FastQCFastQC Report
Fri 27 May 2016
ERR765465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7985433
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA444920.5571645269580248No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT432740.5419117535642713No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA250130.3132328578800924No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC240200.3007977150393723No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG228880.28662190265700055No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193570.24240388717806535No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG174220.2181722644219794No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG173380.2171203490155136No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC153960.19280106664222216No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC140620.17609564816334944No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT136180.17053552387203047No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT134340.16823132821977219No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT127010.15905211401811273No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA109770.13746280258064902No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC108510.1358849294709504No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94400.11821525520281743No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG92830.1162491752169231No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG80390.1006708089592637No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACAC73050.024.4983023
TACACTC73000.023.9765075
ATCTACA69850.023.1909312
AGTACTT252250.019.8549212-13
CTACACT98400.019.520964
GAGTACT238450.019.5156812-13
CATGGGG156000.019.495934
ATTGCGT13400.018.598616
CATGGGT72200.018.1929474
CACTCTT94450.017.8750927
GTATAAG25950.017.425081
TATTCGC4600.017.3778119
GTACTTT271550.017.16189214-15
TACTTTT295100.017.05792814-15
ACACTCT106500.016.3807496
GTATTAC11000.016.2716581
GTATTAG35700.016.0965391
GTATAAA46050.015.854151
ACTCTTT123150.015.8077448
ACTTTTT303450.015.74563816-17
Produced by FastQC (version 0.11.5)