FastQCFastQC Report
Fri 27 May 2016
ERR765468_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765468_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11335232
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA346890.3060281430499173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT330750.2917893519956186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151630.13376876626786288No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA137440.12125027524800551No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG136410.12034160394776217No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG133350.11764205620140814No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA129910.11460727049962453No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA120960.10671153444411195No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG120090.10594401596720737No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT117220.10341208719856815No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA115030.1014800579291187No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG38000.032.7499241
GTATAAG50150.020.7888321
GTGATCG37500.020.537128
CATGGGG350900.020.1811054
TACAAGG52750.020.1193032
ATTGCGT36800.019.6516886
GTACATA24900.019.2375091
GTATCAA734050.018.9755121
TAGTACT55200.018.7158934
TTGCGTG40300.018.2945447
GTACTAG46850.018.1433321
GTACATG1078650.018.1074121
TACATGG1064100.018.0893272
TGATCGC42900.018.061659
ATGGGAG90750.017.8007685
CTATTGA48750.017.7244999
ATGGGGG166550.017.5940935
ACGCAAG15350.017.437878
CGTTAAC14350.017.3449081
GGGCTAT56950.017.3161986
Produced by FastQC (version 0.11.5)