FastQCFastQC Report
Fri 27 May 2016
ERR765465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7985433
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA452440.5665816744063847No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT424860.532043785227426No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA260410.3261062988068399No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG251400.31482325379224896No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC240220.30082276064428815No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG201040.2517584206141358No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT190870.23902273051442544No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG190200.23818370274974443No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT159380.19958842557441783No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC151700.18997091328673096No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC149320.1869904863017447No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT135710.16994695215650799No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132900.16642804466583092No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC123440.1545814735406333No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA115320.14441295794479775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT104270.13057526122878998No Hit
GCTTCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTG89970.1126676537139564No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC85610.10720771184230084No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG80690.10104649303300146No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAC30450.019.3083481
AGTACTT237750.019.17239612-13
GAGTACT222300.018.7080712-13
TCTACAC44150.017.3548473
TACTTTT273100.016.9577514-15
GTACTTT251000.016.7096714-15
TACACTC39200.016.6625045
CATGGGG173550.016.1655774
TAGTACT24850.016.0743734
CGTTAAC8250.015.963441
GTATTAG33450.015.7486851
TCCGATC18550.015.7112843
GTATAAG23600.015.5455091
ATCTACA42100.015.4176932
ATTATAC19200.015.179393
ACTTTTT284600.014.93674516-17
TCTCGCG8500.014.9269138
TATACCG11800.014.734365
CATGGGT76250.014.7298924
CTAGTAC30450.014.6656133
Produced by FastQC (version 0.11.5)