 26.047584350027336 25.275908533923314 25.437357047569044 54-55 23.55060244368315 26.010328381316082 25.336410378689422 25.102658796311346 56-57 24.349675775009356 25.059761978422753 25.136973150979696 25.453589095588196 58-59 23.521194964582083 25.206538531970445 26.30093903632743 24.971327467120044 60-61 23.742207456804177 25.636971252963114 25.69370810783135 24.927113182401364 62-63 23.474764361091687 25.33699969591174 25.358872814715866 25.829363128280708 64-65 22.83267588008574 25.55791118761517 25.835893924420656 25.773519007878438 66-67 24.219843893718856 25.08002725986373 25.51441608465269 25.185712761764723 68-69 23.854742334516942 24.851038743860958 25.649918450817683 25.64430047080442 70-71 23.2128758322517 25.178689118858657 25.75630344291297 25.852131605976673 72-73 24.293480415033034 24.99572077991632 25.75812975137938 24.95266905367127 74-75 24.32493555301232 25.24744231339568 25.817662171127353 24.609959962464643 76-77 23.643277161661914 25.422484856981736 25.866136644561834 25.06810133679452 78-79 23.591637911382904 25.718370932691553 25.699833469528343 24.9901576863972 80-81 23.148044253613147 25.56886501069733 26.393021010731886 24.89006972495764 82-83 23.719076726384493 25.211819886732545 26.109928657437926 24.95917472944504 84-85 23.92431686786784 25.612436267634635 25.180593895983378 25.282652968514146 86-87 23.255096613312283 25.312485227790095 25.887505524419456 25.544912634478163 88-89 23.65113825741815 24.451383674702136 25.94496299779465 25.95251507008506 90-91 23.708457008645123 24.7502961837939 25.914120972266062 25.62712583529492 92-93 23.67882432831407 24.875964414862377 25.916631289994857 25.528579966828698 94-95 22.614267842440807 25.092808326723524 26.439890368998302 25.85303346183736 96-97 23.337676736231003 24.966342239919033 25.708489399033045 25.987491624816922 98-99 23.11355311031257 25.264444326685258 25.65865254452882 25.963350018473353 100 23.274605886191722 25.059560512861793 25.39157185199209 26.27426174895439 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1749.0 1 1350.5 2 1271.5 3 1543.0 4 1252.5 5 1065.5 6 1386.5 7 2751.0 8 4196.0 9 3820.5 10 2419.5 11 1725.5 12 1812.0 13 2098.5 14 2506.5 15 3249.5 16 4034.0 17 4963.5 18 6315.0 19 7691.0 20 9398.5 21 11645.5 22 14080.5 23 16947.0 24 20675.5 25 25244.5 26 31791.5 27 39007.5 28 49747.5 29 72296.5 30 100546.5 31 126598.0 32 161375.0 33 195920.0 34 232481.0 35 261001.5 36 284850.5 37 340995.0 38 365059.0 39 357560.0 40 348061.0 41 336622.0 42 346359.5 43 387064.0 44 460094.0 45 552116.0 46 654758.5 47 750403.5 48 844662.5 49 892842.0 50 958273.5 51 953749.0 52 827301.5 53 768457.0 54 734276.0 55 669834.0 56 627599.0 57 554891.5 58 474002.5 59 434697.0 60 367541.0 61 272329.0 62 202259.5 63 157813.5 64 114137.0 65 86755.0 66 74452.5 67 57298.5 68 42707.0 69 34066.0 70 28539.0 71 25223.0 72 23134.0 73 24821.5 74 19368.5 75 13572.0 76 11201.0 77 10103.0 78 8586.0 79 5685.0 80 4397.0 81 3999.5 82 4194.5 83 4480.5 84 3545.5 85 2234.0 86 1594.0 87 795.5 88 345.5 89 264.5 90 225.5 91 176.0 92 121.5 93 70.0 94 51.0 95 41.0 96 26.0 97 17.5 98 13.0 99 9.5 100 13.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017639952469440005 2 0.005968449314300664 3 0.006357696008711577 4 0.0038570808809808635 5 0.04693371506245522 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 3.833490172228687E-4 18-19 0.001843024121263792 20-21 6.281026205267003E-4 22-23 0.005304960630645699 24-25 0.007628645442735087 26-27 0.005879984156480002 28-29 0.02219295925861008 30-31 0.020532763130175654 32-33 0.01306630381011178 34-35 0.013815308812993385 36-37 0.013729792493766746 38-39 0.02112842852616811 40-41 0.030334702616705005 42-43 0.01807048290416723 44-45 0.016985310301567108 46-47 0.018111766644483537 48-49 0.013555811016719444 50-51 0.01027080482297886 52-53 0.027149956935161174 54-55 0.02527154675076912 56-57 0.013700304107826524 58-59 0.016256947168843655 60-61 0.017707775757102515 62-63 0.020718539961599045 64-65 0.011190842464313745 66-67 0.015914881891937096 68-69 0.007876347884632942 70-71 0.008976264680203173 72-73 0.01063940964723162 74-75 0.0036182249548650765 76-77 0.007755445502278037 78-79 0.005738439903966943 80-81 0.0024858709347606025 82-83 1.828279928293682E-4 84-85 0.002662801250401927 86-87 0.002515359320700823 88-89 0.0012797959498055773 90-91 8.256748063261789E-4 92-93 0.0012385122094892682 94-95 0.003464885347975929 96-97 0.001347619237468085 98-99 0.0018961032159561892 100 0.0022588103630209037 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.6955828E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 16.09772690889725 #Duplication Level Percentage of deduplicated Percentage of total 1 66.35151492526603 10.681085672585525 2 14.14244480902807 4.5532242871977076 3 6.292556313616291 3.038875592863568 4 3.4958203848640927 2.25099047512393 5 2.0482785402242545 1.6486314286942378 6 1.386065710667203 1.3387504372864334 7 0.9722873594089554 1.0956131472116735 8 0.7344053204257514 0.945780503092394 9 0.5368681442632756 0.7778121095192995 >10 3.2144601557949466 9.701216307247032 >50 0.33974551879732184 3.849074599514394 >100 0.3342749961571023 11.592114621834309 >500 0.06531434392599425 7.445493781696282 >1k 0.07531967677257766 25.27567132693258 >5k 0.008130681157830118 8.686720896789776 >10k+ 0.002513119630602036 7.118944812410849 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 75894 0.44759831250942156 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 73509 0.4335323524159363 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 31869 0.1879530743057785 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 30353 0.1790121956887036 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 27895 0.16451570516049113 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 27418 0.16170251314179407 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 26720 0.15758593446453928 No Hit CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC 24184 0.14262942511565935 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 23744 0.14003444715291993 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 23556 0.13892568384156762 No Hit ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC 23096 0.13621275233506733 No Hit TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA 22743 0.13413087228768775 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 22702 0.13388906752297794 No Hit GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT 22487 0.13262106692754846 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 22401 0.13211386668937664 No Hit GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC 22262 0.13129408956023852 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 21484 0.12670569670794019 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 19729 0.11635527324292273 No Hit GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC 19276 0.11368362547673873 No Hit ATACTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATA 19243 0.11348900212953328 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 18887 0.11138942905058956 No Hit CTATATAGGTGGCTATCTATCCCTACCAAGGCTTATATTGAAGTATAAAC 18209 0.10739080391709563 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 17537 0.10342756484672999 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.7693031564132403E-5 2 5.897677188044135E-6 0.0 0.0 0.0 1.7693031564132403E-5 3 5.897677188044135E-6 0.0 0.0 0.0 1.7693031564132403E-5 4 5.897677188044135E-6 0.0 0.0 5.897677188044135E-6 2.9488385940220672E-5 5 1.179535437608827E-5 0.0 0.0 5.897677188044135E-6 2.9488385940220672E-5 6 2.359070875217654E-5 0.0 0.0 5.897677188044135E-6 4.718141750435308E-5 7 2.9488385940220672E-5 0.0 0.0 2.9488385940220672E-5 4.718141750435308E-5 8 2.9488385940220672E-5 0.0 0.0 2.9488385940220672E-5 4.718141750435308E-5 9 2.9488385940220672E-5 0.0 0.0 4.128374031630894E-5 7.666980344457375E-5 10-11 4.128374031630894E-5 0.0 0.0 4.718141750435308E-5 8.846515782066202E-5 12-13 4.718141750435308E-5 0.0 0.0 7.666980344457375E-5 1.533396068891475E-4 14-15 5.307909469239721E-5 0.0 0.0 7.666980344457375E-5 2.152652173636109E-4 16-17 5.602793328641928E-5 0.0 0.0 1.356465753250151E-4 2.5654895767991985E-4 18-19 7.077212625652961E-5 0.0 0.0 2.152652173636109E-4 2.771908278380743E-4 20-21 8.846515782066202E-5 0.0 0.0 3.302699225304715E-4 3.007815365902509E-4 22-23 9.14139964146841E-5 0.0 0.0 7.725957116337817E-4 3.1257689096633913E-4 24-25 1.0615818938479441E-4 0.0 0.0 0.002155601012230131 3.243722453424274E-4 26-27 1.710326384532799E-4 0.0 0.0 0.0038128483020705324 3.332187611244936E-4 28-29 1.798791542353461E-4 0.0 0.0 0.013375931862484097 3.391164383125377E-4 30-31 1.887256700174123E-4 0.0 0.0 0.05295524347144828 3.4796295409460394E-4 32-33 1.887256700174123E-4 0.0 0.0 0.13180128979841033 3.656559856587363E-4 34-35 1.9462334720545644E-4 0.0 0.0 0.23193205309702364 3.921955330049349E-4 36-37 1.9462334720545644E-4 0.0 0.0 0.3913816535529848 3.95144371598957E-4 38-39 2.0052102439350057E-4 0.0 0.0 0.6715596548868035 4.0104204878700113E-4 40-41 2.0936754017556678E-4 0.0 0.0 1.040580265381319 4.0399088738102317E-4 42-43 2.1231637876958885E-4 0.0 0.0 1.3426533932757516 4.0988856456906735E-4 44-45 2.1821405595763297E-4 0.0 0.0 1.670505268159125 4.216839189451556E-4 46-47 2.1821405595763297E-4 0.0 0.0 2.0311069444677075 4.3642811191526594E-4 48-49 2.241117331456771E-4 0.0 0.0 2.4186344659783057 4.6591649785548663E-4 50-51 2.241117331456771E-4 0.0 0.0 2.838549081767048 4.7181417504353076E-4 52-53 2.241117331456771E-4 0.0 0.0 3.250242335555657 4.983537223897294E-4 54-55 2.241117331456771E-4 0.0 0.0 3.6330487664772253 5.2784210832995E-4 56-57 2.241117331456771E-4 0.0 0.0 4.063431759274746 5.366886241120162E-4 58-59 2.241117331456771E-4 0.0 0.0 4.552334453970635 5.425863013000604E-4 60-61 2.241117331456771E-4 0.0 0.0 5.059080570998951 5.602793328641927E-4 62-63 2.241117331456771E-4 0.0 0.0 5.571594616317174 6.074607503685458E-4 64-65 2.2706057173969916E-4 0.0 0.0 6.070013213155972 6.104095889625679E-4 66-67 2.3000941033372125E-4 0.0 0.0 6.589595624584066 6.310514591207224E-4 68-69 2.3000941033372125E-4 0.0 0.0 7.192049247019963 6.369491363087665E-4 70-71 2.3295824892774331E-4 0.0 0.0 7.890652700652542 6.398979749027887E-4 72-73 2.4770244189785363E-4 2.9488385940220674E-6 0.0 8.55499359866118 6.929770695951858E-4 74-75 2.5065128049187567E-4 5.897677188044135E-6 0.0 9.183597521748865 7.165677783473624E-4 76-77 2.6539547346198606E-4 5.897677188044135E-6 0.0 9.856578516837986 7.195166169413844E-4 78-79 2.712931506500302E-4 5.897677188044135E-6 0.0 10.542504913354865 7.313119713174726E-4 80-81 2.771908278380743E-4 5.897677188044135E-6 0.0 11.309524371207353 7.431073256935609E-4 82-83 2.771908278380743E-4 5.897677188044135E-6 0.0 12.13849008140446 7.87339904603892E-4 84-85 2.771908278380743E-4 5.897677188044135E-6 0.0 12.916296390833878 7.90288743197914E-4 86-87 2.771908278380743E-4 1.179535437608827E-5 0.0 13.707687999665955 8.286236449202009E-4 88 2.771908278380743E-4 1.179535437608827E-5 0.0 14.31991407320244 8.610608694544436E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACAAG 4350 0.0 28.0865 1 CTATTGA 12670 0.0 27.25516 9 AGGGCTA 12900 0.0 26.990522 5 GTACATA 2555 0.0 26.484095 1 TACATGA 4355 0.0 25.787954 2 GTATAAG 10260 0.0 24.91524 1 GTGATCG 8885 0.0 24.588585 8 GTACTAG 10915 0.0 24.582493 1 CGTTAAC 3540 0.0 24.557379 1 TATTCGC 1830 0.0 24.389927 9 ATTTAGG 14535 0.0 24.376417 1 TATTCTC 6095 0.0 24.36106 5 TTTAGGG 14845 0.0 24.05695 2 ACGTTAA 2345 0.0 23.441263 8 GGGCTAT 16045 0.0 22.956957 6 GGCTATT 15945 0.0 22.423227 7 ATAAGGT 10275 0.0 22.180151 3 GTACAAA 3710 0.0 21.785528 1 TGATCGC 10005 0.0 21.74212 9 TAGGCAT 16050 0.0 21.312735 5 >>END_MODULE