FastQCFastQC Report
Fri 27 May 2016
ERR765453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR765453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15358830
Sequences flagged as poor quality0
Sequence length100
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA612140.39855900482002865No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT543270.353718349639914No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG253290.1649149056275771No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG239570.15598193351967565No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG214170.1394442154773508No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205020.13348673043454481No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC181420.1181209766629359No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT172980.11262576641580122No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC170500.11101106008725925No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC160020.10418762366664648No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA155200.10104936378617381No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACTAG63750.019.7398071
GTACATA38950.019.6502651
TACATGA54200.019.491982
CATGGGG402500.018.7816054
CGTTAAC18500.018.5284611
GTACAAG61250.018.0156541
TAGTACT70050.017.829794
TATTCGC11300.017.4521059
GTATAAG69750.017.3685591
TAGGCAT93800.017.169755
GTGATCG51050.016.5557738
TGATCGC53550.016.0460669
ATGGGGG213250.015.9412455
ATTGCGT40400.015.9225246
GTACATG1577250.015.8141471
CTAGGCA107900.015.6223274
ATAAGGT61800.015.5753613
CTATTGA61450.015.3585829
TACATGG1558250.015.3525252
CTAGTAC90250.015.2437963
Produced by FastQC (version 0.11.5)