##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765471_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	13787656
Sequences flagged as poor quality	0
Sequence length	100
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385202241773367	33.0	33.0	33.0	27.0	33.0
2	31.285133092963736	33.0	33.0	33.0	27.0	33.0
3	31.244081009854032	33.0	33.0	33.0	27.0	33.0
4	34.77730913797095	37.0	37.0	37.0	27.0	37.0
5	35.02672187353674	37.0	37.0	37.0	33.0	37.0
6	35.04501396031348	37.0	37.0	37.0	33.0	37.0
7	34.89561213305583	37.0	37.0	37.0	33.0	37.0
8	34.859661932383574	37.0	37.0	37.0	33.0	37.0
9	34.547724863457574	37.0	37.0	37.0	27.0	37.0
10-11	34.57686781567512	37.0	37.0	37.0	27.0	37.0
12-13	34.553702674334204	37.0	37.0	37.0	27.0	37.0
14-15	35.74093431834969	37.0	37.0	40.0	27.0	40.0
16-17	35.988073462233174	37.0	37.0	40.0	27.0	40.0
18-19	35.81810595651646	37.0	37.0	40.0	27.0	40.0
20-21	35.79778288637314	37.0	37.0	40.0	27.0	40.0
22-23	35.98628777074218	37.0	37.0	40.0	27.0	40.0
24-25	36.02079718989218	37.0	37.0	40.0	27.0	40.0
26-27	35.70415123498874	37.0	37.0	40.0	27.0	40.0
28-29	35.581933905226535	37.0	35.0	40.0	27.0	40.0
30-31	35.361140936501464	37.0	33.0	40.0	27.0	40.0
32-33	35.296773033791965	37.0	33.0	40.0	27.0	40.0
34-35	35.2424106026434	37.0	33.0	40.0	27.0	40.0
36-37	35.01478819169843	37.0	33.0	40.0	27.0	40.0
38-39	34.88956571008154	37.0	33.0	40.0	27.0	40.0
40-41	35.01453488540764	37.0	33.0	40.0	27.0	40.0
42-43	34.91875221575009	37.0	33.0	40.0	27.0	40.0
44-45	34.85084763501497	37.0	33.0	40.0	27.0	40.0
46-47	34.78846342699586	37.0	33.0	40.0	27.0	40.0
48-49	34.60226245128251	37.0	33.0	40.0	22.0	40.0
50-51	34.45093321156257	37.0	33.0	40.0	22.0	40.0
52-53	34.34070580960244	37.0	33.0	40.0	22.0	40.0
54-55	34.231628167978656	37.0	33.0	40.0	22.0	40.0
56-57	34.041202289932386	37.0	33.0	40.0	22.0	40.0
58-59	33.85203083105642	37.0	33.0	40.0	22.0	40.0
60-61	33.57925284761964	37.0	33.0	37.0	22.0	40.0
62-63	33.34691835943687	37.0	33.0	37.0	22.0	40.0
64-65	33.1508404691849	37.0	33.0	37.0	22.0	40.0
66-67	32.92863667326774	37.0	33.0	37.0	22.0	40.0
68-69	32.713700610168985	37.0	33.0	37.0	22.0	40.0
70-71	32.45151659571431	37.0	33.0	37.0	22.0	38.5
72-73	32.188796485784096	35.0	33.0	37.0	22.0	37.0
74-75	31.94178426702842	33.0	33.0	37.0	22.0	37.0
76-77	31.712632335764688	33.0	33.0	37.0	22.0	37.0
78-79	31.49245335827932	33.0	30.0	37.0	22.0	37.0
80-81	31.324566119143093	33.0	27.0	37.0	22.0	37.0
82-83	31.150221654790343	33.0	27.0	37.0	15.0	37.0
84-85	30.95627324180412	33.0	27.0	37.0	15.0	37.0
86-87	30.729198748503734	33.0	27.0	37.0	15.0	37.0
88-89	30.55631022415993	33.0	27.0	37.0	15.0	37.0
90-91	30.428081539023022	33.0	27.0	37.0	15.0	37.0
92-93	30.297303109390022	33.0	27.0	37.0	15.0	37.0
94-95	30.138906134588794	33.0	27.0	37.0	15.0	37.0
96-97	30.007627003458744	33.0	27.0	37.0	15.0	37.0
98-99	29.85430808543526	33.0	27.0	37.0	15.0	37.0
100	29.741187261997254	33.0	27.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	9.0
7	200.0
8	1128.0
9	2534.0
10	4824.0
11	10783.0
12	23964.0
13	43352.0
14	59285.0
15	66129.0
16	73360.0
17	82612.0
18	95134.0
19	109485.0
20	125245.0
21	145634.0
22	162036.0
23	161420.0
24	157306.0
25	169675.0
26	194385.0
27	230815.0
28	282342.0
29	344541.0
30	427767.0
31	530319.0
32	673170.0
33	875616.0
34	1134510.0
35	1497623.0
36	2118004.0
37	2835739.0
38	1147552.0
39	1158.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.835657615097084	19.161232570789345	13.170022135003201	24.833087679110374
2	15.166966378929352	21.380608070090013	38.37520744567093	25.07721810530971
3	17.78482519555351	27.05475730442944	29.795001972474154	25.36541552754289
4	12.4665405646097	18.20868676513112	37.070377512531735	32.25439515772744
5	13.414450850363648	38.002674401549335	33.774591212195645	14.80828353589137
6	30.422306147508692	37.17085728040575	17.97431671495619	14.432519857129364
7	26.321876880784036	32.75576014260978	22.607142012283447	18.315220964322737
8	26.594662548332447	33.60912362284979	20.404018219160875	19.392195609656888
9	26.953417137704506	14.901586692322516	20.01311514981132	38.131881020161664
10-11	24.48231377364875	26.101653167427042	27.3678870784855	22.048145980438708
12-13	26.08923442103527	23.941048458155212	26.884756101607593	23.08496101920193
14-15	23.915748542177734	24.410169295816615	25.256097148791362	26.41798501321429
16-17	22.82621148583346	27.028429181711516	26.35921883099407	23.786140501460956
18-19	23.27332455787105	26.321342216337097	28.34950976751311	22.055823458278738
20-21	24.420020943824145	25.623703296646706	27.58256812890208	22.373707630627074
22-23	23.876444117680663	25.731639441154137	26.694558059139204	23.697358382025993
24-25	23.350507098479113	26.223909168950776	26.89178090350928	23.533802829060836
26-27	23.61910992200467	25.636867052447514	27.151208758553437	23.592814266994377
28-29	23.223669953579726	25.704598827816145	27.69111751258701	23.38061370601712
30-31	23.808070535336455	25.705944003376075	27.452388270580496	23.033597190706974
32-33	23.037693753476688	26.079626375850175	27.208455592932467	23.674224277740674
34-35	23.09002260595348	26.22300762585387	27.591428647932066	23.09554112026058
36-37	23.691027460337217	25.805515911388905	27.152027607892492	23.351429020381385
38-39	23.758767459810397	25.531174609569916	27.224219974380137	23.485837956239553
40-41	23.350061079956326	25.76552771210028	27.363751495069266	23.520659712874135
42-43	23.912761475706635	25.6550202693418	26.92125133653632	23.510966918415253
44-45	23.673988629922086	25.896743285551487	26.76723924908456	23.662028835441866
46-47	23.222100147279786	25.943341606988056	27.169642604750834	23.664915640981324
48-49	23.76247788089754	25.993056662097846	26.70147032722609	23.542995129778525
50-51	22.93402834775123	26.413437928916046	26.29246217829993	24.360071545032795
52-53	22.680102777705553	26.54289766678739	25.74944313270009	25.027556422806967
54-55	22.980091757438682	26.359175917937026	25.681189028795032	24.979543295829252
56-57	23.390901252540676	25.61349804491786	25.78342903246208	25.212171670079382
58-59	22.90562297173646	25.62039189257405	26.56319536837879	24.9107897673107
60-61	23.106824684340836	25.85002120737564	26.115581212644123	24.927572895639404
62-63	22.599445986594084	25.759121121575244	26.146364778861543	25.495068112969125
64-65	22.263022067455516	25.70911731483444	26.34005950549601	25.687801112214032
66-67	23.215820865320072	25.408303031379976	26.02175575688761	25.35412034641234
68-69	23.133616217219082	25.15664168126562	26.078834493634023	25.630907607881277
70-71	22.54928014216778	25.261271806013685	26.267846311478827	25.921601740339707
72-73	23.19568694750547	25.116389600204503	26.342611367800423	25.345312084489606
74-75	23.26332708124035	25.109794820357163	26.285437557721497	25.341440540680992
76-77	22.745690897382797	25.328855120974435	26.60710827001668	25.31834571162609
78-79	22.88341832723416	25.364967765369258	26.45891368337011	25.292700224026476
80-81	22.462149833466956	25.439364086159983	26.592689372680805	25.50579670769226
82-83	22.63848977418786	25.276571211441034	26.263350414703147	25.821588599667955
84-85	22.81064451872736	25.36902554250479	25.921419706273667	25.898910232494178
86-87	22.662273599426946	24.970117162482417	26.25089775442081	26.116711483669825
88-89	22.92540015459849	24.463018666960657	26.432272679386198	26.17930849905466
90-91	23.058543097965288	24.724288738528365	26.195150139343426	26.022018024162918
92-93	22.78812939425908	24.76031511917062	26.258222774587814	26.193332711982485
94-95	22.243199671001396	24.82130918742748	26.60285186522299	26.33263927634813
96-97	22.87155075407912	24.69332188905078	26.09904949667919	26.33607786019091
98-99	22.79530484446535	24.800214577324773	25.954608525669766	26.449872052540112
100	22.65734124730494	24.873916227892092	26.048123260441415	26.420619264361555
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	112.0
1	117.5
2	171.0
3	234.5
4	235.5
5	260.5
6	350.0
7	603.5
8	994.5
9	1210.0
10	1119.5
11	1069.0
12	1244.0
13	1488.5
14	1870.0
15	2384.5
16	2982.5
17	3657.5
18	4660.5
19	6058.0
20	7650.5
21	9925.5
22	13217.0
23	17498.0
24	23227.5
25	30452.5
26	40239.5
27	51923.0
28	64476.0
29	82447.5
30	102457.5
31	121806.5
32	144722.0
33	168028.0
34	192512.0
35	215104.0
36	237021.0
37	266808.0
38	289109.0
39	304666.0
40	318296.5
41	330796.0
42	349448.5
43	380788.0
44	424793.5
45	475338.5
46	532829.5
47	587280.0
48	649842.0
49	702797.5
50	762847.5
51	756097.5
52	650903.5
53	579393.0
54	542454.0
55	497885.0
56	452055.0
57	399034.5
58	352528.0
59	314921.0
60	266034.5
61	216489.5
62	173884.0
63	137994.5
64	106787.5
65	81604.0
66	62904.5
67	48374.0
68	38044.0
69	31362.0
70	27159.0
71	23512.5
72	19887.0
73	18594.5
74	15693.0
75	12254.5
76	9116.0
77	6364.5
78	4885.5
79	3584.0
80	2542.5
81	1883.5
82	1439.5
83	1025.5
84	675.5
85	431.0
86	290.5
87	164.5
88	90.0
89	58.5
90	41.5
91	28.5
92	21.5
93	17.5
94	10.5
95	6.5
96	4.0
97	2.0
98	1.5
99	2.0
100	2.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04908738657245292
2	0.024108521419449396
3	0.021308915743183613
4	0.022150248018952604
5	0.015535635643941219
6	0.02493534796632582
7	0.049566075625907695
8	0.006179440508234322
9	0.0039673168521175755
10-11	0.001990911290505072
12-13	0.0011785904725212174
14-15	0.0022483879783481687
16-17	0.006959123436209897
18-19	0.005280085316895054
20-21	0.009087839151194372
22-23	0.01913668284152143
24-25	0.02645119663559926
26-27	0.029083986429600507
28-29	0.045587879477120695
30-31	0.04328509501542539
32-33	0.04117813789377977
34-35	0.049123650894684345
36-37	0.046269648735071424
38-39	0.05602112498310082
40-41	0.053366576595760724
42-43	0.05875908131157319
44-45	0.059147109559449414
46-47	0.06367652340615403
48-49	0.06617876164012215
50-51	0.034295169534255855
52-53	2.5747668784309674E-4
54-55	0.0
56-57	0.0
58-59	0.0
60-61	0.0
62-63	6.056141812647487E-4
64-65	1.5956301781825714E-4
66-67	2.9736744229766105E-4
68-69	0.0010008952935872495
70-71	2.1758593338853246E-5
72-73	1.414308567025461E-4
74-75	4.71436189008487E-5
76-77	8.340794113227077E-5
78-79	0.0
80-81	1.4505728892568831E-5
82-83	1.6318945004139936E-4
84-85	9.283666491244052E-4
86-87	0.0034668692053239506
88-89	0.0023825659706044307
90-91	0.0036046736298033545
92-93	0.0035611564431256484
94-95	0.002636416226224385
96-97	0.002005417019397641
98-99	0.003999954742125856
100	0.00285762859183606
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.3787656E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.70659976092569
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.84760256757416	33.46181811053372
2	13.43602608105742	12.01358080766386
3	5.03114135798196	6.74775669095819
4	2.3038325582626817	4.119860803937569
5	1.2059723760738323	2.6957462169932693
6	0.7449811123985436	1.998334345287053
7	0.4799877858723597	1.5021035283189939
8	0.3320960920965818	1.1877509657223522
9	0.24034074729985735	0.9670335836198856
>10	1.1691413714705052	9.401859846991638
>50	0.09752665712707884	3.0683318810546436
>100	0.08817654988347189	8.274799129732225
>500	0.013691403911142116	4.262067449846216
>1k	0.008863410069613725	6.90128044650552
>5k	4.021160568383214E-4	1.168842796928833
>10k+	2.1781286412075743E-4	2.228833395906013
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	59134	0.42889088616658266	No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	56718	0.41136796566435946	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	21272	0.1542829325013621	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	17892	0.12976825067292078	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	17879	0.12967396343511906	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17499	0.12691787494553097	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	17481	0.12678732338549786	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	16977	0.12313187970457051	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	16328	0.11842477067893194	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	16160	0.11720628945195614	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	15777	0.11442844236902923	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.4505728892568831E-5	0.0
2	4.351718667770649E-5	7.2528644462844155E-6	0.0	1.4505728892568831E-5	0.0
3	4.351718667770649E-5	7.2528644462844155E-6	0.0	1.4505728892568831E-5	0.0
4	4.351718667770649E-5	1.4505728892568831E-5	0.0	1.4505728892568831E-5	0.0
5	7.252864446284415E-5	1.4505728892568831E-5	0.0	4.351718667770649E-5	0.0
6	1.0879296669426623E-4	1.4505728892568831E-5	0.0	5.077005112399091E-5	0.0
7	1.450572889256883E-4	1.4505728892568831E-5	0.0	5.8022915570275324E-5	7.2528644462844155E-6
8	1.5231015337197274E-4	1.4505728892568831E-5	0.0	6.527578001655974E-5	1.4505728892568831E-5
9	1.5231015337197274E-4	4.351718667770649E-5	0.0	1.0879296669426623E-4	1.4505728892568831E-5
10-11	1.7044231448768376E-4	4.351718667770649E-5	0.0	1.7406874671082597E-4	1.4505728892568831E-5
12-13	2.5022382339681237E-4	4.71436189008487E-5	0.0	2.0670663671910585E-4	1.4505728892568831E-5
14-15	3.191260356365143E-4	5.077005112399091E-5	0.0	2.2846523005795908E-4	3.263789000827987E-5
16-17	3.5539035786793637E-4	5.077005112399091E-5	0.0	2.683559845125234E-4	5.077005112399091E-5
18-19	4.859419179010558E-4	6.164934779341754E-5	0.0	3.4813749342165194E-4	5.8022915570275324E-5
20-21	5.802291557027532E-4	7.252864446284415E-5	0.0	5.149533756861935E-4	6.164934779341754E-5
22-23	6.237463423804598E-4	7.978150890912857E-5	0.0	0.001479584347042021	7.615507668598637E-5
24-25	8.086943857607123E-4	7.978150890912857E-5	0.0	0.00451853455003519	8.703437335541299E-5
26-27	8.413322757689923E-4	7.978150890912857E-5	0.0	0.0077895764153094625	8.703437335541299E-5
28-29	9.827631324715383E-4	7.978150890912857E-5	0.0	0.021243639963167055	8.703437335541299E-5
30-31	0.0010951825313889468	7.978150890912857E-5	0.0	0.07019322211114057	9.42872378016974E-5
32-33	0.0011169411247278	7.978150890912857E-5	0.0	0.17541777949783488	9.42872378016974E-5
34-35	0.0012257340914220661	8.340794113227077E-5	0.0	0.3174760089749846	9.79136700248396E-5
36-37	0.001410682134802319	8.703437335541299E-5	0.0	0.5366503196772533	1.0154010224798182E-4
38-39	0.0015847508815131448	9.42872378016974E-5	0.0	0.876900323013571	1.0154010224798182E-4
40-41	0.001624641635967709	1.0154010224798182E-4	0.0	1.3036588670329459	1.0154010224798182E-4
42-43	0.0017298081704388332	1.0154010224798182E-4	0.0	1.6751941011583114	1.0154010224798182E-4
44-45	0.0018929976204802325	1.0154010224798182E-4	0.0	2.104618072861696	1.1967226336369286E-4
46-47	0.0021178364183150494	1.0154010224798182E-4	0.0	2.5709156074099906	1.2329869558683508E-4
48-49	0.0022411351139018845	1.0154010224798182E-4	0.0	3.054464080043773	1.2329869558683508E-4
50-51	0.0022483879783481687	1.0154010224798182E-4	0.0	3.5823021694187904	1.2329869558683508E-4
52-53	0.0022628937072407375	1.0154010224798182E-4	0.0	4.10695625130189	1.341779922562617E-4
54-55	0.002306410893918444	1.0154010224798182E-4	0.0	4.61918254995628	1.4868372114883052E-4
56-57	0.0024768532084061276	1.1604583114055065E-4	0.0	5.188314823056218	1.5231015337197274E-4
58-59	0.0025022382339681235	1.1604583114055065E-4	0.0	5.797374114932952	1.5593658559511495E-4
60-61	0.002527623259530119	1.1967226336369286E-4	0.0	6.40147607396065	1.5956301781825714E-4
62-63	0.0025385025561995454	1.2329869558683508E-4	0.0	7.004131086531315	1.5956301781825714E-4
64-65	0.002582019742877252	1.378044244794039E-4	0.0	7.644740338749386	1.5956301781825714E-4
66-67	0.002766967786257505	1.378044244794039E-4	0.0	8.325987390459987	1.6681588226454157E-4
68-69	0.002828617134050922	1.378044244794039E-4	0.0	9.06389019279274	1.6681588226454157E-4
70-71	0.0029120250751831925	1.414308567025461E-4	0.0	9.881219113676757	1.6681588226454157E-4
72-73	0.0029990594485386057	1.4868372114883052E-4	0.0	10.648550413500308	1.813216111571104E-4
74-75	0.003046203067439454	1.5593658559511495E-4	0.0	11.397038771492413	1.813216111571104E-4
76-77	0.003067961660778308	1.5956301781825714E-4	0.0	12.189526631647903	1.813216111571104E-4
78-79	0.003089720254117161	1.5956301781825714E-4	0.0	12.991269146836853	1.813216111571104E-4
80-81	0.0034124727219768175	1.5956301781825714E-4	0.0	13.838360196976193	2.0308020449596363E-4
82-83	0.0034850013664396618	1.7406874671082597E-4	0.0	14.735800632101643	2.248387978348169E-4
84-85	0.0035647828753487903	1.885744756033948E-4	0.0	15.599029305633966	2.248387978348169E-4
86-87	0.0036590701131504876	1.885744756033948E-4	0.0	16.467262455634227	2.320916622811013E-4
88	0.003677202274266199	1.9582734004967923E-4	0.0	17.141136970635184	2.3934452672738572E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACATA	4685	0.0	20.766241	1
GTACTAG	5460	0.0	20.401066	1
GTATAAG	6060	0.0	19.854753	1
TACATGA	6315	0.0	19.125292	2
GTACAAG	6720	0.0	18.674076	1
TAGTACT	6420	0.0	18.44529	4
CATGGGG	32800	0.0	17.578857	4
GTACAAA	7390	0.0	17.108227	1
TAGGCAT	7815	0.0	16.534842	5
ACGTTAA	1760	0.0	16.282774	8
GTACATG	152700	0.0	16.162186	1
GGTCGCC	9185	0.0	15.958243	94
TACATGG	150020	0.0	15.640863	2
TCTAACG	3000	0.0	15.50823	2
CTGTGCG	7200	0.0	15.3986025	9
GTGATCG	3910	0.0	15.37958	8
CTAGGCA	8970	0.0	15.13998	4
CTAGTAC	7220	0.0	15.100223	3
CGTTAAC	1935	0.0	15.052634	9
GTATATA	4310	0.0	14.830602	1
>>END_MODULE