##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7209888 Sequences flagged as poor quality 0 Sequence length 99 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.37071283215495 33.0 33.0 33.0 33.0 33.0 2 32.326922692835176 33.0 33.0 33.0 33.0 33.0 3 32.3516151707211 33.0 33.0 33.0 33.0 33.0 4 36.245321563941076 37.0 37.0 37.0 33.0 37.0 5 36.162848992938585 37.0 37.0 37.0 33.0 37.0 6 36.1698712934237 37.0 37.0 37.0 33.0 37.0 7 36.06848164631683 37.0 37.0 37.0 33.0 37.0 8 36.08074064396007 37.0 37.0 37.0 33.0 37.0 9 36.10534435486377 37.0 37.0 37.0 33.0 37.0 10-11 36.05525418702759 37.0 37.0 37.0 33.0 37.0 12-13 36.030893128991735 37.0 37.0 37.0 33.0 37.0 14-15 37.50449570090409 38.5 37.0 40.0 33.0 40.0 16-17 37.510172488116325 40.0 37.0 40.0 33.0 40.0 18-19 37.56571072948706 40.0 37.0 40.0 33.0 40.0 20-21 37.5449638746122 40.0 37.0 40.0 33.0 40.0 22-23 37.5162316668442 40.0 37.0 40.0 33.0 40.0 24-25 37.487495228774705 40.0 37.0 40.0 33.0 40.0 26-27 37.354725829305536 40.0 37.0 40.0 33.0 40.0 28-29 37.25316627664674 37.0 37.0 40.0 33.0 40.0 30-31 37.088582027903904 37.0 37.0 40.0 33.0 40.0 32-33 36.95526366012898 37.0 37.0 40.0 33.0 40.0 34-35 36.84465070747285 37.0 37.0 40.0 33.0 40.0 36-37 36.689009246745584 37.0 37.0 40.0 33.0 40.0 38-39 36.57011918909143 37.0 37.0 40.0 33.0 40.0 40-41 36.39556321818037 37.0 37.0 40.0 33.0 40.0 42-43 36.23091239420086 37.0 37.0 40.0 33.0 40.0 44-45 35.9270728616034 37.0 37.0 40.0 30.0 40.0 46-47 35.66828680279083 37.0 37.0 40.0 27.0 40.0 48-49 35.373583057046105 37.0 37.0 40.0 27.0 40.0 50-51 35.16156055406131 37.0 33.0 40.0 27.0 40.0 52-53 35.005274631173194 37.0 33.0 40.0 27.0 40.0 54-55 34.81707746361663 37.0 33.0 40.0 27.0 40.0 56-57 34.61570464062687 37.0 33.0 40.0 27.0 40.0 58-59 34.40789551793315 37.0 33.0 40.0 27.0 40.0 60-61 34.23813552998327 37.0 33.0 37.0 27.0 40.0 62-63 34.022389321443 37.0 33.0 37.0 27.0 40.0 64-65 33.79612928800003 37.0 33.0 37.0 27.0 40.0 66-67 33.56799460685103 37.0 33.0 37.0 27.0 40.0 68-69 33.36301125620814 37.0 33.0 37.0 27.0 40.0 70-71 33.174283012440696 37.0 33.0 37.0 24.5 37.0 72-73 32.96662465491836 37.0 33.0 37.0 22.0 37.0 74-75 32.72906375244664 37.0 33.0 37.0 22.0 37.0 76-77 32.55167257799289 37.0 33.0 37.0 22.0 37.0 78-79 32.380148276921915 33.0 33.0 37.0 22.0 37.0 80-81 32.20598995435158 33.0 33.0 37.0 22.0 37.0 82-83 32.10716352320591 33.0 33.0 37.0 22.0 37.0 84-85 31.979995042918837 33.0 33.0 37.0 22.0 37.0 86-87 31.867548774682767 33.0 33.0 37.0 22.0 37.0 88-89 31.748944782498704 33.0 33.0 37.0 22.0 37.0 90-91 31.640796223186825 33.0 33.0 37.0 22.0 37.0 92-93 31.563713403037603 33.0 33.0 37.0 22.0 37.0 94-95 31.46261134708334 33.0 33.0 37.0 22.0 37.0 96-97 31.40267955618728 33.0 33.0 37.0 22.0 37.0 98-99 30.614276463101785 33.0 30.0 37.0 18.5 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.02292323804381624 1101 2 0.08761003234715758 1101 3 0.08023249111958819 1101 4 0.12614394687793862 1101 5 0.07255231799688033 1101 6 0.11550801576427006 1101 7 0.12182466134183301 1101 8 0.1273697448113893 1101 9 0.15131367421050612 1101 10-11 0.08538258625554107 1101 12-13 0.0685222709612745 1101 14-15 0.09887505037007571 1101 16-17 0.1688737055320999 1101 18-19 0.1605698484312441 1101 20-21 0.1925886387172966 1101 22-23 0.15311186125997267 1101 24-25 0.22261060406264477 1101 26-27 0.20846066395270668 1101 28-29 0.17263740715610965 1101 30-31 0.1431105672882822 1101 32-33 0.21702488148398658 1101 34-35 0.2776915138696481 1101 36-37 0.36248760911035305 1101 38-39 0.22234109286449666 1101 40-41 0.09650973042841571 1101 42-43 0.1786787691713343 1101 44-45 0.21300948284959986 1101 46-47 0.23562833563112662 1101 48-49 0.11358198117863338 1101 50-51 0.1469746406709831 1101 52-53 0.1594342266689992 1101 54-55 0.05789324085989733 1101 56-57 0.22150056008641883 1101 58-59 0.181528549453283 1101 60-61 0.09479798805066508 1101 62-63 0.14327956518572904 1101 64-65 0.09068948076561156 1101 66-67 0.20178584953139023 1101 68-69 0.1816278224280481 1101 70-71 0.13531812118701936 1101 72-73 0.1486497974730696 1101 74-75 0.15783431724165098 1101 76-77 0.23507004855004254 1101 78-79 0.23527956174515197 1101 80-81 0.18092203000352924 1101 82-83 0.21180339147229432 1101 84-85 0.15957632159654977 1101 86-87 0.1713255971363168 1101 88-89 0.173664608064513 1101 90-91 0.19519748411980942 1101 92-93 0.09194612562983906 1101 94-95 0.22005496361868282 1101 96-97 0.21326425079286437 1101 98-99 0.2702571114460248 1102 1 0.05129743665587938 1102 2 0.09855814057976175 1102 3 0.12434814233583324 1102 4 0.0779022767031563 1102 5 0.09371687485618452 1102 6 0.12767092617026066 1102 7 0.08245058799404603 1102 8 0.16138042332462987 1102 9 0.08644391897306036 1102 10-11 0.09604854899404103 1102 12-13 0.08519758371708264 1102 14-15 0.16949132586180582 1102 16-17 0.14635178660439152 1102 18-19 0.1671664069886063 1102 20-21 0.17169937615597064 1102 22-23 0.1601601517567346 1102 24-25 0.22845050517435084 1102 26-27 0.1934739346422063 1102 28-29 0.1400071801877587 1102 30-31 0.20281891333114999 1102 32-33 0.1682300094590019 1102 34-35 0.23725076860117866 1102 36-37 0.18517749323419253 1102 38-39 0.24758596252208065 1102 40-41 0.2127353382361008 1102 42-43 0.2069583490687137 1102 44-45 0.25070879549152636 1102 46-47 0.3212953651618946 1102 48-49 0.2869852905831536 1102 50-51 0.28892345541061815 1102 52-53 0.2807622735265767 1102 54-55 0.21908158108345788 1102 56-57 0.2568958593165789 1102 58-59 0.3204614968914967 1102 60-61 0.22310084482236192 1102 62-63 0.2952096098830381 1102 64-65 0.1617820906098828 1102 66-67 0.2689054791177341 1102 68-69 0.23254140750923114 1102 70-71 0.1793831406026385 1102 72-73 0.18704434749011511 1102 74-75 0.3305749088129559 1102 76-77 0.33368509086086817 1102 78-79 0.25562558548800496 1102 80-81 0.2543915578947349 1102 82-83 0.22344368541800463 1102 84-85 0.24950983250051095 1102 86-87 0.3065948223232837 1102 88-89 0.3471167354649225 1102 90-91 0.24448473966062068 1102 92-93 0.27844439178723945 1102 94-95 0.3672332611062501 1102 96-97 0.3545510353977548 1102 98-99 0.2854219976441321 1103 1 0.026834757202763626 1103 2 0.10676088236971282 1103 3 0.09144610956145272 1103 4 0.06968167376739132 1103 5 -0.011966873411616064 1103 6 0.050987764468914065 1103 7 0.08546589799965432 1103 8 0.14889117575978617 1103 9 0.08184309595244343 1103 10-11 0.11650052126982047 1103 12-13 0.11621367071215616 1103 14-15 0.11253212340943008 1103 16-17 0.12062008585593986 1103 18-19 0.14896835732420044 1103 20-21 0.09346439732460965 1103 22-23 0.15159690418718696 1103 24-25 0.17399497677913445 1103 26-27 0.18315169841930867 1103 28-29 0.14428717523232137 1103 30-31 0.21422735004971827 1103 32-33 0.17716860761169784 1103 34-35 0.24097182654349325 1103 36-37 0.2001663366599331 1103 38-39 0.1180067129057889 1103 40-41 0.09400974681176422 1103 42-43 0.12757799359112454 1103 44-45 0.22098338992125832 1103 46-47 0.17615921407247725 1103 48-49 0.17430904756712806 1103 50-51 0.27661972770064125 1103 52-53 0.24202363965835616 1103 54-55 0.1577802757036011 1103 56-57 0.12881851123120924 1103 58-59 0.23620339871482798 1103 60-61 0.22644442424302724 1103 62-63 0.17580008294923033 1103 64-65 0.1726662337418432 1103 66-67 0.2404513116216762 1103 68-69 0.20742338645581526 1103 70-71 0.021301127805323006 1103 72-73 0.14489061769958766 1103 74-75 0.24016824022090333 1103 76-77 0.19677966204893949 1103 78-79 0.12012276428153257 1103 80-81 0.20823814982482247 1103 82-83 0.14843645085016277 1103 84-85 0.17882425911296096 1103 86-87 0.16797834904688358 1103 88-89 0.21812683597249105 1103 90-91 0.14070027168932597 1103 92-93 0.20196996689201896 1103 94-95 0.1987478965934315 1103 96-97 0.24146464598514683 1103 98-99 0.25207383389091476 1104 1 -0.013818634458630186 1104 2 0.05317352264948738 1104 3 -0.01589155101748929 1104 4 -0.015645774946086988 1104 5 0.010926062151810356 1104 6 0.047738183136765144 1104 7 0.09731152677146326 1104 8 0.08357300125144462 1104 9 0.0054091614546223354 1104 10-11 -0.0041571869877685685 1104 12-13 -0.02945694867869264 1104 14-15 0.015461636122502398 1104 16-17 0.04128106317612179 1104 18-19 0.06432642617193807 1104 20-21 0.09276391682615781 1104 22-23 0.099739037294313 1104 24-25 0.10464793222418933 1104 26-27 0.06290076701806413 1104 28-29 0.042544232401191096 1104 30-31 -0.12619530518377786 1104 32-33 -0.01925592365925155 1104 34-35 -0.012918690732796279 1104 36-37 0.06100876768949348 1104 38-39 0.038858240479598294 1104 40-41 0.03804756799716813 1104 42-43 0.08687436281201144 1104 44-45 0.05550642396154615 1104 46-47 -0.07809443282668127 1104 48-49 -0.029317507624760708 1104 50-51 -0.1337919576135036 1104 52-53 -0.046247918631841856 1104 54-55 -0.11355833195630538 1104 56-57 0.01653807665528717 1104 58-59 -0.0194980051593987 1104 60-61 -0.0431687609180571 1104 62-63 -0.02800162436670206 1104 64-65 -0.001980158388072084 1104 66-67 -0.009024179189545123 1104 68-69 0.005501620525102169 1104 70-71 -0.12494430389089928 1104 72-73 -0.07118302770147977 1104 74-75 -0.028745578022181917 1104 76-77 -0.05855771621816075 1104 78-79 -0.07202744845329079 1104 80-81 -0.03457167884609191 1104 82-83 0.02256566294258988 1104 84-85 0.04211977986235027 1104 86-87 0.058880757775142456 1104 88-89 0.0312997809545017 1104 90-91 -0.06170281698858915 1104 92-93 0.04088274784132295 1104 94-95 -0.08849115502924221 1104 96-97 -0.1130710926000269 1104 98-99 0.05030922360029422 1105 1 -0.015444721664863437 1105 2 0.05146568902802784 1105 3 0.04300310516957495 1105 4 0.051469094914125435 1105 5 0.053171107905669146 1105 6 0.014815080142049908 1105 7 0.041922872256037635 1105 8 0.09779075284742333 1105 9 -0.011868588770646227 1105 10-11 -0.024791880328365323 1105 12-13 -0.021199759305041255 1105 14-15 0.009372144027743445 1105 16-17 0.00557213683539004 1105 18-19 0.020001630133883452 1105 20-21 0.008070454747539202 1105 22-23 0.032979372058782985 1105 24-25 0.04003421584048539 1105 26-27 -0.003941268908718598 1105 28-29 0.035317132221052816 1105 30-31 0.03732326400795216 1105 32-33 0.04550971113987856 1105 34-35 0.083515977751496 1105 36-37 0.09222408767972468 1105 38-39 0.03465388483488141 1105 40-41 -0.035647154342903775 1105 42-43 -0.016632517907773092 1105 44-45 0.0797564938214066 1105 46-47 0.06703266083061976 1105 48-49 0.05370113997266657 1105 50-51 0.020458947089004198 1105 52-53 -0.04366877283244719 1105 54-55 -0.034892103092730054 1105 56-57 0.06822428501763511 1105 58-59 0.11632564624324715 1105 60-61 0.04021083633076472 1105 62-63 0.04600018272680728 1105 64-65 0.05766220332305494 1105 66-67 0.08798318662645244 1105 68-69 0.0614195683158556 1105 70-71 0.053463687287710115 1105 72-73 0.048519091735883535 1105 74-75 0.09336569853894616 1105 76-77 0.11744009706101366 1105 78-79 0.06868033613719149 1105 80-81 0.023710587864400168 1105 82-83 -0.007401577413503446 1105 84-85 -0.0705050909504763 1105 86-87 0.030893417283149205 1105 88-89 0.09136716497169317 1105 90-91 0.07064457021544612 1105 92-93 0.0395697856809214 1105 94-95 -0.02858097890139888 1105 96-97 0.0806528360722858 1105 98-99 0.13152610934229259 1106 1 -0.06262439368723705 1106 2 0.0166045548787892 1106 3 -0.047333822893939725 1106 4 -0.017073090572310434 1106 5 0.03840537491106488 1106 6 0.046747405952707766 1106 7 -0.04417868004824754 1106 8 -0.038229552904908815 1106 9 0.025909810651576493 1106 10-11 -0.04967767816109614 1106 12-13 -0.04859580707024236 1106 14-15 -0.027919710288465183 1106 16-17 -0.06100596669305958 1106 18-19 -0.07485426137669293 1106 20-21 -0.06536067552433167 1106 22-23 -0.04974308637419256 1106 24-25 -0.045324235312278915 1106 26-27 -0.0771366141316463 1106 28-29 -0.14370803012939604 1106 30-31 -0.07667592139073776 1106 32-33 -0.1232313460412442 1106 34-35 -0.06430231926612606 1106 36-37 -0.0654678183128965 1106 38-39 -0.17341763313009295 1106 40-41 -0.15426267742491717 1106 42-43 -0.0870286778754874 1106 44-45 -0.07053581801105224 1106 46-47 -0.025842282477107403 1106 48-49 -0.07313164369009684 1106 50-51 -0.09850982151665022 1106 52-53 -0.09859316526367934 1106 54-55 -0.17505492651092425 1106 56-57 -0.01004004746725684 1106 58-59 -0.08625009265234951 1106 60-61 -0.16859027511645763 1106 62-63 -0.151154432039192 1106 64-65 -0.06685439412162708 1106 66-67 -0.08795577899029894 1106 68-69 -0.10134088473708402 1106 70-71 -0.1166031513010708 1106 72-73 -0.0384626519276452 1106 74-75 -0.08858725316477489 1106 76-77 -0.06111451417685032 1106 78-79 -0.08963222973206086 1106 80-81 -0.07653413816925081 1106 82-83 -0.16347096532418703 1106 84-85 -0.08426064266776123 1106 86-87 -0.06962358162544291 1106 88-89 -0.059667530646635214 1106 90-91 -0.219460759605969 1106 92-93 -0.04842513915269109 1106 94-95 -0.10963626324020126 1106 96-97 -0.12223486721406118 1106 98-99 -0.012418502789998342 1107 1 -0.015692265868800348 1107 2 0.04952944786099067 1107 3 0.01731873190740174 1107 4 -0.046833615331749456 1107 5 0.03472198762985812 1107 6 -0.018593099334438534 1107 7 0.02247003569516437 1107 8 0.041042081211706716 1107 9 0.036921608692402685 1107 10-11 -0.04995545313194327 1107 12-13 -0.061091799750293774 1107 14-15 0.0022108809249843375 1107 16-17 -0.0637822660042886 1107 18-19 -0.09498739221969998 1107 20-21 -0.009351906263496801 1107 22-23 0.026832833136985812 1107 24-25 0.002944465399913554 1107 26-27 -0.026816525814886916 1107 28-29 0.017453970088837423 1107 30-31 -0.05971651462932215 1107 32-33 0.030569545726713443 1107 34-35 -0.04423126064161664 1107 36-37 0.07461532036703034 1107 38-39 -0.09438214280324075 1107 40-41 -0.12356406444772716 1107 42-43 -0.08579372044687972 1107 44-45 -0.10902300018160815 1107 46-47 -0.02555052950251735 1107 48-49 -0.06164092768255358 1107 50-51 -0.039988717265181606 1107 52-53 -0.09131351016366551 1107 54-55 -0.14976767375225108 1107 56-57 -0.06596483713655488 1107 58-59 -0.08978975513439735 1107 60-61 -0.060750972304219886 1107 62-63 -0.10634260163060105 1107 64-65 -0.10080918865182298 1107 66-67 0.007849546402802332 1107 68-69 -0.101277003506695 1107 70-71 -0.022591895517528826 1107 72-73 -0.09704196332815229 1107 74-75 -0.07103650671545836 1107 76-77 -0.03675169836999714 1107 78-79 -0.024745730644383457 1107 80-81 -0.10497892156391231 1107 82-83 -0.06425236123553901 1107 84-85 -0.045408948586302955 1107 86-87 -0.09615192013935214 1107 88-89 -0.05527997552636066 1107 90-91 -0.005054327645872547 1107 92-93 0.06753553525587108 1107 94-95 -0.013426834874248783 1107 96-97 0.017543735869828936 1107 98-99 0.0901803715041396 1108 1 -0.023649464572208956 1108 2 0.028285630453488864 1108 3 0.055245183935731745 1108 4 -0.01631455120591596 1108 5 0.0635554646019898 1108 6 -0.02748360463169064 1108 7 -0.09377476536437968 1108 8 -0.08683340996193323 1108 9 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64-65 0.018338227573948984 2215 66-67 -0.05648915443926228 2215 68-69 0.03651930930590197 2215 70-71 -0.08749531774189023 2215 72-73 -0.09919454927987204 2215 74-75 -0.154691814273761 2215 76-77 -0.06547261276262617 2215 78-79 -0.0515677519245159 2215 80-81 -0.020088024013780625 2215 82-83 -0.03514865763148123 2215 84-85 -0.03936416014910549 2215 86-87 -0.09001878715522338 2215 88-89 -0.052466743293130946 2215 90-91 -0.072491103606108 2215 92-93 0.026280110752054497 2215 94-95 -0.011082447430258213 2215 96-97 -0.023270285463215856 2215 98-99 -0.13082271124677547 2216 1 -0.05944898695874912 2216 2 -0.08280089201362983 2216 3 -0.0575774599891119 2216 4 -0.16470282961385152 2216 5 -0.12619166305289298 2216 6 -0.18404719134050396 2216 7 -0.06808885588227298 2216 8 -0.1813218280656912 2216 9 -0.12104775064427997 2216 10-11 -0.04425031324346662 2216 12-13 -0.05282399866685239 2216 14-15 -0.25108594098987425 2216 16-17 -0.22598569600724971 2216 18-19 -0.13854368807756856 2216 20-21 -0.16579462460327932 2216 22-23 -0.20695734687788558 2216 24-25 -0.24265739016338017 2216 26-27 -0.2293901554773825 2216 28-29 -0.16707956155318726 2216 30-31 -0.20340342504940878 2216 32-33 -0.19246439046253272 2216 34-35 -0.1553441453785993 2216 36-37 -0.19641551926972056 2216 38-39 -0.21193895971997279 2216 40-41 -0.13341537429293737 2216 42-43 -0.16992105902924237 2216 44-45 -0.22473404383639206 2216 46-47 -0.14191779741985755 2216 48-49 -0.10931863512779927 2216 50-51 -0.19959928879429611 2216 52-53 -0.1574114160871929 2216 54-55 -0.04853549588313655 2216 56-57 -0.145130657986698 2216 58-59 -0.19588551495487394 2216 60-61 -0.18026289306247634 2216 62-63 -0.0609924712639085 2216 64-65 -0.13298825602274889 2216 66-67 -0.18402763911109332 2216 68-69 -0.04673430871852702 2216 70-71 -0.10683390444221885 2216 72-73 -0.15096821296151575 2216 74-75 -0.08754716638323146 2216 76-77 -0.13791051469785032 2216 78-79 0.013498082015317436 2216 80-81 -0.07592348083292677 2216 82-83 -0.1499218785328864 2216 84-85 -0.13802635410522512 2216 86-87 -0.24614557899406364 2216 88-89 -0.20235398809375482 2216 90-91 -0.14887860594081914 2216 92-93 -0.021240512942387824 2216 94-95 -0.10392296653484223 2216 96-97 -0.12982781620855377 2216 98-99 -0.17136002426830999 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 13.0 10 61.0 11 153.0 12 303.0 13 589.0 14 1637.0 15 4146.0 16 9164.0 17 17374.0 18 28167.0 19 38954.0 20 49187.0 21 62069.0 22 75049.0 23 72797.0 24 59929.0 25 55699.0 26 63331.0 27 80605.0 28 104509.0 29 137455.0 30 178041.0 31 233306.0 32 305330.0 33 402451.0 34 534747.0 35 767958.0 36 1281338.0 37 1946355.0 38 698574.0 39 596.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.86260069543129 18.73109879032258 13.172616615745348 25.23368389850078 2 16.00887558863605 20.898091620840713 37.496712847689174 25.59631994283406 3 17.99758886684509 26.103290370113935 29.665398408408006 26.23372235463297 4 13.14088374188337 17.275247548921705 36.40156407422695 33.18230463496798 5 14.072406211144974 36.972743710652566 33.31580089138042 15.63904918682204 6 31.474830122187754 36.922237904389085 17.191764976099492 14.411166997323676 7 27.059435597335213 31.963603318109797 22.323689355507327 18.653271729047663 8 27.432395621124765 32.17620024055852 20.027745229884292 20.36365890843242 9 27.427804703762387 13.928343963179458 19.89779868979934 38.746052643258814 10-11 25.47036791694961 24.964264354730613 27.25140113133519 22.313966596984585 12-13 26.845618128880783 22.990107474623738 27.2521917122707 22.912082684224778 14-15 24.20009370602329 23.358403901810068 25.78913620411824 26.652366188048404 16-17 23.12556678168977 25.87458107741853 26.497461844443897 24.502390296447803 18-19 23.524764101302647 24.54264644415954 29.31216119566899 22.62042825886882 20-21 24.52409015747016 23.89429413052156 28.674155748075485 22.907459963932794 22-23 23.986190908929515 23.699716278533035 28.511254266363085 23.802838546174364 24-25 23.588431609478537 24.242637333617388 28.557184244748324 23.61174681215575 26-27 23.773309654740824 24.10943138090356 28.44599666458064 23.671262299774977 28-29 23.44720195376128 24.022606176406626 28.88739048373567 23.642801386096426 30-31 23.961711658918723 23.91172463418555 28.607828073484253 23.518735633411474 32-33 23.575664420862015 24.08822439405439 28.261819046287545 24.074292138796054 34-35 23.850370491192095 24.05077582342472 28.638288139843503 23.46056554553968 36-37 24.10608181430835 24.032571657146406 28.056524595111604 23.80482193343364 38-39 24.176096771544856 23.752206691698955 28.14660227731693 23.92509425943926 40-41 23.932098529131103 23.93752163695192 28.308151250061027 23.82222858385595 42-43 24.38915833366621 23.970545728310896 27.722587368902264 23.91770856912063 44-45 24.138017123150874 24.28241603753068 27.26717114052257 24.31239569879588 46-47 24.055692682049983 24.300301197466588 27.20161533715919 24.442390783324235 48-49 24.759219560692205 24.499624682103242 26.413461623814406 24.327694133390143 50-51 24.104819658779718 24.9628357611103 26.030258722465593 24.90208585764439 52-53 23.999499021344022 25.025756294688627 25.798743336928396 25.176001347038955 54-55 24.19986967897421 24.963848259501393 25.790650284720094 25.0456317768043 56-57 24.22384369909768 24.60753897980107 25.788750116506666 25.379867204594582 58-59 23.921101404148125 24.577748265836323 26.30645762503229 25.19469270498326 60-61 24.08509674491476 24.80685552951724 26.032498701782885 25.075549023785115 62-63 23.761298372457382 24.76594643356457 25.977678155333344 25.495077038644702 64-65 23.614728827965152 24.528883111637796 26.124011912529017 25.73237614786804 66-67 24.11800294262546 24.533786100422088 26.043206219014774 25.305004737937676 68-69 24.02040780661225 24.243400174870956 26.096057248046016 25.640134770470773 70-71 23.58398632544639 24.377285749792506 26.19067730317031 25.848050621590797 72-73 24.107683780940842 24.488334631550448 26.069884858128173 25.33409672938054 74-75 24.226095438838165 24.468512637060968 25.969923651342818 25.335468272758042 76-77 23.94541572750389 24.588748299382 26.036109621257165 25.429726351856946 78-79 23.924509470297338 24.67243407297301 25.96445893134626 25.438597525383383 80-81 23.63199110443571 24.732653521335394 26.068735765637328 25.566619608591566 82-83 23.724000370879626 24.696054507931574 25.84802132460754 25.731923796581263 84-85 23.78993651600748 24.823668290417732 25.4544512652068 25.931943928367986 86-87 23.577420254374054 24.68435565376118 25.650268026969623 26.087956064895142 88-89 23.66327009194282 24.33083492657938 25.784191554858577 26.221703426619218 90-91 23.75407621642232 24.507251299652655 25.662052722325175 26.07661976159985 92-93 23.51632615965836 24.668922912658648 25.53365308029868 26.281097847384316 94-95 22.97707933689464 24.681196279316588 25.92941320392252 26.41231117986625 96-97 23.544662348349522 24.550743971395413 25.554765884490077 26.349827795764984 98-99 23.310532990153863 24.6348840534864 25.563338290914643 26.491244665445095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 87.0 1 123.0 2 446.0 3 736.0 4 525.0 5 477.5 6 973.5 7 2512.5 8 4798.5 9 4871.5 10 2923.5 11 1878.5 12 1818.0 13 2044.5 14 2431.5 15 2977.0 16 3676.0 17 4409.0 18 5386.5 19 6569.5 20 8032.5 21 9796.5 22 12099.5 23 14756.0 24 17738.5 25 21878.0 26 27199.0 27 32206.5 28 38311.0 29 47171.0 30 54885.0 31 64262.5 32 75550.0 33 85157.5 34 93660.5 35 103015.5 36 110771.5 37 122382.0 38 134939.0 39 134261.5 40 135556.0 41 144541.5 42 156822.5 43 178159.5 44 202045.5 45 226898.5 46 258842.5 47 288051.5 48 306009.5 49 325702.0 50 350288.0 51 341393.5 52 318502.0 53 315468.0 54 304915.5 55 290583.5 56 268380.0 57 247507.0 58 234698.5 59 209098.5 60 176701.0 61 141087.0 62 115011.5 63 92769.5 64 69940.5 65 53571.5 66 39379.5 67 30189.0 68 23918.5 69 19894.5 70 16862.5 71 14403.0 72 14101.0 73 11966.0 74 8324.0 75 6553.5 76 4935.5 77 3337.0 78 2048.5 79 1327.0 80 832.5 81 571.5 82 389.0 83 254.5 84 155.5 85 85.0 86 51.0 87 35.0 88 18.0 89 7.5 90 5.0 91 2.5 92 1.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.055479363895805316 2 0.0 3 0.0 4 0.0 5 0.005492457025684726 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.001470203143238841 16-17 0.004466088793612328 18-19 9.223444247677634E-4 20-21 1.941777736353186E-4 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 2.3578729655717262E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 6.934920486975665E-6 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 4.854444340882965E-5 76-77 1.2482856876556197E-4 78-79 4.854444340882965E-5 80-81 3.467460243487832E-5 82-83 2.0804761460926993E-5 84-85 5.409237979841019E-4 86-87 0.0010055634706114714 88-89 3.6755078580971023E-4 90-91 0.0010679777549942523 92-93 0.0017267952012569403 94-95 0.001158131721324936 96-97 0.0018724285314834295 98-99 7.628412535673231E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 7209888.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 37.90469558897547 #Duplication Level Percentage of deduplicated Percentage of total 1 71.6037249067612 27.14117395627524 2 14.702500142695158 11.145875846114565 3 5.299664728295917 6.026465347490613 4 2.5211318484890746 3.8225094102659756 5 1.3779488294179278 2.6115365458135815 6 0.8712385448441841 1.981441909662045 7 0.5985226890484813 1.5880774232034396 8 0.4323848575287577 1.311153312152807 9 0.3260640963839802 1.1123424284336214 >10 1.9111407771913853 13.5525171437446 >50 0.17767411082046236 4.719037093860875 >100 0.14844155157666858 11.673913915018927 >500 0.018926216354525256 4.940778196012613 >1k 0.010269917813390013 6.765987190673989 >5k 1.8339138945895237E-4 0.39923250564135443 >10k+ 1.8339138945895237E-4 1.2079577756356987 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24822 0.3442771926554199 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 16963 0.23527411244113638 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 16858 0.23381777913887147 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15582 0.2161198620561096 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12661 0.17560605657119777 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3869840973951329E-5 0.0 0.0 1.3869840973951329E-5 0.0 3 1.3869840973951329E-5 0.0 0.0 2.7739681947902658E-5 0.0 4 1.3869840973951329E-5 0.0 0.0 4.1609522921853985E-5 1.3869840973951329E-5 5 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 6 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 7 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 8 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 9 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 10-11 1.3869840973951329E-5 0.0 0.0 9.708888681765931E-5 1.3869840973951329E-5 12-13 2.0804761460926993E-5 0.0 0.0 1.178936482785863E-4 3.467460243487832E-5 14-15 2.7739681947902658E-5 0.0 0.0 1.386984097395133E-4 7.628412535673231E-5 16-17 2.7739681947902658E-5 0.0 0.0 1.941777736353186E-4 9.708888681765931E-5 18-19 2.7739681947902658E-5 0.0 0.0 3.0513650142692923E-4 9.708888681765931E-5 20-21 4.1609522921853985E-5 0.0 0.0 5.755984004189802E-4 1.0402380730463497E-4 22-23 4.1609522921853985E-5 0.0 0.0 0.0021359555099885047 1.1095872779161063E-4 24-25 4.854444340882965E-5 0.0 0.0 0.005048622114518284 1.1095872779161063E-4 26-27 8.321904584370797E-5 0.0 0.0 0.008897502984789777 1.1095872779161063E-4 28-29 8.321904584370797E-5 0.0 0.0 0.02224722492221793 1.1095872779161063E-4 30-31 8.321904584370797E-5 0.0 0.0 0.059612576506042805 1.386984097395133E-4 32-33 8.321904584370797E-5 0.0 0.0 0.1203694148924366 1.6643809168741594E-4 34-35 8.321904584370797E-5 0.0 0.0 0.1966604751696559 1.9417777363531862E-4 36-37 8.321904584370797E-5 0.0 0.0 0.31370806314883115 1.9417777363531862E-4 38-39 8.321904584370797E-5 0.0 0.0 0.49060401493060646 1.9417777363531862E-4 40-41 8.321904584370797E-5 0.0 0.0 0.7035130088012462 1.9417777363531862E-4 42-43 8.321904584370797E-5 0.0 0.0 0.8961373602474824 2.149825350962456E-4 44-45 9.708888681765931E-5 0.0 0.0 1.1143793079726065 2.4272221704414826E-4 46-47 9.708888681765931E-5 0.0 0.0 1.3509641203857812 2.8433173996600224E-4 48-49 9.708888681765931E-5 0.0 0.0 1.6010512229870977 3.4674602434878323E-4 50-51 9.708888681765931E-5 0.0 0.0 1.8856048804086831 3.4674602434878323E-4 52-53 9.708888681765931E-5 0.0 0.0 2.1619961364170983 3.814206267836616E-4 54-55 9.708888681765931E-5 0.0 0.0 2.433123787775899 4.299650701924912E-4 56-57 9.708888681765931E-5 0.0 0.0 2.7347581543569053 4.507698316534182E-4 58-59 9.708888681765931E-5 0.0 0.0 3.0656648203134362 4.715745931143452E-4 60-61 9.708888681765931E-5 0.0 0.0 3.4030140274023672 4.715745931143452E-4 62-63 9.708888681765931E-5 0.0 0.0 3.7452246137526686 4.715745931143452E-4 64-65 9.708888681765931E-5 0.0 0.0 4.103614369599084 4.715745931143452E-4 66-67 9.708888681765931E-5 0.0 0.0 4.482649383735225 4.854444340882965E-4 68-69 9.708888681765931E-5 0.0 0.0 4.892621078163766 4.993142750622479E-4 70-71 9.708888681765931E-5 0.0 0.0 5.353495088966708 4.993142750622479E-4 72-73 9.708888681765931E-5 0.0 0.0 5.80219137939452 5.755984004189802E-4 74-75 9.708888681765931E-5 0.0 0.0 6.24642158102872 5.825333209059558E-4 76-77 9.708888681765931E-5 0.0 0.0 6.719598140775557 5.825333209059558E-4 78-79 9.708888681765931E-5 0.0 0.0 7.212636312797092 5.894682413929315E-4 80-81 9.708888681765931E-5 0.0 0.0 7.744516974466178 5.964031618799072E-4 82-83 9.708888681765931E-5 0.0 0.0 8.315607676568625 6.102730028538585E-4 84-85 9.708888681765931E-5 0.0 0.0 8.874860469399803 6.102730028538585E-4 86-87 9.708888681765931E-5 0.0 0.0 9.43856548118362 6.241428438278098E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 715 0.0 35.119896 2 GTATCAA 48480 0.0 30.27891 1 ATCAACG 60110 0.0 24.136446 3 GTACTAG 2410 0.0 24.132175 1 CAACGCA 60180 0.0 24.092918 5 TATCAAC 62030 0.0 23.62925 2 AACGCAG 62810 0.0 23.34192 6 TCAACGC 62285 0.0 23.33093 4 AGTACTT 36710 0.0 22.410477 12-13 ACGCAGA 67525 0.0 21.526121 7 CGCAGAG 68070 0.0 21.333279 8 TCACGCA 1310 0.0 21.298325 4 TAGGCAT 3355 0.0 20.92907 5 GGTATCA 32755 0.0 20.852205 1 GCAGAGT 71350 0.0 20.28769 9 ATCACGC 1360 0.0 20.173376 3 TACTTTT 42090 0.0 19.551601 14-15 GAGTACT 35355 0.0 19.119293 12-13 TAACGCA 675 0.0 18.600536 4 AGAGTAC 68980 0.0 18.42532 10-11 >>END_MODULE