##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765460_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	18056376
Sequences flagged as poor quality	0
Sequence length	100
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.82675416152167	33.0	33.0	33.0	27.0	33.0
2	31.720555941014965	33.0	33.0	33.0	27.0	33.0
3	31.76335345475748	33.0	33.0	33.0	27.0	33.0
4	35.448200403004456	37.0	37.0	37.0	33.0	37.0
5	35.57910014722777	37.0	37.0	37.0	33.0	37.0
6	35.5580454793365	37.0	37.0	37.0	33.0	37.0
7	35.49044791712357	37.0	37.0	37.0	33.0	37.0
8	35.461701007998506	37.0	37.0	37.0	33.0	37.0
9	35.39272670219096	37.0	37.0	37.0	33.0	37.0
10-11	35.40355163184462	37.0	37.0	37.0	33.0	37.0
12-13	35.36076560988761	37.0	37.0	37.0	33.0	37.0
14-15	36.92178181269597	40.0	37.0	40.0	33.0	40.0
16-17	36.98032066345982	40.0	37.0	40.0	33.0	40.0
18-19	36.981950835538655	40.0	37.0	40.0	33.0	40.0
20-21	37.090538378243785	40.0	37.0	40.0	33.0	40.0
22-23	37.11000524136183	40.0	37.0	40.0	33.0	40.0
24-25	37.11156081375354	40.0	37.0	40.0	33.0	40.0
26-27	37.078435174367215	40.0	37.0	40.0	33.0	40.0
28-29	37.03058102578281	40.0	37.0	40.0	33.0	40.0
30-31	36.93080627031692	38.5	37.0	40.0	33.0	40.0
32-33	36.78285587318297	37.0	37.0	40.0	33.0	40.0
34-35	36.66175488370423	37.0	37.0	40.0	33.0	40.0
36-37	36.561612723394774	37.0	37.0	40.0	33.0	40.0
38-39	36.424741238219674	37.0	37.0	40.0	33.0	40.0
40-41	36.280686196388466	37.0	37.0	40.0	33.0	40.0
42-43	36.10441907058205	37.0	37.0	40.0	30.0	40.0
44-45	35.90040147037257	37.0	37.0	40.0	27.0	40.0
46-47	35.65226012130009	37.0	37.0	40.0	27.0	40.0
48-49	35.362146839432235	37.0	33.0	40.0	27.0	40.0
50-51	35.22905797929773	37.0	33.0	40.0	27.0	40.0
52-53	35.06384041293779	37.0	33.0	40.0	27.0	40.0
54-55	34.88519041694745	37.0	33.0	40.0	27.0	40.0
56-57	34.700943755269606	37.0	33.0	40.0	27.0	40.0
58-59	34.51172776862866	37.0	33.0	40.0	27.0	40.0
60-61	34.29600513414209	37.0	33.0	37.0	27.0	40.0
62-63	34.052842635753706	37.0	33.0	37.0	27.0	40.0
64-65	33.86794249300081	37.0	33.0	37.0	27.0	40.0
66-67	33.687830021926885	37.0	33.0	37.0	27.0	40.0
68-69	33.49675682429299	37.0	33.0	37.0	27.0	40.0
70-71	33.31066546797652	37.0	33.0	37.0	27.0	38.5
72-73	33.10471899233822	37.0	33.0	37.0	22.0	37.0
74-75	32.889646959057565	37.0	33.0	37.0	22.0	37.0
76-77	32.69368061453749	37.0	33.0	37.0	22.0	37.0
78-79	32.54439550882192	37.0	33.0	37.0	22.0	37.0
80-81	32.40954970698439	37.0	33.0	37.0	22.0	37.0
82-83	32.28922700214041	35.0	33.0	37.0	22.0	37.0
84-85	32.14626318149334	33.0	33.0	37.0	22.0	37.0
86-87	32.02728421251308	33.0	33.0	37.0	22.0	37.0
88-89	31.9052591173334	33.0	33.0	37.0	22.0	37.0
90-91	31.780204233673466	33.0	33.0	37.0	22.0	37.0
92-93	31.65335876368547	33.0	33.0	37.0	22.0	37.0
94-95	31.544597348881084	33.0	33.0	37.0	18.5	37.0
96-97	31.467762689478775	33.0	33.0	37.0	15.0	37.0
98-99	31.359457706241827	33.0	33.0	37.0	15.0	37.0
100	31.274500597462083	33.0	33.0	37.0	15.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	19.0
7	217.0
8	1239.0
9	2342.0
10	3577.0
11	6723.0
12	14412.0
13	30485.0
14	52087.0
15	62673.0
16	69851.0
17	81508.0
18	98020.0
19	112658.0
20	128443.0
21	150640.0
22	172594.0
23	162965.0
24	149335.0
25	152529.0
26	171118.0
27	200293.0
28	247242.0
29	313338.0
30	403800.0
31	523448.0
32	690840.0
33	917886.0
34	1214509.0
35	1690947.0
36	2886670.0
37	5026704.0
38	2314053.0
39	3206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.11480390568418	20.154427129853893	13.07426533005967	23.65650363440226
2	15.629253768211743	21.968849615750266	37.90900906464385	24.492887551394148
3	17.98083050105385	28.918771249019333	29.15929969380089	23.941098556125933
4	12.297116295473819	18.383992967525316	37.58616132865565	31.732729408345218
5	12.837387459018709	39.31292169103612	33.117686463832015	14.732004386113154
6	28.980261598451428	38.56132592719602	18.448452779228788	14.009959695123761
7	25.384805899035335	33.19410273689471	23.229378918560403	18.191712445509552
8	26.950563058722306	32.38559055261144	20.47324446500228	20.190601923663973
9	27.58421734239473	14.620309191611872	20.529828355368764	37.26564511062463
10-11	25.228930212795746	26.620430367644094	26.157286489824976	21.99335292973518
12-13	26.0078185124191	24.98502191137358	26.593943878882452	22.41321569732487
14-15	24.4587673628418	24.788105320801915	24.93817973218989	25.814947584166394
16-17	22.96454258926658	26.916330080730784	26.24167950151498	23.877447828487657
18-19	23.261656798887376	26.373945008637538	28.536165008646396	21.828233183828686
20-21	25.170870600952394	25.24424396264779	27.016743564656075	22.56814187174374
22-23	24.414641032966017	25.22341389344921	26.48425117174809	23.87769390183668
24-25	23.21581738993149	26.28901753441193	26.91715241054997	23.578012665106606
26-27	23.87919130217696	25.677605592478344	26.759037397421174	23.684165707923523
28-29	23.109481405037076	25.61651165901807	27.71576363944055	23.5582432965043
30-31	23.979944800424313	25.5956998062614	27.26301226826495	23.161343125049335
32-33	23.008825955789796	26.06961761761451	26.593511960935906	24.328044465659783
34-35	23.413468504776137	25.963760651985012	27.700428583012922	22.92234226022593
36-37	23.873068616233226	25.73726686340133	26.848376897492248	23.541287622873195
38-39	24.143375862193984	25.085134026248735	26.826773005020975	23.944717106536302
40-41	23.401106957061373	25.667231697697947	27.39516798196392	23.536493363276765
42-43	24.368639638542277	25.23910685581734	26.794538739312724	23.597714766327663
44-45	23.776020551559306	25.83935503075721	26.535991207381954	23.848633210301525
46-47	23.261729835462873	25.60349127879627	27.231979042268005	23.902799843472852
48-49	24.11930012438532	25.954463391298297	26.496318997149633	23.429917487166747
50-51	23.077530210923978	26.50636996898623	25.810764614505743	24.60533520558405
52-53	22.81823877402087	26.392751879341876	25.278820160058473	25.510189186578785
54-55	23.261731865455324	26.377060748119664	25.45909226428053	24.902115122144483
56-57	23.93928300089141	25.31728019380573	25.30611089630746	25.4373259089954
58-59	23.355217378167	25.330744275119415	26.34226223669693	24.97177611001666
60-61	23.575450016922197	25.669947339005084	25.859074369599643	24.895528274473072
62-63	22.84654313937626	25.57511680980457	25.595568115616572	25.9827719352026
64-65	22.528582696917592	25.71814065815766	26.013390555315507	25.739886089609232
66-67	23.94153715082249	25.33535439469372	25.620785783937627	25.10232267054616
68-69	23.854931950888883	24.912501955832116	25.592502897400855	25.64006319587814
70-71	22.964066581248716	25.304375529258138	25.883436344989047	25.8481215445041
72-73	24.110636744654652	25.134232537169428	25.66253325350093	25.09259746467499
74-75	24.294729468444118	25.143141532181666	25.74657874555176	24.815550253822458
76-77	23.352974395171387	25.302580466210195	26.28647238066374	25.057972757954676
78-79	23.4033202512463	25.59166325793565	25.948153152717808	25.05686333810025
80-81	22.784603350230068	25.42237037156829	26.68578187560735	25.107244402594294
82-83	23.34649149975877	25.303034133633002	25.960825229231805	25.389649137376423
84-85	23.450660369048357	25.584477761359963	25.20677905949606	25.758082810095623
86-87	23.07431004908541	25.009638775283076	25.9476938286888	25.968357346942717
88-89	23.235134574797097	24.356512066183637	26.10617840628631	26.30217495273296
90-91	23.527249892564875	24.60185867543726	25.930465444768124	25.940425987229737
92-93	23.31532316169437	24.899004831336054	25.841296878568286	25.944375128401287
94-95	22.321087491438707	25.08918241612771	26.453830326654337	26.135899765779246
96-97	23.114302206492276	24.764886861939956	25.693880712926166	26.426930218641605
98-99	22.88658005569984	24.93812328776379	25.699366074536943	26.475930581999425
100	22.86230852646375	25.02082961804429	25.406507081848602	26.710354773643363
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3899.0
1	2901.5
2	2420.5
3	2888.5
4	2297.5
5	1845.0
6	2382.5
7	4674.0
8	7171.0
9	6460.5
10	3852.5
11	2545.0
12	2573.0
13	2923.0
14	3408.5
15	4254.5
16	5233.5
17	6244.5
18	7711.0
19	9325.0
20	11250.0
21	14019.0
22	17404.5
23	21565.5
24	27089.5
25	34784.5
26	45667.0
27	56941.0
28	71492.5
29	98976.5
30	130710.5
31	158909.0
32	196169.0
33	236630.5
34	277006.5
35	300374.0
36	318480.0
37	366822.5
38	386360.0
39	376622.5
40	365901.5
41	356340.0
42	364102.5
43	401242.0
44	467099.5
45	541931.5
46	634407.0
47	728200.5
48	829479.0
49	917847.0
50	1057100.5
51	1052543.0
52	877912.0
53	802162.0
54	743256.5
55	674550.0
56	649486.0
57	600334.0
58	534289.5
59	489479.5
60	420272.5
61	309385.0
62	214861.0
63	162407.5
64	120457.0
65	100316.5
66	86745.5
67	60807.0
68	42003.0
69	32675.5
70	26857.5
71	25457.0
72	23424.5
73	26602.5
74	21236.5
75	14362.0
76	11073.5
77	9083.0
78	7523.0
79	4282.0
80	2991.5
81	2809.5
82	2956.0
83	2753.5
84	2157.5
85	1751.0
86	1289.0
87	663.5
88	289.0
89	225.5
90	207.5
91	141.5
92	93.5
93	66.5
94	40.0
95	28.0
96	20.5
97	14.0
98	11.5
99	11.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.017727809832936575
2	0.005876040685018965
3	0.006263715376773279
4	0.003943205436129598
5	0.0471080132580314
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.655218522254964E-4
18-19	0.0016836158041901655
20-21	5.288990437505289E-4
22-23	0.004840395437046725
24-25	0.007401817507566303
26-27	0.005834504110902431
28-29	0.022379906133988346
30-31	0.020496914774038824
32-33	0.013036946062709373
34-35	0.013646149149751865
36-37	0.013560306896577697
38-39	0.02110888696602242
40-41	0.030183243858014477
42-43	0.01802687316657562
44-45	0.0168915401407237
46-47	0.01815148288892522
48-49	0.013272319982703064
50-51	0.010046312726318946
52-53	0.027195379626565153
54-55	0.025243160643088073
56-57	0.013405237019875971
58-59	0.016418023195795214
60-61	0.01753397248705942
62-63	0.02070736674956259
64-65	0.011126263653348822
66-67	0.01564821202216879
68-69	0.0077590320449684915
70-71	0.008988514638817889
72-73	0.010445063837837672
74-75	0.003572145374021897
76-77	0.007443354081682836
78-79	0.005679434234200705
80-81	0.0023371245702902953
82-83	1.9660645081825943E-4
84-85	0.002489425342050919
86-87	0.002212514847940694
88-89	0.0010550289825599557
90-91	7.227363896276861E-4
92-93	0.0010550289825599557
94-95	0.0030266317006247545
96-97	0.0012654809580837263
98-99	0.0017168450634833922
100	0.001971602718064799
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.8056376E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.398585035018957
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.73467775591651	14.020901690586888
2	14.468340630732406	5.902673533432296
3	6.076220521349353	3.7183890298881597
4	3.039406620231448	2.4799837759516303
5	1.8214963132795383	1.8577973718703094
6	1.1762056551897775	1.4395758645635315
7	0.7876148112855821	1.124635939199455
8	0.5557815530507864	0.9069725816641091
9	0.4253596397037867	0.7809061302866448
>10	2.3412341679565176	8.856893532185806
>50	0.235853419344795	3.3660625995053963
>100	0.23011630619605403	10.106390628284888
>500	0.04687551305166476	6.77678179693222
>1k	0.0528581807259463	22.509097017917988
>5k	0.005928017203153309	8.133303140478866
>10k+	0.0020308947825617815	8.019635367251741
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	114588	0.6346123939820483	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	108043	0.5983648103030198	No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG	46370	0.25680679223782227	No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG	38661	0.21411273225590785	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	35856	0.19857805353632424	No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG	34400	0.1905144199478345	No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC	34147	0.18911325284763675	No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC	33458	0.18529742623879786	No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT	30257	0.16756961640586127	No Hit
ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA	26167	0.14491833798764492	No Hit
GTACATGGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCA	25943	0.1436777789740311	No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC	24286	0.13450096519921828	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21537	0.11927642623303812	No Hit
CCTATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTC	21320	0.11807463468859974	No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA	21042	0.1165350123413469	No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC	21032	0.11647963024252485	No Hit
GTATCAACGCAGAGTACATGGGGAATAATTGCAATCCCCGATCCCCATCA	20958	0.11606980271124173	No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC	20422	0.11310132221438012	No Hit
ATATAGTATAGCTTCCCATCTTCTTTGAGAGTTGTTGGTTTATGCTCATC	20414	0.11305701653532248	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT	20277	0.11229828178146047	No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC	19398	0.10743019529500272	No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG	18159	0.10056835325095136	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	18092	0.10019729318884366	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.6614629646613474E-5
2	1.6614629646613474E-5	0.0	0.0	0.0	1.6614629646613474E-5
3	1.6614629646613474E-5	0.0	0.0	0.0	1.6614629646613474E-5
4	1.6614629646613474E-5	0.0	0.0	0.0	1.6614629646613474E-5
5	1.6614629646613474E-5	0.0	0.0	0.0	1.6614629646613474E-5
6	2.2152839528817965E-5	0.0	0.0	0.0	3.322925929322695E-5
7	2.7691049411022457E-5	0.0	0.0	0.0	3.322925929322695E-5
8	2.7691049411022457E-5	0.0	0.0	0.0	3.322925929322695E-5
9	2.7691049411022457E-5	0.0	0.0	0.0	3.876746917543144E-5
10-11	4.430567905763593E-5	0.0	0.0	0.0	4.707478399873818E-5
12-13	8.030404329196513E-5	0.0	0.0	8.307314823306737E-6	5.5382098822044914E-5
14-15	1.1353330258519206E-4	0.0	0.0	4.430567905763593E-5	7.476583340976063E-5
16-17	1.2184061740849881E-4	0.0	0.0	8.584225317416961E-5	7.753493835086288E-5
18-19	1.273788272907033E-4	0.0	0.0	1.6614629646613473E-4	7.753493835086288E-5
20-21	1.4953166681952126E-4	0.0	0.0	3.101397534034515E-4	8.030404329196513E-5
22-23	1.7168450634833923E-4	0.0	0.0	0.001093796451735387	8.307314823306736E-5
24-25	1.993755557593617E-4	0.0	0.0	0.003447535651672296	8.307314823306736E-5
26-27	2.796795990513268E-4	0.0	0.0	0.0065738551301767305	8.307314823306736E-5
28-29	2.9352512375683805E-4	0.0	0.0	0.022008846071880646	8.307314823306736E-5
30-31	3.101397534034515E-4	0.0	0.0	0.0822590313803833	8.307314823306736E-5
32-33	3.3229259293226945E-4	0.0	0.0	0.18675951364769985	9.13804630563741E-5
34-35	3.489072225788829E-4	0.0	0.0	0.31801231875100516	9.69186729385786E-5
36-37	3.6829095716659865E-4	0.0	0.0	0.5257118039633202	1.0522598776188533E-4
38-39	3.765982719899054E-4	0.0	0.0	0.8829651088346853	1.3014793223180553E-4
40-41	3.8213648187210986E-4	0.0	0.0	1.3586447247221702	1.5506987670172576E-4
42-43	4.125966362242346E-4	0.0	0.0	1.742594970330702	1.5506987670172576E-4
44-45	4.264421609297458E-4	0.0	0.0	2.1497447771357887	1.5506987670172576E-4
46-47	4.319803708119503E-4	0.0	0.0	2.591131797432663	1.5506987670172576E-4
48-49	4.319803708119503E-4	0.0	0.0	3.0636823247367024	1.5506987670172576E-4
50-51	4.375185806941548E-4	0.0	0.0	3.5844152780159204	1.5506987670172576E-4
52-53	4.4028768563525705E-4	0.0	0.0	4.097807334096277	1.5506987670172576E-4
54-55	4.430567905763593E-4	0.0	0.0	4.564675658061175	1.57838981642828E-4
56-57	4.5413321034076825E-4	0.0	0.0	5.082514896676941	1.6614629646613473E-4
58-59	4.5413321034076825E-4	0.0	0.0	5.669207929653215	1.7722271623054372E-4
60-61	4.6797873504627946E-4	0.0	0.0	6.275849594625189	1.7722271623054372E-4
62-63	4.707478399873817E-4	0.0	0.0	6.889873693370142	1.827609261127482E-4
64-65	4.73516944928484E-4	0.0	0.0	7.489365529384191	1.882991359949527E-4
66-67	4.7628604986958624E-4	0.0	0.0	8.109035833104052	1.882991359949527E-4
68-69	4.929006795161997E-4	0.0	0.0	8.827438573498913	1.938373458771572E-4
70-71	4.929006795161997E-4	0.0	0.0	9.66875357491448	2.0768287058266842E-4
72-73	5.205917289272221E-4	0.0	0.0	10.447536648550074	2.1599018540597516E-4
74-75	5.288990437505289E-4	0.0	0.0	11.184711151340668	2.1599018540597516E-4
76-77	5.316681486916312E-4	0.0	0.0	11.961359245066674	2.1599018540597516E-4
78-79	5.316681486916312E-4	0.0	0.0	12.744085524138399	2.2152839528817965E-4
80-81	5.316681486916312E-4	2.7691049411022457E-6	0.0	13.623780320037643	2.2152839528817965E-4
82-83	5.316681486916312E-4	1.3845524705511228E-5	0.0	14.585573539230685	2.242975002292819E-4
84-85	5.344372536327333E-4	1.938373458771572E-5	0.0	15.47283076072408	2.2706660517038413E-4
86-87	5.482827783382445E-4	2.7691049411022457E-5	0.0	16.349756451682218	2.2706660517038413E-4
88	5.538209882204491E-4	2.7691049411022457E-5	0.0	17.03168454179288	2.2706660517038413E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACAAG	5175	0.0	32.961884	1
GTACATA	3715	0.0	29.219238	1
CTATTGA	11055	0.0	25.712044	9
AGGGCTA	11445	0.0	24.756725	5
GTACTAG	9445	0.0	24.328909	1
CGTTAAC	3155	0.0	24.2775	1
TATTCTC	6405	0.0	24.209538	5
GTATAAG	10060	0.0	23.308714	1
GTGATCG	7665	0.0	23.230953	8
TTTAGGG	12960	0.0	22.552273	2
TATTCGC	1595	0.0	22.386845	9
GGGCTAT	13510	0.0	22.361225	6
ATTTAGG	13230	0.0	22.305683	1
GGCTATT	13570	0.0	21.535278	7
TACATGA	5390	0.0	21.184706	2
TGATCGC	8495	0.0	20.961184	9
TAGGCAT	13785	0.0	20.86106	5
GTACAAA	5315	0.0	20.776882	1
ATAAGGT	9270	0.0	20.580164	3
ACGTTAA	2140	0.0	20.41781	8
>>END_MODULE