##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR765454_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	15522562
Sequences flagged as poor quality	0
Sequence length	100
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.04115100329443	33.0	33.0	33.0	27.0	33.0
2	31.96280182356495	33.0	33.0	33.0	27.0	33.0
3	31.987301709601805	33.0	33.0	33.0	27.0	33.0
4	35.76801059000441	37.0	37.0	37.0	33.0	37.0
5	35.81245061221208	37.0	37.0	37.0	33.0	37.0
6	35.82057910285686	37.0	37.0	37.0	33.0	37.0
7	35.75711277558434	37.0	37.0	37.0	33.0	37.0
8	35.74732921021671	37.0	37.0	37.0	33.0	37.0
9	35.739975076279286	37.0	37.0	37.0	33.0	37.0
10-11	35.7094668393014	37.0	37.0	37.0	33.0	37.0
12-13	35.66168635693	37.0	37.0	37.0	33.0	37.0
14-15	37.314012886532524	40.0	37.0	40.0	33.0	40.0
16-17	37.350909115389584	40.0	37.0	40.0	33.0	40.0
18-19	37.31745258933415	40.0	37.0	40.0	33.0	40.0
20-21	37.40403085521578	40.0	37.0	40.0	33.0	40.0
22-23	37.388050149195735	40.0	37.0	40.0	33.0	40.0
24-25	37.367209710613494	40.0	37.0	40.0	33.0	40.0
26-27	37.322350749831116	40.0	37.0	40.0	33.0	40.0
28-29	37.28072092738299	40.0	37.0	40.0	33.0	40.0
30-31	37.21416226264711	40.0	37.0	40.0	33.0	40.0
32-33	37.118382906120786	40.0	37.0	40.0	33.0	40.0
34-35	37.0197012580784	40.0	37.0	40.0	33.0	40.0
36-37	36.94388010819348	40.0	37.0	40.0	33.0	40.0
38-39	36.85077679187238	37.0	37.0	40.0	33.0	40.0
40-41	36.759568362490675	37.0	37.0	40.0	33.0	40.0
42-43	36.630984949520574	37.0	37.0	40.0	33.0	40.0
44-45	36.49260244539529	37.0	37.0	40.0	33.0	40.0
46-47	36.32468283908288	37.0	37.0	40.0	33.0	40.0
48-49	36.09074600571736	37.0	37.0	40.0	33.0	40.0
50-51	35.99437550966135	37.0	37.0	40.0	27.0	40.0
52-53	35.83688485186917	37.0	37.0	40.0	27.0	40.0
54-55	35.676528043502095	37.0	37.0	40.0	27.0	40.0
56-57	35.510715048198875	37.0	33.0	40.0	27.0	40.0
58-59	35.334613996065855	37.0	33.0	40.0	27.0	40.0
60-61	35.14232102922185	37.0	33.0	40.0	27.0	40.0
62-63	34.91879594360776	37.0	33.0	37.0	27.0	40.0
64-65	34.710561729436165	37.0	33.0	37.0	27.0	40.0
66-67	34.50588353262818	37.0	33.0	37.0	27.0	40.0
68-69	34.29799330162121	37.0	33.0	37.0	27.0	40.0
70-71	34.098047281112486	37.0	33.0	37.0	27.0	40.0
72-73	33.88635960996645	37.0	33.0	37.0	27.0	37.0
74-75	33.67276184820521	37.0	33.0	37.0	27.0	37.0
76-77	33.48700456148927	37.0	33.0	37.0	27.0	37.0
78-79	33.33120866259062	37.0	33.0	37.0	27.0	37.0
80-81	33.1739026714791	37.0	33.0	37.0	27.0	37.0
82-83	33.05415330278597	37.0	33.0	37.0	27.0	37.0
84-85	32.92104173911497	37.0	33.0	37.0	27.0	37.0
86-87	32.80943461523942	37.0	33.0	37.0	27.0	37.0
88-89	32.700536097069545	33.0	33.0	37.0	27.0	37.0
90-91	32.58568218313446	33.0	33.0	37.0	27.0	37.0
92-93	32.49295464241019	33.0	33.0	37.0	22.0	37.0
94-95	32.39259092667821	33.0	33.0	37.0	22.0	37.0
96-97	32.308159793467084	33.0	33.0	37.0	22.0	37.0
98-99	32.20761063798618	33.0	33.0	37.0	22.0	37.0
100	32.152123212650075	33.0	33.0	37.0	22.0	37.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	13.0
7	196.0
8	1149.0
9	2105.0
10	2899.0
11	4334.0
12	7176.0
13	12595.0
14	20349.0
15	26585.0
16	30896.0
17	37230.0
18	45675.0
19	55830.0
20	66209.0
21	79350.0
22	94580.0
23	99087.0
24	101147.0
25	112303.0
26	130802.0
27	157699.0
28	196885.0
29	253327.0
30	331286.0
31	434617.0
32	580621.0
33	778733.0
34	1039925.0
35	1449355.0
36	2450740.0
37	4577183.0
38	2338972.0
39	2708.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.260337760508314	18.829325516370943	12.526061946600443	25.3842747765203
2	16.37642505643402	20.816451017564738	37.431851709732705	25.375272216268534
3	18.245253473945848	25.81141202123619	29.108678446153963	26.834656058663995
4	13.183072505806779	17.074054453105518	36.52617459038473	33.21669845070298
5	14.384912041348146	37.115996213328216	33.48629677524473	15.012794970078899
6	31.783471053296488	36.92917444942401	17.38993215166414	13.89742234561537
7	27.56672513210126	31.53239136683751	22.404716437917916	18.49616706314331
8	26.709965790441036	32.89407380044609	20.282598967876567	20.11336144123631
9	27.042327162230052	14.473081183376815	19.757762925991212	38.72682872840193
10-11	25.566823311770314	25.273286072234725	27.230836636374846	21.929053979620118
12-13	26.420245575440447	23.12294194734091	26.898829587538447	23.557982889680197
14-15	24.162586691552594	23.901476444416843	24.524379416232964	27.411557447797602
16-17	23.886587355569354	26.378624399597435	25.275345430781716	24.459442814051496
18-19	24.26519109505736	25.864428862705342	26.7786296632181	23.091750379019192
20-21	25.467800021407673	25.144707142802613	25.692551601721412	23.6949412340683
22-23	24.87974163287492	24.85027982277043	25.688118167306616	24.58186037704803
24-25	24.439557289003176	25.38201339601019	25.859241225629432	24.3191880893572
26-27	24.517330973792046	25.316085694694657	25.675000246429814	24.491583085083484
28-29	24.190932787118484	25.352502788809296	26.094739743780078	24.36182468029214
30-31	24.700878382209666	25.133245787219128	25.888827174176388	24.27704865639482
32-33	24.291641444754106	25.29878583672695	25.542675153900646	24.8668975646183
34-35	24.73540146338126	25.187207314406358	25.954057905631096	24.123333316581288
36-37	24.684630841342337	25.11350229243559	25.657389356909633	24.54447750931244
38-39	24.73916226886959	24.83890244439142	25.869346878082244	24.552588408656746
40-41	24.605924981832295	24.955127687529792	26.05513973166895	24.38380759896896
42-43	24.906891693201	24.922014282502726	25.732883397104438	24.438210627191832
44-45	24.517823291859962	25.22001911280884	25.53252317008451	24.72963442524669
46-47	24.37316604530746	25.051328179466925	25.782603316808146	24.792902458417473
48-49	24.961257791288187	25.116210518448455	25.454462280307254	24.468069409956097
50-51	24.268058567105083	25.38845109766692	25.292603743172126	25.05088659205587
52-53	24.244597422888383	25.28377799204698	25.24867445901734	25.2229501260473
54-55	24.416660660435028	25.3286047724201	25.198122679136514	25.056611888008355
56-57	24.48750770431363	24.906523786629396	25.138835500807126	25.46713300824985
58-59	24.23311671860386	24.918340997838406	25.57500862756175	25.273533655995983
60-61	24.27668918143976	25.117256031037343	25.342834392318913	25.263220395203984
62-63	24.05720563961982	25.094388654571997	25.247877745348003	25.600527960460184
64-65	23.88009912974281	24.93078110981075	25.434908729190887	25.754211031255558
66-67	24.468096964665783	24.91612687980395	25.27861326767769	25.33716288785258
68-69	24.36397816779389	24.77810045452462	25.24799419433122	25.609927183350273
70-71	23.889452598275764	24.823423734651392	25.396565741675726	25.89055792539712
72-73	24.476885978671277	24.8837844752643	25.307302447460195	25.332027098604225
74-75	24.425272157278197	24.89454483278861	25.34058596447532	25.339597045457868
76-77	24.111703088925847	24.977519768655114	25.42251463542467	25.48826250699437
78-79	24.281718867537	25.03531827811733	25.30184178184514	25.38112107250053
80-81	23.939947574455278	24.913591523471794	25.588263754296747	25.55819714777618
82-83	24.134847640132797	24.859101540561117	25.45528672330779	25.55076409599829
84-85	24.230395001311354	24.890338974282624	25.0495694829924	25.82969654141362
86-87	23.815511243015443	24.748317610414894	25.409902628124215	26.026268518445445
88-89	23.990653106500798	24.377072673118064	25.543012051021957	26.089262169359184
90-91	24.188795900539958	24.50382702532905	25.34829185460567	25.959085219525324
92-93	24.00446632956813	24.617114575739116	25.257915747441974	26.12050334725078
94-95	23.57009634379593	24.595256102625754	25.61200847683413	26.222639076744187
96-97	23.93841029319431	24.491999721175954	25.335310398552174	26.234279587077562
98-99	23.692765039973356	24.60254138624576	25.351539125076144	26.353154448704736
100	23.718380191714697	24.59814413287986	25.20007431920873	26.483401356196712
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	925.0
1	899.0
2	1220.5
3	1595.5
4	1339.5
5	1156.0
6	1531.5
7	2952.0
8	4646.0
9	4311.5
10	2684.5
11	1896.5
12	1925.5
13	2148.5
14	2475.0
15	2938.0
16	3446.5
17	4089.0
18	4954.0
19	5943.0
20	7293.5
21	9139.0
22	11512.5
23	14701.5
24	18517.0
25	23812.5
26	32240.5
27	42158.0
28	53099.0
29	70982.0
30	91010.0
31	108781.0
32	132793.5
33	158568.0
34	184369.5
35	207396.5
36	229539.0
37	260313.5
38	278287.5
39	286488.0
40	301404.5
41	315302.5
42	331857.5
43	369710.0
44	419596.0
45	468825.5
46	528862.0
47	584657.0
48	639615.0
49	687259.5
50	724803.5
51	730174.0
52	709708.0
53	710644.5
54	697965.0
55	664755.0
56	633019.5
57	583994.0
58	539673.0
59	495993.0
60	422481.0
61	343077.0
62	278719.5
63	221025.5
64	167983.0
65	132259.5
66	106660.5
67	81418.0
68	62465.0
69	50852.5
70	43795.0
71	37517.0
72	31621.5
73	31077.5
74	24953.5
75	18374.0
76	14364.0
77	10619.5
78	8389.5
79	5991.0
80	4247.0
81	3213.5
82	2697.5
83	2361.5
84	1820.5
85	1155.0
86	789.0
87	435.0
88	209.0
89	158.5
90	121.0
91	79.5
92	55.5
93	39.5
94	27.0
95	22.0
96	13.5
97	8.0
98	7.5
99	7.5
100	9.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.01808979728990614
2	0.005772243009884579
3	0.006100797020491849
4	0.0039877437758019585
5	0.04567544970991258
6	0.0
7	0.0
8	0.0
9	0.0
10-11	0.0
12-13	0.0
14-15	0.0
16-17	3.8653413012619954E-4
18-19	0.0016814234660489681
20-21	5.830223129403509E-4
22-23	0.00497984804312587
24-25	0.007405349709667772
26-27	0.0058044541873950965
28-29	0.021864947294138685
30-31	0.020112659237566582
32-33	0.012865144297700342
34-35	0.013322543018349677
36-37	0.0132742262520839
38-39	0.020866400791312672
40-41	0.029898414965261536
42-43	0.017606629627248387
44-45	0.016772360129726008
46-47	0.018179988586935585
48-49	0.013026200185252924
50-51	0.009846956964964933
52-53	0.026934986634294004
54-55	0.02549514699957391
56-57	0.01311639148228237
58-59	0.016324624762329827
60-61	0.017252306674632706
62-63	0.020731113845768503
64-65	0.01089059911630567
66-67	0.015416269556533259
68-69	0.007605059010232976
70-71	0.00879365146037104
72-73	0.01040421033589687
74-75	0.003626978587684172
76-77	0.007389244120912515
78-79	0.00550166911879624
80-81	0.0023063203097529908
82-83	2.3192047807571973E-4
84-85	0.0026477587913644666
86-87	0.002380406018027179
88-89	0.001195034685640167
90-91	8.21385026518174E-4
92-93	0.0012658992761633034
94-95	0.0031792432202879914
96-97	0.0014688296944795583
98-99	0.0018392582358504996
100	0.0021259377156940974
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.5522562E7
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.10792870971946
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.8458266893881	23.124506507036816
2	14.833952141482797	9.822428599672056
3	5.637966061250204	5.599841352710685
4	2.7575693188255648	3.651896336791458
5	1.569345352189267	2.597888702060591
6	1.039219745305615	2.0643847944786664
7	0.697329162164276	1.6160986931700274
8	0.5090655002478939	1.3483283432627955
9	0.398945904770436	1.188744531675671
>10	2.2910699443313103	14.176354016733935
>50	0.21374963537090433	4.939806061334882
>100	0.16474686047458018	11.104124458694276
>500	0.022579372479503645	5.290916079139802
>1k	0.017522126658068796	10.701386237639436
>5k	9.951129499691372E-4	2.1169308834707903
>10k+	1.1707211176107498E-4	0.6563644021280823
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA	25704	0.16559122134606388	No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT	24789	0.15969657586163932	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	19447	0.12528215380940336	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	6.4422355021033255E-6	0.0
3	0.0	0.0	0.0	6.4422355021033255E-6	0.0
4	0.0	0.0	0.0	1.9326706506309976E-5	6.4422355021033255E-6
5	0.0	0.0	6.4422355021033255E-6	1.9326706506309976E-5	6.4422355021033255E-6
6	0.0	0.0	6.4422355021033255E-6	1.9326706506309976E-5	6.4422355021033255E-6
7	0.0	0.0	6.4422355021033255E-6	1.9326706506309976E-5	6.4422355021033255E-6
8	6.4422355021033255E-6	0.0	6.4422355021033255E-6	1.9326706506309976E-5	6.4422355021033255E-6
9	6.4422355021033255E-6	0.0	6.4422355021033255E-6	3.865341301261995E-5	6.4422355021033255E-6
10-11	6.4422355021033255E-6	0.0	6.4422355021033255E-6	3.865341301261995E-5	9.663353253154988E-6
12-13	1.6105588755258314E-5	0.0	6.4422355021033255E-6	5.798011951892993E-5	2.8990059759464967E-5
14-15	2.8990059759464967E-5	0.0	6.4422355021033255E-6	7.086459052313658E-5	5.1537884016826604E-5
16-17	3.221117751051663E-5	0.0	6.4422355021033255E-6	9.019129702944656E-5	6.442235502103326E-5
18-19	3.865341301261995E-5	6.4422355021033255E-6	6.4422355021033255E-6	1.0307576803365321E-4	6.442235502103326E-5
20-21	5.1537884016826604E-5	1.2884471004206651E-5	6.4422355021033255E-6	1.6749812305468648E-4	7.73068260252399E-5
22-23	5.1537884016826604E-5	1.2884471004206651E-5	6.4422355021033255E-6	4.7994654490669776E-4	9.341241478049822E-5
24-25	7.086459052313658E-5	1.2884471004206651E-5	6.4422355021033255E-6	0.001385080632952215	1.0951800353575653E-4
26-27	9.663353253154988E-5	1.2884471004206651E-5	6.4422355021033255E-6	0.002502808492567142	1.0951800353575653E-4
28-29	1.1596023903785985E-4	1.2884471004206651E-5	6.4422355021033255E-6	0.01094535811807355	1.0951800353575653E-4
30-31	1.1596023903785985E-4	1.2884471004206651E-5	6.4422355021033255E-6	0.043153314510839126	1.0951800353575653E-4
32-33	1.1596023903785985E-4	1.2884471004206651E-5	6.4422355021033255E-6	0.10907348928611141	1.1596023903785985E-4
34-35	1.1596023903785985E-4	1.6105588755258314E-5	6.4422355021033255E-6	0.19637222257511355	1.288447100420665E-4
36-37	1.3206582779311818E-4	1.9326706506309976E-5	6.4422355021033255E-6	0.33668733292867503	1.288447100420665E-4
38-39	1.3528694554416982E-4	1.9326706506309976E-5	6.4422355021033255E-6	0.5666332658230001	1.288447100420665E-4
40-41	1.4495029879732483E-4	2.5768942008413302E-5	6.4422355021033255E-6	0.840582888314442	1.288447100420665E-4
42-43	1.6105588755258314E-4	2.5768942008413302E-5	6.4422355021033255E-6	1.0833971866242185	1.3528694554416982E-4
44-45	1.6105588755258314E-4	2.5768942008413302E-5	6.4422355021033255E-6	1.3617500770813478	1.3528694554416982E-4
46-47	1.642770053036348E-4	3.221117751051663E-5	6.4422355021033255E-6	1.677500144628187	1.385080632952215E-4
48-49	1.6749812305468645E-4	3.865341301261995E-5	6.4422355021033255E-6	2.0116524578867843	1.5783476980153147E-4
50-51	1.7071924080573812E-4	3.865341301261995E-5	6.4422355021033255E-6	2.3643068715074227	1.7071924080573812E-4
52-53	1.739403585567898E-4	3.865341301261995E-5	6.4422355021033255E-6	2.6965844942349078	1.900459473120481E-4
54-55	1.739403585567898E-4	4.5095648514723274E-5	6.4422355021033255E-6	3.0297318187551774	2.0937265381835806E-4
56-57	1.739403585567898E-4	5.1537884016826604E-5	6.4422355021033255E-6	3.415402689324095	2.1903600707151306E-4
58-59	1.739403585567898E-4	5.1537884016826604E-5	6.4422355021033255E-6	3.842758044709372	2.1903600707151306E-4
60-61	1.739403585567898E-4	5.1537884016826604E-5	6.4422355021033255E-6	4.285149577756559	2.254782425736164E-4
62-63	1.739403585567898E-4	5.4759001767878265E-5	6.4422355021033255E-6	4.72914844856152	2.254782425736164E-4
64-65	1.7716147630784146E-4	5.798011951892993E-5	6.4422355021033255E-6	5.173559622438615	2.254782425736164E-4
66-67	1.900459473120481E-4	5.798011951892993E-5	6.4422355021033255E-6	5.654533703907899	2.3514159582677138E-4
68-69	1.9648818281415144E-4	5.798011951892993E-5	6.4422355021033255E-6	6.191194469057363	2.3836271357782304E-4
70-71	1.9970930056520308E-4	5.798011951892993E-5	6.4422355021033255E-6	6.784959209697471	2.4480494907992635E-4
72-73	1.9970930056520308E-4	6.120123726998159E-5	6.4422355021033255E-6	7.369685493928129	2.609105378351847E-4
74-75	1.9970930056520308E-4	6.442235502103326E-5	6.4422355021033255E-6	7.939546319737682	2.86679479843598E-4
76-77	1.9970930056520308E-4	6.442235502103326E-5	6.4422355021033255E-6	8.546804322636946	3.1566953960306295E-4
78-79	2.0615153606730642E-4	6.764347277208492E-5	6.4422355021033255E-6	9.19191690134657	3.221117751051663E-4
80-81	2.0615153606730642E-4	7.086459052313658E-5	6.4422355021033255E-6	9.900350212806366	3.285540106072696E-4
82-83	2.0615153606730642E-4	7.73068260252399E-5	6.4422355021033255E-6	10.645484939921644	3.478807171135796E-4
84-85	2.0615153606730642E-4	7.73068260252399E-5	6.4422355021033255E-6	11.361867969991035	3.478807171135796E-4
86-87	2.0615153606730642E-4	7.73068260252399E-5	6.4422355021033255E-6	12.085959134838696	3.5110183486463124E-4
88	2.0615153606730642E-4	8.374906152734323E-5	6.4422355021033255E-6	12.649039507782286	3.543229526156829E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTACTAG	5030	0.0	22.793823	1
GTATAAG	6295	0.0	21.124481	1
GTATCAA	58010	0.0	20.890259	1
GTACATG	94630	0.0	19.847263	1
TACATGG	93020	0.0	19.523796	2
CATGGGG	36565	0.0	19.519657	4
ACATGGG	90945	0.0	18.73952	3
CTATTGA	6460	0.0	18.473192	9
GTGATCG	4520	0.0	17.878487	8
CAACGCA	64485	0.0	17.800955	5
ATCAACG	65130	0.0	17.690052	3
AATACGG	2530	0.0	17.643373	5
AACGCAG	67270	0.0	17.397749	6
TAATACG	2980	0.0	17.344284	4
GTATAAT	4205	0.0	17.208778	1
TGATCGC	4715	0.0	17.139082	9
TATCAAC	70940	0.0	17.02288	2
TAGGCAT	8735	0.0	16.9444	5
GTATTAT	4095	0.0	16.52357	1
GGGCTAT	8000	0.0	16.50278	6
>>END_MODULE