FastQCFastQC Report
Fri 27 May 2016
ERR765464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11505712
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA228410.19851878788553026No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT228160.1983015045048929No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG168740.14665759059500186No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT159880.13895706758521334No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG121330.10545197029093029No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTAAC15350.023.8799531
GTATAAG46500.020.4148241
TAGTACT50850.019.9653994
GTACTAG44550.019.620611
TATTCGC8000.018.8064759
CATGGGG249000.018.2344654
ATTGCGT29350.017.4601066
TCTACAC87300.017.2799223
CTAGTAC60400.017.117363
GTGATCG37450.017.0739698
TAGGCAT65400.017.0361235
GATCTAC72950.016.9425071
ATAAGGT42900.016.8699763
CTACACT111250.016.7728044
GTATCAA590600.016.6382581
TACACTC79400.016.5782135
CTAACGC25850.016.3618723
CTATTGA55800.016.2618719
TGATCGC39000.016.154289
CTGTGCG66650.016.1540999
Produced by FastQC (version 0.11.5)