##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765472_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13649666
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74942390531754	33.0	33.0	33.0	27.0	33.0
2	31.66115647078837	33.0	33.0	33.0	27.0	33.0
3	31.605476427042245	33.0	33.0	33.0	27.0	33.0
4	35.22379140998761	37.0	37.0	37.0	33.0	37.0
5	35.4322606135564	37.0	37.0	37.0	33.0	37.0
6	35.44391181439897	37.0	37.0	37.0	33.0	37.0
7	35.306726992440694	37.0	37.0	37.0	33.0	37.0
8	35.282363685675534	37.0	37.0	37.0	33.0	37.0
9	34.97395276924725	37.0	37.0	37.0	33.0	37.0
10-11	34.99338837301953	37.0	37.0	37.0	33.0	37.0
12-13	34.96912455586825	37.0	37.0	37.0	33.0	37.0
14-15	36.27839758130345	37.0	37.0	40.0	30.0	40.0
16-17	36.53017758822817	37.0	37.0	40.0	33.0	40.0
18-19	36.35466673690037	37.0	37.0	40.0	33.0	40.0
20-21	36.353158091926936	37.0	37.0	40.0	33.0	40.0
22-23	36.54459479081759	37.0	37.0	40.0	33.0	40.0
24-25	36.565787910121756	37.0	37.0	40.0	33.0	40.0
26-27	36.2379687532281	37.0	37.0	40.0	30.0	40.0
28-29	36.11844615831626	37.0	37.0	40.0	27.0	40.0
30-31	35.92111191585201	37.0	37.0	40.0	27.0	40.0
32-33	35.88242239773486	37.0	37.0	40.0	27.0	40.0
34-35	35.831186308881115	37.0	37.0	40.0	27.0	40.0
36-37	35.60102543901074	37.0	37.0	40.0	27.0	40.0
38-39	35.468211969435735	37.0	35.0	40.0	27.0	40.0
40-41	35.61745100576087	37.0	37.0	40.0	27.0	40.0
42-43	35.53745956128157	37.0	35.0	40.0	27.0	40.0
44-45	35.495506410193485	37.0	33.0	40.0	27.0	40.0
46-47	35.45619651059594	37.0	33.0	40.0	27.0	40.0
48-49	35.29035875310062	37.0	33.0	40.0	27.0	40.0
50-51	35.147757974444204	37.0	33.0	40.0	27.0	40.0
52-53	35.03979811667186	37.0	33.0	40.0	27.0	40.0
54-55	34.91637425413926	37.0	33.0	40.0	27.0	40.0
56-57	34.72120951530975	37.0	33.0	40.0	27.0	40.0
58-59	34.539893137311935	37.0	33.0	40.0	27.0	40.0
60-61	34.259425029154556	37.0	33.0	37.0	27.0	40.0
62-63	34.03042623900102	37.0	33.0	37.0	27.0	40.0
64-65	33.836904983609124	37.0	33.0	37.0	27.0	40.0
66-67	33.61710235254108	37.0	33.0	37.0	27.0	40.0
68-69	33.40826925728439	37.0	33.0	37.0	27.0	40.0
70-71	33.156239061087646	37.0	33.0	37.0	27.0	38.5
72-73	32.90858699399678	37.0	33.0	37.0	22.0	37.0
74-75	32.68416593490273	37.0	33.0	37.0	22.0	37.0
76-77	32.468749381853	33.0	33.0	37.0	22.0	37.0
78-79	32.265082163915224	33.0	33.0	37.0	22.0	37.0
80-81	32.11320093839659	33.0	33.0	37.0	22.0	37.0
82-83	31.970792801816543	33.0	33.0	37.0	22.0	37.0
84-85	31.802349339536953	33.0	33.0	37.0	22.0	37.0
86-87	31.58738558144939	33.0	33.0	37.0	22.0	37.0
88-89	31.432351714686646	33.0	33.0	37.0	22.0	37.0
90-91	31.336668494306018	33.0	33.0	37.0	22.0	37.0
92-93	31.23663817121972	33.0	33.0	37.0	22.0	37.0
94-95	31.096922554735038	33.0	27.0	37.0	22.0	37.0
96-97	30.99033309679519	33.0	27.0	37.0	15.0	37.0
98-99	30.852863835642573	33.0	27.0	37.0	15.0	37.0
100	30.756473088792063	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	10.0
7	183.0
8	1179.0
9	2517.0
10	4122.0
11	7203.0
12	12929.0
13	21544.0
14	31569.0
15	38535.0
16	44099.0
17	51647.0
18	61484.0
19	73327.0
20	86635.0
21	102986.0
22	119809.0
23	126942.0
24	130491.0
25	142922.0
26	167116.0
27	202227.0
28	248471.0
29	314367.0
30	394671.0
31	492564.0
32	634720.0
33	844865.0
34	1118429.0
35	1524616.0
36	2273111.0
37	3169674.0
38	1203447.0
39	1254.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.5596403680172	18.48653725579705	12.763348217093265	25.190474159092485
2	15.682998606140997	20.75334484757649	37.72681902410524	25.83683752217728
3	18.06106005548756	25.948350889884214	29.249648796925666	26.74094025770256
4	12.592897919732915	17.47281458740716	36.41827815278057	33.51600934007936
5	14.135349188103003	37.2446646810591	33.346197674834386	15.273788456003512
6	31.91789467991487	36.12011054482524	17.479121887812376	14.482872887447515
7	27.658563269366525	31.640741331738763	22.040244782639192	18.66045061625552
8	27.430548864918276	32.54852188548464	20.067132440036485	19.953796809560608
9	26.97222831337734	14.571909670054115	19.648157459563134	38.80770455700541
10-11	25.09888266390327	25.255593026923666	27.02745314266735	22.61807116650571
12-13	26.644317858587836	22.96867705203067	26.429262322660595	23.957742766720898
14-15	24.354720566344586	23.725141504823892	24.502090134935283	27.41804779389624
16-17	23.696442263203725	26.44241340112487	25.559468828727706	24.3016755069437
18-19	24.34875347091476	25.481414901993077	27.152494664520034	23.01733696257213
20-21	25.44822873035792	24.83394020832734	26.307112389316828	23.41071867199791
22-23	24.59527009569634	24.884133113448208	25.964972405178937	24.555624385676516
24-25	24.10114644217689	25.449729519434182	26.057240437603962	24.391883600784965
26-27	24.53384178884159	25.065090686164677	25.97982840029652	24.42123912469722
28-29	24.202995198934705	24.99080058763365	26.635858178831885	24.170346034599756
30-31	24.47427126146203	25.060303033623445	26.45401671286653	24.011408992048
32-33	24.019463721219495	25.16529149279946	26.00605819921331	24.80918658676773
34-35	24.375780203738586	25.27971158399747	26.549418382415514	23.795089829848425
36-37	24.731201434769957	25.09608371172332	25.957178766075995	24.215536087430724
38-39	24.771059889748393	24.78627009162296	26.008946750695884	24.433723267932763
40-41	24.34377084826602	25.23443424455752	26.19373989529683	24.22805501187963
42-43	25.030561391681093	24.87116164662539	25.886687196454634	24.211589765238887
44-45	24.63042205712195	25.204303980645875	25.52360700815112	24.641666954081053
46-47	24.23086149985091	25.18971221026715	26.16750197793473	24.411924311947203
48-49	24.828148617709534	25.45362222045383	25.524956099479184	24.193273062357452
50-51	24.046742547466785	25.744439839189937	25.358918842423456	24.849898770919822
52-53	24.007977652433716	25.581665055450774	25.124227730869915	25.286129561245602
54-55	24.303671606323554	25.535405042145353	25.122438893376586	25.038484458154507
56-57	24.445510241789066	24.985582064791913	25.027605071069136	25.541302622349882
58-59	24.16474146693406	24.967101759119966	25.63501920120243	25.233137572743537
60-61	24.295924164005186	25.194484612297472	25.391716544566002	25.11787467913134
62-63	23.689668761309413	25.259398034357535	25.4205970980945	25.630336106238556
64-65	23.70986205135738	25.14817784638355	25.46792608892026	25.674034013338808
66-67	24.37051342689787	25.173968264015635	25.22891486471121	25.226603444375286
68-69	24.532720456699128	24.799671402159703	25.240232956322284	25.427375184818885
70-71	23.72392338988944	24.994980644390385	25.44948645426016	25.831609511460012
72-73	24.44883528063744	25.038477205259625	25.34289182718372	25.169795686919215
74-75	24.577783454760652	25.167032542677525	25.201612157313363	25.053571845248456
76-77	24.23606766017411	25.177102468051483	25.60990829474841	24.976921577025998
78-79	24.376117495333492	25.231409516958493	25.3311848557754	25.061288131932614
80-81	23.859322529036888	25.15762935530457	25.76276153348114	25.220286582177398
82-83	24.06354028617386	25.141118195832206	25.131260792638514	25.66408072535542
84-85	24.01641170723566	25.363268149011343	24.845275745686234	25.77504439806676
86-87	24.165269997884117	24.863093978640713	25.11983169054411	25.85180433293106
88-89	24.17771107850144	24.47545843572394	25.403083163791962	25.943747321982663
90-91	24.344793156913724	24.78528536878049	25.21475492673067	25.655166547575114
92-93	24.05509472900356	24.92677197533091	25.132096176566648	25.88603711909888
94-95	23.4921832961903	24.950434138809026	25.470308343010593	26.087074221990076
96-97	24.14060161293844	24.771337498701406	25.08905536620214	25.999005522158015
98-99	24.121120784315305	24.974323403315346	24.982162746598174	25.922393065771175
100	23.688150583767058	25.112833790500304	25.157451528578793	26.04156409715384
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1194.0
1	1117.5
2	1539.0
3	2089.0
4	1921.5
5	1838.5
6	2335.0
7	4021.0
8	6614.5
9	6756.0
10	4495.0
11	3108.5
12	2835.5
13	2932.0
14	3275.5
15	3863.5
16	4575.0
17	5441.0
18	6519.5
19	7985.0
20	10002.0
21	12402.5
22	15589.5
23	20371.5
24	26437.5
25	33308.0
26	40905.5
27	47955.0
28	55602.5
29	67328.5
30	81162.0
31	94907.5
32	111631.5
33	128983.0
34	147412.0
35	164012.0
36	180428.0
37	202576.5
38	216204.0
39	224751.0
40	239904.5
41	256604.0
42	276727.0
43	313008.0
44	359211.0
45	407281.5
46	467314.0
47	523200.0
48	573030.0
49	625423.0
50	721448.0
51	744956.5
52	670067.0
53	631020.5
54	601367.0
55	566476.0
56	530521.0
57	496684.0
58	472983.5
59	435379.5
60	372587.0
61	301490.5
62	240723.0
63	188335.5
64	141605.5
65	110376.5
66	87308.0
67	65495.5
68	48898.5
69	39280.0
70	33728.0
71	29541.5
72	25242.0
73	24836.5
74	20846.0
75	15340.5
76	11380.5
77	8003.5
78	5768.0
79	3581.0
80	2217.5
81	1559.0
82	1050.0
83	689.5
84	481.0
85	311.0
86	210.0
87	117.5
88	62.0
89	42.5
90	37.5
91	28.0
92	15.5
93	12.0
94	11.5
95	8.5
96	3.5
97	1.0
98	2.5
99	2.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04841144098324457
2	0.024755184485832842
3	0.022000538328190596
4	0.02271850461395905
5	0.015546167942863951
6	0.025180103308022336
7	0.04912208108242355
8	0.006044103936316097
9	0.004022076437621258
10-11	0.002062321524936947
12-13	0.0012344624403263786
14-15	0.0022564654695580097
16-17	0.0070404653124845696
18-19	0.005175950825463422
20-21	0.008743803694537288
22-23	0.018608513937264107
24-25	0.025579380477148674
26-27	0.028546486045885666
28-29	0.04492051307336018
30-31	0.04297907362714956
32-33	0.040385603574475744
34-35	0.04831620055758141
36-37	0.04560184842618127
38-39	0.05499401963388702
40-41	0.05270824941797111
42-43	0.05760214205973978
44-45	0.058041713255108214
46-47	0.06259127512717161
48-49	0.06507485238100331
50-51	0.03387994988302278
52-53	2.674058105158031E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.080734869263468E-4
64-65	1.538499183789552E-4
66-67	3.6630932947370287E-4
68-69	0.001131895828073742
70-71	4.029402624210732E-5
72-73	1.904808513263255E-4
74-75	4.029402624210732E-5
76-77	8.425114577895166E-5
78-79	3.663093294737029E-6
80-81	2.56416530631592E-5
82-83	1.9414394462106252E-4
84-85	0.001036655402410579
86-87	0.003824269399705458
88-89	0.002509218906894865
90-91	0.0036850718545054506
92-93	0.0038096170265265097
94-95	0.0029487901022633082
96-97	0.0021392464841264247
98-99	0.0041283061431686315
100	0.0028279080235369862
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3649666E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.26309545814334
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.6643726587598	25.23969432271332
2	12.858209454840313	8.811241159439898
3	4.789001412263812	4.922580376127347
4	2.3598977850779	3.2342961232634053
5	1.3670857465906774	2.342029471745177
6	0.889952199048969	1.8295510289519645
7	0.6353474611153743	1.523827949649956
8	0.45507033376422856	1.247369462874633
9	0.3156274916296988	0.9732937387430436
>10	2.20380835846127	14.559695388589308
>50	0.23987186853606823	5.744776569264306
>100	0.18540452846546335	12.674766951521299
>500	0.021635594943111593	5.104520137295715
>1k	0.01391002708004287	8.789729428232551
>5k	5.590829329637803E-4	1.1922103201746732
>10k+	2.4599649050406326E-4	1.8104175714135646
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	51775	0.37931331067001933	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	50471	0.3697599633573452	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21869	0.16021637452520815	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	19622	0.14375443325865994	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	14768	0.10819312355335288	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	14765	0.10817114499358446	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	13899	0.10182666740709992	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	1.4652373178948116E-5	0.0	0.0	0.0
5	0.0	1.4652373178948116E-5	0.0	1.4652373178948116E-5	0.0
6	0.0	1.4652373178948116E-5	0.0	1.4652373178948116E-5	0.0
7	0.0	1.4652373178948116E-5	0.0	1.4652373178948116E-5	0.0
8	0.0	1.4652373178948116E-5	0.0	1.4652373178948116E-5	0.0
9	0.0	4.3957119536844344E-5	0.0	1.4652373178948116E-5	0.0
10-11	0.0	4.3957119536844344E-5	0.0	6.593567930526652E-5	1.4652373178948116E-5
12-13	0.0	5.1283306126318403E-5	0.0	7.326186589474057E-5	4.3957119536844344E-5
14-15	0.0	5.1283306126318403E-5	0.0	7.326186589474057E-5	4.3957119536844344E-5
16-17	0.0	5.1283306126318403E-5	0.0	1.0256661225263681E-4	4.7620212831581374E-5
18-19	7.326186589474058E-6	5.1283306126318403E-5	0.0	1.3187135861053302E-4	5.860949271579246E-5
20-21	2.1978559768422172E-5	5.494639942105543E-5	0.0	2.6374271722106604E-4	6.593567930526652E-5
22-23	2.5641653063159202E-5	6.593567930526651E-5	0.0	0.0012161469738526936	7.326186589474057E-5
24-25	2.930474635789623E-5	7.326186589474057E-5	0.0	0.0030989769273475263	7.69249591894776E-5
26-27	3.6630932947370284E-5	7.326186589474057E-5	0.0	0.0055056292219897544	8.425114577895166E-5
28-29	4.3957119536844344E-5	7.326186589474057E-5	0.0	0.016381353214063994	9.157733236842572E-5
30-31	5.860949271579246E-5	9.524042566316275E-5	0.0	0.05118806570065523	9.524042566316275E-5
32-33	7.326186589474057E-5	1.0622970554737384E-4	0.0	0.1137060789619321	1.0256661225263681E-4
34-35	8.058805248421463E-5	1.2454517202105899E-4	0.0	0.19679602416645214	1.0989279884211087E-4
36-37	8.058805248421463E-5	1.391975452000071E-4	0.0	0.324491456420985	1.0989279884211087E-4
38-39	8.058805248421463E-5	1.391975452000071E-4	0.0	0.5206171345145002	1.1721898543158493E-4
40-41	8.058805248421463E-5	1.4652373178948114E-4	0.0	0.7591834115208387	1.2454517202105899E-4
42-43	8.791423907368869E-5	1.5018682508421817E-4	0.0	0.9767638270416288	1.3553445190527007E-4
44-45	8.791423907368869E-5	1.6483919826316629E-4	0.0	1.2287406885999994	1.4652373178948114E-4
46-47	8.791423907368869E-5	1.8315466473685143E-4	0.0	1.5123044036388875	1.4652373178948114E-4
48-49	8.791423907368869E-5	1.8315466473685143E-4	0.0	1.8069160080547024	1.6117610496842926E-4
50-51	9.157733236842572E-5	2.0513322450527361E-4	0.0	2.12099329023875	1.6483919826316629E-4
52-53	9.524042566316275E-5	2.0513322450527361E-4	0.0	2.4433345108957245	1.8681775803158846E-4
54-55	9.524042566316275E-5	2.0513322450527361E-4	0.0	2.7651848770512037	2.0879631780001064E-4
56-57	9.524042566316275E-5	2.1978559768422173E-4	0.0	3.125233980084201	2.161225043894847E-4
58-59	9.524042566316275E-5	2.1978559768422173E-4	0.0	3.5216063162278113	2.271117842736958E-4
60-61	9.524042566316275E-5	2.1978559768422173E-4	0.0	3.919462205155789	2.271117842736958E-4
62-63	9.524042566316275E-5	2.1978559768422173E-4	0.0	4.323171717168757	2.3443797086316985E-4
64-65	9.524042566316275E-5	2.4909034404211797E-4	0.0	4.745738833463031	2.3443797086316985E-4
66-67	9.524042566316275E-5	2.5641653063159203E-4	0.0	5.207178695801055	2.454272507473809E-4
68-69	9.524042566316275E-5	2.674058105158031E-4	0.0	5.70919464256488	2.4909034404211797E-4
70-71	9.524042566316275E-5	2.857212769894882E-4	0.0	6.275952100219888	2.4909034404211797E-4
72-73	9.524042566316275E-5	2.857212769894882E-4	0.0	6.815357240243094	2.7106890381054015E-4
74-75	9.524042566316275E-5	2.9671055687369933E-4	0.0	7.353146956123322	2.820581836947512E-4
76-77	9.524042566316275E-5	3.1868911664212146E-4	0.0	7.923578496352951	2.9671055687369933E-4
78-79	9.524042566316275E-5	3.2967839652633257E-4	0.0	8.526241594482972	3.0037365016843633E-4
80-81	9.524042566316275E-5	3.2967839652633257E-4	0.0	9.16823898841188	3.1136293005264745E-4
82-83	9.524042566316275E-5	3.3700458311580663E-4	0.0	9.846149349002385	3.2967839652633257E-4
84-85	9.524042566316275E-5	3.443307697052807E-4	0.0	10.50962712201163	3.3700458311580663E-4
86-87	9.524042566316275E-5	3.5165695629475475E-4	0.0	11.195398480812646	3.479938630000177E-4
88	9.524042566316275E-5	3.589831428842288E-4	0.0	11.726272276552407	3.589831428842288E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATAGA	5410	0.0	22.938717	1
GTATAAG	4785	0.0	20.826502	1
GTACTAG	3770	0.0	20.448666	1
GTTATAT	4230	0.0	19.447336	1
TATATAC	7560	0.0	18.526833	3
GTACAAG	6560	0.0	17.985905	1
TGATCGC	3040	0.0	17.619032	9
ATAAGGT	3970	0.0	17.521769	3
GTATTAC	2560	0.0	17.444	1
CGTTAAC	1110	0.0	17.362942	1
TCTAACG	1840	0.0	17.115171	2
GTACATG	129875	0.0	16.917088	1
TTACGTA	915	0.0	16.436832	4
TACATGG	128875	0.0	16.350246	2
ATAGATC	6310	0.0	16.312536	3
TAGTACT	4730	0.0	16.29566	4
GTACATA	3555	0.0	16.26402	1
CTAACGC	2020	0.0	16.054783	3
AGGATAG	3860	0.0	15.827251	5
CTAGTAC	4700	0.0	15.800324	3
>>END_MODULE