FastQCFastQC Report
Fri 27 May 2016
ERR765472_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765472_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13649666
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT517750.37931331067001933No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA504710.3697599633573452No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT218690.16021637452520815No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG196220.14375443325865994No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC147680.10819312355335288No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC147650.10817114499358446No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT138990.10182666740709992No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA54100.022.9387171
GTATAAG47850.020.8265021
GTACTAG37700.020.4486661
GTTATAT42300.019.4473361
TATATAC75600.018.5268333
GTACAAG65600.017.9859051
TGATCGC30400.017.6190329
ATAAGGT39700.017.5217693
GTATTAC25600.017.4441
CGTTAAC11100.017.3629421
TCTAACG18400.017.1151712
GTACATG1298750.016.9170881
TTACGTA9150.016.4368324
TACATGG1288750.016.3502462
ATAGATC63100.016.3125363
TAGTACT47300.016.295664
GTACATA35550.016.264021
CTAACGC20200.016.0547833
AGGATAG38600.015.8272515
CTAGTAC47000.015.8003243
Produced by FastQC (version 0.11.5)