##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 5507564 Sequences flagged as poor quality 0 Sequence length 99 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.26796020890543 33.0 33.0 33.0 33.0 33.0 2 32.097133505847594 33.0 33.0 33.0 27.0 33.0 3 32.12758199450791 33.0 33.0 33.0 27.0 33.0 4 36.068252134700565 37.0 37.0 37.0 33.0 37.0 5 36.09268398878343 37.0 37.0 37.0 33.0 37.0 6 36.13124059929218 37.0 37.0 37.0 33.0 37.0 7 36.02912812270543 37.0 37.0 37.0 33.0 37.0 8 35.99574748473191 37.0 37.0 37.0 33.0 37.0 9 35.698328335358426 37.0 37.0 37.0 33.0 37.0 10-11 35.88411010748128 37.0 37.0 37.0 33.0 37.0 12-13 35.80297423688586 37.0 37.0 37.0 33.0 37.0 14-15 37.07069886069413 37.0 37.0 40.0 33.0 40.0 16-17 37.260385353669975 37.0 37.0 40.0 33.0 40.0 18-19 37.41075164991274 37.0 37.0 40.0 33.0 40.0 20-21 37.44947575734027 37.0 37.0 40.0 33.0 40.0 22-23 37.36574826910772 37.0 37.0 40.0 33.0 40.0 24-25 37.28873409369369 37.0 37.0 40.0 33.0 40.0 26-27 37.115069021440334 37.0 37.0 40.0 33.0 40.0 28-29 37.015785563272615 37.0 37.0 40.0 33.0 40.0 30-31 36.79911318325125 37.0 37.0 40.0 33.0 40.0 32-33 36.52012096454985 37.0 37.0 40.0 33.0 40.0 34-35 36.34977641657909 37.0 37.0 40.0 33.0 40.0 36-37 36.104481854409684 37.0 37.0 40.0 33.0 40.0 38-39 35.86857383772572 37.0 37.0 40.0 33.0 40.0 40-41 35.60894526146224 37.0 37.0 40.0 27.0 40.0 42-43 35.281867173944775 37.0 35.0 40.0 27.0 40.0 44-45 34.85275876957581 37.0 33.0 40.0 24.5 40.0 46-47 34.29767652268771 37.0 33.0 40.0 22.0 40.0 48-49 33.76885316266865 37.0 33.0 40.0 18.5 40.0 50-51 33.32446050558832 37.0 33.0 40.0 15.0 40.0 52-53 33.13315896102161 37.0 33.0 40.0 15.0 40.0 54-55 33.016418510978724 37.0 33.0 40.0 15.0 40.0 56-57 32.837154411641876 37.0 33.0 37.0 15.0 40.0 58-59 32.632364145019466 37.0 33.0 37.0 15.0 40.0 60-61 32.453836215067135 37.0 33.0 37.0 15.0 40.0 62-63 32.123536648870534 37.0 33.0 37.0 15.0 40.0 64-65 31.978625305125824 37.0 33.0 37.0 15.0 40.0 66-67 31.811007824875027 37.0 33.0 37.0 15.0 40.0 68-69 31.58279095803517 37.0 33.0 37.0 15.0 37.0 70-71 31.431467795925748 35.0 33.0 37.0 15.0 37.0 72-73 31.257460285527323 33.0 33.0 37.0 15.0 37.0 74-75 31.080775457171264 33.0 33.0 37.0 15.0 37.0 76-77 30.947235111566563 33.0 33.0 37.0 15.0 37.0 78-79 30.776010228841642 33.0 27.0 37.0 15.0 37.0 80-81 30.674058712708558 33.0 27.0 37.0 15.0 37.0 82-83 30.53111039653829 33.0 27.0 37.0 15.0 37.0 84-85 30.385302921582028 33.0 27.0 37.0 15.0 37.0 86-87 30.298775647455024 33.0 27.0 37.0 15.0 37.0 88-89 30.211231771432885 33.0 27.0 37.0 15.0 37.0 90-91 30.143743676877833 33.0 27.0 37.0 15.0 37.0 92-93 30.030601006179864 33.0 27.0 37.0 15.0 37.0 94-95 29.977008982555624 33.0 27.0 37.0 15.0 37.0 96-97 29.932095841283008 33.0 27.0 37.0 15.0 37.0 98-99 29.127322260803506 33.0 27.0 35.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19419372838069648 1101 2 0.35225408110136414 1101 3 0.29346805359611494 1101 4 0.23584518728280557 1101 5 0.14702117805768466 1101 6 0.20428241352038867 1101 7 0.19025726520764863 1101 8 0.13244055303605506 1101 9 0.3922337772055471 1101 10-11 0.31245978785578643 1101 12-13 0.3741137022355545 1101 14-15 0.6368996355181409 1101 16-17 0.5336632926309122 1101 18-19 0.3488125096175665 1101 20-21 0.42254755639119423 1101 22-23 0.29233496601101905 1101 24-25 0.43008585638688146 1101 26-27 0.2983971997382895 1101 28-29 0.2727559905289212 1101 30-31 0.33272605027525515 1101 32-33 0.4378590850476698 1101 34-35 0.5317128922671372 1101 36-37 0.516998827218103 1101 38-39 0.4261187489020202 1101 40-41 0.379894959674985 1101 42-43 0.4694850724464743 1101 44-45 0.48925952817488394 1101 46-47 0.5563848158322955 1101 48-49 0.6004725595841052 1101 50-51 0.6709771365853356 1101 52-53 0.6723830982182903 1101 54-55 0.6394339466998957 1101 56-57 0.6205258232458633 1101 58-59 0.5978316541207462 1101 60-61 0.4998969630704124 1101 62-63 0.6109026593913782 1101 64-65 0.5414771748970857 1101 66-67 0.5290086105954863 1101 68-69 0.37079845113838417 1101 70-71 0.4063661977367481 1101 72-73 0.5869939297947546 1101 74-75 0.47154202393709 1101 76-77 0.49541938036411537 1101 78-79 0.6537553470417663 1101 80-81 0.5843230244292492 1101 82-83 0.5918613648778397 1101 84-85 0.6673172193130519 1101 86-87 0.45330531982400046 1101 88-89 0.5671580716790565 1101 90-91 0.5321163374548306 1101 92-93 0.6737004418451384 1101 94-95 0.6698577838812305 1101 96-97 0.5190609331794214 1101 98-99 0.6669617307592901 1102 1 0.12588209509974035 1102 2 0.36285581640406406 1102 3 0.2561970566819056 1102 4 0.20120173145862452 1102 5 0.13593497768277985 1102 6 0.21486307504174817 1102 7 0.2009227871070962 1102 8 0.22260342205948547 1102 9 0.4449312893369566 1102 10-11 0.3304872265568122 1102 12-13 0.34297499451982816 1102 14-15 0.5829373414118066 1102 16-17 0.44983084113531646 1102 18-19 0.30160194923556816 1102 20-21 0.4113020562015137 1102 22-23 0.24935817033136232 1102 24-25 0.3670500457131496 1102 26-27 0.3461966665049516 1102 28-29 0.34859315229002164 1102 30-31 0.32988100283272104 1102 32-33 0.40286218683579733 1102 34-35 0.46149225557400086 1102 36-37 0.4246669095384945 1102 38-39 0.3452871371980635 1102 40-41 0.42651059880349607 1102 42-43 0.3697704468103069 1102 44-45 0.43837818031786213 1102 46-47 0.5530516436684678 1102 48-49 0.571815115696964 1102 50-51 0.5215608600372548 1102 52-53 0.6272057664065613 1102 54-55 0.5071256093351479 1102 56-57 0.6075363154670725 1102 58-59 0.5614077174125853 1102 60-61 0.4479765719897273 1102 62-63 0.5840199061711218 1102 64-65 0.4770534368680224 1102 66-67 0.46700240052605935 1102 68-69 0.4497170301927369 1102 70-71 0.436844472856734 1102 72-73 0.5261626772436863 1102 74-75 0.407774928411726 1102 76-77 0.437815155659397 1102 78-79 0.6192848947876968 1102 80-81 0.5693326596693424 1102 82-83 0.5481598572212576 1102 84-85 0.5020660034616746 1102 86-87 0.4968793919768615 1102 88-89 0.530124857747392 1102 90-91 0.4982499736940582 1102 92-93 0.49294787276422625 1102 94-95 0.5430477468015198 1102 96-97 0.5076593217801246 1102 98-99 0.4058531086926891 1103 1 0.15085965183843086 1103 2 0.30316719271563386 1103 3 0.2329340485343394 1103 4 0.21382235842511932 1103 5 0.0996710432069392 1103 6 0.1743848953241809 1103 7 0.18661368265710365 1103 8 0.10717945043403887 1103 9 0.382004852297797 1103 10-11 0.255046040535035 1103 12-13 0.30241407923330144 1103 14-15 0.5439355530142151 1103 16-17 0.41497199005457475 1103 18-19 0.2307040844076056 1103 20-21 0.33301056307185917 1103 22-23 0.20142622361726126 1103 24-25 0.3417995175865869 1103 26-27 0.2285161515052465 1103 28-29 0.11793273151037909 1103 30-31 0.15322240232174522 1103 32-33 0.26896617506636034 1103 34-35 0.334638328487074 1103 36-37 0.41294519024427956 1103 38-39 0.23787328368909755 1103 40-41 0.16018019391402305 1103 42-43 0.2856510561828287 1103 44-45 0.3859576387658237 1103 46-47 0.4546940401821047 1103 48-49 0.4053286545552339 1103 50-51 0.316173305844238 1103 52-53 0.3988611088904648 1103 54-55 0.3712868406448493 1103 56-57 0.49439418308131877 1103 58-59 0.4641424445241782 1103 60-61 0.37496142025444357 1103 62-63 0.4063537922999956 1103 64-65 0.2225063543139747 1103 66-67 0.30992793943280006 1103 68-69 0.3666381437873625 1103 70-71 0.34146059790571215 1103 72-73 0.39920743748798415 1103 74-75 0.32121616646086437 1103 76-77 0.2971466281434978 1103 78-79 0.49998336273111477 1103 80-81 0.32271146660377426 1103 82-83 0.35441832135852636 1103 84-85 0.44024435832080044 1103 86-87 0.23957162892556028 1103 88-89 0.3593224654303988 1103 90-91 0.1725290975187832 1103 92-93 0.10149386699309204 1103 94-95 0.37496842137937136 1103 96-97 0.33170983816894406 1103 98-99 0.2622239886896196 1104 1 0.13891555251291265 1104 2 0.3536227191827592 1104 3 0.2601069280224735 1104 4 0.20618443249276197 1104 5 0.09476761414190094 1104 6 0.137555654754685 1104 7 0.1760093943996921 1104 8 0.1248730722465794 1104 9 0.40237830751988923 1104 10-11 0.2676063256244774 1104 12-13 0.2992668528368654 1104 14-15 0.5184423529877975 1104 16-17 0.35491854775677467 1104 18-19 0.26686963792194973 1104 20-21 0.39498826894470085 1104 22-23 0.28370350242215636 1104 24-25 0.369426465755744 1104 26-27 0.19877991360469593 1104 28-29 0.236802934601819 1104 30-31 0.2333651864847468 1104 32-33 0.36197416810053795 1104 34-35 0.4272328243656318 1104 36-37 0.31918721907688763 1104 38-39 0.10963894576976685 1104 40-41 0.07989968769744848 1104 42-43 0.12857758221375093 1104 44-45 0.14319892715577964 1104 46-47 0.24785138938891293 1104 48-49 0.38500450349027915 1104 50-51 0.23123459553845294 1104 52-53 0.33825963056858654 1104 54-55 0.19006313273278863 1104 56-57 0.34228040731248655 1104 58-59 0.3876309492117045 1104 60-61 0.24869481892288547 1104 62-63 0.2879078190502966 1104 64-65 0.1775458434155759 1104 66-67 0.2617292348764657 1104 68-69 0.18666354025236842 1104 70-71 0.21679184986791 1104 72-73 0.2619524529510784 1104 74-75 0.23423221041811715 1104 76-77 0.20217924381882213 1104 78-79 0.35369126634032355 1104 80-81 0.300870598253411 1104 82-83 0.3055998534762274 1104 84-85 0.20345318216629593 1104 86-87 0.22213315489380037 1104 88-89 0.20807704425204676 1104 90-91 0.18409529924792878 1104 92-93 0.22687993030439202 1104 94-95 0.383793576767399 1104 96-97 0.32389356936050717 1104 98-99 0.20465167017448138 1105 1 0.15145354703358294 1105 2 0.31940733190899095 1105 3 0.2710208830760621 1105 4 0.1871901420725024 1105 5 0.03866422612377818 1105 6 0.13399258457726404 1105 7 0.1321270435598123 1105 8 0.01855256180292031 1105 9 0.36941196090739936 1105 10-11 0.24863493610602205 1105 12-13 0.24171058674721024 1105 14-15 0.4250825068530517 1105 16-17 0.31233081784951366 1105 18-19 0.24202533705504692 1105 20-21 0.3105423011579873 1105 22-23 0.19904604743787502 1105 24-25 0.3064288747810835 1105 26-27 0.15075468024464556 1105 28-29 0.19261257078623828 1105 30-31 0.20719004415370534 1105 32-33 0.2752271442831855 1105 34-35 0.33365263666406975 1105 36-37 0.2714466270345781 1105 38-39 0.18211025491352473 1105 40-41 0.09608184706418399 1105 42-43 0.28012175928562044 1105 44-45 0.2847186429076842 1105 46-47 0.3760704579757288 1105 48-49 0.3351743356920238 1105 50-51 0.2766506283573591 1105 52-53 0.15072640245386992 1105 54-55 0.29844714042660314 1105 56-57 0.3802353013971995 1105 58-59 0.3250817050037682 1105 60-61 0.302893181439174 1105 62-63 0.34245429188912624 1105 64-65 0.2072242214335951 1105 66-67 0.276834380338034 1105 68-69 0.1743331630368523 1105 70-71 0.26371148473283057 1105 72-73 0.22062416187756995 1105 74-75 0.19925284130611587 1105 76-77 0.147807310743179 1105 78-79 0.37681724497657854 1105 80-81 0.30944348992701975 1105 82-83 0.25628711590879405 1105 84-85 0.23830168107033245 1105 86-87 0.2621419511108023 1105 88-89 0.1537045296541102 1105 90-91 0.09838971955534959 1105 92-93 0.04742181770146203 1105 94-95 0.31551453800159734 1105 96-97 0.26568034922649275 1105 98-99 0.1970151955454007 1106 1 0.1297286134489255 1106 2 0.25595055281452517 1106 3 0.2639355287573082 1106 4 0.21650754776074876 1106 5 0.05454450582956838 1106 6 0.1717639288089714 1106 7 0.12152317034655624 1106 8 -0.005337451666989068 1106 9 0.4016696510414519 1106 10-11 0.20259906455347476 1106 12-13 0.21572966927969617 1106 14-15 0.40484258633690473 1106 16-17 0.30895307812256334 1106 18-19 0.21929987267448325 1106 20-21 0.3352509793719278 1106 22-23 0.14287092056788708 1106 24-25 0.2202713108736205 1106 26-27 0.04381151535446293 1106 28-29 0.13654972977538904 1106 30-31 0.06972172495380846 1106 32-33 0.14336418730168532 1106 34-35 0.21488074297931803 1106 36-37 0.11375644085433834 1106 38-39 0.05971006695920522 1106 40-41 0.06229646563221536 1106 42-43 0.13249004022191002 1106 44-45 0.13453097667727576 1106 46-47 0.3409950306872247 1106 48-49 0.41966962989249623 1106 50-51 0.3155242534420495 1106 52-53 0.33962912171693915 1106 54-55 0.34722972849918676 1106 56-57 0.3576743766127848 1106 58-59 0.2519438682333046 1106 60-61 0.28146563732970264 1106 62-63 0.3356723220266744 1106 64-65 0.1602863452965444 1106 66-67 0.25910187096295445 1106 68-69 0.2615830207349319 1106 70-71 0.17022371885679277 1106 72-73 0.2681095979146342 1106 74-75 0.21286037839940875 1106 76-77 0.18268515058768386 1106 78-79 0.3203021875869112 1106 80-81 0.2842398759493676 1106 82-83 0.2501907962894343 1106 84-85 0.13960767878966251 1106 86-87 0.22744512677165218 1106 88-89 0.11887924535344752 1106 90-91 0.1720235346949366 1106 92-93 0.052949327476298436 1106 94-95 0.29056708942989573 1106 96-97 0.23997278643571462 1106 98-99 0.20713237780879368 1107 1 0.12534621141117697 1107 2 0.21002787638119713 1107 3 0.17826762426795284 1107 4 0.1646737571461543 1107 5 0.021485036016500203 1107 6 0.11339886076002159 1107 7 0.08714245167613655 1107 8 0.04633562096125843 1107 9 0.34089298157363856 1107 10-11 0.22828033538179682 1107 12-13 0.1584556043365879 1107 14-15 0.27155916160320714 1107 16-17 0.19137007168217934 1107 18-19 0.04502769430997944 1107 20-21 0.18427413673860826 1107 22-23 0.12002286425354214 1107 24-25 0.18498944073971302 1107 26-27 0.032473057251863224 1107 28-29 0.020820493038279153 1107 30-31 0.020597075962712097 1107 32-33 0.0214314066404242 1107 34-35 0.1713898214262457 1107 36-37 0.16745623408854016 1107 38-39 0.11146843466967482 1107 40-41 0.015932760373061683 1107 42-43 0.0319741987964548 1107 44-45 0.08579874676551924 1107 46-47 0.16484147807797456 1107 48-49 0.23884091017047382 1107 50-51 0.06566300717345541 1107 52-53 0.056139621972135956 1107 54-55 0.13057800646807038 1107 56-57 0.1343532538403096 1107 58-59 0.13913328193189045 1107 60-61 0.13755803877055683 1107 62-63 0.21357681138624685 1107 64-65 0.02688958500737826 1107 66-67 0.01887185994393903 1107 68-69 0.037004301851240484 1107 70-71 0.07066111938889463 1107 72-73 0.1105751073277439 1107 74-75 0.1646686837716338 1107 76-77 0.20427072164825333 1107 78-79 0.19569204137539487 1107 80-81 0.16487394207284467 1107 82-83 0.061332287001743424 1107 84-85 0.03076924248365387 1107 86-87 0.1383038385999562 1107 88-89 0.059089277283771224 1107 90-91 0.024714481196717486 1107 92-93 -0.0321972026073567 1107 94-95 0.27479470886011015 1107 96-97 0.12730226836768566 1107 98-99 -0.004456311840641547 1108 1 0.11679559787598492 1108 2 0.2844703899880585 1108 3 0.14814544936510998 1108 4 0.12777577244171567 1108 5 0.009843382473064821 1108 6 0.09740560557295197 1108 7 0.14151854816554277 1108 8 0.004268009865569411 1108 9 0.31485826973313635 1108 10-11 0.21479476705314937 1108 12-13 0.22771056524959477 1108 14-15 0.2425142277792034 1108 16-17 0.2632693451514143 1108 18-19 9.457854081915684E-4 1108 20-21 0.18753965055666555 1108 22-23 0.07724834487966348 1108 24-25 0.07826325254014677 1108 26-27 -0.010006725770026037 1108 28-29 0.022588312498129426 1108 30-31 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-0.44304286844659657 2216 6 -0.3891650859322482 2216 7 -0.42797088335211697 2216 8 -0.41546034047969727 2216 9 -0.05697844470497415 2216 10-11 -0.31297791139441955 2216 12-13 -0.2649281056439108 2216 14-15 -0.7943718701368496 2216 16-17 -0.8602621686144971 2216 18-19 -0.8548316577650539 2216 20-21 -1.155803690103589 2216 22-23 -1.0448235699433965 2216 24-25 -0.8218226982308039 2216 26-27 -1.0152759794110437 2216 28-29 -1.0793785090396568 2216 30-31 -0.7468740168230141 2216 32-33 -0.8037292465965749 2216 34-35 -0.7767679286614566 2216 36-37 -0.8346441390314752 2216 38-39 -0.8495346431798936 2216 40-41 -0.8657498589379884 2216 42-43 -0.9499777359025785 2216 44-45 -0.7859674730187933 2216 46-47 -1.1384539940601712 2216 48-49 -1.2544082308865043 2216 50-51 -0.9266902765525771 2216 52-53 -0.8425544721643305 2216 54-55 -0.7080545093721327 2216 56-57 -0.8040058986787102 2216 58-59 -0.771741980651143 2216 60-61 -0.8510968614301362 2216 62-63 -0.9485196274267516 2216 64-65 -0.917856545665586 2216 66-67 -0.8487124826504022 2216 68-69 -0.8967852311877067 2216 70-71 -0.9115873157653418 2216 72-73 -0.7202453732258469 2216 74-75 -0.761135370695083 2216 76-77 -0.7239241202906079 2216 78-79 -0.6798557647158212 2216 80-81 -0.7255012090144888 2216 82-83 -0.6311636118027657 2216 84-85 -0.7252364913782294 2216 86-87 -0.8703030758380983 2216 88-89 -0.7372773617811994 2216 90-91 -0.7606432495745317 2216 92-93 -0.6917686827163791 2216 94-95 -0.7375323485196965 2216 96-97 -0.8323934832909003 2216 98-99 -0.8262497237938362 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 7.0 9 28.0 10 106.0 11 217.0 12 382.0 13 855.0 14 2322.0 15 6440.0 16 14640.0 17 28062.0 18 46672.0 19 65956.0 20 85396.0 21 108161.0 22 124225.0 23 105666.0 24 68973.0 25 48571.0 26 49224.0 27 62071.0 28 80732.0 29 105420.0 30 139499.0 31 184638.0 32 242802.0 33 318977.0 34 420027.0 35 585116.0 36 942761.0 37 1273990.0 38 395211.0 39 417.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.59827858620758 20.716989900314818 14.16381244993804 22.52091906353956 2 15.298304740045612 22.378738796321855 38.49910661107802 23.82384985255451 3 17.00020190414492 29.49823188618416 31.278547103583364 22.223019106087555 4 12.135927971059438 18.378542673312555 38.13773203543345 31.347797320194555 5 11.75900236770623 38.94751463474863 33.48423223857182 15.809250758973317 6 27.565380992395188 40.397424342231886 17.144730410758733 14.892464254614199 7 22.845544781685696 35.066446799347226 23.54080678862742 18.547201630339657 8 27.043898173493762 31.7907517733793 20.02576819806361 21.139581855063327 9 27.18316766693546 12.849494121767755 21.507083348388964 38.46025486290783 10-11 24.784623474189317 26.060459760431293 26.52236996247343 22.632546802905967 12-13 27.0329677512599 24.692377610137623 27.272601825416825 21.00205281318565 14-15 24.08158833444895 23.718024897095823 28.08541625453399 24.114970513921243 16-17 20.607668215329713 26.200658721983476 28.81213241041804 24.379540652268766 18-19 21.0820791188264 23.721040735976924 34.5398437494326 20.65703639576408 20-21 23.214437453654647 22.849448503912072 33.53918356645515 20.396930475978127 22-23 22.537395843243946 22.530804907578013 33.206831550209856 21.724967698968182 24-25 21.50099390583568 23.44161593038229 33.42021082278844 21.637179340993587 26-27 21.924693022178225 23.068274830760025 33.35194107594573 21.655091071116015 28-29 20.97368273886604 23.02028628264692 34.25516253646803 21.750868442019012 30-31 21.679602815328156 23.04172044119687 33.79970709373509 21.478969649739884 32-33 21.516000540347783 23.39124883523823 32.912427345374475 22.18032327903952 34-35 21.72368764121488 23.60948506454033 33.47711438305574 21.189712911189048 36-37 22.15352377203424 22.9005509513825 32.68351488970441 22.262410386878845 38-39 22.370552570973302 22.544286366894692 32.747817365354265 22.33734369677774 40-41 21.934915327357068 22.8220861346323 33.05893494837282 22.184063589637816 42-43 22.639355620742673 23.074293825727672 31.854391524093046 22.43195902943661 44-45 22.322423230478286 23.735567627691527 30.896477293267292 23.04553184856289 46-47 22.30601405630511 23.922763312419065 30.04016839386705 23.73105423740877 48-49 23.78688654366976 24.329158953032593 28.382983838226846 23.5009706650708 50-51 22.70781601448481 25.244000795996197 27.39761172089875 24.650571468620246 52-53 22.368275080947868 25.53967212686133 26.98525861829483 25.106794173895974 54-55 22.602805886595235 25.746246434902982 26.87082710250848 24.780120575993305 56-57 23.292494027712287 24.91224273134864 26.507052810148156 25.288210430790915 58-59 22.07672938525998 25.100107779047143 27.95376504022468 24.869397795468196 60-61 22.807823930870345 25.41343141904479 27.13261253069415 24.646132119390714 62-63 22.386675851610622 25.002133429588834 26.808131507868087 25.803059210932457 64-65 21.590543478024042 24.97078563226864 27.306555130362536 26.13211575934479 66-67 23.134166030571773 24.454050829005347 27.148227419599664 25.263555720823216 68-69 22.597426720415335 23.907135116379337 27.450630600843162 26.04480756236217 70-71 21.91045754422788 24.327564590821154 27.326644944989205 26.435332919961763 72-73 22.8193716859214 24.18766263996206 27.485173118278787 25.507792555837757 74-75 22.929402182162566 24.323984251476695 27.29213859339628 25.45447497296445 76-77 22.219968755696712 24.590100087806515 27.246083749548806 25.943847406947974 78-79 22.412768959518885 25.01689727915903 26.84806756406009 25.722266197261987 80-81 21.854401192405046 24.787052694908574 27.264154703260967 26.094391409425416 82-83 22.15095457810386 24.196822769558374 27.216996479750392 26.435226172587374 84-85 22.21345952584482 24.61630041884216 26.28187343805719 26.888366617255834 86-87 21.666983806270796 24.32778811104147 26.911480284205503 27.09374779848223 88-89 22.10854018219307 23.618236665066444 26.978814953398633 27.294408199341852 90-91 22.22127705468931 23.943159460435027 26.54791206540551 27.287651419470155 92-93 21.96694102782894 23.822104008127795 26.702446826580704 27.508508137462563 94-95 20.874985292900067 23.87912833742227 27.60953884344819 27.63634752622948 96-97 21.96529110354957 23.846762730546004 26.696650353001754 27.491295812902667 98-99 21.551678745812122 23.928074190331696 26.66601786198036 27.854229201875818 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 113.0 1 91.5 2 167.5 3 265.0 4 203.5 5 183.0 6 340.0 7 881.0 8 1618.0 9 1663.5 10 1106.0 11 809.5 12 890.5 13 1108.5 14 1495.5 15 2212.5 16 3018.5 17 3951.5 18 5324.0 19 6962.0 20 8962.5 21 11545.5 22 14559.0 23 17992.0 24 21857.0 25 26308.0 26 31035.5 27 35272.5 28 41232.5 29 50064.0 30 57152.0 31 65281.5 32 75023.0 33 83126.5 34 90796.5 35 98248.0 36 101059.5 37 109365.0 38 127150.0 39 124862.5 40 115494.0 41 120938.0 42 130028.0 43 145937.5 44 171682.0 45 208505.5 46 236686.5 47 257641.0 48 268132.0 49 278244.5 50 301250.0 51 282791.0 52 249511.0 53 233810.5 54 202664.5 55 186091.0 56 168381.5 57 135012.5 58 127119.0 59 111833.5 60 83964.5 61 62449.5 62 49028.5 63 39036.5 64 26590.5 65 18714.5 66 12798.0 67 9158.0 68 7234.5 69 6341.0 70 6257.0 71 6094.5 72 6894.0 73 6158.0 74 3780.0 75 2833.0 76 2223.0 77 1392.5 78 602.5 79 320.0 80 212.0 81 150.0 82 122.5 83 84.5 84 50.0 85 38.5 86 23.0 87 8.5 88 3.5 89 3.5 90 2.0 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.056921717114862394 2 0.008061640318659937 3 0.0 4 0.0 5 0.0022514490980041265 6 0.0 7 0.0 8 0.0 9 0.003831094836119925 10-11 0.0 12-13 0.0 14-15 0.0024420959974318954 16-17 0.004230545482540012 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.0 44-45 4.2668591776691113E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 1.2709793295184586E-4 54-55 0.0 56-57 7.807444452756245E-4 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0014253125338171287 70-71 1.6341162808094467E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 3.722153750732629E-4 80-81 7.171954787997017E-4 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 2.9050956103279053E-4 92-93 7.716660214933498E-4 94-95 3.631369512909882E-4 96-97 2.4511744212141705E-4 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 5507564.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 21.523206905799828 #Duplication Level Percentage of deduplicated Percentage of total 1 70.87659357235701 15.25491588236122 2 13.913889034314828 5.989430250997948 3 5.580692048645788 3.603431689216656 4 2.65617800063144 2.2867787474489676 5 1.5270315865795807 1.6433308394822048 6 0.9916669829892757 1.2806312193917075 7 0.6197082727666358 0.9336676563194497 8 0.45924558238563223 0.7907550152208404 9 0.354487911698968 0.6866745002191609 >10 2.2061084287035695 9.427243118296767 >50 0.3210032645609889 4.890363481675278 >100 0.3695481496800354 17.36963212021829 >500 0.07058512726416444 10.706946017230287 >1k 0.050331763518882536 19.359033350068348 >5k 0.0024993512706294406 3.5541731826670184 >10k+ 4.3092263286714495E-4 2.2229929291859496 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 31552 0.5728848543566629 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 27011 0.49043460956604407 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 23115 0.41969553145455957 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22722 0.41255989036169166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15440 0.2803417263966429 No Hit GCCTAGTACTGTGCGCCAATTAGGTCGTCATTGCGCCAGCTCGTCAGCGC 9538 0.17318001207067224 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9192 0.16689774281333816 No Hit GTATAAAATCAGGCAGTTTTTGATCACGTTTATTGTAAGCCGTCAGCATC 9049 0.1643013136116076 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7791 0.14145999937540443 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 7713 0.1400437652653696 No Hit GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCTTGGATAT 7708 0.13995298102754686 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 7651 0.1389180407163675 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCC 7650 0.13889988386880298 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTC 7542 0.13693894433183165 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 7115 0.12918597042176905 No Hit CGTTTATTGTAAGCCGTCAGCATCGGGATATCATCTGCTTCAAGCTCCTC 7020 0.12746106990313685 No Hit GACCATAATGTGATCCCTTCCGGCGGTCGGTATAAAATCAGGCAGTTTTT 6945 0.12609930633579564 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG 6784 0.1231760538779032 No Hit GTTAATAATTCTGGCAATTCGTCTCCACACTAGAAGTCGACGAACAACGA 6533 0.11861868513920129 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTCCC 6411 0.11640354973632627 No Hit CCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAACTTTTCAGGCGAA 6224 0.11300821924175551 No Hit CAATTGATATTAGATATTTTTGCAAAGCGGGCGAGAACGAGAGAAGGCAA 6180 0.11220931794891534 No Hit ATCTAATTCACCTCCTATTAGGAGCCGATCGTGCTTGTGCGCCGGCAAAA 5694 0.10338509003254433 No Hit ATTTAGGGCTATTGATATTTAACAAATATCCAGCAAAGGTTTTTCCAGGA 5663 0.10282222775804331 No Hit ATTATTAACTGCGCAGTTAGGGCAGCGTCTGAGGAAGTTTGCTGCGGTTT 5641 0.10242277711162322 No Hit GTATACGGGCGTCGGCATCCAGACCGTCGGCTGATCGTGGTTTTACTAGG 5548 0.10073419028812011 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG 5529 0.10038921018439369 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.8156847564549408E-5 0.0 2 0.0 0.0 0.0 1.8156847564549408E-5 0.0 3 0.0 0.0 0.0 3.6313695129098816E-5 0.0 4 0.0 0.0 0.0 3.6313695129098816E-5 0.0 5 0.0 0.0 0.0 3.6313695129098816E-5 0.0 6 0.0 0.0 0.0 3.6313695129098816E-5 0.0 7 0.0 0.0 0.0 3.6313695129098816E-5 0.0 8 0.0 0.0 0.0 3.6313695129098816E-5 0.0 9 0.0 0.0 0.0 5.4470542693648224E-5 0.0 10-11 0.0 0.0 0.0 1.1801950916957115E-4 0.0 12-13 1.8156847564549408E-5 0.0 0.0 1.906468994277688E-4 0.0 14-15 3.6313695129098816E-5 0.0 0.0 2.2696059455686762E-4 0.0 16-17 6.354896647592293E-5 0.0 0.0 3.0866640859733997E-4 0.0 18-19 9.078423782274704E-5 0.0 0.0 4.085290702023617E-4 0.0 20-21 1.0894108538729645E-4 0.0 0.0 7.35352326364251E-4 0.0 22-23 1.0894108538729645E-4 0.0 0.0 0.0030685072384088502 0.0 24-25 1.4525478051639527E-4 0.0 0.0 0.008915012154193758 0.0 26-27 2.9958798481506525E-4 0.0 0.0 0.014089713710090341 0.0 28-29 3.0866640859733997E-4 0.0 0.0 0.030285621737668415 0.0 30-31 3.2682325616188935E-4 0.0 0.0 0.0875704758038218 0.0 32-33 3.2682325616188935E-4 0.0 0.0 0.18014316311167697 0.0 34-35 3.2682325616188935E-4 0.0 0.0 0.2988435540649187 0.0 36-37 3.6313695129098816E-4 0.0 0.0 0.5048420681085141 0.0 38-39 3.812937988555376E-4 0.0 0.0 0.8305759134165305 0.0 40-41 3.903722226378123E-4 0.0 0.0 1.2170081001328357 0.0 42-43 4.1760749398463636E-4 0.0 0.0 1.5562234047575298 0.0 44-45 4.6299961289600995E-4 0.0 0.0 1.9471948033649722 0.0 46-47 4.811564604605593E-4 0.0 9.078423782274704E-6 2.3506944267919536 0.0 48-49 4.90234884242834E-4 0.0 1.8156847564549408E-5 2.801093187478166 0.0 50-51 4.90234884242834E-4 0.0 1.8156847564549408E-5 3.343565322164209 0.0 52-53 5.083917318073834E-4 0.0 1.8156847564549408E-5 3.8350984210079084 0.0 54-55 5.083917318073834E-4 0.0 1.8156847564549408E-5 4.273422878063696 0.0 56-57 5.356270031542075E-4 0.0 1.8156847564549408E-5 4.783448726151889 0.0 58-59 5.810191220655811E-4 0.0 1.8156847564549408E-5 5.335988832812474 0.0 60-61 5.991759696301305E-4 0.0 1.8156847564549408E-5 5.875437852379019 0.0 62-63 5.991759696301305E-4 0.0 1.8156847564549408E-5 6.404737194156981 0.0 64-65 5.991759696301305E-4 0.0 1.8156847564549408E-5 6.958539201723303 0.0 66-67 5.991759696301305E-4 0.0 1.8156847564549408E-5 7.540438930895764 0.0 68-69 5.991759696301305E-4 0.0 1.8156847564549408E-5 8.12678708772154 0.0 70-71 6.354896647592294E-4 0.0 1.8156847564549408E-5 8.799489211564314 0.0 72-73 6.627249361060534E-4 0.0 1.8156847564549408E-5 9.450611922076622 0.0 74-75 6.899602074528776E-4 0.0 1.8156847564549408E-5 10.074798949226917 0.0 76-77 7.081170550174269E-4 0.0 1.8156847564549408E-5 10.74087382370863 0.0 78-79 7.081170550174269E-4 0.0 1.8156847564549408E-5 11.399858449216387 0.0 80-81 7.081170550174269E-4 1.8156847564549408E-5 1.8156847564549408E-5 12.102365038336368 0.0 82-83 7.081170550174269E-4 1.8156847564549408E-5 1.8156847564549408E-5 12.853459351539083 0.0 84-85 7.262739025819763E-4 1.8156847564549408E-5 1.8156847564549408E-5 13.568430616512128 0.0 86-87 7.262739025819763E-4 1.8156847564549408E-5 1.8156847564549408E-5 14.283465430451649 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 345 0.0 44.48113 2 TCACGCA 480 0.0 34.876934 4 GTATCAA 67640 0.0 33.526463 1 TCAACGA 700 0.0 27.901546 3 CGCAGCG 1010 0.0 26.70542 8 GTACTAG 3250 0.0 26.341486 1 ATCAACG 86310 0.0 26.136486 3 CAACGCA 86450 0.0 26.067263 5 TATCAAC 87340 0.0 25.913683 2 GGTATCA 43055 0.0 25.848978 1 AACGCAG 91520 0.0 25.14587 6 TTCTGGC 2995 0.0 24.843683 9 TCAACGC 92000 0.0 24.545275 4 AGTACTT 57310 0.0 23.821232 12-13 GAACCGT 595 0.0 23.446041 6 ACGCAGA 95975 0.0 23.251867 7 CGCAGAG 96280 0.0 23.165192 8 TATTCGC 625 0.0 23.066116 9 TAGGCAT 4940 0.0 23.063019 5 AACCGTG 655 0.0 22.718199 7 >>END_MODULE