FastQCFastQC Report
Fri 27 May 2016
ERR765459_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765459_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19654176
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA232150.1181173914388474No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT225490.11472879860239371No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA604700.023.8096451
GTACTAG57200.022.0983751
CAACGCA678100.020.3792295
ATCAACG682350.020.1634923
AACGCAG708950.019.7221896
GTACATG1009050.019.6239281
TACATGG1010100.019.2359732
TCAACGC725400.019.01814
CATGGGG423500.018.7952734
ACATGGG978350.018.5105513
ACGCAGA751000.018.3176167
TATCAAC793550.018.2380922
CGCAGAG758550.018.0547778
TATATAC107250.017.612893
GCAGAGT810550.016.3806139
TATACCG33850.015.96340755
GTTATAT63450.015.9224871
GTATAAG78600.015.9024191
GTATAAT54400.015.6344921
GTATTAT53350.015.4137311
Produced by FastQC (version 0.11.5)