FastQCFastQC Report
Fri 27 May 2016
ERR765455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6339841
Sequences flagged as poor quality0
Sequence length99
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT250280.39477330740628985No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA239420.3776435402717513No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138740.21883829578691325No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98280.15501966058770245No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93590.14762199872204998No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC77810.12273178459838345No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC74000.11672217016168071No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG70480.11116998044588185No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69930.11030245080278828No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG68130.10746326287993657No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4900.035.1130642
AGTACTT225500.022.1392612-13
GTATAAC8600.021.6413841
TCACGCA8450.021.462044
GTATCAA493000.020.9050061
ATCACGC9800.019.9290373
GTACTAG17650.019.7715191
TACTTTT257200.019.48291414-15
GAGTACT216650.019.1690412-13
TAACGCA4051.8189894E-1218.3707984
CAACGCA572350.017.8659735
ATCAACG579050.017.667283
AACGCAG589450.017.5046276
TCAACGC588450.017.4166684
GTACTTT244050.017.04547514-15
TATCAAC607050.016.9902672
TAGTACT25850.016.5497074
ACGCAGA627100.016.3127967
CATGGGG128200.016.1774444
CGCAGAG633950.016.1365348
Produced by FastQC (version 0.11.5)