##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR765455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6339841 Sequences flagged as poor quality 0 Sequence length 99 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.947188423179696 33.0 33.0 33.0 27.0 33.0 2 30.827470594294084 33.0 33.0 33.0 27.0 33.0 3 30.9065323562531 33.0 33.0 33.0 27.0 33.0 4 34.399216163307564 37.0 37.0 37.0 27.0 37.0 5 34.55854791941943 37.0 37.0 37.0 27.0 37.0 6 34.600677840343316 37.0 37.0 37.0 27.0 37.0 7 34.461124498232685 37.0 37.0 37.0 27.0 37.0 8 34.37553859789228 37.0 37.0 37.0 27.0 37.0 9 34.36810386885097 37.0 37.0 37.0 27.0 37.0 10-11 34.31919775275121 37.0 37.0 37.0 27.0 37.0 12-13 34.24889267727692 37.0 37.0 37.0 27.0 37.0 14-15 35.4958547067663 37.0 37.0 40.0 27.0 40.0 16-17 35.525784558319366 37.0 37.0 40.0 27.0 40.0 18-19 35.51910812905245 37.0 37.0 40.0 27.0 40.0 20-21 35.51518145328882 37.0 37.0 40.0 27.0 40.0 22-23 35.55491155062091 37.0 35.0 40.0 27.0 40.0 24-25 35.491749083297194 37.0 33.0 40.0 27.0 40.0 26-27 35.49237685929347 37.0 33.0 40.0 27.0 40.0 28-29 35.3967749348919 37.0 33.0 40.0 27.0 40.0 30-31 35.34769026541833 37.0 33.0 40.0 27.0 40.0 32-33 35.20091119004404 37.0 33.0 40.0 27.0 40.0 34-35 35.14814519796317 37.0 33.0 40.0 27.0 40.0 36-37 35.027525690313055 37.0 33.0 40.0 27.0 40.0 38-39 34.7896991422971 37.0 33.0 40.0 27.0 40.0 40-41 34.70280484952225 37.0 33.0 40.0 27.0 40.0 42-43 34.54900375261777 37.0 33.0 40.0 27.0 40.0 44-45 34.40394207047149 37.0 33.0 40.0 24.5 40.0 46-47 34.25429060444891 37.0 33.0 40.0 22.0 40.0 48-49 34.071232858994414 37.0 33.0 40.0 22.0 40.0 50-51 33.88687784125817 37.0 33.0 40.0 22.0 40.0 52-53 33.68898202652086 37.0 33.0 37.0 22.0 40.0 54-55 33.53933608114147 37.0 33.0 37.0 22.0 40.0 56-57 33.32651189517213 37.0 33.0 37.0 22.0 40.0 58-59 33.08081330430842 37.0 33.0 37.0 22.0 40.0 60-61 32.8332600139341 37.0 33.0 37.0 22.0 40.0 62-63 32.64883535722741 37.0 33.0 37.0 22.0 40.0 64-65 32.33448701631476 37.0 33.0 37.0 22.0 40.0 66-67 32.10640337825507 33.0 30.0 37.0 22.0 38.5 68-69 31.795501101683783 33.0 27.0 37.0 22.0 37.0 70-71 31.642792855530605 33.0 27.0 37.0 22.0 37.0 72-73 31.354534443371687 33.0 27.0 37.0 22.0 37.0 74-75 31.07181576004824 33.0 27.0 37.0 22.0 37.0 76-77 30.876484205203255 33.0 27.0 37.0 18.5 37.0 78-79 30.642908473572128 33.0 27.0 37.0 15.0 37.0 80-81 30.377058273227988 33.0 27.0 37.0 15.0 37.0 82-83 30.215989565037987 33.0 27.0 37.0 15.0 37.0 84-85 30.03157105990513 33.0 27.0 37.0 15.0 37.0 86-87 29.813823800943904 33.0 27.0 37.0 15.0 37.0 88-89 29.629732196753828 33.0 27.0 37.0 15.0 37.0 90-91 29.426594610180288 33.0 27.0 37.0 15.0 37.0 92-93 29.278610220666415 33.0 27.0 37.0 15.0 37.0 94-95 29.031278465816413 33.0 27.0 33.0 15.0 37.0 96-97 28.967328123844116 33.0 27.0 33.0 15.0 37.0 98-99 27.82317403543717 33.0 24.5 33.0 15.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3756409351873806 1101 2 0.3056823907134216 1101 3 0.422365987889048 1101 4 0.39794378989488166 1101 5 0.42957837661177933 1101 6 0.28715480242538405 1101 7 0.2747453060037728 1101 8 0.4320910774875699 1101 9 0.3052286856951696 1101 10-11 0.3606006365219301 1101 12-13 0.313303906197568 1101 14-15 0.47589248129114736 1101 16-17 0.5605555839342244 1101 18-19 0.46914779752127345 1101 20-21 0.5325501379375055 1101 22-23 0.5034743226049443 1101 24-25 0.5891973621288571 1101 26-27 0.6467675164116287 1101 28-29 0.49457912161419415 1101 30-31 0.5288267120386649 1101 32-33 0.6407389630625673 1101 34-35 0.5547271375903122 1101 36-37 0.5722437523483066 1101 38-39 0.48569799512952017 1101 40-41 0.5483968368275285 1101 42-43 0.5545128220316542 1101 44-45 0.5626520004035811 1101 46-47 0.5154149590389565 1101 48-49 0.6376989787574132 1101 50-51 0.5234137954684073 1101 52-53 0.47119055458487935 1101 54-55 0.5931431321181577 1101 56-57 0.5775519737705537 1101 58-59 0.6732916756135978 1101 60-61 0.6719147513190435 1101 62-63 0.5979496154248523 1101 64-65 0.5392491277912939 1101 66-67 0.5817034598932054 1101 68-69 0.4781067965592847 1101 70-71 0.4464512425221798 1101 72-73 0.5700414640975637 1101 74-75 0.6082237348173152 1101 76-77 0.535221858682462 1101 78-79 0.507284436926188 1101 80-81 0.4667774850037425 1101 82-83 0.5955270167983109 1101 84-85 0.469268904508791 1101 86-87 0.5011561076348272 1101 88-89 0.5131861828239721 1101 90-91 0.5183321832305552 1101 92-93 0.6088664471180323 1101 94-95 0.6289223053553812 1101 96-97 0.5273975365884098 1101 98-99 0.5428640225568806 1102 1 0.3506530948974387 1102 2 0.39848446406874416 1102 3 0.36241171405454153 1102 4 0.4472709179501848 1102 5 0.4166478411595236 1102 6 0.4083100842516174 1102 7 0.375970400950564 1102 8 0.36254137226072913 1102 9 0.3390989051596378 1102 10-11 0.3564069192827688 1102 12-13 0.3740798649536927 1102 14-15 0.5247229596293579 1102 16-17 0.48036494201215163 1102 18-19 0.5114937486746669 1102 20-21 0.5036283777704043 1102 22-23 0.44021998081079516 1102 24-25 0.518880267539366 1102 26-27 0.5952129085011464 1102 28-29 0.42255673061431054 1102 30-31 0.47168266613385157 1102 32-33 0.5717178507036067 1102 34-35 0.5035999753015119 1102 36-37 0.5755806186097061 1102 38-39 0.5045650287000498 1102 40-41 0.5186405728926289 1102 42-43 0.5835958470265012 1102 44-45 0.559845359255668 1102 46-47 0.6493685488273897 1102 48-49 0.5732727625805651 1102 50-51 0.5665892912739352 1102 52-53 0.5051093515504022 1102 54-55 0.5160384324817073 1102 56-57 0.510218026380187 1102 58-59 0.4817346875355213 1102 60-61 0.565473867471141 1102 62-63 0.5431863868954139 1102 64-65 0.45332909580842795 1102 66-67 0.45842100334400016 1102 68-69 0.44347083037426316 1102 70-71 0.44441748007251647 1102 72-73 0.4737286643309737 1102 74-75 0.43512308859776283 1102 76-77 0.4446781322985629 1102 78-79 0.4608106925430846 1102 80-81 0.537816666342529 1102 82-83 0.45351327857158097 1102 84-85 0.3774043847212738 1102 86-87 0.45470110808520303 1102 88-89 0.4786010657684656 1102 90-91 0.45992569410397266 1102 92-93 0.6197181670225191 1102 94-95 0.5856820707378496 1102 96-97 0.45589975141056627 1102 98-99 0.4885337393811895 1103 1 0.1513889363789609 1103 2 0.19716944592148167 1103 3 0.16825836479670286 1103 4 0.22948515410460857 1103 5 0.16830666096461044 1103 6 0.18760874089707613 1103 7 0.06177717001166627 1103 8 0.12313321163776436 1103 9 0.13477632346327795 1103 10-11 0.11814343979935416 1103 12-13 0.13540666174569793 1103 14-15 0.21003140813419918 1103 16-17 0.1647027111493813 1103 18-19 0.1820871459619937 1103 20-21 0.21638011764677145 1103 22-23 0.17949868222395082 1103 24-25 0.29802753292879913 1103 26-27 0.3175468657695859 1103 28-29 0.25215217919301125 1103 30-31 0.31678231868027495 1103 32-33 0.38277251176713634 1103 34-35 0.23295586754593245 1103 36-37 0.27125641783798926 1103 38-39 0.21018055257963653 1103 40-41 0.24811998259728085 1103 42-43 0.27255135272825015 1103 44-45 0.17459178969564704 1103 46-47 0.15266168643045575 1103 48-49 0.2833462999554257 1103 50-51 0.27352479332530066 1103 52-53 0.09677747666987813 1103 54-55 0.18527152377099299 1103 56-57 0.1762423416025456 1103 58-59 0.2401633188735559 1103 60-61 0.2021833018849435 1103 62-63 0.1752057403456817 1103 64-65 0.12055934975808924 1103 66-67 0.33906453594233454 1103 68-69 0.19865083583182752 1103 70-71 0.10828921147418313 1103 72-73 0.19535088121110533 1103 74-75 0.17091492361613803 1103 76-77 0.047564162397222276 1103 78-79 0.06494615024720929 1103 80-81 0.06837052529557042 1103 82-83 0.06793041478295336 1103 84-85 0.12295423787627868 1103 86-87 0.2603379420804828 1103 88-89 0.18599601000777 1103 90-91 0.2362145102149924 1103 92-93 0.20853712042239536 1103 94-95 0.15282967988524376 1103 96-97 0.014909358386962168 1103 98-99 0.1753295219108928 1104 1 0.11613562038770908 1104 2 0.03963578400859902 1104 3 0.0017813599250260381 1104 4 0.11666823797337855 1104 5 0.15995450089631902 1104 6 0.09388006816536887 1104 7 -0.023905552540789188 1104 8 0.005169572990432414 1104 9 0.02221560311135562 1104 10-11 0.08903481477213404 1104 12-13 0.04649074184760593 1104 14-15 0.15731112397308067 1104 16-17 0.12934135662433022 1104 18-19 0.09654967814269355 1104 20-21 0.10307344128991502 1104 22-23 0.16092526291565434 1104 24-25 0.09783391159737675 1104 26-27 0.15019147389322995 1104 28-29 0.095611419242978 1104 30-31 0.08361003174430692 1104 32-33 0.19664656731441 1104 34-35 0.09739884650281994 1104 36-37 0.18911824130987043 1104 38-39 0.10109047264383264 1104 40-41 0.02703543044977863 1104 42-43 -0.06062413626761298 1104 44-45 -0.02172044440426646 1104 46-47 -0.054648409726375746 1104 48-49 -0.007231520016091508 1104 50-51 0.03695631386579379 1104 52-53 -0.04664468662526389 1104 54-55 0.11322694242069531 1104 56-57 0.04545876036091556 1104 58-59 0.06168088248195858 1104 60-61 0.1246436965357276 1104 62-63 0.15012663913704216 1104 64-65 0.12500137636038744 1104 66-67 0.09391727837235919 1104 68-69 -0.055110701396557715 1104 70-71 0.01687233818857692 1104 72-73 -0.020310130342338084 1104 74-75 -0.09067242217368943 1104 76-77 -0.1335230309332367 1104 78-79 -0.054500109271685915 1104 80-81 -0.006341893573527102 1104 82-83 0.04079480093567511 1104 84-85 -0.05735619769889411 1104 86-87 0.009537137888305125 1104 88-89 0.13517105608644187 1104 90-91 0.09141067873341768 1104 92-93 0.08372335635760209 1104 94-95 0.05502614836437658 1104 96-97 -0.1277537307868961 1104 98-99 -0.11044750647010204 1105 1 0.007012066668767858 1105 2 0.03410001928379813 1105 3 0.0908495700288654 1105 4 -0.00976941744297477 1105 5 0.026255972670490735 1105 6 0.054081926384874635 1105 7 -0.04399863850552066 1105 8 -0.093849926576965 1105 9 -0.027612077990426087 1105 10-11 -0.0015189664046104667 1105 12-13 -0.036546193323381715 1105 14-15 0.08692962785230662 1105 16-17 0.06993371176180574 1105 18-19 0.016037435836892655 1105 20-21 0.09316515623130073 1105 22-23 0.048607899569375945 1105 24-25 0.12375935648660885 1105 26-27 0.0422109211289694 1105 28-29 0.06452091626642442 1105 30-31 0.05704614478002412 1105 32-33 0.13998682963963205 1105 34-35 0.050457217017211065 1105 36-37 0.00990924914658109 1105 38-39 -0.13213886184112766 1105 40-41 -0.10625073835959853 1105 42-43 -0.0431248514944258 1105 44-45 -0.05749167970703439 1105 46-47 -0.12036235961245723 1105 48-49 0.012610070658240602 1105 50-51 -0.03864205611043303 1105 52-53 -0.13314854241176022 1105 54-55 -0.02763363515239803 1105 56-57 -0.03263511355017101 1105 58-59 -0.07980338277581467 1105 60-61 -0.004351255853201508 1105 62-63 -0.009594341515267502 1105 64-65 -0.1345536390675477 1105 66-67 -0.057619493530467025 1105 68-69 -0.08339741622155472 1105 70-71 -0.20508974934264046 1105 72-73 -0.12416672782061511 1105 74-75 -0.032723078556276874 1105 76-77 -0.1906481612877613 1105 78-79 -0.17181564101001 1105 80-81 -0.10132520068325235 1105 82-83 -0.051554960988305254 1105 84-85 -0.05887683882276207 1105 86-87 -0.05880280458649523 1105 88-89 -0.020246739549030224 1105 90-91 -0.0034742692130933506 1105 92-93 0.03234256415538539 1105 94-95 0.013919880270126583 1105 96-97 -0.07359467656024066 1105 98-99 -0.034073581765944994 1106 1 -0.002262875337603276 1106 2 0.16011957005444089 1106 3 0.035471928782868645 1106 4 -0.028473218772589348 1106 5 0.012540287882011114 1106 6 -0.013793469812874548 1106 7 -0.0365876065313131 1106 8 -0.0757956797494046 1106 9 -0.04022057586605854 1106 10-11 -0.06700910864868348 1106 12-13 -0.042066043507830386 1106 14-15 -0.0326576956289486 1106 16-17 -0.039374612652927965 1106 18-19 -0.014488606783302771 1106 20-21 0.01428567147103621 1106 22-23 -0.037883172115584784 1106 24-25 0.03283397375207642 1106 26-27 0.11785037770997064 1106 28-29 -0.09288550949681706 1106 30-31 -0.038461398077224374 1106 32-33 0.0885445605680033 1106 34-35 -0.03665403725463534 1106 36-37 -0.06579936118037466 1106 38-39 -0.0539873744873276 1106 40-41 0.05129584263033138 1106 42-43 -0.023067892394145417 1106 44-45 -0.020851311051835353 1106 46-47 -0.0034045141770278065 1106 48-49 -0.041732788626312356 1106 50-51 -0.051948987636045274 1106 52-53 -0.14186712440799454 1106 54-55 -0.12411731710987084 1106 56-57 -0.09464299261438214 1106 58-59 -0.08691377921407195 1106 60-61 0.034595903657447025 1106 62-63 -0.04068607369170252 1106 64-65 -0.14651565190217752 1106 66-67 -0.10200418310718362 1106 68-69 -0.0027113314980518055 1106 70-71 -0.05799581410092003 1106 72-73 0.08077735003190156 1106 74-75 -0.12840875112386385 1106 76-77 -0.12420038758199681 1106 78-79 0.015624310133862451 1106 80-81 -0.10265098572719822 1106 82-83 0.00569451636527063 1106 84-85 -0.15110985235460106 1106 86-87 -0.127089983120527 1106 88-89 0.034900429598103244 1106 90-91 -0.032828171489612146 1106 92-93 -0.07345998137289911 1106 94-95 -0.1622439614220994 1106 96-97 -0.3305984698082902 1106 98-99 0.020441672183288517 1107 1 0.0374724692641486 1107 2 0.11338312368040704 1107 3 0.08801735629256413 1107 4 0.030350125681415818 1107 5 0.1538437176885452 1107 6 0.044611326944327345 1107 7 -0.06536152685457353 1107 8 0.014341746860466742 1107 9 -0.030484884199275086 1107 10-11 0.03969229530102325 1107 12-13 -0.03210231180407419 1107 14-15 -0.01354742709693113 1107 16-17 -0.023823571684687295 1107 18-19 -0.07289588115500578 1107 20-21 -0.05711211385602866 1107 22-23 -0.06141531587023508 1107 24-25 -0.03644400838632578 1107 26-27 0.07653625535505881 1107 28-29 -0.007140594895119534 1107 30-31 -0.05222707401384241 1107 32-33 -0.1655533740208952 1107 34-35 -0.016578971704319656 1107 36-37 -0.05779246732249277 1107 38-39 -0.09361272892549977 1107 40-41 -0.24803843727777775 1107 42-43 -0.3055641847286168 1107 44-45 -0.09555760726532014 1107 46-47 -0.19396732829178376 1107 48-49 -0.23658509971726005 1107 50-51 -0.13107578593641733 1107 52-53 -0.20265729676797406 1107 54-55 -0.0026742959150993784 1107 56-57 -0.021678389745538595 1107 58-59 0.015009997901074712 1107 60-61 0.06712000696597187 1107 62-63 -0.20425152653555756 1107 64-65 -0.1992769104809895 1107 66-67 -0.06404984532358782 1107 68-69 -0.16242871070807396 1107 70-71 -0.08504745403495306 1107 72-73 -0.030142058282709883 1107 74-75 -0.1364447494894705 1107 76-77 -0.18736702011264228 1107 78-79 -0.050624534541558575 1107 80-81 -0.21097162518338308 1107 82-83 -0.12606693341772157 1107 84-85 -0.2627431121541939 1107 86-87 -0.31491703321020736 1107 88-89 -0.15403931816044292 1107 90-91 -0.012045055455345732 1107 92-93 -0.10974123284803028 1107 94-95 0.03338845992886519 1107 96-97 -0.11307686162672681 1107 98-99 0.17863052244836908 1108 1 0.024252191048113048 1108 2 -0.015837076832699637 1108 3 0.002020020664122768 1108 4 -0.08226210341370432 1108 5 0.06979510730881344 1108 6 -0.16327260007917488 1108 7 -0.0742508759121705 1108 8 -0.1198113430928558 1108 9 -0.0536578427586889 1108 10-11 -0.15937201352236485 1108 12-13 -0.17605497094135814 1108 14-15 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0.07436034681087378 2216 3 0.14274026912590543 2216 4 0.1969126844769633 2216 5 0.034536675536458006 2216 6 0.054071680635232156 2216 7 0.11832766460926081 2216 8 0.05992947275307614 2216 9 0.09693010042943229 2216 10-11 0.005057390578933507 2216 12-13 0.08634849369600062 2216 14-15 -0.030151476244761 2216 16-17 0.0441883743321867 2216 18-19 -0.02466496750169256 2216 20-21 -0.043224408793257396 2216 22-23 -0.20159154683328495 2216 24-25 -0.038397011755940014 2216 26-27 -0.09046600536738936 2216 28-29 0.039506421359256194 2216 30-31 0.0011256289172152378 2216 32-33 0.011650549553273493 2216 34-35 -0.16207420223408064 2216 36-37 -0.09080527560526974 2216 38-39 0.004014045223762253 2216 40-41 -0.07916446487326567 2216 42-43 -0.06436309622527858 2216 44-45 -0.10233958878210814 2216 46-47 0.06110385241949956 2216 48-49 0.10953303946666182 2216 50-51 0.1836499482574041 2216 52-53 0.27990335463592686 2216 54-55 0.19007333339050803 2216 56-57 0.28072532513385084 2216 58-59 0.0450130108761968 2216 60-61 0.04435389510629051 2216 62-63 0.1084208543771723 2216 64-65 0.2978883077606582 2216 66-67 0.1360188262269375 2216 68-69 0.060034530252135454 2216 70-71 0.06165994561059307 2216 72-73 0.052044828932416465 2216 74-75 0.16639734583650068 2216 76-77 0.11506105956006607 2216 78-79 0.102162996036256 2216 80-81 0.3100543448781501 2216 82-83 0.194955324110051 2216 84-85 0.2690175728813813 2216 86-87 0.16723757271857664 2216 88-89 0.14462685807184172 2216 90-91 0.27489498584422023 2216 92-93 0.34170976905705075 2216 94-95 0.35431825513080284 2216 96-97 0.3186069628099908 2216 98-99 0.4097007298893942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 6 7.0 7 179.0 8 1030.0 9 2016.0 10 3585.0 11 7542.0 12 14719.0 13 26041.0 14 35148.0 15 39036.0 16 42322.0 17 44317.0 18 47209.0 19 50691.0 20 55262.0 21 60927.0 22 68049.0 23 76472.0 24 86303.0 25 98668.0 26 110101.0 27 124885.0 28 147150.0 29 176956.0 30 216460.0 31 271216.0 32 346389.0 33 447417.0 34 589689.0 35 805309.0 36 1089775.0 37 1026864.0 38 228014.0 39 93.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.022274233079095 18.45072528645668 12.323181752076044 25.20381872838818 2 16.25509220183913 20.80414950469578 37.101182821461926 25.839575472003162 3 18.29515490697947 26.006094478454255 28.43732417726197 27.261426437304305 4 13.153168983259992 17.366019116252286 35.667124774895775 33.813687125591954 5 14.80138066554035 37.23541331714786 32.809008932558406 15.154197084753388 6 32.44906299700576 36.01369498067854 17.461936348245956 14.075305674069744 7 28.538602151063408 31.514560065465364 21.561629069246376 18.385208714224852 8 27.584950474310006 32.61409868165464 19.790401683575347 20.010549160460016 9 27.462896940159855 15.347545782299587 18.74390856174469 38.44564871579587 10-11 25.78564667473522 25.435913929071724 26.304650227032507 22.47378916916055 12-13 26.607354979407212 23.3517922610362 25.86322748472714 24.17762527482945 14-15 24.787680006485967 24.379404783179893 23.115776878316034 27.717138332018106 16-17 24.65658839078141 26.555003508763075 24.30967117314141 24.478736927314106 18-19 25.219252344025662 25.94533522212939 25.262912744972628 23.572499688872323 20-21 26.33190566320019 25.361010054470817 24.114049673204264 24.193034609124727 22-23 25.36137735946375 25.183636308860113 24.031643695796156 25.423342635879987 24-25 24.920747641910108 25.829769862540914 23.9895787703845 25.259903725164477 26-27 25.258559405057447 25.590160885789054 23.935245043288955 25.216034665864544 28-29 24.79986761953678 25.452825736074498 24.71004703355447 25.03725961083425 30-31 25.3466251081164 25.518412577158262 24.340072940665973 24.794889374059366 32-33 24.63926328722727 25.74612902205632 23.962597785416772 25.65200990529964 34-35 25.06382064209059 25.74041527089614 24.581458120969188 24.614305966044082 36-37 25.234773238003918 25.730597975564372 24.08344310212196 24.95118568430975 38-39 25.361519432906736 25.326699936701864 24.078450572640726 25.23333005775068 40-41 25.03946076881108 25.776269467956688 24.42010769670722 24.76416206652501 42-43 25.62740138120183 25.512753395550458 24.188390529036926 24.671454694210784 44-45 25.053246603503148 25.78018123798373 24.053189977477356 25.11338218103577 46-47 24.610838032057902 25.719745968392584 24.82189222095633 24.847523778593185 48-49 25.360522512762966 25.838122371181793 24.313924388461132 24.487430727594102 50-51 24.43624374806876 26.16175232154876 24.28113733451675 25.12086659586573 52-53 24.49977057784257 25.723334386461744 24.098806263437837 25.67808877225785 54-55 24.919718018164808 25.582573758553252 24.23895173396304 25.258756489318895 56-57 25.062505510784895 25.077087895421986 24.0559029792703 25.804503614522822 58-59 24.753128666791486 25.11444687650684 24.697283417675617 25.43514103902606 60-61 24.81138722564178 25.338829475376436 24.456607034782103 25.393176264199685 62-63 24.162877270896857 25.520916060828654 24.320696686241817 25.995509982032672 64-65 24.220662631760007 25.13687646109737 24.58307708347891 26.05938382366372 66-67 24.924246887361562 25.081530073676166 24.394864317127322 25.599358721834946 68-69 25.0366925605863 24.65706947540167 24.336272786651904 25.969965177360127 70-71 24.153160938894207 24.82866683880558 24.713742820995037 26.304429401305175 72-73 24.879314796696008 24.827326111175342 24.68887626677073 25.60448282535792 74-75 25.15154559869877 24.505583026451294 24.712039308241327 25.630832066608612 76-77 24.558084343124694 24.796307983118187 25.131292724849093 25.514314948908023 78-79 24.73542317543926 24.689120752397418 25.14574892335628 25.429707148807047 80-81 24.24573423844541 24.539266836502684 25.48174315412642 25.733255770925485 82-83 24.42811262932304 24.845149902024357 24.789572798434534 25.93716467021807 84-85 24.471985969364216 24.883226566723046 24.614631502588153 26.03015596132458 86-87 24.465739755933942 24.30753389556615 24.905947956738977 26.32077839176093 88-89 24.544369488130695 23.98644540139098 25.21503299530698 26.25415211517134 90-91 24.917549194057077 24.22992153904175 24.926248150387366 25.926281116513806 92-93 24.41481576588435 24.454461870573727 24.88456729435328 26.246155069188642 94-95 23.7802178319614 24.58797468264583 25.420282622229802 26.21152486316297 96-97 24.589622988967704 24.37722018580592 24.94987650321199 26.083280322014385 98-99 24.338883262214306 24.427978556560014 24.813406203720252 26.41973197750543 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 565.0 1 571.0 2 610.5 3 618.5 4 545.0 5 484.0 6 414.0 7 378.5 8 393.0 9 398.5 10 392.5 11 369.5 12 368.0 13 385.5 14 416.5 15 479.0 16 576.0 17 718.0 18 896.0 19 1142.0 20 1567.5 21 2242.0 22 3347.5 23 4756.0 24 6479.0 25 8866.0 26 12261.5 27 16161.0 28 21026.0 29 27338.0 30 33493.5 31 40559.0 32 48282.0 33 55616.0 34 62710.5 35 71149.5 36 79407.0 37 88409.0 38 99411.0 39 103845.0 40 108331.0 41 118101.0 42 132488.0 43 153964.0 44 174471.5 45 194757.0 46 227926.0 47 259022.0 48 281734.5 49 309740.0 50 338095.5 51 326428.5 52 300380.0 53 297058.5 54 287765.5 55 277855.5 56 257385.0 57 240557.0 58 229741.5 59 204130.5 60 172724.0 61 135315.0 62 109937.0 63 89310.0 64 67136.0 65 52071.0 66 37499.0 67 27363.5 68 21348.5 69 18479.5 70 16695.5 71 14983.5 72 14616.0 73 13202.0 74 9680.5 75 6906.0 76 5013.5 77 3459.0 78 2201.0 79 1421.5 80 976.5 81 671.5 82 432.5 83 305.0 84 231.0 85 163.5 86 109.0 87 83.0 88 57.0 89 37.5 90 23.5 91 14.0 92 12.5 93 11.5 94 9.5 95 8.0 96 8.0 97 5.5 98 4.0 99 10.0 100 16.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0021924840070910293 2 0.0 3 6.309306495225984E-5 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.864450228325916E-4 22-23 0.0 24-25 0.0044480610791343194 26-27 3.154653247612992E-5 28-29 0.008470243969840884 30-31 4.810846202609813E-4 32-33 3.94331655951624E-5 34-35 5.520643183322736E-5 36-37 0.0 38-39 3.154653247612992E-5 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 2.365989935709744E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.94331655951624E-5 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.0 92-93 0.0 94-95 0.0 96-97 0.0 98-99 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 99 6339841.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.61061646816644 #Duplication Level Percentage of deduplicated Percentage of total 1 77.81811424434409 34.715140488303945 2 12.31868295586636 10.990880814741862 3 4.077281127830035 5.456700738795564 4 1.8035384027386099 3.218278398807265 5 0.9587784837985712 2.1385849609334096 6 0.6084463503966284 1.6285900067399761 7 0.41553224408723183 1.2976004699792416 8 0.2905251205433273 1.0368403781540958 9 0.21597026902328242 0.8671110155931941 >10 1.2443189386543996 10.398609532688724 >50 0.11582459435530491 3.6252907175029963 >100 0.10791211096374129 10.032135492203565 >500 0.014567827335257709 4.5179170120822665 >1k 0.010151149383351009 8.486200183555212 >5k 2.849445440887727E-4 0.835832197265073 >10k+ 7.123613602219318E-5 0.7542875926538463 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 24309 0.3834323289811211 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 23162 0.3653403926060606 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 9746 0.1537262527561811 No Hit GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG 7157 0.11288926646583093 No Hit GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC 7052 0.11123307351083411 No Hit CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC 6934 0.10937182809474244 No Hit TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG 6527 0.10295210873585 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.577326623806496E-5 0.0 5 0.0 3.154653247612992E-5 0.0 3.154653247612992E-5 0.0 6 0.0 3.154653247612992E-5 0.0 3.154653247612992E-5 0.0 7 0.0 3.154653247612992E-5 0.0 3.154653247612992E-5 0.0 8 0.0 3.154653247612992E-5 0.0 3.154653247612992E-5 0.0 9 0.0 3.154653247612992E-5 0.0 4.7319798714194886E-5 0.0 10-11 0.0 3.154653247612992E-5 0.0 6.309306495225984E-5 0.0 12-13 7.88663311903248E-6 3.154653247612992E-5 0.0 1.1041286366645473E-4 0.0 14-15 1.577326623806496E-5 3.154653247612992E-5 0.0 1.1041286366645473E-4 0.0 16-17 1.577326623806496E-5 3.154653247612992E-5 0.0 1.3407276302355216E-4 0.0 18-19 3.154653247612992E-5 4.7319798714194886E-5 0.0 1.97165827975812E-4 0.0 20-21 3.154653247612992E-5 6.309306495225984E-5 0.0 3.233519578803317E-4 0.0 22-23 3.154653247612992E-5 6.309306495225984E-5 0.0 0.0012382013996880994 0.0 24-25 3.9433165595162406E-5 1.2618612990451968E-4 0.0 0.003367592341826869 7.88663311903248E-6 26-27 7.097969807129233E-5 1.2618612990451968E-4 0.0 0.005615282780751126 1.577326623806496E-5 28-29 7.88663311903248E-5 1.2618612990451968E-4 0.0 0.01405398021811588 1.577326623806496E-5 30-31 9.463959742838977E-5 1.2618612990451968E-4 0.0 0.04135750407620632 1.577326623806496E-5 32-33 1.0252623054742225E-4 1.2618612990451968E-4 0.0 0.0897183383621135 1.577326623806496E-5 34-35 1.2618612990451968E-4 1.2618612990451968E-4 0.0 0.15226722562916012 1.577326623806496E-5 36-37 1.2618612990451968E-4 1.2618612990451968E-4 0.0 0.24745888737588215 1.577326623806496E-5 38-39 1.2618612990451968E-4 1.2618612990451968E-4 0.0 0.39182370661977173 1.577326623806496E-5 40-41 1.3407276302355216E-4 1.2618612990451968E-4 0.0 0.565029943179963 1.577326623806496E-5 42-43 1.4195939614258465E-4 1.3407276302355216E-4 0.0 0.7231727104828023 1.577326623806496E-5 44-45 1.4195939614258465E-4 1.4195939614258465E-4 0.0 0.9041867138308358 2.365989935709744E-5 46-47 1.4195939614258465E-4 1.4195939614258465E-4 0.0 1.1020150189886466 3.9433165595162406E-5 48-49 1.4984602926161714E-4 1.4195939614258465E-4 0.0 1.3144178221504292 4.7319798714194886E-5 50-51 1.7350592861871457E-4 1.4195939614258465E-4 0.0 1.5488164450811936 4.7319798714194886E-5 52-53 1.7350592861871457E-4 1.4195939614258465E-4 0.0 1.7815825349563184 4.7319798714194886E-5 54-55 1.7350592861871457E-4 1.7350592861871457E-4 0.0 2.014112025837872 4.7319798714194886E-5 56-57 1.7350592861871457E-4 2.050524610948445E-4 0.0 2.2751122622791327 4.7319798714194886E-5 58-59 1.7350592861871457E-4 2.365989935709744E-4 0.0 2.5600878634022526 4.7319798714194886E-5 60-61 1.7350592861871457E-4 2.365989935709744E-4 0.0 2.8496219384681734 6.309306495225984E-5 62-63 1.7350592861871457E-4 2.365989935709744E-4 0.0 3.143217629590395 6.309306495225984E-5 64-65 1.7350592861871457E-4 2.6814552604710433E-4 0.0 3.4499682247551635 6.309306495225984E-5 66-67 1.7350592861871457E-4 2.6814552604710433E-4 0.0 3.783430846294095 6.309306495225984E-5 68-69 1.7350592861871457E-4 2.6814552604710433E-4 0.0 4.141176411206527 6.309306495225984E-5 70-71 1.7350592861871457E-4 2.6814552604710433E-4 0.0 4.5551221237251855 6.309306495225984E-5 72-73 1.97165827975812E-4 2.6814552604710433E-4 0.0 4.955116382256274 9.463959742838977E-5 74-75 2.050524610948445E-4 2.6814552604710433E-4 0.0 5.347705092288592 9.463959742838977E-5 76-77 2.1293909421387698E-4 2.6814552604710433E-4 0.0 5.771690488767778 9.463959742838977E-5 78-79 2.2082572733290946E-4 2.996920585232343E-4 0.0 6.213215757303693 9.463959742838977E-5 80-81 2.2082572733290946E-4 2.996920585232343E-4 0.0 6.695294093337672 9.463959742838977E-5 82-83 2.2082572733290946E-4 2.996920585232343E-4 0.0 7.206710704574452 9.463959742838977E-5 84-85 2.2082572733290946E-4 3.0757869164226676E-4 0.0 7.713324356241742 9.463959742838977E-5 86-87 2.2082572733290946E-4 3.154653247612992E-4 0.0 8.23232790853903 9.463959742838977E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACTAG 1785 0.0 21.882547 1 GTACATA 1520 0.0 21.720589 1 GTACAAG 3110 0.0 21.2317 1 ATGGGTA 3680 0.0 20.090734 5 CATGGGT 5275 0.0 18.687876 4 CATGGGG 14695 0.0 18.22634 4 TAGTACT 2525 0.0 17.678947 4 GTATAAG 2125 0.0 17.506039 1 CGTTAAC 560 0.0 17.437654 1 TACATGA 2130 0.0 16.80961 2 TCTAACG 895 0.0 16.625454 2 GTATATA 1415 0.0 16.431242 1 GTACATG 57960 0.0 16.358583 1 ATTGCGT 1055 0.0 16.307817 6 TACATGG 56555 0.0 16.131493 2 GTATTAT 1395 0.0 16.000143 1 TAGGCAT 3025 0.0 15.986542 5 CTAGTAC 2545 0.0 15.895638 3 GTATTAG 2780 0.0 15.890429 1 CTAACGC 940 0.0 15.829554 3 >>END_MODULE