FastQCFastQC Report
Fri 27 May 2016
ERR765454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15522562
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA257040.16559122134606388No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT247890.15969657586163932No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194470.12528215380940336No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG50300.022.7938231
GTATAAG62950.021.1244811
GTATCAA580100.020.8902591
GTACATG946300.019.8472631
TACATGG930200.019.5237962
CATGGGG365650.019.5196574
ACATGGG909450.018.739523
CTATTGA64600.018.4731929
GTGATCG45200.017.8784878
CAACGCA644850.017.8009555
ATCAACG651300.017.6900523
AATACGG25300.017.6433735
AACGCAG672700.017.3977496
TAATACG29800.017.3442844
GTATAAT42050.017.2087781
TGATCGC47150.017.1390829
TATCAAC709400.017.022882
TAGGCAT87350.016.94445
GTATTAT40950.016.523571
GGGCTAT80000.016.502786
Produced by FastQC (version 0.11.5)