FastQCFastQC Report
Fri 27 May 2016
ERR765471_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765471_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13787656
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT591340.42889088616658266No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA567180.41136796566435946No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG212720.1542829325013621No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT178920.12976825067292078No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG178790.12967396343511906No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174990.12691787494553097No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA174810.12678732338549786No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC169770.12313187970457051No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG163280.11842477067893194No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC161600.11720628945195614No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC157770.11442844236902923No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA46850.020.7662411
GTACTAG54600.020.4010661
GTATAAG60600.019.8547531
TACATGA63150.019.1252922
GTACAAG67200.018.6740761
TAGTACT64200.018.445294
CATGGGG328000.017.5788574
GTACAAA73900.017.1082271
TAGGCAT78150.016.5348425
ACGTTAA17600.016.2827748
GTACATG1527000.016.1621861
GGTCGCC91850.015.95824394
TACATGG1500200.015.6408632
TCTAACG30000.015.508232
CTGTGCG72000.015.39860259
GTGATCG39100.015.379588
CTAGGCA89700.015.139984
CTAGTAC72200.015.1002233
CGTTAAC19350.015.0526349
GTATATA43100.014.8306021
Produced by FastQC (version 0.11.5)