FastQCFastQC Report
Fri 27 May 2016
ERR765456_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765456_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5507564
Sequences flagged as poor quality0
Sequence length99
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT241450.43839708444604547No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA241190.43792500640936716No Hit
GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC95660.17368840380247963No Hit
GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC90540.16439209784943035No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG90220.16381107872736478No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT82950.15061105054793733No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG82350.14952163969406437No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG71980.13069298876962665No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC67000.12165087868248105No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC65350.1186549988343304No Hit
CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC64120.11642170658389081No Hit
GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT62550.11357108151625656No Hit
GTATAAGGTGATCGCAGGTTGTGCAATCATTGCTCAAAAGGGTGTACACC60070.1090681833202483No Hit
CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA57110.10369375644114168No Hit
GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA56970.103439560575238No Hit
CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA55320.10044368072708733No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACATGA17300.048.6479872
GTACATA14650.045.3869321
GTACAAG39050.035.48361
GTACAAA39200.033.449951
TACAAGA25650.030.635982
GTACTAG35150.030.2930221
GTAACAA16000.029.0611271
TACATAA13800.027.966072
TATTCGC6400.027.6075679
TAGGCAT51050.026.9595495
CGTTAAC11500.026.2813661
ACGTTAA9200.026.2799348
TACAAGG27250.026.1070252
GTGATCG29300.024.7551618
GGTAACA6750.024.1099721
GTATAAG36850.024.1006261
CTAGGCA57500.024.0971164
GACGTTA9850.023.6016627
GGAAACA31750.023.2855131
ATAAGGT31450.023.2119393
Produced by FastQC (version 0.11.5)