FastQCFastQC Report
Fri 27 May 2016
ERR765462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209888
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA172830.23971246155280085No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT155280.21537089064351625No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATA22050.024.4623181
GTATAAG26650.021.4613041
GTACTAG25800.020.546291
TAGGCAT34900.019.0528015
GTATCAA373000.017.8892151
TAGTACT27950.017.634874
GTACAAG43600.017.1706851
TCTAACG10050.017.1192152
GTACAAA44850.017.0031591
GTATTAG25350.016.8755571
GTACATG522150.016.8044721
ATTTAGG35150.016.5360931
CGTTAAC6800.016.4115871
TACATGA31000.016.3498212
TACATGG513500.016.2997252
CATGGGG186550.016.2517664
CTAGGCA42400.016.1212864
GGGCTAT37650.015.9320986
GTGATCG19400.015.8194158
TGATCGC19900.015.6556099
Produced by FastQC (version 0.11.5)