##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765474_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11757674
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.63000675133534	33.0	33.0	33.0	27.0	33.0
2	31.4963654375857	33.0	33.0	33.0	27.0	33.0
3	31.476667153724453	33.0	33.0	33.0	27.0	33.0
4	35.093263854738616	37.0	37.0	37.0	33.0	37.0
5	35.29484003383662	37.0	37.0	37.0	33.0	37.0
6	35.30605611279918	37.0	37.0	37.0	33.0	37.0
7	35.18174087834039	37.0	37.0	37.0	33.0	37.0
8	35.16656636338106	37.0	37.0	37.0	33.0	37.0
9	34.868435117354	37.0	37.0	37.0	33.0	37.0
10-11	34.86573071340471	37.0	37.0	37.0	33.0	37.0
12-13	34.84332704750957	37.0	37.0	37.0	33.0	37.0
14-15	36.109298743952245	37.0	37.0	40.0	30.0	40.0
16-17	36.35562437774682	37.0	37.0	40.0	33.0	40.0
18-19	36.17863703314108	37.0	37.0	40.0	27.0	40.0
20-21	36.17182114421611	37.0	37.0	40.0	27.0	40.0
22-23	36.34448837414611	37.0	37.0	40.0	33.0	40.0
24-25	36.357813203529886	37.0	37.0	40.0	33.0	40.0
26-27	36.03586240781978	37.0	37.0	40.0	27.0	40.0
28-29	35.923064204705796	37.0	37.0	40.0	27.0	40.0
30-31	35.73861003460378	37.0	37.0	40.0	27.0	40.0
32-33	35.71260267974771	37.0	37.0	40.0	27.0	40.0
34-35	35.67071050787766	37.0	37.0	40.0	27.0	40.0
36-37	35.44762620565939	37.0	33.0	40.0	27.0	40.0
38-39	35.331012494478074	37.0	33.0	40.0	27.0	40.0
40-41	35.488173851392716	37.0	33.0	40.0	27.0	40.0
42-43	35.42302036099997	37.0	33.0	40.0	27.0	40.0
44-45	35.38387358758203	37.0	33.0	40.0	27.0	40.0
46-47	35.36280079716447	37.0	33.0	40.0	27.0	40.0
48-49	35.203327843585384	37.0	33.0	40.0	27.0	40.0
50-51	35.06274374506386	37.0	33.0	40.0	27.0	40.0
52-53	34.954485300408905	37.0	33.0	40.0	27.0	40.0
54-55	34.83824253844766	37.0	33.0	40.0	27.0	40.0
56-57	34.644672364619055	37.0	33.0	40.0	27.0	40.0
58-59	34.4508711927206	37.0	33.0	40.0	27.0	40.0
60-61	34.18968254265256	37.0	33.0	37.0	27.0	40.0
62-63	33.972445145187734	37.0	33.0	37.0	27.0	40.0
64-65	33.772070224093646	37.0	33.0	37.0	27.0	40.0
66-67	33.55312543110142	37.0	33.0	37.0	27.0	40.0
68-69	33.34732111980652	37.0	33.0	37.0	27.0	40.0
70-71	33.121638769709044	37.0	33.0	37.0	24.5	38.5
72-73	32.883883793682315	37.0	33.0	37.0	22.0	37.0
74-75	32.659031794894126	37.0	33.0	37.0	22.0	37.0
76-77	32.454783658740666	33.0	33.0	37.0	22.0	37.0
78-79	32.25183922432277	33.0	33.0	37.0	22.0	37.0
80-81	32.09288929085804	33.0	33.0	37.0	22.0	37.0
82-83	31.9352186070136	33.0	33.0	37.0	22.0	37.0
84-85	31.766134356166024	33.0	33.0	37.0	22.0	37.0
86-87	31.558096014568868	33.0	33.0	37.0	22.0	37.0
88-89	31.413032713783355	33.0	33.0	37.0	22.0	37.0
90-91	31.308659816558954	33.0	33.0	37.0	22.0	37.0
92-93	31.211470227869903	33.0	30.0	37.0	22.0	37.0
94-95	31.07300461809028	33.0	27.0	37.0	22.0	37.0
96-97	30.959480293466207	33.0	27.0	37.0	15.0	37.0
98-99	30.824323203722095	33.0	27.0	37.0	15.0	37.0
100	30.735196944565736	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	5.0
7	147.0
8	1031.0
9	2338.0
10	4026.0
11	7222.0
12	12469.0
13	19880.0
14	26692.0
15	30896.0
16	36820.0
17	44308.0
18	53710.0
19	64538.0
20	77089.0
21	91938.0
22	106411.0
23	116463.0
24	122145.0
25	134555.0
26	155436.0
27	185210.0
28	226909.0
29	282787.0
30	352726.0
31	437110.0
32	557709.0
33	733197.0
34	962835.0
35	1296932.0
36	1900488.0
37	2645754.0
38	1066596.0
39	1302.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.600269233976576	19.40140539455031	13.054741972760056	24.943583398713052
2	15.438665653400543	21.221130869309	38.28602739987071	25.054176077419747
3	17.57303766504056	26.379488331200214	29.9974776830243	26.049996320734937
4	12.498457036801172	17.651660628232513	37.69720898693226	32.15267334803406
5	13.792204405096278	37.69707411486405	33.90574332161174	14.604978158427933
6	30.752604288515613	37.52658735053787	17.67677472319465	14.044033637751868
7	26.807781481875747	32.39175384231026	22.646181039256035	18.15428363655796
8	26.50198576684929	33.62262681234769	20.343841783719014	19.531545637084
9	26.518884819040593	14.763640477171638	20.311213686597863	38.406261017189905
10-11	24.781942973446334	25.592507878537102	27.670306850973013	21.955242297043547
12-13	25.931720482281857	23.34330538838835	27.179959588426918	23.545014540902876
14-15	23.661227810846533	24.26177271847943	25.07013558390922	27.006863886764815
16-17	23.254903945441217	26.962722189428128	25.72404111301827	24.05833275211238
18-19	23.442363497530316	26.289744445202857	27.66979946317396	22.598092594092872
20-21	24.716782954619735	25.641841326835628	26.794034719119075	22.847340999425562
22-23	24.348151708675093	25.407342312241266	26.555670381840713	23.688835597242928
24-25	23.814481318868197	25.850534356411753	26.715638473253296	23.619345851466754
26-27	23.90699251275435	25.836197705142087	26.628791238287018	23.62801854381654
28-29	23.572929307781283	25.738475089952406	27.107731694985315	23.580863907280992
30-31	23.954198049145283	25.619640381332083	26.95465492508132	23.471506644441313
32-33	23.726576456038593	25.831692953165035	26.452426706169092	23.98930388462728
34-35	23.973873762640572	25.862944682925143	26.878304613680413	23.284876940753872
36-37	24.08438174468753	25.608406180744968	26.56280618993466	23.744405884632844
38-39	24.042467930648286	25.502637055892208	26.737211680910494	23.717683332549015
40-41	23.89786607664601	25.537335680957924	26.928453017682152	23.636345224713914
42-43	24.18892566632329	25.5600587701416	26.495496288996595	23.75551927453851
44-45	23.90174103505575	25.87939349965696	26.301002159337568	23.917863305949727
46-47	23.811702069263674	25.808359180158146	26.34864682351069	24.03129192706749
48-49	24.31664382057418	25.868702932726784	26.04483053983771	23.76982270686132
50-51	23.643683964914864	26.19098930166216	25.887586839712956	24.27773989371002
52-53	23.7103540963897	26.0309631706891	25.748474652154012	24.51020808076719
54-55	23.868079689911458	25.943090444589632	25.7809750465951	24.407854818903807
56-57	24.070755831468027	25.711730908681428	25.57597701722296	24.64153624262758
58-59	23.606016802302904	25.723689056185773	26.19923379403103	24.471060347480293
60-61	23.86731848493163	25.869563997096705	25.904456102457	24.358661415514668
62-63	23.615520726943874	25.746149949821845	25.800178803398467	24.838150519835818
64-65	23.363707288379892	25.676463201311893	25.981068853745654	24.97876065656256
66-67	23.99615024652915	25.576297820412197	25.825238168625052	24.602313764433596
68-69	23.771062940651174	25.261945994857676	25.984444775107285	24.982546289383862
70-71	23.368853424553258	25.377066790047177	26.005044368928694	25.24903541647087
72-73	23.849715720939503	25.401273232027833	26.00193814454384	24.747072902488824
74-75	23.932974132331243	25.495936928657503	25.903164738556395	24.66792420045486
76-77	23.612349133023194	25.54431820767172	25.894727844617137	24.948604814687947
78-79	23.68081901233186	25.62781550160346	25.85992773740793	24.831437748656747
80-81	23.433451646148505	25.523856868780793	26.16787888929548	24.87481259577523
82-83	23.649791406118336	25.37432313780583	26.07348038050613	24.9024050755697
84-85	23.655357665698702	25.59189233956388	25.63652759329187	25.116222401445544
86-87	23.33091028104926	25.458528233251265	25.991533109961168	25.219028375738304
88-89	23.530835654327202	24.999580050122667	26.083033723017053	25.386550572533086
90-91	23.605008232400873	25.22002683971918	25.9137590729495	25.261205854930452
92-93	23.53780662669312	25.143219790202448	25.94177784720101	25.377195735903424
94-95	22.99302491009977	25.139109915130035	26.415457340497824	25.45240783427237
96-97	23.52773822223536	25.07656575626665	26.012144289829937	25.383551731668053
98-99	23.273152575304763	25.168771180122874	25.94881719621634	25.609259048356027
100	23.23956186359452	25.07990963604617	25.914494456783306	25.766034043576003
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1714.0
1	1644.0
2	2020.5
3	2482.0
4	2084.5
5	1748.0
6	2203.0
7	4147.0
8	6797.0
9	6721.0
10	4325.5
11	2846.0
12	2586.0
13	2612.5
14	2887.5
15	3527.0
16	4072.5
17	4679.5
18	5688.5
19	6937.5
20	8603.0
21	10937.5
22	14274.0
23	18545.0
24	23312.0
25	29453.0
26	38657.0
27	48849.5
28	58453.0
29	72141.5
30	88110.0
31	104028.0
32	121395.0
33	138264.5
34	156922.5
35	173385.0
36	189707.5
37	212151.5
38	230928.5
39	248576.5
40	262699.5
41	272820.0
42	287175.0
43	312949.5
44	348572.5
45	388550.5
46	431275.0
47	468132.5
48	499126.0
49	518442.5
50	540498.5
51	543002.0
52	517830.0
53	508593.0
54	496025.5
55	464768.0
56	433228.5
57	396171.0
58	363472.0
59	331510.0
60	280476.5
61	224498.0
62	180455.5
63	142656.0
64	106031.0
65	78847.5
66	60001.0
67	44868.5
68	34815.5
69	28680.5
70	24728.0
71	21375.0
72	18471.5
73	17824.5
74	14592.5
75	10930.5
76	8110.5
77	5715.5
78	4186.5
79	2692.0
80	1791.5
81	1253.5
82	821.0
83	515.5
84	342.5
85	221.0
86	130.0
87	84.0
88	57.5
89	30.5
90	21.5
91	15.0
92	8.0
93	8.5
94	6.5
95	3.0
96	1.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0494485558963448
2	0.02506448129111251
3	0.022189763043268595
4	0.022921200230589828
5	0.015998062201758614
6	0.025719372726272224
7	0.04954211181565333
8	0.006421338097994552
9	0.004073935031707802
10-11	0.0021815539366034473
12-13	0.0012842676195989106
14-15	0.00228361493948548
16-17	0.006923138028831212
18-19	0.005158333187329399
20-21	0.008594386951024498
22-23	0.019013114328565325
24-25	0.025681099850191458
26-27	0.028504787596594364
28-29	0.045485186951092535
30-31	0.04278482291650543
32-33	0.04090094690497457
34-35	0.04875964412689108
36-37	0.045663793706136095
38-39	0.05529154831134118
40-41	0.052910124910760414
42-43	0.05791111405198001
44-45	0.05824281231134661
46-47	0.0625889100174065
48-49	0.0652382435505526
50-51	0.03384172753896732
52-53	2.3814234005807612E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	5.740931412114334E-4
64-65	1.1056608645553533E-4
66-67	3.231931757931033E-4
68-69	0.0012374896599446454
70-71	7.654575216152446E-5
72-73	3.146880922196006E-4
74-75	3.827287608076223E-5
76-77	5.5283043227767664E-5
78-79	0.0
80-81	1.2757625360254078E-5
82-83	1.573440461098003E-4
84-85	0.0012885201613856618
86-87	0.003950611319892013
88-89	0.002708869118160616
90-91	0.004014399446693283
92-93	0.0041037028242150615
94-95	0.0028449504553366596
96-97	0.002521757279543556
98-99	0.004107955366001813
100	0.0028151826628293997
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1757674E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.74266644496501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.2573425966082	31.414421495446724
2	12.815364932724052	10.698950075144031
3	4.575982777362445	5.730411682000353
4	2.2413959792839426	3.742473789373413
5	1.242694626545068	2.5936693644410567
6	0.7612307159779993	1.906547991883891
7	0.5185047095380876	1.5150638398274299
8	0.3748426201029084	1.2517544368249955
9	0.28953302760652666	1.087729253656208
>10	1.6318414588199621	12.569432343924325
>50	0.1480590981735017	4.299077506588
>100	0.11613339738149311	9.904568107315466
>500	0.017014335358853598	4.978448677018921
>1k	0.009805048265964357	7.268948733358204
>5k	1.6978416744088074E-4	0.48734546560525355
>10k+	8.489208372044037E-5	0.5511572375918496
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17869	0.15197733837492006	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	17735	0.1508376571760707	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	16533	0.14061454672072043	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.7010167147005436E-5	0.0
2	0.0	8.505083573502718E-6	0.0	1.7010167147005436E-5	0.0
3	0.0	8.505083573502718E-6	0.0	1.7010167147005436E-5	0.0
4	0.0	1.7010167147005436E-5	0.0	1.7010167147005436E-5	0.0
5	0.0	2.5515250720508156E-5	0.0	3.402033429401087E-5	0.0
6	0.0	2.5515250720508156E-5	0.0	3.402033429401087E-5	0.0
7	8.505083573502718E-6	2.5515250720508156E-5	0.0	5.953558501451903E-5	0.0
8	8.505083573502718E-6	2.5515250720508156E-5	0.0	5.953558501451903E-5	0.0
9	1.7010167147005436E-5	5.103050144101631E-5	0.0	5.953558501451903E-5	0.0
10-11	1.7010167147005436E-5	5.103050144101631E-5	0.0	9.35559193085299E-5	0.0
12-13	1.7010167147005436E-5	5.103050144101631E-5	0.0	9.35559193085299E-5	0.0
14-15	2.9767792507259512E-5	7.654575216152446E-5	0.0	9.35559193085299E-5	4.252541786751359E-6
16-17	4.677795965426495E-5	7.654575216152446E-5	0.0	1.1907117002903806E-4	2.5515250720508156E-5
18-19	5.953558501451903E-5	7.654575216152446E-5	0.0	1.65849129683303E-4	4.252541786751359E-5
20-21	6.804066858802174E-5	7.654575216152446E-5	0.0	2.4664742363157883E-4	4.252541786751359E-5
22-23	7.654575216152446E-5	8.079829394827583E-5	0.0	9.270541095117962E-4	5.9535585014519024E-5
24-25	8.930337752177854E-5	9.780846109528127E-5	0.0	0.002364413233433756	8.505083573502718E-5
26-27	1.2757625360254078E-4	1.1056608645553534E-4	0.0	0.003631670685885661	8.505083573502718E-5
28-29	1.360813371760435E-4	1.1056608645553534E-4	0.0	0.011111891688781302	8.505083573502718E-5
30-31	1.7010167147005437E-4	1.4458642074954622E-4	0.0	0.033025239515911055	9.35559193085299E-5
32-33	1.871118386170598E-4	1.4458642074954622E-4	0.0	0.07567823363702719	1.0206100288203262E-4
34-35	1.9986946397731388E-4	1.6159658789655166E-4	0.0	0.13264103087056164	1.1907117002903806E-4
36-37	2.1262708933756795E-4	1.7860675504355707E-4	0.0	0.2227991692914772	1.1907117002903806E-4
38-39	2.296372564845734E-4	1.871118386170598E-4	0.0	0.36280134999490543	1.1907117002903806E-4
40-41	2.296372564845734E-4	2.0412200576406525E-4	0.0	0.5256014072171077	1.2757625360254078E-4
42-43	2.4664742363157883E-4	2.0412200576406525E-4	0.0	0.6820269043009697	1.4033387896279485E-4
44-45	2.7641521613883834E-4	2.0412200576406525E-4	0.0	0.8688665802436775	1.4458642074954622E-4
46-47	2.806677579255897E-4	2.0412200576406525E-4	0.0	1.0776153514717282	1.4883896253629756E-4
48-49	2.806677579255897E-4	2.0412200576406525E-4	0.0	1.299997771668104	1.573440461098003E-4
50-51	2.806677579255897E-4	2.1262708933756795E-4	0.0	1.543264424579215	1.6159658789655166E-4
52-53	2.8917284149909244E-4	2.1262708933756795E-4	0.0	1.7748408401185474	1.9136438040381117E-4
54-55	2.9342538328584375E-4	2.2113217291107068E-4	0.0	2.0154921798308068	2.2538471469782205E-4
56-57	3.019304668593465E-4	2.296372564845734E-4	0.0	2.2897428522001886	2.3388979827132476E-4
58-59	3.146880922196006E-4	2.296372564845734E-4	0.0	2.5860046808577954	2.4239488184482746E-4
60-61	3.146880922196006E-4	2.3388979827132476E-4	0.0	2.885609007359789	2.508999654183302E-4
62-63	3.146880922196006E-4	2.3814234005807612E-4	0.0	3.1959382442479694	2.6365759077858424E-4
64-65	3.146880922196006E-4	2.4664742363157883E-4	8.505083573502718E-6	3.52434503627163	2.6365759077858424E-4
66-67	3.146880922196006E-4	2.5940504899183293E-4	8.505083573502718E-6	3.881490505690156	2.6365759077858424E-4
68-69	3.1894063400635195E-4	2.679101325653356E-4	8.505083573502718E-6	4.265137815523716	2.679101325653356E-4
70-71	3.231931757931033E-4	2.806677579255897E-4	8.505083573502718E-6	4.683749523927947	2.72162674352087E-4
72-73	3.231931757931033E-4	2.8917284149909244E-4	8.505083573502718E-6	5.100001071640531	3.0618300864609785E-4
74-75	3.231931757931033E-4	2.8917284149909244E-4	8.505083573502718E-6	5.521206830534679	3.0618300864609785E-4
76-77	3.231931757931033E-4	3.019304668593465E-4	8.505083573502718E-6	5.979486248725726	3.0618300864609785E-4
78-79	3.231931757931033E-4	3.0618300864609785E-4	8.505083573502718E-6	6.458879536887993	3.0618300864609785E-4
80-81	3.231931757931033E-4	3.0618300864609785E-4	8.505083573502718E-6	6.969541764808243	3.104355504328492E-4
82-83	3.231931757931033E-4	3.104355504328492E-4	8.505083573502718E-6	7.512085298503768	3.231931757931033E-4
84-85	3.231931757931033E-4	3.231931757931033E-4	8.505083573502718E-6	8.051039686931276	3.231931757931033E-4
86-87	3.231931757931033E-4	3.31698259366606E-4	8.505083573502718E-6	8.610703953860263	3.231931757931033E-4
88	3.231931757931033E-4	4.082440115281305E-4	8.505083573502718E-6	9.046678790379797	3.231931757931033E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	41185	0.0	21.160057	1
GTACTAG	5065	0.0	20.880901	1
GTATAAG	5525	0.0	20.333485	1
TAGGCAT	7090	0.0	18.95669	5
TAGTACT	6590	0.0	18.684309	4
GTACATG	62240	0.0	18.435053	1
CTAGTAC	6940	0.0	17.877985	3
CAACGCA	44820	0.0	17.793144	5
TACATGG	62110	0.0	17.730007	2
ATCAACG	45305	0.0	17.510576	3
AACGCAG	47185	0.0	17.359013	6
TATCAAC	49960	0.0	17.253414	2
ACATGGG	60320	0.0	16.829319	3
CTAGGCA	8295	0.0	16.770132	4
CATGGGG	24035	0.0	16.678848	4
ACGCAGA	48655	0.0	16.35052	7
TCAACGC	49710	0.0	16.043505	4
GTATAAA	15140	0.0	15.896088	1
GTGATCG	3985	0.0	15.799704	8
TGATCGC	3945	0.0	15.720761	9
>>END_MODULE