FastQCFastQC Report
Fri 27 May 2016
ERR765462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7209888
Sequences flagged as poor quality0
Sequence length99
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT248220.3442771926554199No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT169630.23527411244113638No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA168580.23381777913887147No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155820.2161198620561096No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126610.17560605657119777No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG7150.035.1198962
GTATCAA484800.030.278911
ATCAACG601100.024.1364463
GTACTAG24100.024.1321751
CAACGCA601800.024.0929185
TATCAAC620300.023.629252
AACGCAG628100.023.341926
TCAACGC622850.023.330934
AGTACTT367100.022.41047712-13
ACGCAGA675250.021.5261217
CGCAGAG680700.021.3332798
TCACGCA13100.021.2983254
TAGGCAT33550.020.929075
GGTATCA327550.020.8522051
GCAGAGT713500.020.287699
ATCACGC13600.020.1733763
TACTTTT420900.019.55160114-15
GAGTACT353550.019.11929312-13
TAACGCA6750.018.6005364
AGAGTAC689800.018.4253210-11
Produced by FastQC (version 0.11.5)