FastQCFastQC Report
Fri 27 May 2016
ERR765460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18056376
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA1145880.6346123939820483No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT1080430.5983648103030198No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG463700.25680679223782227No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG386610.21411273225590785No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT358560.19857805353632424No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG344000.1905144199478345No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC341470.18911325284763675No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC334580.18529742623879786No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT302570.16756961640586127No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA261670.14491833798764492No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA259430.1436777789740311No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC242860.13450096519921828No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT215370.11927642623303812No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC213200.11807463468859974No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA210420.1165350123413469No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC210320.11647963024252485No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA209580.11606980271124173No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC204220.11310132221438012No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC204140.11305701653532248No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT202770.11229828178146047No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC193980.10743019529500272No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG181590.10056835325095136No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT180920.10019729318884366No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACAAG51750.032.9618841
GTACATA37150.029.2192381
CTATTGA110550.025.7120449
AGGGCTA114450.024.7567255
GTACTAG94450.024.3289091
CGTTAAC31550.024.27751
TATTCTC64050.024.2095385
GTATAAG100600.023.3087141
GTGATCG76650.023.2309538
TTTAGGG129600.022.5522732
TATTCGC15950.022.3868459
GGGCTAT135100.022.3612256
ATTTAGG132300.022.3056831
GGCTATT135700.021.5352787
TACATGA53900.021.1847062
TGATCGC84950.020.9611849
TAGGCAT137850.020.861065
GTACAAA53150.020.7768821
ATAAGGT92700.020.5801643
ACGTTAA21400.020.417818
Produced by FastQC (version 0.11.5)