##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765464_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	11505712
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.159033269735936	33.0	33.0	33.0	33.0	33.0
2	32.15092694828447	33.0	33.0	33.0	33.0	33.0
3	32.14285052502618	33.0	33.0	33.0	33.0	33.0
4	35.913308363706655	37.0	37.0	37.0	33.0	37.0
5	35.919644173259336	37.0	37.0	37.0	33.0	37.0
6	35.901004388081326	37.0	37.0	37.0	33.0	37.0
7	35.83684842798082	37.0	37.0	37.0	33.0	37.0
8	35.82838784770556	37.0	37.0	37.0	33.0	37.0
9	35.83849560983275	37.0	37.0	37.0	33.0	37.0
10-11	35.80329909179024	37.0	37.0	37.0	33.0	37.0
12-13	35.77709136992131	37.0	37.0	37.0	33.0	37.0
14-15	37.47024165040807	40.0	37.0	40.0	33.0	40.0
16-17	37.505615341319164	40.0	37.0	40.0	33.0	40.0
18-19	37.53729152094195	40.0	37.0	40.0	33.0	40.0
20-21	37.53973648045423	40.0	37.0	40.0	33.0	40.0
22-23	37.51458953604957	40.0	37.0	40.0	33.0	40.0
24-25	37.482509122425455	40.0	37.0	40.0	33.0	40.0
26-27	37.46323834631008	40.0	37.0	40.0	33.0	40.0
28-29	37.438935330555815	40.0	37.0	40.0	33.0	40.0
30-31	37.363066753278716	40.0	37.0	40.0	33.0	40.0
32-33	37.26847182512478	40.0	37.0	40.0	33.0	40.0
34-35	37.160512143881235	40.0	37.0	40.0	33.0	40.0
36-37	37.0998880816763	40.0	37.0	40.0	33.0	40.0
38-39	37.00537524318356	37.0	37.0	40.0	33.0	40.0
40-41	36.866646757714776	37.0	37.0	40.0	33.0	40.0
42-43	36.760688647516986	37.0	37.0	40.0	33.0	40.0
44-45	36.62703724897686	37.0	37.0	40.0	33.0	40.0
46-47	36.429653679841806	37.0	37.0	40.0	33.0	40.0
48-49	36.20834877493891	37.0	37.0	40.0	33.0	40.0
50-51	35.99374645393523	37.0	37.0	40.0	33.0	40.0
52-53	35.820511629354186	37.0	37.0	40.0	27.0	40.0
54-55	35.69348937293059	37.0	37.0	40.0	27.0	40.0
56-57	35.53172680665047	37.0	33.0	40.0	27.0	40.0
58-59	35.353213603816954	37.0	33.0	40.0	27.0	40.0
60-61	35.08576101157408	37.0	33.0	38.5	27.0	40.0
62-63	34.87436635820538	37.0	33.0	37.0	27.0	40.0
64-65	34.638184451340344	37.0	33.0	37.0	27.0	40.0
66-67	34.403120554382035	37.0	33.0	37.0	27.0	40.0
68-69	34.17060782505246	37.0	33.0	37.0	27.0	40.0
70-71	33.96244982492175	37.0	33.0	37.0	27.0	38.5
72-73	33.71956755044799	37.0	33.0	37.0	27.0	37.0
74-75	33.54141929678059	37.0	33.0	37.0	27.0	37.0
76-77	33.34126175763829	37.0	33.0	37.0	27.0	37.0
78-79	33.149752097045365	37.0	33.0	37.0	27.0	37.0
80-81	32.98462328971905	37.0	33.0	37.0	27.0	37.0
82-83	32.826449245383515	33.0	33.0	37.0	27.0	37.0
84-85	32.69070258320389	33.0	33.0	37.0	27.0	37.0
86-87	32.56557182206542	33.0	33.0	37.0	27.0	37.0
88-89	32.45911113540822	33.0	33.0	37.0	27.0	37.0
90-91	32.351491198458646	33.0	33.0	37.0	24.5	37.0
92-93	32.20778088309528	33.0	33.0	37.0	22.0	37.0
94-95	32.130259257314975	33.0	33.0	37.0	22.0	37.0
96-97	31.975497605015665	33.0	33.0	37.0	22.0	37.0
98-99	31.87056663681483	33.0	33.0	37.0	22.0	37.0
100	31.791661828490057	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	4.0
7	227.0
8	1362.0
9	1766.0
10	1762.0
11	3241.0
12	7438.0
13	15056.0
14	19564.0
15	20714.0
16	22002.0
17	26070.0
18	32138.0
19	38770.0
20	47883.0
21	62094.0
22	72792.0
23	73023.0
24	73339.0
25	76476.0
26	83188.0
27	97687.0
28	122067.0
29	157093.0
30	207924.0
31	279054.0
32	384316.0
33	542286.0
34	792402.0
35	1276415.0
36	2368509.0
37	3524390.0
38	1074163.0
39	497.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.36152056484461	18.809671987766187	12.749583833158063	25.07922361423114
2	16.20206530442241	20.820717224278695	37.32867210910749	25.64854536219141
3	18.451418716075814	25.357571315648613	29.2922682542384	26.898741714037172
4	13.245578695162585	17.090389624161382	36.336817529719106	33.32721415095693
5	14.357819636414842	37.07387352618671	33.37859350981064	15.189713327587803
6	32.08412595407838	36.77116773265936	16.96020071825651	14.184505595005756
7	27.435959394375047	31.92189180003842	21.727520923857067	18.91462788172947
8	26.88184866078553	32.922985910929036	20.082645475750827	20.112519952534612
9	26.998326714856514	14.483855723202774	19.567423065259163	38.950394496681554
10-11	25.381866344768916	25.46750772161206	26.960793923643898	22.18983200997512
12-13	26.40093295478557	23.326499529945085	26.662126736472224	23.610440778797116
14-15	24.17698666271105	23.884235880168365	24.571360100282288	27.367417356838292
16-17	23.730028179610542	26.576684693568954	25.332919263458	24.3603678633625
18-19	24.0469512697581	26.019027856829684	27.060890463089066	22.87313041032315
20-21	25.261704498026777	25.242665833676213	26.04421055120084	23.451419117096172
22-23	24.898367818541516	25.025665526304948	25.73301021249804	24.3429564426555
24-25	24.450995218177574	25.425807656382908	25.826712749030527	24.29648437640899
26-27	24.554407943654947	25.4483646349344	25.642492939267925	24.354734482142725
28-29	24.2051892278919	25.285681922548132	26.329666259024044	24.179462590535927
30-31	24.754919071109743	25.117449237039637	26.068670909928503	24.058960781922114
32-33	24.248307138246044	25.542222572266642	25.720588749213054	24.488881540274264
34-35	24.43783522929362	25.395204198034698	26.09685842758665	24.07010214508503
36-37	24.588953386417217	25.327744046533834	25.676834715407704	24.40646785164125
38-39	24.703485647293768	24.86519783384766	25.96976029916711	24.46155621969146
40-41	24.473373632837678	24.861094317477818	26.220752746711607	24.444779302972897
42-43	24.823020387161375	25.02188560382711	25.783548944484618	24.371545064526895
44-45	24.571772810803523	25.194717070410178	25.832441496658536	24.401068622127763
46-47	24.319525266772537	25.124850821169726	25.89957893312194	24.65604497893579
48-49	24.890651737939006	25.102730841175685	25.677335948485908	24.3292814723994
50-51	24.260583953406666	25.401066613633834	25.352087150935493	24.986262282024008
52-53	24.09252596802004	25.517016495054808	25.080821826172293	25.309635710752858
54-55	24.337279942218156	25.305363133673847	25.007716750044374	25.349640174063627
56-57	24.53092425295344	24.923158820403412	25.092885479684586	25.453031446958562
58-59	24.175295668649106	24.83600137233054	25.820936246171826	25.167766712848525
60-61	24.276482983400946	25.16986828669705	25.334935284184386	25.218713445717615
62-63	24.08061538121856	25.05629692596683	25.24920878322014	25.61387890959447
64-65	23.719030720315505	25.065136609342975	25.382129704406047	25.83370296593548
66-67	24.442599823587486	24.96219700776766	25.141530317282612	25.453672851362242
68-69	24.289661431129495	24.64257572939975	25.356144398905546	25.711618440565214
70-71	23.930353369998787	24.81858492766865	25.338636543003336	25.912425159329228
72-73	24.57203971199231	24.814953681757753	25.3034371075036	25.309569498746338
74-75	24.499749942331157	24.909233498931712	25.29223371465752	25.298782844079614
76-77	24.11562000291277	24.939470500906406	25.39400510990707	25.550904386273753
78-79	24.14303342619534	25.031140751241427	25.356543838351005	25.469281984212227
80-81	23.962195008849722	24.942491809781984	25.537478303777622	25.557834877590675
82-83	24.152820463880712	24.75753989330843	25.550078175648327	25.539561467162535
84-85	24.305530173482133	24.815403091398196	25.106565956200882	25.772500778918793
86-87	23.87574891259265	24.744892549099777	25.481832448701248	25.897526089606327
88-89	24.10927163714593	24.204651411001823	25.59842681605732	26.08765013579493
90-91	24.193213767084305	24.439276810537628	25.44016437925641	25.927345043121658
92-93	24.12889892720102	24.42486969715959	25.466505853578447	25.979725522060942
94-95	23.456739208176867	24.497099653463792	25.850303536967374	26.19585760139197
96-97	23.84545405478554	24.486110144192924	25.46228901736515	26.20614678365638
98-99	23.66853853260006	24.591822131696865	25.344882501470096	26.39475683423298
100	23.748519006907376	24.52167323818645	25.36417154043881	26.365636214467358
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	699.0
1	651.5
2	661.5
3	690.5
4	540.5
5	436.5
6	598.0
7	1125.5
8	1797.0
9	1830.0
10	1292.5
11	1055.0
12	1194.5
13	1446.5
14	1776.5
15	2184.0
16	2698.5
17	3381.5
18	4164.0
19	5060.0
20	6106.5
21	7393.0
22	9223.0
23	11309.5
24	13665.0
25	17434.0
26	23003.0
27	29611.0
28	37287.0
29	49682.0
30	63479.0
31	75389.0
32	91893.0
33	110737.5
34	130629.5
35	147524.5
36	164855.0
37	191563.5
38	208476.0
39	217720.0
40	225677.5
41	232994.0
42	248787.0
43	277425.5
44	315546.5
45	356090.5
46	403001.0
47	449254.5
48	495163.5
49	529493.0
50	553679.5
51	561695.0
52	551712.0
53	546951.0
54	529821.5
55	492681.5
56	457127.0
57	414497.5
58	377372.0
59	346912.0
60	298474.5
61	245683.0
62	200341.5
63	160643.5
64	123073.5
65	93839.0
66	73635.0
67	57139.0
68	44904.0
69	36382.0
70	30415.0
71	25516.0
72	21220.5
73	19941.0
74	16503.0
75	12890.5
76	10418.5
77	7732.5
78	5900.0
79	4271.0
80	3045.5
81	2233.5
82	1728.0
83	1318.5
84	935.0
85	614.0
86	420.5
87	255.0
88	139.5
89	86.0
90	62.0
91	46.5
92	29.0
93	26.0
94	21.0
95	10.0
96	8.0
97	6.0
98	3.5
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.02184132542166882
2	0.005040974430787074
3	0.008813013918651883
4	0.007883041049523924
5	0.009708221446877864
6	0.005475541192061822
7	0.00783958437339645
8	0.007891732384749418
9	0.006944376845170469
10-11	0.026895336855294136
12-13	0.028963874638961933
14-15	0.012059227625374248
16-17	0.003541719104389194
18-19	0.01143779715675136
20-21	0.0015470576701381018
22-23	5.214801135296972E-5
24-25	0.00322013970104588
26-27	0.0015383663349126068
28-29	0.0011081452412506066
30-31	0.026795386500200944
32-33	0.02094611789344284
34-35	0.007822201702945458
36-37	0.008382792824989884
38-39	0.006118699998748448
40-41	0.014627517184508007
42-43	0.00375465681741382
44-45	0.003728582811737335
46-47	0.01288490447179627
48-49	0.01725664609022023
50-51	0.007735288350690509
52-53	0.021524091685938253
54-55	0.027260372934764923
56-57	0.014136456744267542
58-59	0.01038614559446647
60-61	0.008600076205627256
62-63	0.015240256317905404
64-65	0.009082445310642227
66-67	0.017321831104411444
68-69	0.005671096234635457
70-71	0.0052495664761989524
72-73	0.010668613989295056
74-75	0.0031810286925311538
76-77	0.010407873932530207
78-79	0.002516141547780789
80-81	0.0072051169019353165
82-83	0.0018338717325794354
84-85	0.008539236859048793
86-87	0.003163646022080163
88-89	0.0011124909088633542
90-91	0.0010777255679613744
92-93	1.303700283824243E-4
94-95	0.0024031541898493548
96-97	7.387634941670711E-4
98-99	0.004567296660997599
100	0.00663148877705265
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.1505712E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.746860892921
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.42462771056009	25.88953091990983
2	14.198934650216376	10.151346835379215
3	5.208716972873006	5.5858584317966375
4	2.4445850083552703	3.4954496093838383
5	1.368619165781784	2.4461919467293516
6	0.8368576968950237	1.7949021406845993
7	0.570532010550679	1.4276309891279562
8	0.3939720733762745	1.126661192214188
9	0.313201691574839	1.0076379570137985
>10	1.8351949033758395	12.524975031191069
>50	0.19965861628603526	4.988120057878156
>100	0.16630372652504505	12.131885728116226
>500	0.022999653577664196	5.752059141145368
>1k	0.015064818592992087	9.384684468535347
>5k	6.094178826510806E-4	1.5031321352772564
>10k+	1.2188357653021612E-4	0.7899334156173048
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	22841	0.19851878788553026	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	22816	0.1983015045048929	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	16874	0.14665759059500186	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15988	0.13895706758521334	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	12133	0.10545197029093029	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.9111008514727293E-4	0.0	0.0	0.0	0.0
2	8.343681816475157E-4	1.738267045098991E-5	0.0	8.691335225494955E-6	8.691335225494955E-6
3	8.691335225494954E-4	1.738267045098991E-5	0.0	2.6074005676484864E-5	8.691335225494955E-6
4	9.125901986769702E-4	2.6074005676484864E-5	0.0	3.476534090197982E-5	8.691335225494955E-6
5	0.0027899186073838803	2.6074005676484864E-5	0.0	1.2167869315692935E-4	8.691335225494955E-6
6	0.006266452697581862	2.6074005676484864E-5	0.0	2.259747158628688E-4	1.738267045098991E-5
7	0.007074746873552893	2.6074005676484864E-5	0.0	2.433573863138587E-4	1.738267045098991E-5
8	0.008413212498279116	2.6074005676484864E-5	0.0	2.5204872153935365E-4	2.6074005676484864E-5
9	0.009360568037858065	1.0429602270593946E-4	0.0	3.041967328923234E-4	6.953068180395964E-5
10-11	0.017391361786215403	1.0429602270593946E-4	0.0	4.519494317257376E-4	8.256768464220206E-5
12-13	0.034578477194631675	1.0429602270593946E-4	0.0	9.038988634514752E-4	9.56046874804445E-5
14-15	0.05549417541478528	1.5644403405890917E-4	0.0	0.0015036009940106272	1.347156959951718E-4
16-17	0.06467222541290796	1.5644403405890917E-4	0.0	0.001786069388839213	1.5644403405890917E-4
18-19	0.06996090289762164	1.7382670450989907E-4	0.0	0.0018990567467706476	1.7382670450989907E-4
20-21	0.09124163719724603	1.91209374960889E-4	0.0	0.0024379195307513346	1.8251803973539404E-4
22-23	0.09724300417045029	2.2162904825012133E-4	0.0	0.0032462137067223655	1.955550425736365E-4
24-25	0.12519868392325484	2.433573863138587E-4	0.0	0.006110008663522953	2.085920454118789E-4
26-27	0.19872303426332938	2.433573863138587E-4	0.0	0.014918676914562088	2.085920454118789E-4
28-29	0.2126465532945723	2.433573863138587E-4	0.0	0.028611875562329386	2.259747158628688E-4
30-31	0.21690965322267758	2.9115973005408097E-4	0.0	0.07190776198813251	2.3032038347561628E-4
32-33	0.2206947297133806	2.9550539766682845E-4	0.0	0.1513291832787054	2.433573863138587E-4
34-35	0.22333254995431834	3.82418749921778E-4	0.0	0.2596101831855343	2.477030539266062E-4
36-37	0.22780858759544825	4.693321021767275E-4	0.0	0.43178553400258934	2.563943891521011E-4
38-39	0.23184136714007791	4.7802343740222246E-4	0.0	0.6939640067472573	2.694313919903436E-4
40-41	0.2355308389433005	5.562454544316771E-4	0.0	1.023882746239433	2.868140624413335E-4
42-43	0.2451782210435999	5.823194601081619E-4	0.0	1.2952957626611896	2.9550539766682845E-4
44-45	0.25675073389634645	6.64887144750364E-4	0.0	1.5946470761652995	2.9550539766682845E-4
46-47	0.26172217764532957	7.126894884905863E-4	0.0	1.942878458977593	2.9550539766682845E-4
48-49	0.26522913140881677	7.170351561033338E-4	0.0	2.2945820302124718	3.041967328923234E-4
50-51	0.2714043250865309	8.169855111965256E-4	0.0	2.6765053740263967	3.1288806811781834E-4
52-53	0.2733511841770418	8.169855111965256E-4	0.0	3.0646864792026776	3.5199907663254566E-4
54-55	0.279061391420192	8.169855111965256E-4	0.0	3.430370063147765	3.780730823090305E-4
56-57	0.2924025909913267	8.169855111965256E-4	0.0	3.839223509157886	3.9111008514727293E-4
58-59	0.29605295178603463	8.213311788092732E-4	0.0	4.278527048130528	3.954557527600204E-4
60-61	0.298421340634982	8.387138492602631E-4	0.0	4.714753854433345	3.998014203727679E-4
62-63	0.3003768910607184	8.604421873240005E-4	0.0	5.157099360734911	4.171840908237578E-4
64-65	0.3018587637166652	0.0010342688918338996	0.0	5.609379063199218	4.171840908237578E-4
66-67	0.3037187094549212	0.001038614559446647	0.0	6.084351841937291	4.171840908237578E-4
68-69	0.3054395938295692	0.001073379900348627	0.0	6.615601016260445	4.215297584365053E-4
70-71	0.3078297110165803	0.0010907625707996167	0.0	7.2107401958262125	4.4325809650024267E-4
72-73	0.31453507614304965	0.0010951082384123643	0.0	7.775585726463516	4.693321021767275E-4
74-75	0.3205320974486412	0.001147256249765334	0.0	8.33008422251487	4.693321021767275E-4
76-77	0.32227905582896565	0.0012124412639565462	0.0	8.92951692168203	4.7802343740222246E-4
78-79	0.3236088301184664	0.001229823934407536	0.0	9.554515183414985	4.7802343740222246E-4
80-81	0.3263552920497228	0.0012385152696330311	0.0	10.237080504014006	4.997517754659598E-4
82-83	0.32731568459214	0.001247206604858526	0.0	10.968851818992167	5.475541192061822E-4
84-85	0.3311789830998725	0.0012515522724712734	0.0	11.675978852938435	5.562454544316771E-4
86-87	0.3382580756410381	0.0012689349429222633	0.0	12.390237127437224	5.562454544316771E-4
88	0.3398138246464017	0.0013384656247262228	0.0	12.948012256868589	5.64936789657172E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTAAC	1535	0.0	23.879953	1
GTATAAG	4650	0.0	20.414824	1
TAGTACT	5085	0.0	19.965399	4
GTACTAG	4455	0.0	19.62061	1
TATTCGC	800	0.0	18.806475	9
CATGGGG	24900	0.0	18.234465	4
ATTGCGT	2935	0.0	17.460106	6
TCTACAC	8730	0.0	17.279922	3
CTAGTAC	6040	0.0	17.11736	3
GTGATCG	3745	0.0	17.073969	8
TAGGCAT	6540	0.0	17.036123	5
GATCTAC	7295	0.0	16.942507	1
ATAAGGT	4290	0.0	16.869976	3
CTACACT	11125	0.0	16.772804	4
GTATCAA	59060	0.0	16.638258	1
TACACTC	7940	0.0	16.578213	5
CTAACGC	2585	0.0	16.361872	3
CTATTGA	5580	0.0	16.261871	9
TGATCGC	3900	0.0	16.15428	9
CTGTGCG	6665	0.0	16.154099	9
>>END_MODULE