FastQCFastQC Report
Fri 27 May 2016
ERR765474_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765474_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11757674
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT178690.15197733837492006No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT177350.1508376571760707No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA165330.14061454672072043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA411850.021.1600571
GTACTAG50650.020.8809011
GTATAAG55250.020.3334851
TAGGCAT70900.018.956695
TAGTACT65900.018.6843094
GTACATG622400.018.4350531
CTAGTAC69400.017.8779853
CAACGCA448200.017.7931445
TACATGG621100.017.7300072
ATCAACG453050.017.5105763
AACGCAG471850.017.3590136
TATCAAC499600.017.2534142
ACATGGG603200.016.8293193
CTAGGCA82950.016.7701324
CATGGGG240350.016.6788484
ACGCAGA486550.016.350527
TCAACGC497100.016.0435054
GTATAAA151400.015.8960881
GTGATCG39850.015.7997048
TGATCGC39450.015.7207619
Produced by FastQC (version 0.11.5)