FastQCFastQC Report
Fri 27 May 2016
ERR765458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14108048
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT247420.17537507669381336No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC169260.11997407437230154No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA165230.11711754879200865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT160300.11362308945929302No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141450.10026192142243917No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA502350.027.9107171
TATTCGC11950.025.9476289
GTACAAG60500.023.9207021
CAACGCA544400.023.5786865
ATCAACG551300.023.3270193
AACGCAG592650.022.283546
TATCAAC633350.022.0559162
GTACTAG74100.021.6863521
GTACATG819950.021.0767171
GTATAAG74300.020.9955791
GTGATCG53350.020.958698
ACGCAGA610400.020.7581217
CGCAGAG606900.020.7230118
TCAACGC627150.020.5649644
TACATGG805300.020.4621892
CTATTGA62850.019.9584879
GCAGAGT612000.019.6828619
TAGTACT95500.019.6794824
TGATCGC58200.019.2121339
CATGGGG373950.019.1859174
Produced by FastQC (version 0.11.5)