FastQCFastQC Report
Fri 27 May 2016
ERR765455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6339841
Sequences flagged as poor quality0
Sequence length99
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA243090.3834323289811211No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT231620.3653403926060606No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG97460.1537262527561811No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG71570.11288926646583093No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC70520.11123307351083411No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC69340.10937182809474244No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG65270.10295210873585No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG17850.021.8825471
GTACATA15200.021.7205891
GTACAAG31100.021.23171
ATGGGTA36800.020.0907345
CATGGGT52750.018.6878764
CATGGGG146950.018.226344
TAGTACT25250.017.6789474
GTATAAG21250.017.5060391
CGTTAAC5600.017.4376541
TACATGA21300.016.809612
TCTAACG8950.016.6254542
GTATATA14150.016.4312421
GTACATG579600.016.3585831
ATTGCGT10550.016.3078176
TACATGG565550.016.1314932
GTATTAT13950.016.0001431
TAGGCAT30250.015.9865425
CTAGTAC25450.015.8956383
GTATTAG27800.015.8904291
CTAACGC9400.015.8295543
Produced by FastQC (version 0.11.5)